Glycogen storage diseases (GSD) are a group of inherited disorders characterized by enzyme defects that affect the glycogen metabolism. Hypoglycemia, hepatomegaly, splenomegaly and growth retardation are the main clinical manifestations. The enzymes affecting glycogen synthesis and degradation are varied.Consequently, the clinical manifestations are different because of different enzyme defection. Most patients treated regularly can obtain the normal growth and grow to adults, but still can be accompanied long-term complications by adulthood. Now some scientists find new modified cornstarch is superior to standard therapy in maintaining blood glucose levels. Gene therapy is still in the experimental animals.

Triple negative breast cancer(TNBC) is defined by the lack of estrogen receptor (ER) , progesterone receptor (PR) and human epidermal growth factor receptor-2 ( Her-2) expression. It is a subtype of tumor with distinct clinical and pathologic features and lacks effective targeted therapies. It is non - sensitive to endocrine therapy and targeted therapy drug herceptin, leaving chemotherapy as the mainstay of treatment The feature and the treatment of TNBC is the hotspot of resent research.

ObjectiveTo observe the improve effect of Yiqihuoxue Chinese materia medica for the ethology of mouse with diabetes mellitus encephalopathy induced by streptozotocin (STZ).MethodsCreating mouse model using 1% STZ intraperitoneal injection. 50 mouses were randomly divided into the normal control group, model control group, large dosis Chinese materia medica group, middle dosis Chinese materia medica group and small dosis Chinese materia medica group. Mouses'behaviors of evading dark reaction and Morris water maze in different groups were tested.ResultsYiqihuoxue Chinese materia medica had not the fuction of lower blood suga to the mouse with diabetes mellitus encephalopathy induced by STZ. But Yiqihuoxue chinese materia medica could improve the ethology show and mental status of mouse with diabetes mellitus encephalopathy induced by STZ obviosly, decrease the lightning stroke frequency when the diabetes mellitus encephalopathy mouses entered the dark chamber, extend the latency period, shorten the diabetes mellitus encephalopathy mouses swimming out time and distance, improve the study and memory ability.ConclusionYiqihuoxue Chinese materia medica had the improve effect for the ethology of mouse with diabetes mellitus encephalopathy induced by STZ.

Hypoglycemia is very common in daily blood glucose management of diabetic patients, and growing number of studies have proved that hypoglycemia plays an important role in the pathogenesis of cognitive dysfunction. There may be a bidirectional relationship between severe hypoglycemia and cognitive dysfunction, resulting in a vicious circle of mutual aggravation between hypoglycemia and cognitive dysfunction, but the specific mechanism is not clear. This article reviews the relationship between hypoglycemia and cognitive dysfunction, the mechanism of hypoglycemia induced cognitive dysfunction, and clinical management measures. The aim is to improve the understanding of the relationship between hypoglycemia and cognitive dysfunction, and to provide scientific basis for further exploring the mechanism of diabetes mellitus with cognitive dysfunction.

Open ocular trauma in children is one of the main causes of non-congenital monocular blindness in children. Children’s psychological and daily activities are different from those of adults; the eye tissue structure is not maturely enough, and the characteristics of high response to injury and incompatibility with various diagnostic and treatment methods determine the factors that cause injuries and injuries in children with open ocular trauma. The environment, diagnosis and treatment, and prognosis of children are more complicated than adults. For the emergency of first and second stage on diagnosis and treatment of children with open ocular trauma, it should follow the principles of adult open ocular trauma management, combined with the children's characteristics, clarifying the anatomical location of the ocular trauma before surgery, and closing the wound during the first-stage operation. At the same time, the disturbance of intraocular tissue should be minimized, the timing of the second stage operation and the individualization of the operation design, and the medication and care after the operation should also be combined with the characteristics of children. Prevention of ocular trauma in children is the focus of attention. In the new information era, clinical research and public information platforms should be fully utilized to advance the epidemiological study of children's ocular trauma and guide the construction of the prevention and treatment system for children's ocular trauma more scientifically.

Uveitis is the most common extra-articular manifestation of juvenile idiopathic arthritis, typically as chronic anterior uveitis with insidious onset. Delayed and inadequate treatment may result in loss of patients' vision and even blindness. For refractory or severe uveitis related to juvenile idiopathic arthritis, systemic immunosuppressive agents should be used as early as possible. With the advantage of controlling ocular inflammation, avoiding ocular complications and reducing the use of traditional immunosuppressant drugs and glucocorticoid, tumor necrosis factor-α inhibitors have been new therapeutic options for uveitis associated with juvenile idiopathic arthritis, although methotrexate is known as the first-line approach. However, there are no internationally unified guidelines for clinical issues regarding the timing of application, reduction and withdrawal of tumor necrosis factor-α inhibitors, and no agreement on the application of tumor necrosis factor-α inhibitors in the management of ocular complications either. An in-depth understanding of the application status and progress of tumor necrosis factor alpha inhibitors in the treatment of juvenile idiopathic arthritis-associated uveitis has important clinical significance.

Kawasaki disease is a disease based on systemic vasculitis, which has become the most common disease in children with acquired heart disease, but its etiology and pathogenesis remain unclear.The homeostasis of the gut microbiota plays an important role in the maturation of the body′s immune system and immune regulation.The disorder of the gut microbiota is involved in a variety of inflammatory and immune diseases in children.Studies have found that there are changes in the composition of gut microbiota in children with Kawasaki disease.Gut microbiota disorders are related to the occurrence and development of Kawasaki disease, with the immune system function affected.Various mechanisms such as intestinal mucosal barrier dysfunction, immune inflammation, oxidative stress and metabolite effects promote the pathogenesis of Kawasaki disease.This article reviews the research on the relationship between gut microbiota and Kawasaki disease.

Animals ; Chiroptera ; virology ; Coronavirus ; classification ; genetics ; isolation & purification ; Coronavirus Infections ; transmission ; virology ; Databases, Genetic ; Genome, Viral ; Humans ; Male ; Middle Aged ; Middle East ; Phylogeny ; SARS Virus ; classification ; genetics ; isolation & purification ; Severe Acute Respiratory Syndrome ; virology

AIM: To establish a new quality standard for Kangbindu Oral Solution (Radix Isatidis, Gypsum Fibrosum, Rhizoma Phragmitis, Radix Rehmannia, etc.). METHODS: Rhizoma Acori Tatarinowii and Fructus Forsythiae were identified by TLC. The forsythin content was determined by HPLC on Inertsil ODS column (4.6mm?250mm,5?m). The mobile phase was acetomitrile water(25∶75). The detection wavelength was set at 277nm. RESULTS: The linear range of forsythin was in the range of 4.24～21.2?g. The average recovery was 99.09%, and RSD was 0.4%( n =6). CONCLUSION: The method is simple and accurate, and can be used for the quality control of Kangbindu Oral Solution.

Objective To develop a quality standard of Citrus limon oils.Methods Limonene in Citrus limon oils was identified by capillary gas chromatography and its content was determined by internal standard method.A polyethylene glycol elastic quartz capillary column (30 m of length,0.32 mm of internal diameter,0.25?m of film thickness) was used,column temperature being 90℃and split ratio being 5:1.Results In the range of 0.0627 mg/mL～4.0136 mg/mL for limonene,an equation was obtained:Y=1.53321X-0.05275,r=0.999 9.The average recovery of limonene was 97.2 % with RSD being 0.6 %.Conclusion The method is stable and reliable,and can provide evi- dences for the development of quality standard of Citrus limon oils.