1.Progress of the relationship between Henoch-Sch?nlein purpura nephritis and IgA nephropathy in children
International Journal of Pediatrics 2016;43(1):14-17
Scholars have focused on the relationship between Henoch-Sch?nlein purpura nephritis and IgA nephropathy in children, when they gradually recognizes the similarities and differences between Henoch-Sch?nlein purpura nephritis and IgA nephropathy in their incentives, genetic factors, renal immunopathology, therapy and so on,with aberrantly glycosylated IgA1 involved in the pathogenesis of Henoch-Sch?nlein purpura nephritis and IgA nephropathy. This paper will review their differences and similarities,along with their relation, according to the research progress.
2.Anterior cervical microsurgical discectomy combined with cage and anterior locking titanium plate for cervical spondylosis: retrospective analysis of 108 cases
Chinese Journal of Tissue Engineering Research 2017;21(15):2349-2354
BACKGROUND: Anterior cervical discectomy combined with cage and anterior locking titanium plate is widely used for the treatment of various kinds of cervical spondylosis. Microsurgical technique has increasingly become a standard technique.OBJECTIVE:To explore the clinical effects of anterior cervical microsurgical discectomy combined with cage and anterior locking titanium plate for cervical spondylosis. METHODS: Clinical data of 108 patients treated with anterior cervical microsurgical discectomy combined with cage and anterior locking titanium plate were retrospectively analyzed. According to the clinical outcome before and after surgery,including the scores of Japanese Orthopedic Association, visual analogue scale of neck and upper extremities, neck disability index, imaging manifestations (disc space height and Cobb angle) and other indexes, the surgical results were evaluated.RESULTS AND CONCLUSION: Compared with pre-operation, the postoperative Japanese Orthopedic Association score, visual analogue scale score of neck and upper extremities, neck disability index score, disc space height and Cobb angle were markedly improved, with a significant difference (P < 0.05). The improvement-rate of the spinal function included 102 excellent cases and 6 good cases. Complete fusion of bone graft observed in all cases. It suggested that anterior cervical microsurgical discectomy combined with cage and anterior locking titanium plate has significant effect on various types of cervical spondylosis.
3.Effect of RNA silencing of transforming growth factor ?1 on the growth of colon cancer cells by RNA interference
Zhihua RAN ; Zhigang HUANG ; Wei LU
Chinese Journal of Digestion 2001;0(03):-
Objectives To observe the effect of inhibition of transforming growth factor (TGF)?1 on the growth and aggression of colon cancer cells by RNA interference. Methods HCT116 colon cancer cells were transfected with small interfering dsRNA (siRNA), the effect of the inhibition of TGF?1 was tested by semi-quantitative RT-PCR, and changes in cell cycle distribution were analyzed by flow cylometry, the ability of aggression was detected by soft agar colony assay. Results The inhibition of the expression of TGF?1 was significantly detected after the transfection of siRNA against TGF?1, which induced the increase of S phase by 59.0%-82. 5% and decrease in G2 phase, colony assay further demonstrated that siRNA against TGF?1 led to a significant reduction in colony formation as compared with the control group. Conclusions The present data suggested that the expression of TGF?1 was conveniently and rapidly blocked by siRNA transfection, and this may lead to the cell cycle arrest and inhibition of the growth and tumorigenesis of colon cells.
4.The influence of diet on gastrointestinal microbiota
Ying RAN ; Lu ZHOU ; Bangmao WANG
Chinese Journal of Clinical Nutrition 2013;21(5):305-308
The gastrointestinal tract harbors a complex microbiota,which contains almost 30 genera,400 to 500 species.The factors influencing human intestinal microbiota include host and environmental ones,with the most important environmental factor being diet.Dietary fiber,fat,and protein have different impact on gastrointestinal microbiota and the microbiota in turn plays an important role in maintaining health.Its variation can lead to many diseases.Knowledge of the interaction between diet and gastrointestinal microbiota may be conducive to reaching a better understanding of some diseases,and to discovering better preventive and therapeutic strategies.
5.Role of Th17 Cells and Intestinal Flora in Pathogenesis of Inflammatory Bowel Disease
Zelan LU ; Yuqi QIAO ; Zhihua RAN
Chinese Journal of Gastroenterology 2015;(10):619-621
Inflammatory bowel disease(IBD)is an autoimmune disease and its etiology has not yet been clarified. Dysregulated immune responses resulted from complex interactions among genetic factors,intestinal flora and environmental cues have been considered as the etiology of IBD. Recently,the relationship between Th17 cells and IBD has become a hotspot of study,and more and more studies showed that Th17 cells and their related cytokines regulated by intestinal flora contributed to the pathogenesis of IBD. This article reviewed the role of Th17 cells and intestinal flora in the pathogenesis of IBD.
