Objective To study the effects of mifepristone on the expression of estrogen receptor(ER)? and progesterone receptor(PR) in the endometrium of patients with adenomyosis in different menstrual cycles. Methods Forty-seven patients with adenomyosis and 15 normal subjects were divided into 3 groups: mifepristone group(n=24),non-drug group(n=23) and control group(n=15).The expression of ER? and PR in eutopic,ectopic and normal endometrium in proliferative phase and secretory phase were detected by immunohistochemistry. Results In non-drug group,the expression of ER? and PR in ectopic endometrial glandular and stromal cells was significantly lower than that in eutopic and normal endometrium(P0.05).The expression of ER? and PR in both ectopic and eutopic endometrial glandular and stromal cells in mifepristone group was lower than that in the non-drug group(P

The color fading reation on potassium bromate with Victoria green stand G by the catalysis of nitrite in hydrochloric acid medium was studied. The experimental condition offlow injection spectrophotometric method for the determination of trace NO2- was optimized. The linear range for the determination of nitrite is 0.00～0.30 mg/L and 0.30～ 2.00 mg/L, the solpes of standard curves are 0.708 and 0.339 respectively, the analytical speed is 80/h. It has been used todetermine trace nitrite in the collanae lake water, fishpond water, power plant waste water and well water. The results are satifactory.

Nuclear factor-E2-related factor 2(Nrf2)is a member of C′n′C transcription factor family.It is an important transcrip-tion factor for regulation of cellular redox status and can be seen in all kinds of tissues .Recent studies have demonstrated that rapid deg-radation of Nrf2 after gene-induced antioxidative stress is as important as transcription and activation of Nrf 2 and the nuclear export of Nrf2 is a prerequisite for rapid degradation of Nrf2 in the cytosol.This review focuses on the mechanism of nuclear export of Nrf 2.

Objective To analyze the direct interaction between mmu-miR-30b and mouse FoxO3 (mFoxO3) mRNA.Methods Three target gene fragments, which were respectively 402, 123 and 299 bp in length, were amplified from mouse cDNA by PCR using specific primers and site-direct mutant primers.A complete mutant fragment was obtained by joining together the 123 and 299 bp fragments.Recombinant plasmids, pmirGLO-mFoxO3 and pmirGLO-mFoxO3 mt, were constructed through inserting wild and mutant fragments of mFoxO3 into pmirGLO vector, respectively.HEK 293T cells were respectively co-transfected with the constructed recombinant plasmids and mmu-miR-30b/mmu-miR-30b inhibitor.Dual-luciferase reporter assay system was used to determine the Firefly-Renilla luciferase activity in different groups.Western blot assay was performed to evaluate the regulatory effect of mmu-miR-30b on mFoxO3 expression.ResultsRestriction enzyme analysis and gene sequencing showed that the two recombinant plasmids, pmirGLO-mFoxO3 and pmirGLO-mFoxO3 mt, were constructed successfully.The activity of Firefly-Renilla luciferase in HEK 293T cells transfected with pmirGLO, pmirGLO+mmu-miR-30b, pmirGLO+mmu-miR-30b inhibitor, pmirGLO-mFoxO3+mmu-miR-30b, pmirGLO-mFoxO3+mmu-miR-30b+mmu-miR-30b inhibitor, or pmirGLO-mFoxO3 mt+mmu-miR-30b was 13.18±0.97, 14.35±0.99, 12.46±1.20, 9.55±1.11, 13.71±0.89 and 10.99±0.92, respectively.Compared with pmirGLO+mmu-miR-30b group, the luciferase activity was significantly decreased in pmirGLO-mFoxO3+mmu-miR-30b group (P<0.05), but showed no significant change in pmirGLO-mFoxO3 mt+mmu-miR-30b group (P>0.05).In addition, the suppressed Firefly-Renilla luciferase activity in pmirGLO-mFoxO3+mmu-miR-30b group was restored by mmu-miR-30b inhibitor treatment (P<0.05).Enhancing the expression of mmu-miR-30b could markedly inhibit the expression of mFoxO3 at protien level (P<0.05), and that could be significantly attenuated by mmu-miR-30b inhibitor treatmeat (P<0.05).Conclusion mFoxO3 mRNA is a novel target gene of mmu-miR-30b.There is a direct interaction between mmu-miR-30b and mFoxO3 mRNA.

