OBJECTIVE:To prepare gatifloxacin chitosan eye drops and to establish its quality control.METHODS:The eye drops was prepared with chitosan as the base.The content of gatifloxacin was determined by UV spectrophotometry and the stability of the preparation was detected by initial average accelerated method.RESULTS:The linear range for gati?floxacin was5.0～15.0?g/ml(r=0.9990,n=5),the average recovery rate was98.91%(RSD=1.43%).The activation energy for pyrolytic reaction of gatifloxacin chitosan eye drops was25.91kcal/mol,its shelflife at25℃and10℃were93.8days and950days,respectively.CONCLUSION:The preparation technology is feasible and the quality control method is reliable.

BACKGROUND: Currently the hematopoietic stem cells can be obtained from bone marrow, peripheral blood and cord blood, so it is expected to search a new source of stem cells in order to satisfy the clinical transplantation needs. From the 5th week of pregnancy, the blood sinusoid system develops completely in liver, and then hematopoietic stem cells can move with blood flow. OBJECTIVE: To observe the biological features of human fetal blood hematopoietic stem/progenitor cells (HS/PCs), and their transplantation into non-obese diabetic/severe combined immunodeficiency disease (NOD/ SCID) mice. DESIGN: Control trial. SETTING: Department of Hematology, First Affiliated Hospital of Guangxi Medical University. MATERIALS:①Cell resource: Twenty-one fetal blood samples were from dead fetus [gestational age of 18-29 weeks, mean (24.2±3.2) weeks] and twenty-one full-term cord blood samples were provided from the Department of Obstetrics, First Affiliated Hospital of Guangxi Medical University between October 2002 and February 2003, with the consent of their relatives.②Experimental animal: Twelve NOD/SCID female mice of 6-7 weeks old were bred in sterility and super-clean operation board. METHODS: Flow cytometer was used to assess cell surface markers of HS/PCs including CD34, CD38, HLA-DR and CD90 in 21 human fetal blood samples, and their expressions were compared with 21 human cord blood samples. Moreover, human fetal blood mononuclear cells (MNCs) were transplanted into 6 NOD/SCID mice irradiated sublethally. After 5 weeks, human leukocytic content was also detected in bone marrow of mice with flow cytometer while human Cart-1 gene in recipients' bone marrow was sensed with polymerase chain reaction (PCR).MAIN OUTCOME MEASURES: ① Expressions of HS/PCs surface markers in fetal blood and cord blood. ②Implantation of fetal blood cells into NOD/SCID mice.RESULTS: ①The percentage of CD34+ cells in fetal blood was significantly higher than that of full-term cord blood [(2.258 8±0.720 9)%,(1.572 9±0.478 3)%, P=0.000 4]. The percentages of CD34+CD38- cells and CD34+CD90+ cells in fetal blood were also higher than those of fullterm cord blood [(1.298 6±0.470 6)%, (0.871 0±0.409 5)%, P=0.001 6;(0.930 0±0.469 2)%, (0.560 0±0.365 8)%, P=0.032 4].②Four cases (4/6)of human fetal blood MNCs smoothly transplanted the hematopoiesis of sublethally irradiated NOD/SCID mice. Five weeks after the transplantation, human leukocyte and Cart-1 gene could still be detected in marrow cells of NOD/SCID mice.CONCLUSION: Human fetal blood contains more HS/PCs than cord blood. Human fetal blood MNCs can engraft bone marrow of NOD/SCID mice and reconstitute general hemopoiesis of marrow and lymph systems.Human fetal blood is a new possible source of pluripotential hematopoietic stem cell.

Breast Neoplasms ; genetics ; metabolism ; Carcinoma, Squamous Cell ; genetics ; metabolism ; Colorectal Neoplasms ; genetics ; metabolism ; Endometrial Neoplasms ; genetics ; metabolism ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Head and Neck Neoplasms ; genetics ; metabolism ; Humans ; Insulin-Like Growth Factor Binding Protein 1 ; genetics ; metabolism ; Insulin-Like Growth Factor Binding Protein 3 ; genetics ; metabolism ; Insulin-Like Growth Factor Binding Protein 5 ; genetics ; metabolism ; Insulin-Like Growth Factor Binding Proteins ; genetics ; metabolism ; Lung Neoplasms ; genetics ; metabolism ; Male ; Ovarian Neoplasms ; genetics ; metabolism ; Polymorphism, Genetic ; Promoter Regions, Genetic ; Prostatic Neoplasms ; genetics ; metabolism