6.Reproductive Toxicity of 3, 4-dichloroaniline on Sperms of Rats
Journal of Environment and Health 1992;0(02):-
0.05);the body weight of 170 and 357 mg/kg 3,4-DCA exposure groups decreased significantly(P
7.Rhabdomyolysis induced by simvastatin-diltiazem interaction in unrecognized hypothyriodism
Ran ZHANG ; Haihong RAN ; Caiyi LU ; Wei GAO ; Ya HUANG ; Yuling GAO ; Qiongxiang YANG
Journal of Geriatric Cardiology 2010;07(2):126-128
Simvastatin,a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor,is widely prescribed to patients with hypercholesteremia and its muscular toxicity has been widely reported.The metabolism of simvastatin depends on the enzymic activity of cytochrome P450 3A4 (CYP3A4) and inhibitors of CYP3A4 can result in clinical events by interacting with simvastatin.Diltiazem is a moderate inhibitor of CYP3A4,which is known to increase the serum concentration of simvastatin.Here we report a patient with unrecognized hypothyroidism who had been stable for more than one year on low-dose simvastatin therapy of hypercholesteremia and rhabdomyolysis occurred with the addition of diltiazem.This is one of scanty reports of rhabdomyolysis induced by simvastatindiltiazem drug interaction,especially in hypothyroid patient.This case reminds the clinicians that although diltiazem as a moderate CYP3A4 inhibitor can be used cautiously with small doses of CYP3A4-dependent statius (eg,simvastatin),these two commonly used drugs should be avoided in hypothyroid patient.
8.Culture and identification of rabbit marrow stromal cells invitro
Zhongshi XU ; Deming XIAO ; Bowen LIN ; Xiaohu LU ; Ran LI
Chinese Journal of Tissue Engineering Research 2008;12(51):10197-10200
BACKGROUND: The study of isolation, purification, culture, cell labeling, inducing factors, effects of gene transfection on cytobiology, cell carrier construction, and time window for back transplantation of cell compound pertaining to marrow stromal cells (MSCs) is still in its infancy. OBJECTIVE: To search for an in vitro culture method that can be simply and effectively obtained. DESIGN, TIME AND SETTING: The present cytological in vitro experiment was performed at the Beijing Institute of Genome, Chinese Academy of Sciences between June 2006 and July 2007. MATERIALS: Eight specific pathogen-free New Zealand rabbits, aged 6 weeks, were provided by the Laboratory Animal Center, Institute of Genetics and Development, Chinese Academy of Sciences. METHODS: Under sterile condition, 1 mL rabbit bone marrow was taken and diluted with D-Hanks solution. Following centrifugation and subsequent supernatant removal, bone marrow was re-suspended using dulbecco's modified eagle's medium (DMEM) for single cell suspension. Next, single cell suspension was dropped onto the liquid surface of equal-volume lymphocyte separation medium (density: 1.077). Subsequent to centrifugation, cloudlike mononuclear cell layer was collected and re-suspended with DMEM containing 20% fetal bovine serum. The cells were inoculated at lxl0/cm2 and purified by adherent method. When 70%-80% of flask bottom was covered, cell digestion and passage was performed.MAIN OUTCOME MEASURES: Cell growth was observed with an operating microscope. Surviving cells were counted by Trypan blue viability test. Cell identification was performed by hematoxylin-eosin staining. Through the use of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, cell viability was detected to observe the cellular resuscitation of the cultured cells following cryopreservation. RESULTS: Twenty-four hours after inoculation, cells began to adhere to the wall, exhibiting short shuttle- or triangle-shaped appearance with different sizes of cellular processes. Three days later, adherent cells began to divide, and cell clusters could be found in some areas; One week later, most of cells exhibited scattered fibroblast-like growth; After passage, cells were evenly distributed with long shuttle-shaped appearance and arranged orderly. Following 3 passages, there wound be 5×106-1×107 adherent cells in 1 mL MSC suspension. Approximately 90% of MSCs survived and identified as mononuclear cells. Cells vigorously grew at days 1-6 after inoculation and reached a peak level at day 8, followed by a viability decline. After 56 days of resuscitation, frozen cells displayed a good and rapid growth. CONCLUSION: Highly purified MSCs can be acquired by gradient centrifugation of lymphocyte separation medium. Enough seeded cells can be obtained by in vitro culture and the cellular viability does not change after frozen preservation and resuscitation.
