1.Preliminary study on activities of flavonoides extracted from flowers of Lonicera japonica Thunb. on some index of lipidemia
Pharmaceutical Journal 2005;0(1):15-18
Studying effect of total flavonoid extracts from Lonicera japonica Thunb on some index of lipidemia. Rats were drunk cholesterol at a dose of 0.5g/kg/24hours for 6 consecutive weeks in order to cause hyperlipidemia. The result showed that: flavonoid extracted from Lonicera japonica Thunb at a dose of 0.45g/kg and 0.9g/kg decreased plasma triglyceride, total cholesterol, LDL-C and increased HDL-C in white rats drunk cholesterol in vitro. The effects of flavonoid at the dose of 0.45g/kg and 0.90g/kg on some index of lipidemia were the same. There was no differences in increasing body weight of rats in all studied rat groups
Flavonoids
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Flowers
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Lonicera
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Hyperlipidemias
2.Anti-inflammatory effects of flavonoids from Lonicera japonica Thunb. Caprifoliaceae in combination with ?-amylase
Pharmaceutical Journal 2005;347(3):8-11
In vitro, flavonoids from Lonicera japonica Thunb flavonoids stimulate the catalysis of hydrolysis starch. This stimulating effect depended on the concentration of Lonicera japonica flavonoid but their in vitro stimulations weren’t strong. The anti-inflammatory effects were stronger when combining flavonoid with -amylase on dextran-induced edema mouse legs. So active catalysis stimulation of -amylase could be associated with anti-inflammation process; with unclear mechanisms. This result suggested the need of further research of anti-inflammatory drugs according to combine of traditional medicine and anti-inflammatory enzyme
Flavonoids
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Anti-Inflammatory Agents
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Lonicera
3.Observation and comparision on morphological characteristics of pollen of Lonicera japonica in different cultivars.
Fang ZHANG ; Yongqing ZHANG ; Fengqin ZHOU ; Bingqing ZHENG
China Journal of Chinese Materia Medica 2011;36(10):1266-1268
OBJECTIVETo compare morphological characteristics of pollen of Lonicera japonica in different cultivars cultivated in Shandong and provide a basis for distinguishing different cultivars and selecting fine breeding.
METHODThe scanning electron microscope (SEM) was applied to observe and compare the outside characteristics of pollen,and the data were analyzed by SAS 9.1 software.
RESULTSome difference was found among pollen size, aperture length and the density of spine. The biggest pollen size and aperture length is Dajizhao with 61.97 microm polar axis and 61.79 microm quarter major axis and 18.03 microm aperture length,and the smallest is Honggengzi with 57.46 microm polar axis and 57.29 microm quarter major axis and 18.03 microm aperture length.
CONCLUSIONThe morphological characteristics of pollen can provide a basis for distinguishing different cultivars of L. japonica.
Lonicera ; anatomy & histology ; Pollen ; anatomy & histology
4.Study on characteristics of non-glandular hairs of cultivated Lonicera japonica.
Shan-shan ZHANG ; Yuan YUAN ; Lu-qi HUANG ; Ping CHEN
China Journal of Chinese Materia Medica 2015;40(3):390-394
We collected 22 cultivated population of Lonicera japonica from 17 areas. The characteristics of non-glandular hairs were observed and measured by the scanning electron microscopy. The principal components analysis and correlation analysis were conduct based on length and density of L. japonica. The results showed a significant negative correlation between length and density of non-glandular hairs, and the characteristics of non-glandular was not corrrelated significantly with latitude. The correlation results indicated that the density was a key to separate "Damaohua" and "Jizhuahua". The contribution of climate and soil was important to the cultivated population. This reminded that the characteristics of non-glandular hairs were affected by environmental and genetic interaction.
Lonicera
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anatomy & histology
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growth & development
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ultrastructure
5.Analysis of genetic diversity and genetic relationships of Lonicera macranthoides cultivars.