Objective To observe the serum lipid level of patients with branch retinal vein occlusion (BRVO).Methods A total of 71 BRVO patients (BRVO group) were enrolled in this study.The patients included 31 males and 40 females,with an average age of (52.75 ± 10.2) years.All the patients were examined for visual acuity,slit lamp ophthalmoscopy combine with preset lens,fundus color photography and fundus fluorescein angiography (FFA) examination.Seventy-two age-and sex-matched normal subjects were enrolled in this study as control group.The subjects included 32 males and 40 females,with an average age of (53.10±9.5) years.The BRVO and control group were divided into four subgroup which including age with ＜40 years,40-49 years,50-59 years and ≥60 years.The plasma cholesterol and triglyceride level of BRVO group,control group,and age subgroups of BRVO and control group were comparatively analyzed.Results The average plasma cholesterol levels were (4.529±0.100) and (4.274±0.106) mmol/L in BRVO and control group,respectively.There was no difference between two groups (t=-1.738,P＞0.05).The average triglyceride levels were (1.500±0.129) and (1.319±0.095) mmol/L in BRVO and control group,respectively.There was no difference between two groups (t=-1.135,P＞0.05).There was no difference of average plasma cholesterol (t=-1.755,1.850,-1.892,-0.507) and triglyceride (t=0.846,-0.074,-1.288,-1.887) level in age subgroups of BRVO and control subgroup (P＞0.05).Conclusion There is no significant difference of serum lipid level between BRVO patients and controls.

Objective To investigate the value of MR multiparameter imaging for the clinical classification of chronic pancreatitis. Methods 65 patients with confirmed chronic pancreatitis by follow-up and pathologic examinations ( 14 mild, 37 moderate and 14 severe according to MANNHEIM system) and 20healthy volunteers were included in this study. MR examination including routine T1WI, T2WI, MRCP and dynamic enhanced MRI. The data were measured and statistical analysis was applied in four groups. Two radiologists assessed pancreatic duct diameter, pancreatic size, pancreatic cyst, pancreatic stone and pancreatic signal intensity on MRCP, T1-weighted and T2-weighted images. Pancreatic signal intensity were also measured on dynamic enhanced MR. Results Mean values of pancreatic signal intensity ratio on T1WI (rT1)in the pancreas were significantly reduced in patients with moderate and severe CP compared with volunteers.There was significant difference among four groups ( normal, 0. 98 ±0.27; mild, 0. 84 ±0. 12; moderate,0.81 ±0. 16; severe, 0.75 ±0.24). Mean values of pancreatic signal intensity ratio on T2WI (rT2) in the pancreas were no difference among four groups ( normal, 1.28 ± 0.3; mild, 1. 46 ± 0. 44, moderate, 1.46 ±0.55; severe, 1.76 ± 0.72). Pancreatic duct diameters were significantly increased in mild, moderate and severe CP groups [ mild (5.3 ± 2.4) mm; moderate ( 6.5 ± 3.3 ) mm; severe ( 8.1 ± 4.1 ) mm ] compared with patients without CP[ (2.0 ±0.6) mm; P < 0.01 ]. Severe degree of Cambridge classification was graded as mild in 4 (29%), moderate in 33 (89%), severe in 13 (93%). Pancreatic calcification was graded as mild in 2 ( 14% ), moderate in 11 (30%), severe in 5 (36%). Pancreatic pseudocyst was graded as mild in 0, moderate in 6 ( 16% ), severe in 3 (21.43%). Pancreatic parenchymal atrophy was graded as mild in 4 (29%), moderate in 22 (59%), severe in 10 (71%). They did not vary among CP groups. Parenchymal/arterial phase enhanced ratio (P/A) in the pancreas were significantly increased in patients with mild,moderate and severe CP (mild, 1. 10 ±0.08; moderate, 1.37 ±0.15; severe, 1.48 ±0.53) compared with patients without CP (0.88 ± 0.08, P < 0. 05 ). Significant correlation was present between the severity level of CP and the change of rT1, severe degree of Cambridge classification, the pancreatic duct diameter and P/A (r=0. 34, 0.41, 0. 62, - 0. 43; P < 0. 01 ). ROC analysis showed the presence of pancreatic duct diameters more than 2.5mm, rT1 less than 0. 8 and P/A more than 0. 8 had a sensitivity and specificity of diagnosing chronic pancreatitis of 94% and 79%, 90% and 48%, 95% and 47% respectively. Combined with the three variables, the specificity of diagnosing chronic pancreatitis can be improved to 95%.Conclusions T1-weighted, MRCP and dynamic enhanced MRI imaging can accurately evaluate the clinical severity of chronic pancreatitis. MRCP had the highest sensitivity and specificity, followed by T1-weighted and dynamic enhanced MRI imaging.

