Objective To explore the effect of methylprednisolone combined with high dose immunoglobulin (IVIg) in treatment of severe bullous skin disease. Methods 51 cases of severe bullous skin diseases were randomly divided into control group and observation group and control group received conventional methylprednisolone treatment on the basis of the observation group in this high-dose injection combined with IVIg,therapeutic effect were observed.Results The observation group hospitalization days ( 15.6 ± 6. 2) d, hormone usage ( 87. 6 ± 13.7 ) mg, hormone reduction time(8. 6 ±2. 4) d,were obviously lower than the control group(23. 1 ±6. 3)d,( 105.3 ± 1.64) mg, ( 13.8 ±4. 1 ) d ( t = 2. 771,3. 102,3. 219, P ＜ 0. 05 ). The observation group total effective rate was significantly higher than the control group( x2 =6. 248,P ＜0. 05). The observation group therapy effect-acting period and obviously subsidise lesions time was(8. 9 ±2. 4)d, (17.6 ±3. 7)d respectively,were significantly lower than the control group( 13. 1 ±3. 8) d, (23. 6 ± 5. 1 ) d, the differences between the two groups were statistically significant ( t = 2. 893,3. 347, P ＜0. 05). The observation group complication occurred in 5 cases were lower than those of the control group of 9 cases (x2 = 5.245 ,P ＜ 0. 05 ). Conclusion Methylprednisolone combined with high dose IVIg treatment of severe bullous skin disease could shorten the course and speed up the restoration of skin lesions, reduce and alleviate the glucocorticoid dosage and side effects, improve the cure rate , and it was worthy of promotion.

tep immunochromatographic method accounted for 96.4%.Conclusion Both RT-PCR and step immuno chromatographic method can not be used as means of short-term therapeutic effect evaluation.

This paper is aimed to evaluate the CT manifestation of congenital pectus excavatum and its effect on the cardiac morphology. CT features of 34 children with pectus excavatum were retrospectively reviewed. The Haller index (HI), CT depression index (CTDI), cardiac rotation angle (CRA), pulmonary vein angle (PVA), cardiac compression index (CCI) and cardiac asymmetry index (CAI) were measured from the images on the PACS system. The relationships among these indexes were evaluated. The HI was 4.12 +/- 1.48, the CTDI was 2.39+/- 0.70, CRA was (53.52 +/- 7.68) degrees, PVA was (66.51 +/- 11.02) degrees, CCI was 2.43 +/- 0.95, and CAI was 1.55 +/- 0.56. There was statistical significance of CCI and CAI between children with severe or slight pectus excavatum. The HI and CTDI were positively related with CCI and CAI respectively, whereas there was no statistical significance of CRA and PVA between children with severe or slight pectus excavatum. There was no statistical significance of HI, CTDI, CRA, PVA, CCI and CAI among different age groups. The morphological change of the heart compressed in children with pectus excavatum can be accurately evaluated by multislice helical CT.
Child ; Funnel Chest ; pathology ; Humans ; Myocardium ; pathology ; Retrospective Studies ; Tomography, Spiral Computed

Senile patients take a big part in the whole population of patients in endodontic department.This article analyzes psychological state of senile patients in endodontic department from the view of medical ethics and studies corresponding nursing countermeasures so as to find a good cooperation of doctors,nurses and patients and improve treatment effect.Suggested countermeasures are as follows.a.Improve the comprehensive quality of nursing staff.b.Respect and cherish senile patients,and meet their reasonable requests.c.Establish high moral responsibility and correct values of ethics and morality.d.Pay attention to the mutual communication between nurses and patients and psychological support.f.Recognize the importance of health education to senile patients before,in and after medical treatment.

OBJECTIVE To investigate probationer nurses' knowledge about nosocomial infection (NI) and clean operating-room. METHODS Survey among probationer nurses was performed by self-designed questionnaires. RESULTS 47% probationer nurses thought it very necessary to strength knowledge about NI before probation. The scores of clean operating-room knowledge was lower than that nosocomial infection (NI); The cognition rate among high educated probationer nurses were higher than low educated,P