Daxia CHEN ; Xue ZHANG ; Longyun LI
China Journal of Chinese Materia Medica 2012;37(13):1912-1916
OBJECTIVETo study genetic diversity and genetic relationships among Lonicera macranthoides cultivars.
METHODFive cultivars were estimated by ISSR and SRAP. The data of amplified bands were analyzed by Treeconw software. The system diagram of genetic relationship was built by UPGMA.
RESULTTwenty ISSR primers amplified 186 bands with 103 (54.63%) polymorphic bands and 58 SRAP primer combinations amplified 591 bands with 347(55.46%) polymorphic bands. Genetic distance ranges were 0.058 4-0.230 8 (by ISSRs) and 0.1071-0.2611 (by SRAPs). Both ISSR and SRAP analyses revealed a middle level of genetic diversity in L. macranthoides cultivars. The dendrograms based on SRAP and ISSR markers were not all the same.
CONCLUSIONThe genetic diversity of L. macranthoides cultivars is middle. ISSR and SRAP markers can be effectively applied to genetic analysis in L. macranthoides cultivars.
Genetic Variation ; Lonicera ; genetics ; Polymorphism, Genetic ; Software
6.Research on remote sensing recognition of wild planted Lonicera japonica based on deep convolutional neural network.
Ting-Ting SHI ; Xiao-Bo ZHANG ; Lan-Ping GUO ; Zhi-Xian JING ; Lu-Qi HUANG
China Journal of Chinese Materia Medica 2020;45(23):5658-5662
Identification of Chinese medicinal materials is a fundamental part and an important premise of the modern Chinese medicinal materials industry. As for the traditional Chinese medicinal materials that imitate wild cultivation, due to their scattered, irregular, and fine-grained planting characteristics, the fine classification using traditional classification methods is not accurate. Therefore, a deep convolution neural network model is used for imitating wild planting. Identification of Chinese herbal medicines. This study takes Lonicera japonica remote sensing recognition as an example, and proposes a method for fine classification of L. japonica based on a deep convolutional neural network model. The GoogLeNet network model is used to learn a large number of training samples to extract L. japonica characteristics from drone remote sensing images. Parameters, further optimize the network structure, and obtain a L. japonica recognition model. The research results show that the deep convolutional neural network based on GoogLeNet can effectively extract the L. japonica information that is relatively fragmented in the image, and realize the fine classification of L. japonica. After training and optimization, the overall classification accuracy of L. japonica can reach 97.5%, and total area accuracy is 94.6%, which can provide a reference for the application of deep convolutional neural network method in remote sensing classification of Chinese medicinal materials.
Lonicera
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Neural Networks, Computer
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Remote Sensing Technology
7.Study on constituents of essential oil from Lonicera fulvotomentosa in different collected periods.
Lihua HUANG ; Daoping WANG ; Xun CHEN
China Journal of Chinese Materia Medica 2011;36(16):2230-2232
OBJECTIVETo extract and identify the chemical constituents of essential oil from Lonicera fulvotomentosa in different collected periods (bud, Silver-flower and Golden-flower periods).
METHODExtracts in three different collected periods were subjected to GC-MS analysis for determination of their chemical constituents.
RESULTThe 29, 34 and 28 kinds of chemical constituents corresponding to the above three periods were found, and 44 kinds of compounds were identified. The relative content of every chemical constituents in each essential oil was obtained by area normalization method.
CONCLUSIONThe O-tolyl isocyanide was detected from essential oil of Lonicera for the first time. The result indicated that the highest relative content in essential oil in the three periods is alcohol substance and the second is ester and aldehyde. Many common constituents in the essential oil from L. fulvotomentosa, including linalool, hyacinthin, O-tolyl isocyanide, geraniol, methyl anthranilate, and so on, all could be detected in the three periods. However, the differences of their relative content are obvious.
