Objective To get genetic transformated Erigeron breviscapus plantlet with the antiinsect and antifungi effects.Methods The plasmid with gene RBBI2-3 was produced by PCR,middle carrier PBS and plant carrier YY46.By using the particle gun to bombard E. breviscapus,genetic transformation was realized.It was done with the control experiment on bombarded materials,bombarding pressures,and Gent.sensitivity.Results The result of genetic transformation by using true leaves as bombarded material was the best;Ⅱ-type callus were the next in order;the roots,stems,and Ⅰ-type callus were the last in the result.The bombarding pressure of 8.96?10~6 Pa was the best to induce true leaves forming adventitious bud.The result of using the pressure of 7.58?10~6 Pa and 8.96?10~6 Pa to bombard Ⅱ-type callus did not show any difference and was next to true leaves.The best result is using 50 ?g/mL Gent to choose the E.breviscapus of genetic transformation.Conclusion The paddy RBBI2-3 transformed into the genetic transformation on E.breviscapus by particle gun bombarding is the best.The genetic transformated E.breviscapus plantlet has been obtained.

Humans ; Lymphoproliferative Disorders ; classification ; World Health Organization

Humans ; Multiple Myeloma

Humans ; Multiple Myeloma ; diagnosis ; therapy ; Prognosis

China ; Humans ; Multiple Myeloma ; diagnosis ; therapy ; Translational Medical Research

Objective To explore the effects of continuous positive airway pressure (CPAP) on N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP) in the non‐obese patients with obstructive sleep apnea (OSA) .Methods A total of 41 cases of severe OSA complicating coronary artery disease(CAD) were divided into two groups based on their body mass index (BMI):obese group (BMI>28 kg/m2 ) and non‐obese group (BMI≤28 kg/m2 ) .All the patients were treated by CPAP for 24 weeks .Serum NT‐proBNP lev‐el was detected by adopting the electrochemiluminescence method ,the changes of the serum levels of NT‐proBNP were compared between before and after treatment .Results The NT‐proBNP level after treatment in the non‐obese group was significantly de‐creased ,which was decreased from (141 .8 ± 156 .5)pg/mL before treatment to (106 .7 ± 167 .2)pg/mL ,the difference was statisti‐cally significant (P<0 .05);the NT‐proBNP level after treatment in the obese group showed the increasing trend ,which was in‐creased from (32 .2 ± 24 .5)pg/mL before treatment to (86 .3 ± 174 .4)pg/mL ,the difference was not statistically significant (P>0 .05) .Conclusion CPAP can effectively decrease the NT‐proBNP level in non‐obese patients OSA complicating CAD .

Objective To investigate the effect of perioperative anesthesia management on postoperative complications of laparoscopic operation for elderly patients,and to provide reference for the clinical anesthesia man-agement.Methods 90 elderly patients who received laparoscopic surgery were randomly divided into observation group and control group,45 cases in each group.The control group was treated with the routine intervention of the perioperative anesthesia management,and the observation group was given to strengthen the perioperative anesthesia management.The intubation,skin incision,withdrawal of blood pressure,heart rate changes before surgery,and abnor-mal blood pressure,chills,hypoxemia,ischemic heart disease,incidence of complications after surgery were compared between the two groups.Results Before surgery,the withdrawal of the systolic blood pressure,diastolic blood pres-sure,heart rate between the two groups had no statistically significant differences (P >0.05 ).In the observation group,during tracheal intubation for systolic blood pressure (136.92 ±19.57 )mmHg,diastolic blood pressure (84.57 ±12.39)mmHg,after skin incision systolic blood pressure (126.93 ±16.68)mmHg,diastolic blood pressure (78.43 ±11.76)mmHg,which were significantly higher than those in the control group (P <0.05).In the observa-tion group,during tracheal intubation the heart rate was (74.46 ±11.28)/min,which was significantly lower than the control group (P <0.05),abnormal blood pressure of the patients in the observation group was 8 cases,2 cases of shivering,hypoxemia in 1 case,the incidence rate of postoperative complications was 24.44%,abnormal blood pres-sure after surgery in the control group had 15 cases,4 cases of shivering,hypoxemia in 5 cases,ischemic heart disease 2 cases,the incidence rate of postoperative complications was 57.78%.The incidence of postoperative complications in the observation group was significantly lower than that of the control group (χ2 =14.58,P <0.05).Conclusion To strengthen the perioperative anesthesia management helps to reduce the blood pressure and heart rate fluctuations during perioperative period,and can reduce the incidence of postoperative complications and improve the safety of anesthesia.

It is generally known that postoperative pain can cause many adverse clinical effects on the patients of orthopaedic surgery such as lack of exercises of the involved limb,atrophy of the related muscles,anchylosis,osteoparosis,et al,which will ultimately affect the patient's final recovery and living quality.This paper introduces some recent advanced theories about the orthopaedic postoperative pain diagnosis,evaluation and various kinds of treatments hoping to achieve more effective perioperative analgesia in orthopaedic surgery.

Objective:To explore effect of objective life status and psychological factors on the life satisfaction (SLS) in retired carders.Methods:Two hundred and forty-four retired carders were assessed with Quality of Life Inventory (QOLI), The Elderly's Coping Style Questionaire and Social Support Questionaire.Results:The correlation analysis showed that SLS was significantly related with OIQL, coping style, social support,and life expectation.Multiple stepwise regression analysis revealed that objective indicators about physical state and mental state from OIQL distancing style from positive coping and social support had significant effect on SLS.