Objective　To summarize the phenotypic and genotypic characteristics of mitochondrial combined oxidative phosphorylation deficiency type 1 (COXPD1), and to improve the clinicians' awareness of this mitochondrial encephalomyopathy. Methods　The clinical characteristics, physical examination, laboratory examination and other data of a child with COXPD1 were analyzed retrospectively. The diagnosis was confirmed by clinical whole exon sequencing and high-precision mitochondrial genome full-length PLUS gene detection, and the phenotype and genotype were analyzed by reviewing relevant literature. Results　A one-year and five-month-old boy mainly presented with hyperlactacidemia and abnormal liver function. Clinical whole exon sequencing showed that the child had homozygous variation of c. 688G>A(p.G230S) in the GFM1 gene. Sanger sequencing verified that the variation was respectively inherited from the parents of the child (both were heterozygous) with the autosomal recessive inheritance pattern. The high-precision mitochondrial genome full-length PLUS detection also did not find pathogenic mutations related to clinical phenotypes. The child was diagnosed with COXPD1. After "cocktail" therapy and liver protection therapy, the patient's condition improved. Conclusions　The phenotype of COXPD1 is complicated and variable, mainly liver type and brain type. The mutation of GFM1 gene affects mitochondrial translation system function, and early gene detection is helpful for definite diagnosis.

Objective: To study the chemical constituents of Stephania hernandifolia. Methods: Various chromatographic techniques were used to isolate the constituents, and the structures were elucidated on the basis of HR-EI-MS, 1D-NMR and 2D-NMR spectral analyses. Results: A hasubanan alkaloid, hernsubanine D (1) was isolated from the whole plants of S. hernandifolia. The compound was screened for the cytotoxic activity against two human cancer cell lines in vitro. Conclusion: Compound 1 is a new compound without cytotoxicity against A549 and K562 cells. © 2014 Tianjin Press of Chinese Herbal Medicines.

Objective To study the chemical constituents in the seeds of Euphorbia lathyris.MethodsCompounds were isolated by methods of column chromatography(silica gel,including reversed phase),Sephadex,and recrystallization.On the basis of spectroscopic methods including IR,MS,NMR,and X-ray,structures of compounds were confirmed.Results Twenty-two multi-type compounds were isolated from ethanol extract in the seeds of E.lathyris.Their structures were identified as 5,15-O-diacetyl-3-O-phenyl-6(17)-epoxylathyrol(1),5,15-O-diacetyl-3,7-O-dibenzoyl-7-hydroxylathyrol(2),5,15-O-diacetyl-3-O-benzoyl-lathyrol(3),20-O-hexadecanoyl-ingenol(4),3-O-hexadecanoyl-ingenol(5),15,17-O-diacetyl-3-O-cinnamoyl-17-hydroxyjolkinol(6),5,15,17-O-triacetyl-3-O-benzoyl-17-hydroxyisolathyrol(7),5,15-O-diacetyl-3-O-nicotinoyl-lathyrol(8),5,15-O-diacetyl-3-O-benzoyl-7-O-nicotinoyl-7-hydroxy-lathyrol(9),ingenol(10),lathyrol(11),esculetin(12),?-sitosterol(13),benzene-1,2,3-triol(14),palmiticacid(15),2,3-dihydroxypropyl icosanoate(16), 2,3-dihydroxypropyl oleate(17),2,3,4-trihydroxybutyl hexadec-3-enoate(18),aurantianide acetate(19),benzoic acid(20),p-hydroxybenzoic acid(21),oleic acid(22).Conclusion Among these,compounds 10,11,1419 are obtained from this plant for the first time and compounds 1-3 are the main diterpenes.

BACKGROUND:Researches on establishing restenosis models in rats emphasize the importancc of 2.0 Forgarty balloon catheter import,but little has been mentioned about domestic-made balloon catheter.OBJECTIVE:To explore the characteristics of vessel restenosis model by denuding arterial endothelium with domestic-made 2.0 Forgarty balloon catheter in rats.DESIGN:A randomized controlled animal experiment.SETTING:Laboratory of Pathophysiology,Hunan University of Traditional Chinese Medicine.MATERIALs:This study was performed at the Laboratory of Pathophysiology,Hunan University of Traditional Chinese Medicine between September 2006 and January 2007.Healthy male SD rats,weighing 300-350 g,were selected.The protocol was performed in accordance with ethical guidelines for the use and care of animals.Domestic-made 2.0 Forgarty balloon catheters were made of balloons and catheters.Proliferating cell nuclear antigen(PCNA),collagen I,and SM α-actin immunohistochemical staining kits were provided by Wuhan Boster Bioengineering Co.,Ltd,China.METHODS:The rats were randomly divided into 4 groups:sham-operated group(n=5),7th day model group(n=4),14th day model group(n=5),and 21st day model group(n=4).The vessel restenosis models were established in the latter 3 groups by denuding arterial endothelium with domestic-made balloon catheter in rats.In the sham-operated group,rats were without insertion of balloon catheter.Injured thoracic aortas were taken out for HE staining to observe histological changes of vascular intima.At the meantime,expressions of SM α-actin,PCNA and collagen I were determined by immunohistochemical method in each group.MAIN OUTCOME MEASURES:Histological changes of intima at the thoracic aorta as well as expressions of SM α-actin,PCNA and collagen I.RESULTS:Eighteen rats were included in the final analysis.The aortal vascular walls remained integrity without narrowness of the lumen areas and proliferation of the endothelium,vascular smooth muscle cells(VSMCs)ranged regularly in the mesolamella in the sham-operated group.The endothelium had little proliferation in the 7th day model group.The intima thickened and the area of lumen became narrow in the 14th day model group.The intimal hyperplasia was diffusive and progressive which mainly included VSMCs,the area of lumen narrowed markedly and cells ranged disorderly in mesolamella in the 21st day model group.In the 14th day model group,PCNA level was higher compared to the remaining 3 groups(P＜0.01).SM α-actin level were significantly higher in the 14th and 21st day model groups than in the sham-operated group and 7th day model group(both P＜0.01).There were no significant differences in collagen I among the groups(P＞0.05).CONCLUSION:Vascular intima hyperplasia appeared markedly 14-21 days after domestic-made balloon catheter-induced rat aorta injury.The results suggested that intima hyperplasia is mainly induced by proliferation of VSMCs.

