Objective To explore the significance of the changes of high density lipoprotein cholesterol (HDL-C) and high sensitivity C reactive protein (hs-CRP) in coronary heart disease patients combined with type 2 diabetes mellitus,and analyze the relationship between HDL-C and hs-CRP.Methods Two hundred and ten elder patients were selected.The patients were divided into simple coronary heart disease group (A group,70 cases),coronary heart disease combined with type 2 diabetes mellitus group (B group,71 cases),and simple type 2 diabetes mellitus group (C group,69 cases) according to the pathogenetic condition.Meanwhile 50 healthy controls were selected as control group (D group).The levels of HDL-C and hs-CRP were measured,and correlation analysis used Pearson linear correlation.Results The levels of HDL-C in A,B and C group were significantly lower than those in D group:(1.98 ± 0.47),(0.99 ± 0.31) and (1.27 ± 0.44) mmol/L vs.(2.18 ± 0.42) mmol/L,and there were statistical differences (P ＜ 0.05 or ＜0.01);the level of HDL-C in B group was significantly lower than that in A and C group,and there were statistical differences (P ＜ 0.05).The levels of hs-CRP in A,B,and C group were significantly higher than that in D group:(5.71 ± 1.81),(7.71 ± 2.32) and (4.68 ± 2.09) mg/L vs.(1.60 ± 0.80) mg/L,and there were statistical differences (P＜ 0.01);the level of hs-CRP in B group was significantly higher than that in A and C group,and there were statistical differences (P ＜ 0.05).Correlation analysis results showed that hs-CRP and HDL-C had signficant negative correlation (r =-0.304,P ＜ 0.01),and hs-CRP and LDL-C had signficant positive correlation (r =0.341,P ＜ 0.01),hs-CRP and triacylglycerol (TG) had no correlation (r =0.006,P ＞ 0.05);hs-CRP and fasting plasma glucose (FPG) had signficant positive correlation (r =0.415,P ＜ 0.01),and hs-CRP and glycosylated hemoglobin (HbA1c) had signficant positive correlation (r =0.384,P ＜ 0.01).Conclusions The levels of HDL-C in patients with elder patients with simple coronary heart disease and simple type 2 diabetes mellitus are significantly lower,and the levels of hs-CRP are significantly higher.The level of HDL-C in elder coronary heart disease patients combined with type 2 diabetes mellitus is significantly lower than that in elder patients with simple coronary heart disease and simple type 2 diabetes mellitus,and the level of hs-CRP is significantly higher than that in elder patients with simple coronary heart disease and simple type 2 diabetes mellitus.The level of hs-CRP is HDL-C have signficant negative correlation.

Objective To explore multiple recurrence of Kawasaki disease (KD).Methods The clinical data of one child with multiple recurrent KD in two years was retrospectively analyzed.Results A four-year boy had his ifrst episode of classic KD without coronary artery abnormality at 2 years old. He had since had four episodes incomplete KD within 2 years, all of which were manifested by fever accompanied with neck lymph node swelling, bilateral conjunctival congestion, red and chap lips and strawberry-like tongue. White blood cell count, C reaction protein, and erythrocyte sedimentation rate were all increased. No purulent changes of cervical lymph nodes were detected by ultrasound. After intravenous infusion with high doses of gamma globulin, the fever was brought down, and the duration of fever was 6~8 d. The fever free intervals between 2 adjacent episode were about 2.5, 1.5, 4.5, and 13.5 months. The clinical manifestations and genetic test results were not consistent with the periodic fever, aphthous stomatitis, pharyngitis, and cervical lymphadenitis syndrome (PFAPA).Conclusion Five episodes of KD within 2 years are rare and a long-term follow-up is warranted.

Objective This article aims to provide mechanisms and recent developments of molecular biology pertaining to re-sistance of Enterococci,providing rapid approaches for detecting resistant strains.Methods This article reviewed recent lit-eratures on resistance of Enterococci and a systemic analysis was conducted.Results Common detecting methods include polymerase chain reaction (PCR),pulsed field gel electrophoresis (PFGE),multilocus sequence typing (MLST)and South-ern blot.There also exist less widely-used methods such as pyrosequencing and genechip technique,which may prove effi-cient in some aspects.Conclusion Every method has its advantages and disadvantages.This article discussed how to utilize these methods to achieve their maximum capabilities.

Calcitonin participates in physiological regulation of calcium metabolism, but it might not be the key factor.Multiple immunoassay methods have been developed for serum calcitonin detection.However, significant differences exist among the methods, and the results of immunoassay are affected by many factors.This paper mainly discusses some major advances in experimental researches of calcitonin and the influence factors of immunological measurement.

Objective:To explore the reading feature of developmental dyslexia. Methods:820 children of grade 3~5 in 5 primary schools in Wuhan City were selected by cluster sampling; fifty-five children with developmental dyslexia were screened out according to ICD-10. One hundred and ten normal children with the same age, sex and socioeconomic status were chose as control. Results:The prevalence rate of developmental dyslexia is 6.7%. The score of 8 items of DCCC (Dyslexia Checklist for Chinese Children) of developmental dyslexics were mostly significantly higher than that of normal children (15.2?3.7/10.1?3.3, 21.6?3.7/13.4?4.9, 16.1?3.5/10.6?3.7, 16.8?3.6/11.7?3.1, 15.9?3.9/11.5?3.5, 21.4?4.3/14.5?4.6, 14.9?3.7/9.4?2.8, 23.2?4.4/15.9?4.7, all withP

Kawasaki disease is a disease based on systemic vasculitis, which has become the most common disease in children with acquired heart disease, but its etiology and pathogenesis remain unclear.The homeostasis of the gut microbiota plays an important role in the maturation of the body′s immune system and immune regulation.The disorder of the gut microbiota is involved in a variety of inflammatory and immune diseases in children.Studies have found that there are changes in the composition of gut microbiota in children with Kawasaki disease.Gut microbiota disorders are related to the occurrence and development of Kawasaki disease, with the immune system function affected.Various mechanisms such as intestinal mucosal barrier dysfunction, immune inflammation, oxidative stress and metabolite effects promote the pathogenesis of Kawasaki disease.This article reviews the research on the relationship between gut microbiota and Kawasaki disease.

