1.Application of Multi-slice Spiral Computed Tomographic Scan in Rhinology
Jun CAI ; Junfeng JI ; Guangming LU
Chinese Medical Equipment Journal 1993;0(06):-
Objective To evaluate the value of multi-slice spiral CT in nasal endoscopic surgery.Methods The multi-slice spiral CT data of 232 patients who were scanned prior to nasal endosopic surgery were analyzed.Results The nasal and sinus disease could be diagnosed correctly with multi-slice spiral CT.The ostiomeatal complex and the channels of the frontal sinus' drainage could be showed clearly.All the manifestation of the multi-slice spiral CT was in correspondence with the endoscopy showed in the surgery.Conclusion The multi-slice CT has the advantages of short scan time,less radiation dosage and multiplanar reconstruction.So it can display the nasal and sinus structures in detail,and has a great value in guiding the nasal endoscopic surgery.
2.Drug Resistance of Pathogens in Pediatric Intensive Care Unit
Junfeng KONG ; Bing WANG ; Lu LEI
Chinese Journal of Nosocomiology 2005;0(11):-
OBJECTIVE To investigate the drug resistance of pathogens in pediatric ICU and discuss how to treat infections caused by these resistant strains.METHODS After genus identification,bacterial susceptibility test was carried out using Kirby-Bauer disk method.RESULTS A total of 270 clinical isolates were analyzed including 194(72%) strains of Gram-negative bacilli and 76(28%) strains of Gram-positive cocci.From them 182(67%) strains were isolated from sputum,78(29%) strains from blood and 10(4%) strains from secretion.The most common bacilli were Klebsiella spp,coagulase negative Staphylococcus,Escherichia coli,Enterococcus spp and Pseudomonas aeruginosa.About 66.7% of E.coli and 91.1% of Klebsiella spp isolates produced ESBLs,the isolating rate of MRCNS was 83.9%.CONCLUSIONS The characteristics of pathogen distribution and drug resistance surveillance must be understood in order to use antibiotics rationally,to control the increasing trend of drug resistance pathogens and to forbid the break out of nosocomial infections.
4.Effect of Yangyin Kangdu Powder on Peripheral Blood T-lymphocyte Subsets in X-ray-irradiated Mice
Yuan LU ; Dacan CHEN ; Junfeng HOU ; Guowei XUAN
Journal of Guangzhou University of Traditional Chinese Medicine 2004;0(05):-
[Objective] To investigate the effects of Yangyin Kangdu Powder (YKP) on peripheral blood T-lymphocyte subsets levels in X-ray-irradiated mice. [Methods] Forty-five mice were randomized into normal control, model and YKP groups. Except the normal group, the rats in model and YKP groups were irradiated with 6Gy X-ray to establish models with acute radiation-induced injury. Normal saline was given to the model group while YKP in the dosage of 10 g?kg-1?d-1 was orally administered to YKP group for one week. The changes of CD4 and CD8 T-lymphocyte subsets levels were detected by flow cytometry. [Results] Plasma levels of CD4 and CD8 and the ratio of CD4/CD8 decreased in the model group, the difference being significant as compared with the normal control group (P
5.Antiproliferation effects of oridonin on hepatocellular carcinoma BEL-7402 cells and its mechanism
Junfeng ZHANG ; Guihua CHEN ; Minqiang LU ; Jiajun LIU
Chinese Traditional Patent Medicine 1992;0(09):-
AIM: To investigate the antiproliferation effects of oridonin on hepatocellular carcinoma BEL-7402 cells and its mechanisms of action. METHODS: BEL-7402 cells in culture medium were treated with different concentrations of oridonin. The inhibitory rate of the cells was measured by MTT assay. Cell apoptotic rate was detected by flow cytometry (FCM). Morphology of cell apoptosis was observed by Hoechst 33258 stain. Reverse transcriptase polymerase chain reaction (RT-PCR) and PCR-enzyme-linked immunosorbent assay (ELISA) were used to detect hTERT mRNA expression and telomerase activity before and after apoptosis. RESULTS: Oridonin could inhibit the growth of BEL-7402 cells and cause apoptosis significantly. The suppression was in both time-dependent and dose-dependent manner. Marked morphological changes in cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by Hoechst 33258 stain especially after the cells were treated 48-60 h by oridonin. The expression of hTERT mRNA as well as activity of telomerase decreased concurrently by treatment with oridonin in BEL-7402 cells. CONCLUSION: Oridonin has apparent antiproliferation and apoptosis-inducing effects on BEL-7402 cells in vitro, downregulation of the hTERT mRNA expression and decreasing the telomerase activity of BEL-7402 cells may be one of its important anti-hepatocellular carcinoma mechanisms.