BACKGROUND:Corneal neovascularization not only seriously affects vision, it is also a high-risk factor for the rejection after allogeneic ceratoplasty, thus it is always a hot issue and in ophthalmology to investigate the pathogenesis of corneal neovascularization and the inhibitors for blocking its formation.OBJECTIVE: To induce model of corneal neovascularization in rats using nylon suture, and investigate the mechanism of nuclear factor-кB (NF-кB) in the occurrence and development of corneal neovascularization using dexamethasone as the glucocorticoid inhibitor for corneal neovascularization.DESIGN: A randomized controlled trial.SETTING: Department of Ophthalmology, Zhujiang Hospital of Southern Medical University.MATERIALS: The experiments were carried out in the Zhujiang Hospital of Southern Medical University from January to April 2005. Fifty-five healthy male Wistar rats of clean degree were used. Rabbit-anti-rat NF-кB P65 monoclonal antibody, rabbit-anti-rat vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1)monoclonal antibodies, and 3,3-diaminobenzidine (DAB) kit were purchased from Wuhan Boster Biological Technology Co., Ltd.METHODS: ① Interventions: The rats were randomly divided in the saline control group (n =25), dexamethasone group(n =25) and normal comea group (n =5). Corneal neovascularization using nylon suture was induced in rats in the saline control group and dexamethasone group, and then saline and dexamethasone was dropped to the right eye of the rats respectively, 2 drops for each time, 3 times a day for 18 days. Not any treatment was given to the rats in the normal cornea group. ② Evaluations: The score of corneal neovascularization was evaluated in the saline control group and dexamethasone group at 1, 3, 7, 12 and 18 days postoperatively. Corneal sections were prepared to observe the histological changes of cornea under light microscope; The expressions of NF-кB, VEGF and ICAM-1 were detected with immunohistochemical staining.MAIN OUTCOME MEASURES: ① Score of corneal neovascularization; ② Histological changes of cornea; ③ Expressions of NF-кB, VEGF and ICAM-1 in cornea.RESULTS: ① Score of corneal neovascularization: The corneal neovascularization was obviously inhibited, and scores of corneal neovascularization at different time points were all significantly lower than those in the saline control group (P ＜0.05-0.01). ② Histological changes of cornea: In the dexamethasone group, corneal neuvascularization and the infiltration of inflammatory cells after suture were obviously alleviated as compared with those in the control group, and the corneal structures in each layer were relatively complete. ③ Expressions of NF-кB, VEGF and ICAM-1 in cornea: In the dexamethasone group, the expressions of NF-кB, VEGF and ICAM-1 at each time points were all lower than those in the saline control group (P ＜ 0.05). The intensity of the expression of NF-кB was positively correlated with the score of corneal neovascularization and the expressions of ICAM-1 and VEGF (r =0.961, 0.922, 0.958, P ＜ 0.01).CONCLUSION: NF-кB is involved in the formation of corneal neovascularization possibly through upregulating the expressions of its downstream genes (VEGF, ICAM-1). Dexamethasone can inhibit the expressions of many factors related to corneal neovascularization regulated by NF-кB, including cytokines and adhesion molecules, through reducing the activity of NF-кB, and then suppresses the occurrence and development of corneal neovascularization.