Objective To discuss which drainage method is more efficient in treatment of choledocholithiasis with obstructive jaundice. Methods Compared the difference of serum total bilirubin,direct bilirubin, the variation of serum total bilirubin and direct bilirubin before and after drainage and daily biliary drainage by endoscopic nasobil-iary drainage (ENBD) and endoscopic nasobiliary drainage with continuous negative-pressure suction after three days. Results The daily biliary drainage about the patients by ENBD with continuous negative-pressure suction is much more effective than the patients by ENBD. The serum total bilirubin and direct bilirubin about the patients by ENBD with continuous negative-pressure suction is less than the patients by ENBD after three days. The variation of serum total bilirubin and direct bilirubin before and after drainage about the patients by ENBD with continuous neg-ative-pressure suction is more than the patients by ENBD. Conclusion The ENBD with continuous negative-pres-sure suction is more effective for choledocholithiasis with obstructive jaundice.

We collected 22 cultivated population of Lonicera japonica from 17 areas. The characteristics of non-glandular hairs were observed and measured by the scanning electron microscopy. The principal components analysis and correlation analysis were conduct based on length and density of L. japonica. The results showed a significant negative correlation between length and density of non-glandular hairs, and the characteristics of non-glandular was not corrrelated significantly with latitude. The correlation results indicated that the density was a key to separate "Damaohua" and "Jizhuahua". The contribution of climate and soil was important to the cultivated population. This reminded that the characteristics of non-glandular hairs were affected by environmental and genetic interaction.
Lonicera ; anatomy & histology ; growth & development ; ultrastructure

Sixty-three morphological traits from 743 specimens of the 41 taxa within the cultivated Lonicera japonica were observed and measured, such as the height of plants, the length of leaf, the width of leaf, the length of anther, the alabastrum's number of one branch, the color of alabastrum and so on. A numerical taxonomy is presented by using the cluster analysis, principal components analysis (PCA) and factor analysis. Sixteen of 63 characters were screened by means of PCA and used for cluster analysis of 41 taxa with the method of Ward linkage and average euclidean distance. The cluster analysis showed that the 41 taxa could be divided into 5 groups when the Euclidean distance coefficient was 11.84. The factor analysis indicated that the shape of leaf, color of alabastrum, the pilosity and color of twiggery were of significance for the cultivated L. japonica classification. The results of this study will be a base for the core collection and breeding of L. japonica.
Breeding ; China ; Flowers ; chemistry ; classification ; genetics ; Lonicera ; chemistry ; classification ; genetics ; growth & development ; Plant Leaves ; chemistry ; classification ; genetics ; Quantitative Trait Loci

Objective To analyze the characteristics of newly added N-glycosylation mutation in major hydrophilic region (MHR) of HBV S gene in patients with coexistence of HBsAg and antiHBs, and reveal the generation mechanism and clinical implications of the coexistence. Methods HBV S genes from 284 patients with HBsAg+antiHBs and 314 patients with single HBsAg were amplified respectively for sequence analysis. A chronic hepatitis B (CHB) patient with HBsAg+antiHBs in MHR was found to harbor a novel double N-glycosylation mutation and selected for further study. Recombinant vectors harboring the novel mutant or control PreS/S genes were constructed and transfected in HepG2 cells respectively for phenotypic analysis, and the effects of the mutations on HBV duplication and antigenicity were investigated. Results The detection rate of MHR N-glycosylation mutation was significantly higher in HBsAg+antiHBs group than in single HBsAg group (11.3% vs. 2.9%, P<0.01, respectively). In HBsAg+antiHBs cohort, the proportion of hepatocellular carcinoma (HCC) patients accounted for 46.9%(15/32) in patients with N-glycosylation mutation at the time of testing; by contrast, the number was 22.6%(57/252) in patients with non-N-glycosylation mutation (P<0.01). N-glycosylation mutational pattern of the novel strain was s116-118TST → NST+s131-133TSM → NST concomitant with sP120 deletion+G145D mutation. The novel mutants accounted for 98.0%, 2.0% and 2.5%, respectively, of viral clones in three sequential serum samples. Mutants with single N-glycosylation mutation s130-132GTS→NSS without sP120 deletion+G145D were detected in sample 2, accounting for 17.6% of viral clones. Compared to the wild-type, the novel mutant had an increase of 31% in replication capacity, but a decrease of 99% in HBsAg level. Immunofluorescence showed that elimination of the two additional N-glycosylation mutations only partly restored HBsAg detection by antiHBs, suggesting that sP120 deletion+G145D mutation also attenuated HBsAg antigenicity. Conclusions Additional N-glycosylation mutation in MHR of HBV S gene is associated with coexisting HBsAg+antiHBs, and the two parameters together might be a better risk factor for HCC occurrence. Combination of two additional N-glycosylation mutation, sP120 deletion and sG145D mutation may co-play a role in silence of HBsAg antigenicity.