Glycogen storage diseases (GSD) are a group of inherited disorders characterized by enzyme defects that affect the glycogen metabolism. Hypoglycemia, hepatomegaly, splenomegaly and growth retardation are the main clinical manifestations. The enzymes affecting glycogen synthesis and degradation are varied.Consequently, the clinical manifestations are different because of different enzyme defection. Most patients treated regularly can obtain the normal growth and grow to adults, but still can be accompanied long-term complications by adulthood. Now some scientists find new modified cornstarch is superior to standard therapy in maintaining blood glucose levels. Gene therapy is still in the experimental animals.

Triple negative breast cancer(TNBC) is defined by the lack of estrogen receptor (ER) , progesterone receptor (PR) and human epidermal growth factor receptor-2 ( Her-2) expression. It is a subtype of tumor with distinct clinical and pathologic features and lacks effective targeted therapies. It is non - sensitive to endocrine therapy and targeted therapy drug herceptin, leaving chemotherapy as the mainstay of treatment The feature and the treatment of TNBC is the hotspot of resent research.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Headache ; therapy ; Humans ; Male ; Pain, Intractable ; therapy ; Scalp ; Treatment Outcome

OBJECTIVETo explor the curative effect of double pedicle sliding skin flap for correction of cryptotia.

METHODSFrom January 2011 to October 2014, 7 cases with cryptotia (12 ears) were treated. We designed a double pedicle sliding skin flap above the helix. The flap was slided downward to form a new auriculocephalic sulcus. The other wound was covered by skin graft.

RESULTSAll wounds healed very well with no complication. The follow-up period ranged from 3 months to 1 year, with an average of (5.67 ± 3.38) months. The ear had stable appearance with no recurrence. The result was satisfactory.

CONCLUSIONSThe method is simple with reliable flap survival rates. The new auriculocephalic sulcus is stable and resistant. It's a good choice for correction of cryptotia.

Ear Auricle ; abnormalities ; surgery ; Ear Cartilage ; abnormalities ; surgery ; Follow-Up Studies ; Graft Survival ; Humans ; Reconstructive Surgical Procedures ; methods ; Surgical Flaps ; transplantation ; Time Factors ; Wound Healing

C-termini of proteins often play an important role in various biological processes, such as the transcription and translation from DNA to protein and also participating in various biological regulations. The determination of protein C-terminus is so crucial because it provides not only distinct functional annotation, but also a way to monitor the proteolysis-modified proteins. Based on the biological mass spectrometry, a series of novel methods and technologies were developed both for qualitative and quantitative analyses of protein C-terminus. These methods or technologies can be applied to accurate and effective protein C-terminus profiling, including the sequences and quantitative information of C-termini, which reveals the biological function of C-termini in life's activities and provides a better understanding of the degradation of mature proteins. Combined with our research, this review highlights the improvements in C-terminal proteomics study in the past decades, including the methodologies for recognition and identification of C-terminus, as well as the enrichment strategies for protein C-terminus.
Mass Spectrometry ; Protein Processing, Post-Translational ; Proteins ; chemistry ; Proteolysis ; Proteomics

Objective To investigate the factor that related to neoplasm from the clinicopathologic character of gallbladder polyps, and identify the reciprocal effectiveness of these factors. Method Three hundred and forty-seven patients who were underwent operation with gallbladder polypoid lesions were reviewed. Results Logistic regression analysis showed, only 5 parameters were significant for the prediction of neoplastic lesions, including age of the patient (P =0.015), the number of polyps (P=0.018), the maximal diameter (P=0.000), echo property (P=0.000), as well as the position of polyps (P=0.030),of which the maximal diameter was the best one to predict the neoplasm polyps. Further analysis with HOC curve showed that 0.925 cm, the diameter of polyps, was the critical point to calculate the polyp was neoplasm or not. Conclusions Age of the patient , the number of polyps , the maximaldiameter, the position of polyps, and echo property are independent factors in predicting gallbladder neoplastic lesions. The size of the polyps is the most significant one among the factors to predict gallbladder neoplasm with its critical point of 0.925 cm.

To investigate the correlation between traditional Chinese medicine (TCM) syndrome and short-term prognosis of ischemic stroke.

[Summary] _ Osteoporosis and diabetes mellitus are diseases which affect public heath severely, and osteoporotic fracture risk is increased in patients with diabetes. During recent years, new anti-diabetes drugs glucagon-like peptide-1(GLP-1) analogues and its receptor agonists had been used for diabetes treatment. As the research continues, besides of anti-diabetes, GLP-1 analogues are also found to play a role in bone metabolism and central nervous system by stimulating bone formation, inhibiting bone resorption, and nerve protection/nutrition, etc, suggesting that it may play an important role in the treatment of other diseases such as osteoporosis.

Objective To explore the causes of the special cases showing the EKG changes of cardiac infarction. Methods To analyse the causes of 30 special cases showing the EKG changes of cardiac infarction between 2000 and 2004. Results Among 30 special cases there were 11 cases of viral myocarditis( 36. 7 % ), 7 cases of acute pancreatitis(23.3% ) ,6 cases of hypertrophic obstructive cardiomyopathy(20% ) ,4 cases of bile-cyst and heart syn-drome(13. 3% ) and 2 cases of pulmonary embolism(6. 7% ). Conclusions There are many causes showing the EKG changes of cardiac infarction. It isn't the typical symptom of the cardiac infarction. It should be combined with the clinic and be distinguished timely and exactly in order to avoid the misdiagnosis.

Aim To establish the fatty liver animal model induced by high-fat diet.Observe the dynamic variety of the hepatic steatosis in the different time.Finding out the ideal animal model-making time of hyperlipidemia and nonalcoholic fatty liver.Methods 50 wistar rats were fed with high-fat diet.The serum TG,TC,HDL,LDL,AST,and ALT were detected from 2 to 6 weeks.At the same time,we analyzed the hepatic steatosis situation. Results The rats developed hyperlipidemia and slight fatty liver after two weeks.In the following weeks,the serum lipid level and liver index kept rising.So did the amount of steatosis cells in liver tissue.The 3～4 weeks animal model had developed moderate fatty liver and the 5～6 weeks animal model had developed serious fatty liver.Liver fibrosis was detected in the 6 weeks animal model.Conclusions Feeding with high-fat diet,different phases of fatty liver had been developed in six weeks,and could been used for correlative pharmacological test.