9.Feasibility of SOX-9 eukaryotic expression plasmid in transfection of rabbit bone marrow stromal cells
Zhongshi XU ; Deming XIAO ; Bowen LIN ; Xiaohu LU ; Ran LI
Chinese Journal of Tissue Engineering Research 2008;12(47):9384-9388
BACKGROUND: SOX-9 plays an important role in occurrence and development of cartilaginous tissues,enhances agglutination of mesenchymal cells,has structural domains of transcriptional activation,and can directly regulate the transcription,OBJECTIVE: To construct pDC316-SOX-9 plasmid for transfection of rabbit bone marrow stromal cells (BMSCs) using SOX-9 gene,and to investigate the effects of SOX-9 gene on growth characteristics of BMSCs and product expression. DESIGN,TIME AND SETTING: The cell gene engineering in vitro experiment was performe,d at the Beijing Institute of Genome, Chinese Academy of Scienees from June 2006 to January 2007.MATERIALS: Eight SPF New Zealand rabbits aged 6 weeks were used for culture of BMSCs.METHODS: pDC316-SOX-9 plasmid was used for transfection of rabbit BMSCs by liposome mediated method,Cell transfection included a SOX-9 plasmid transfection group,a blank plasmid group and a blank control group (only treatment of liposome). MAIN OUTCOME MEASURES: Cell morphology; growth activity; The SOX-9 protein expression in rabbit BMSCs were detected by immunohistochemistry,hematoxylin-eosin staining,reserve transcriptase-polymcrase chain reaction (RT-PCR) and enzyme-labeled immunosorbent assay (ELISA). RESULTS: Some cell colonies were detected at 4 days after pDC316-SOX-9 plasmid transfection.Spindle-shaped cells were collected after clone amplification.Cells in the blank control group gradually died over time.Cell activities in the SOX-9 plasmid transfeetion group and the blank plasmid group significantly prolonged,reached a peak at 2 weeks of transfection,and then gradually decreased.At 6 days,BMSCs were yellow in the SOX-9 plasmid transfection group following immunohistochemistry,expressing SOX-9 protein.Hematoxyliu-eosin staining showed many dikaryocytes,rich cell proliferation,similar to chondroblasts.No SOX-9 protein expression and unproductive cell proliferation in the blank plasmid group.SOX-9 mRNA was detected in the SOX-9 plasmid transfection group,but SOX-9 mRNA was not detected in the blank plasmid group and blank control group.SOX-9 levels were significantly higher in the SOX-9 plasmid transfection group than in the blank plasmid group and blank control group at 24,48 and 72 hours,1,2 weeks (P< 0.01).CONCLUSION: Rabbit BMSCs were successfully transfected with pDC316-SOX-9 plasmid to enhance cell growth activity and to persistently stably secrete SOX-9 protein,resulting in the differentiation of BMSCs into cartilages.
10.Extraction Technology of Total Flavonoids for the Bone Healing Formulation
Ying GAO ; Shuhui ZHAO ; Yang LU ; Ran WEN
China Pharmacy 2015;(22):3125-3127
OBJECTIVE:To optimize the extraction technology of total flavonoids for Bone healing formulation in order to use it for preparation research. METHODS:With the content of total flavonoids as the index,L9(34)orthogonal test was employed to investigate the effects of volume fraction of the solvent ethanol,the amount of solvent,extraction times and extraction time on the extraction of total flavonoids for Bone healing formulation to determine the optimal levels of the factors,and verification tests were conducted. RESULTS:The optimal extraction technology of total flavonoids was 1 h reflux extraction for 3 times,with 70% etha-nol 10 times as much as the amount of medicinal materials. Verification tests showed the average content of total flavonoids was 62.03 mg/ml(RSD=0.84%,n=3),that is to say,6.20 g total flavonoids might be extracted from 100 g medicinal materials for the formulation. CONCLUSIONS:The optimal technology is stable and feasible and can be used for the extraction of total flavo-noids for Bone healing formulation and provide a experimental basis for the preparation of Bone healing liniment.