Objective To consecutively investigate the changes of the superior vena cava flow Doppler patterns in patients with superior vena cava syndrome (SVCS) and predict SVCS development.Methods Twenty-two definative cases with SVCS due to tumor were enrolled in this study.At different stages of therapy the superior vena cava was detected by ultrasonography.The superior vena cava Doppler spectra during cardiac cycle and respiratory cycle were analyzed respectively.Results In 22 patients with SVCS the superior vena cava Doppler flow patterns presented a developing tendency,from turbulent,continuous and high velocity pattern to normalization.The S and D waves peak velocities were ( 154.78 ?52.15 ) cm/s,( 159.38 ? 46.56 ) cm/s respectively before therapy,while after therapy ( 58.78 ? 33.43 ) cm/s,( 34.96 ? 17.56 ) cm/s respectively.The S wave and D wave respective differences in respiratory cycle were ( 2.14 ? 2.08 ) cm/s,(2.73?2.68)cm/s before therapy,while those were ( 7.68 ? 6.22 ) cm/s,( 6.32 ? 4.98 ) cm/s after therapy.Conclusions The regular changes of the superior vena cava Doppler flow patterns in tumor-induced SVCS development course were embodied in dynamic diversity.These changes could suggest SVCS development and evaluate the therapy effect on SVCS.

Gut microbiota plays an important role in the maintenance of physiological function and genesis of some gut diseases. Brain-gut axis,as an important link between brain and gastrointestinal tract,is a requisite of gut microbiota stability. The dysfunction of brain-gut axis may lead to intestinal dysbiosis through activation of intestinal immune activity. Conversely,intestinal dysbiosis can influence nervous system development and may cause dysfunction of brain-gut axis,in which vagus nerve and metabolites in serum may be the critical factors. This article reviewed the advances in study on interaction between gut microbiota and brain-gut axis.

[Summary] Limb numbness is a common symptom of diabetic peripheral neuropathy(DPN),which is similar to lumbar vertebra disease and spinal cord tumor compression syndrome. Diabetes mellitus (DM) combined with lumbar spondylosis and spinal cord tumor compression is rarely occurred and is difficult to distinguish from DPN . Here we reported a case of DM combined with spinal neurilemoma that was misdiagnosed as DPN for half a year.

Objective To analyze the flow spectra of in ternal jugular vein (IJV) in healthy adults by pulsed Doppler ultrasonography. Methods IJV flow velocities in 120 healthy volunteers were recorded during cardiac and respiratory cycles respectively with the transducer placed in cervical region view. Results In healthy subjects, IJV waveforms were composed of one reverse wave A and two forward waves S and D during cardiac cycle. The flow velocities of wave S [( 27.17 ? 19.88 )cm/s vs ( 19.04 ? 14.26 )cm/s] and wave D [( 17.43 ? 12.78 )cm/s vs ( 12.70 ? 10.57 )cm/s] were quicker during inspiration than those during expiration (P 0.05 ]. The velocities of wave S and wave D in the group aged over sixty years old were lower than those in the group aged between 20-60 years old(P

Objective To evaluate the expression of endothelial Per-ARNT-Sim domain protein-1(EPAS-1)/hypoxia-inducible factor-2?(HIF-2?) and its relationship with vascular endothelial growth factor (VEGF) in bladder transitional cell carcinoma (BTCC). Methods The expression of EPAS-1/HIF-2? and VEGF by immunohistochemical staining was examined in 60 cases of BTCC (Grade Ⅰ in 28 cases,Grade Ⅱ in 12,Grade Ⅲ in 20) and 8 subjects with normal urothelium. Of the 60 cases 29 had superficial (≤pT 1) bladder cancer and 31 had invasive (≥pT 2 bladder cancer).?2 test was used to assess the relationship between EPAS-1/HIF-2? and VEGF expression versus tumor grade and stage. Results The expressions of EPAS-1/HIF-2? and VEGF were negative in all normal bladder tissue but high in BTCC.Of the 60 cases,34 (56.6%) were positive and 26 ( 43.4%) were negative for EPAS1/HIF-2?.Four cases (14.3%) of Grade Ⅰ,11 (91.7%) of Grade Ⅱ and 19 (95.0%) of Grade Ⅲ were positive for EPAS-1/HIF-2?. Positive staining was seen in 5 (17.2%) of the superficial and 29 (93.5%) of the invasive cancer cases.EPAS-1/HIF-2? positivity was correlated with high histological grade (r=0.862,P

Objective In order to further understand the bioactivity of sphingosine kinase, high efficient replication defective recombinant adenovirus carrying the wild type SPK gene (SPK WT ) and SPK mutant gene (SPK DN ) were constructed respectively. Methods The SPK genes of interest were subcloned into a shuttle vector pshuttle cmv respectively. Pshuttle cmv SPK WT and Pshuttle cmv SPK DN were linearized by PmeI and transformed respectively into the competent cells of E. coli BJ AD 1 cells which contain adenoviral backbone plasmid pAdEasy 1. The linearized recombinant plasmids were transfected into adenovirus 293 packaging cells respectively,and then recombinant adenoviruses were harvested. We performed the PCR, Western blot and enzyme assays to identify the recombinant adenoviruses. Results The recombinant adenoviruses containing the interest gene and being able to infect ECV 304 cells were obtained. Overexpression of wild type SPK gene in ECV 304 cells increased the endogenous SPK activity, whereas overexpression of mutant SPK (SPK DN ) inhibited intracellular SPK activity. Conclusion Recombinant adenoviral vectors can mediate interesting gene expression in cells.