The study was conducted in vitro with human breast cancer cells BCaP-37, to determine the effects of selenium, vitamin A, vitamin E and a combination of these three nutrients on cell proliferation and cellular nucleic acid content. Selenium as sodium selenite had two phases of effect on cancer cell proliferation: the low concentrations of selenium (less than 5 ?M) stimulated cell growth and increased the cellular nucleic acid content; the high concentrations (more than 5 ?M) depressed cell growth and reduced the cellular nucleic acid content with dose-dependence. Vitamin A acetate inhibited cancer cell growth significantly, but vitamin A acid inhibited to some extent, and was less effective than vitamin A acetate. Vitamin E had less inhibitory effect compared to vitamin A acetate and the inhibitory percentages were lower than 40% in all treatment groups. Combination of selenium (5 ?M) and vitamin E (20mg/L) or selenium and vitamin A acetate (2mg/L), no synergism for the reduction of the contents of cellular nucleic acids (DNA and RNA) were observed. The combination of selenium, vitamin A acetate and vitamin E at such levels reduced cellular DNA and RNA contents obviously; RNA content was significantly lower than any other treatment group and was reduced synergis-tically. It was indicated that the combination of selenium, vitamin A acetate, vitamin E was synergistic for inhibition of cell proliferation. Results also showed the reversible tendency in the inhibition of cell proliferation by combination of these three nutrients. It was suggested that combination of selenium, vitamin A and E might be benificial for the prevention and adjuvant treatment of human breast cancer.

[Objective]To observe the cerebral activating effects of needling in Yanglingquan(GB34) vs a sham point of healthy volunteers based on the technique of fMRI.[Methods]Sixteen healthy volunteers were randomly divided into GB34 group and sham point group,and received needling in the different points on the right side respectfully.Super nuclear magnetic resonance(1.5T,GE Co.) was used to scan the brain and the obtained experimental data of the regional brain were processed and analyzed by method of Region of Interest(ROI).[Results]There was not any difference between needling in GB34 and the sham point in the cerebral activating rate.While the activating number of needling in the sham point was more than that of needling in GB34 in bilateral parietal lobes and right occipital lobe(P

Objective To investigate the change and its clinical significance of serum erythropoietin(EPO) content in the patients with acute cerebral stroke.Methods The serum EPO content in 60 patients with acute cerebral infarction(ACI),45 patients with cerebral hemorrhage(CH) and 40 patients with other nervous system diseases(control group) were detected by radio-immunity means.The patients with acute cerebral stroke were evaluated by National Institute of Health Stroke Scale(NIHSS).Results The serum EPO content in ACI group and CH group [(1.64?0.41)ng/ml,(1.59?0.54)ng/ml] were significant higher than that in the control group [(1.17?0.86)ng/ml](all P

Objective To investigate the effect of acupuncture at the primary points of the three yin meridians of the foot on blood pressure in an animal model of high/low blood pressure and explore the specific effect of the meridian points-the primary points of the three yin meridians of the foot.Methods The study included the parts of hypertension and hypotenstion. Both parts were allocated to the primary points of the three yin meridians of the foot, non-acupoint, model and blank groups, six rats each. Except the model and blank groups, all the groups were given acupuncture treatment for seven consecutive days. Blood pressure was measured after the completion of treatment. Five consecutive measurements were made in each rat. Mean values of blood pressure were taken.Results For the part of hypertension, blood pressure at each time point was higher in the acupuncture groups than in the blank control group and lower than in the model group, and there were statistically significant differences (P<0.05), indicating that acupuncture at the primary points of the three yin meridians of the foot has a hypotensive effect. The hypotensive effects of Taichong and Taixi were better than those of Taibai and the non-acupoint (P<0.05). The hypotensive effects of Taichong and Taixi were equal (P>0.05). The hypotensive effect of Taibai was equal to that of the non-acupoint (P>0.05). For the part of hypotension, blood pressure at each time point was lower in the acupuncture groups than in the blank control group and higher than in the model group, and there were statistically significant differences (P<0.05), indicating that acupuncture at the primary points of the three yin meridians of the foot has a pressor effect. The pressor effect was worse in the the non-acupoint group than in the primary points of the three yin meridians of the foot group (P<0.05). The pressor effects of Taibai and Taixi were equal but worse than that of Taichong (P<0.05).Conclusions Acupuncture at the primary points of the three yin meridians of the foot has a specific bidirectional regulating effect on blood pressure. Of them, point Taichong produces a best bidirectional regulating effect on blood pressure.

0.05 ), while the age when the weight gain reached 2 000 g was (41?27) days, there was significant differences among the groups ( F=4.52,P