6.Etiology of laryngeal stridor in 297 cases of infants
Jun LI ; Jin WANG ; Zhenjun LU ; Junfeng LI
Chinese Pediatric Emergency Medicine 2012;19(3):248-249
ObjectiveTo summarize the cause of laryngeal stridor in infants in order to make accurate diagnosis and treatment of the disease.MethodsWe reviewed medical records of 297 cases of patients less than 3 year of age with the presenting symptom of stridor who were initially evaluated in the outpatient setting of otorhinolaryngological department from Jan 2005 to Jan 2010.The causes of stridor were clarified by examinations of ultrafine electronic laryngoscope,throat three-dimensional CT,and bronchoscopy in all cases.Patients underwent history-taking,physical examination and flexible laryngoscopy,CT examination or bronchoscopy evaluation in the operating room.ResultsOf all 297 patients,199 cases ( 67.0% ) were diagnosed as congenital airway abnomalities for cause of stridor,which included congenital laryngeal abnomalities in 169(84.9%,169/199) and congenital tracheal abnormalities in 30 cases( 15.1%,30/199).Another 98 cases (33.0%,98/297) were diagnosed as acquired disease for cause of stridor.The most congenital laryngeal anomaly was laryngomalacia ( 159,94.1%,159/169 ).The most congenital tracheal abnormalities was tracheomalacia ( 14,46.7%,14/30 ).Sixty-four cases ( 65.3%,64/98 ) were diagnosed as foreign body in airway and 26 cases (26.5%,26/98) were respiratory infection,which were the first and second most common causes of acquired disease for stuidor.ConclusionCongenital airway structural abnormalities as a major cause of infant laryngeal stridor,followed by acquired disorders,including airway foreign body and infection.
7.Progress of clinical application of functional MRI in the localization of brain language area.
Nan ZHANG ; Junfeng LU ; Jinsong WU
Chinese Journal of Surgery 2016;54(2):157-160
For surgical operation in the functional area in the brain, it's commonly demanded to resect the lesion to the maximal extent on the basis of preserve the normal neural function, thus the precise localization of functional area is extremely important. As for the advantages of being widely available, easy to grasp and non-invasive, the functional MRI (fMRI) has come into wide use, while the application of language fMRI is still in the initial stage. It's important to choose appropriate fMRI task according to the individual condition of the subject, the commonly-adopted tasks include verb generation, picture naming, word recognition, word generation, etc. However, the effectiveness of using fMRI to localize language area is not totally satisfactory, adopting multiple task is an effective approach to improve the sensitivity of this technique. The application of resting state fMRI in the localization of language area and the further research of the role of fMRI in localizing the Chinese language area are the important future directions.