Gas Chromatography-Mass Spectrometry ; Lonicera ; chemistry ; Oils, Volatile ; analysis
8.Application and study of HPLC fingerprint of Lonicera japonica for identification of geo-authenticity.
China Journal of Chinese Materia Medica 2008;33(9):996-998
OBJECTIVETo establish a chromatographic fingerprint of Lonicera japonica and apply it to identify geo-authentic of L. japonica.
METHODThe HPLC was applied in chromatographic separation and data were analysed by "computed aided similarity evaluation" software.
RESULTThere exited distinct difference of chemical components group between and non-authentic samples.
CONCLUSIONThe established HPLC fingerprint can be used for the identification of geo-authentic of L. japonica.
Chromatography, High Pressure Liquid ; methods ; Geography ; Lonicera ; chemistry ; classification
9.Effect of exogenous GA₃ and DPC on active components of Lonicera japonica and its mechanism.
Yan-Xia ZHU ; Shuang-Shuang QIN ; Chao JIANG ; Jian YANG ; Qing-Hua WU ; Ying LIANG
China Journal of Chinese Materia Medica 2018;43(24):4817-4823
To reveal the effect of plant growth regulator GA₃ and DPC on the active components and its possible mechanism of Lonicera japonica. GA and DPC were applied at the stage of flower bud differentiation, and the content of active ingredients was measured by LC-MS-MS, the content of endogenous hormones were measured by ELISA, and the expression of key enzyme enes expression was determined by qRT-PCR. The level of endogenous hormone GA₃, IAA, ZR, DHZR and iPA in the GA treatment group was significantly improved, the expression of C4H1, C4H2, 4CL1 and HQT2 were also significantly increased. The content of chlorogenic acid, luteolin, luteoloside, isoquercetin and caffeic acid increased significantly. Spraying DPC did not affect or inhibit the accumulation of active components of L. japonica. Spraying GA can increase the content of endogenous GA₃, thus enhance the expression of C4H1, C4H2, 4CL1 and HQT2, and then increase the content of chlorogenic acid and luteolin.
Chlorogenic Acid
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Lonicera
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Plant Growth Regulators
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Tandem Mass Spectrometry
10.Effect of calcium on ion contents and expression of photosynthetic related genes in honeysuckle under salt stress.
Lu-Yao HUANG ; Zhuang-Zhuang LI ; Long-Tai JU ; Pan-Pan SUN ; Guo-Zhen WU ; Yu-Meng WU ; Jia LI
China Journal of Chinese Materia Medica 2019;44(12):2452-2458
Exogenous calcium can enhance the resistance of certain plants to abiotic stress. Research have demonstrated that exogenous calcium could enhances the resistance of honeysuckle under salt stress by promoting the transmission of photosynthetic electrons.The aim of this study was to investigate the effects of exogenous calcium on the contents of Na~+,K~+,Ca~(2+),Mg~(2+)and the expression of photosynthetic related genes Cab and rbc L. In this study,we used ICP-OES to analysis ion contents and used qRT-PCR to analysis the expression patterns of Cab and rbc L. The results showed that CaCl_2 significantly enhanced the K~+-Na~+,Ca~(2+)-Na~+,Mg~(2+)-Na+ratio of honeysuckle treated with 50 and 100 mmol·L~(-1) NaCl. Meanwhile,Cab and rbc L were significantly up-regulated under short-term salt stress,and CaCl_2 promoted this trend. From the two gene expression patterns,rbc L rapidly up-regulated on the first day of stress and then decreased,and was more sensitive to environmental changes. In summary,exogenous calcium could alleviate salt stress and increase plant development by increasing intracellular K~+-Na~+,Ca~(2+)-Na~+,Mg~(2+)-Na+ratio,and the transient overexpression of Cab and rbc L.
Calcium
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physiology
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Cations
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analysis
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Lonicera
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physiology
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Photosynthesis
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Salt Stress