1.The prevention of acute graft versus host disease by Rhodamine 123-mediated photodynamic therapy
Huilan ZENG ; Kanger ZHU ; Jianwei JIANG ; Huaimin GU ; Jiye CAI ; Longqiu CHENG ; Zhigang YANG ; Meizhen ZHENG
Chinese Journal of Pathophysiology 2000;0(11):-
AIM: To evaluate the effect and safety of Rhodamine 123 (Rh123)-mediated photodynamic treatment (PDT) on acute graft versus host disease. METHODS: An acute graft versus host disease (aGVHD) mice model was established using C57B/6 mice as donors and BALB/c mice as recipients. Mixed lymphocytic cells were cultured with Rh123 (50 nmol/L) and irradiated by argon laser 30 mW/cm2 for 3 min, then transplanted to BALB/c recipient mice mixed with donor bone marrow. Hepatopoietic recovery, aGVHD occurrence, survival time after transplantation and pathological changes were observed. In addition, CD3+CD69+ positive rates of MLC were examined by flowcytometry. RESULTS: Occurrence of aGVHD decreased, degree of pathological manifestation became milder, survival rates were higher than non PDT groups. CD3+CD69+ rates of MLC cells treated with photodynamic therapy (PDT) and cultured for 24 h significantly decreased. CONCLUSION: Rh123-mediated PDT can effectively prevent aGVHD of allogeneic bone marrow transplantation in mice.
2.Change of p-CREB after surgical incisional pain in rat spinal cord.
Yulong CUI ; Junmei XU ; Ruping DAI ; Changqi LI ; Dan LIU ; Longqiu YANG ; Juan WANG
Journal of Central South University(Medical Sciences) 2010;35(8):833-839
OBJECTIVE:
To explore the role of phosphorylation of cAMP response element binding protein (CREB) in the incision-induced pain hypersensitivity.
METHODS:
A longitudinal incision was made in one plantar hind paw of isoflurane-anesthetized rats. Spinal cords were removed at various postoperative time after behavior test. Phosphorylation of CREB was determined by immunohistochemistry and double-labeling immunofluorescence. Morphine and gabapentin were intraperitoneally injected before the behavior test and were used to determine the interaction between phosphorylation of CREB and morphine and gabapentin.
RESULTS:
After the hind-paw incision, phosphorylation of CREB was enhanced in the ipsilateral lumbar spinal cord (P<0.05). The enhancement of p-CREB was mainly in the neurons in the dorsal horn of the spinal cord. All these were shown by double-labeling technique and p-CREB was mainly in the neurons. Intraperitoneal injection of morphine prevented the increased phosphorylation of CREB in the spinal cord and inhibited the mechanical allodynia induced by the incision (P>0.05). Gabapentin didn't inhibit the phosphorylation of CREB (P<0.05) but partly inhibited the mechanical allodynia.
CONCLUSION
Incision induces the phosphorylation of CREB in the spinal cord, and the increase of p-CREB is mainly in the neurons. Phosphorylation of CREB in the spinal cord contributes to the pain hypersensitivity induced by surgical incision.
Animals
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Cyclic AMP Response Element-Binding Protein
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metabolism
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Female
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Foot
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surgery
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Neurons
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metabolism
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Pain Threshold
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Pain, Postoperative
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metabolism
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Phosphorylation
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Rats
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Rats, Sprague-Dawley
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Spinal Cord
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metabolism