Brain
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physiology
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Brain Mapping
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Humans
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Language
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Magnetic Resonance Imaging
8.Epidemiological analysis on the first outbreak of tsutsugamushi disease in Fuyang City of Anhui Province in 2008
Jiabing WU ; Dailin HU ; Fan HE ; Meijuan LU ; Junfeng WAN ; Liye ZHU
Chinese Journal of Disease Control & Prevention 2008;0(06):-
Objective To analyze the epidemiological and clinical characteristics of an outbreak of tsutsugamushi disease in Fuyang City of Anhui Province.Methods Epidemiological information was collected by case investigation questionnaire of tsutsugamushi disease.Diagnosis was determined by the detection of OXK antigen by Weil-Felix test.Results Seventy-eight cases with tsutsugamushi disease were discovered in the outbreak.All the patients had an experience of working in this area before the starting of symptoms.The clinical features of the patients were fever,headache,skin eschar,tetter and lymphadenectasis.No severe complications occurred in each patient.All the patients recovered after getting deoxycycline.Conclusions This was the first report for the tsutsugamushi disease occurred in Fuyang City of Anhui Province.And the declaration for the new happened tsutsugamushi disease would provide strong support evidence for the prevention and control of this event and ensure the safety for working in this area.Also the deoxycycline was very effective for the treatment of tsutsugamushi disease.
9.The changes on expression of IL-1βand IL-13 mRNA in lung tissue and serum after drowning rats
Meiling JIANG ; Wenping GUO ; Jinfang ZHAO ; Junfeng LU ; Ze MEI ; Jiajia LIU ; Xinhua LIANG
Chinese Journal of Forensic Medicine 2017;32(2):137-140
Objective To observe the change in IL-1β,IL-13mRNA expression in drowning rat lungs and serum,so as to investigate the significance of IL-1β and IL-13 mechanism in the development of drowning.Methods SD rats were randomly divided into control group,drowning group.Then using TaqMan probe method to determine the expression of IL-1β and IL-13 mRNA in Right lower lobe of lung tissue and the serum of right ventricle,which were extracted respectively from each group of rats.Results (1) The lung tissue morphological changes:Typical appearance signs and anatomy of drowning group meet ante-mortem drowning feature.(2) The expression of IL-1β,IL-13 in lung tissue:compared with the control group,the expression of IL-1β and IL-13 were slightly decreased,which has no statistical significance.(3) The expression of IL-1β and IL-13 in serum:compared with the control group,the expression of IL-1β and IL-13 were significant increased,both of which has statistical significance.Conclusion (1)The expression of IL-1β and IL-13 were decreased in lung tissue may be due to drowned rats present compensatory anti-inflammatory response syndrome which causes immune incompetent performance.(2) The expression of IL-1β and IL-13 were significant increased in serum may be relate to drown stress and drowning associated acute lung injury after traumatic stress.
10.Study of dose variations induced by MLC weight effect for IMRT delivery
Xiaoguang LU ; Junfeng WANG ; Fei LIU ; Dingyu LI ; Qi MEI ; Guangyuan HU
Chinese Medical Equipment Journal 2017;38(3):90-93
Objective To evaluate dose variations induced by gravity of multi-leaf collimator to provide references for clinical intensity-modulated radiotherapy.Methods Two-dimensional dose distributions in the central plane of IMRT fields were measured by use of a 2D ion chamber array.All measurements were repeated at two collimator angles (C=0 and 90°),for each of the following gantry angles:G=0 and 270°.Comparisons were made to dose distributions generated at G=0°and their differences were analyzed using gamma index analysis (3%/3 mm and 1%/1 mm).Results Under the radiation field of 10 cm×10 cm,the gamma passing rate was higher than 99% for 3% 3 mm anch close to 95% for 1%/1mm Under a 3%/3mm error standard,the average matching rate for step & shoot fields was (96.46±0.33)% and for DMLC fields was (94.67±0.54)% at C=0°;The average matching rate for step & shoot fields was (94.59±0.47)% and for DMLC fields was (92.60±0.52)% at C=90°.Under a 1%/1mm error standard,the average matching rate for step & shoot fields was (89.83 ±1.06)% and for DMLC fields was (85.84±0.57)% at C=0°;The average matching rate for step & shoot fields was (86.91 ±1.71)% and for DMLC fields was (83.89±0.69)% at C=90°.Concusion MLC weight effect affects IMRT delivery dose,and DMLC fields are more sensitive to gravity than step & shoot fields.