1.Inhibition of proliferation of human hepatocellular carcinoma cell lines by TNBG in vitro
Journal of Chongqing Medical University 1986;0(03):-
Objective:To investigate the effect of TNBG on proliferation of human hepatocellular carcinoma cell lines SMMC-7721 and HepG2.Methods:MTT assay was used to test the effects of anti-proliferation.The flow cytometric analysis was used to detect the cell cycle distribution.Western blot was adopted for detecting the expression of cyclinB1 and p34 cdc2 .Results:Anti-proliferation effects were observed in SMMC-7721 and HepG2 after intervention of TNBG for 72 hours,and the effects were in concentration-dependent manners.The cells showed S and G2/M arrest in both of cell lines,and apoptosis was induced only in HepG2.The expression of cyclinB1 was inhibited,while p34 cdc2 was not changed.Conclusion:TNBG can inhibit the proliferation of tumor cells,which is due to G2/M arrest caused by expression inhibition of cyclinB1 induced by TNBG.
2.A clinical research on the postoperative recurrentive factors of oral squamous cell carcinoma
Journal of Practical Stomatology 1996;0(02):-
Objective:To analyze the postoperative recurrentive factors of oral squamous cell carcinoma.Methods: 673 patients of primary oral squamous cell carcinoma were involved. The focus location, growth type, T stage, lymphonodus metastasis, treatment, pathological grading and resection mode of mandible were evaluated to determine the prognosis of patients.Results:The postoperative recurrence rate was relevant to the focus location, growth type, T stage, lymphonodus metastasis and pathological grading, while the treatment and resection mode of mandible had no effects on the recurrence rate. Conclusion: It's of great importance to estimate all kinds of clinicopathologic factors and perform preventive treatments during the clinical work to promote the operative cure rate and survival rate of patients with oral squamous cell carcinoma.
3.The effects of isoproterenol on the proliferation of primarily cultured rat submandibular gland cells
Guilin HUANG ; Longjiang LI ; Xueying LI
Journal of Practical Stomatology 2000;0(05):-
Objective:To study the effects of isoproterenol(Iso) on the proliferation of cultured submandibular gland cells of SD rats. Methods:Submandibular gland cells of SD rats were primarily cultured. The cells were exposed to Iso at 10 -3 g/L continuously for 8 days or intermittently(2 h each day for 8 days). The cell proliferation was sutdied by bromodeoxyuridine Brdu labeling and cell counting.Results:Iso increased cell proliferation(P
4.Allogeneic transplantation of myoblasts seeded on typeⅠcollagen sponges in rats
Guilin HUANG ; Longjiang LI ; Xueying LI
Journal of Practical Stomatology 2001;0(01):-
s Objective: To study the feasibility of allogeneic tra ns plantation of myoblasts of SD rats for the reconstruction of facial muscle de fects. Methods: Myoblasts obtained from the forelimbs and hindlimbs of neonatal SD rats were purified and cultured. 5?10 4 myoblasts w ere seeded onto each of type Ⅰcollagen sponges in the size of 1.5 mm?0.8 mm? 0.5 cm. The myoblasts/sponge constructs were transplanted into the facial muscl e defects of syngeneic mutured rats and observed with microscopy,immunohistoche mistry and electromyography (EMG).Result:Myoblasts attac hed and kept alive on type I collagen for at lest 3~4 days in culture. 4 and 8 w eeks after transplantation,muscle like tissue with blood vessles was observed i n the implants. Expression of actin and myosin in the implants was similar to th at in the control muscle.8 weeks after implantation,the spike wave (mV) on the g rafted side and control side was 0.7?0.3 and 2.7?0.6 ( P
5.Preparation and biocompatibility of acellular trachea matrix
Guilin HUANG ; Longjiang LI ; Xueying LI
Journal of Practical Stomatology 2001;0(03):-
Objective:To prepare an acellular matrix from trachea of rabbits and SD rats, and to investigate its biocompatibility.Methods: A modified detergent and enzyme link extraction procedure was performed to remove cells from the trachea of SD rats and rabbits. The histology, topography of inner-surface and biocompatibility were studied by morphological observation,cell culture and in vivo transplantation respectively.Results:The acellular trachea matrix did not inhibit the growth and amylase secretion of allogenic salivary gland cells cultured on it.The allogenically transplanted acellular trachea matrix did not result in inflammation reaction of the host tissue,could integrate with surrounding tisses.Conclusion:The acellular trachea matix is biocompatible.
6.The effect of immune reaction induced by alginate on parotid acinar cells in vitro
Wei HE ; Jilian LV ; Longjiang LI
Journal of Practical Stomatology 2009;25(6):779-782
Objective: To explore the effect of immune reaction induced by alginate on parotid acinar cells in vitro. Methods: Rabbits were immunized from the conjugated alginate- BSA (1.0 mg/kg) by 40-days routine immunity method. ELJSA method was used to examine the titration (valence) of anti-alginate serum. Five groups (group A: contrast, group B: BSA, group C; alginate, group D: anti-alginate serum, group E; alginate + anti-alginate serum) were examined by MTT method at four time points( 1, 6,12 and 24 h). The growth and morphology of parotid acinar cells were observed under inverted phase contrast microscope and scanning electron microscope. Results: Antibody-serum was acquired by routine immunity method, and the titration (valence) of anti-alginate serum was 1: 400. MTT results showed that the proliferation of parotid acinar cells had been limited at 24 h( P <0.05), the other three time points showed no difference. Under inverted phase contrast microscope, a few of acinar cells whose membranes were destroyed after 12 h, some cell contents leaked out. The holes in membrane could be seen early at 6h under scanning electron microscope. Most of the acinar cells were broken at 24 h. Conclusion: The antibody-serum to alginate and immunized rabbit was acquired by routine immunity method. The immune reaction induced by alginate can destroy parotid acinar cells in vitro.
7.Expression of nucleolar organizer region associated proteins of Tlymphocytes in the patients with salivary gland tumor
Liu LIU ; Yuming WEN ; Longjiang LI
Journal of Practical Stomatology 2001;0(01):-
s Objective:To study the expression of nucleolar orga ni zer region associated protein (Ag NORs) of T lymphocytes in the patients with tumor of salivary gland. Methods:The expression of Ag NORs of T lymphocytes in 60 normal adults, 56 patients with benign salivary gla nd tumor and 45 patients with malignant salivary gland tumor was analysed by Ag stainning and KL imaging system. Results: The ratio of Ag NORs positive area to nuclear area of T lymphocytes in normal adults,pati ents with benign tumor and those with malignant tumor were 7.88?0.10, 5.71?0.1 3 and 4.21?0.12 respectively ( P
8.Analysis of HLA-DR expression in relation to the development of oral squamous cell carcinoma
Shaoping ZHANG ; Longjiang LI ; Xin HUANG
Journal of Practical Stomatology 2001;0(01):-
Objective: To observe HLA DR expression in different s tages of oral squamous cell carcinoma (OSCC) and to study the clinic significan ce of the abnormal HLA DR expression in primary OSCC. Method: HLA DR expression was detected by immunohistochemistry method in 26 cas es of histologically normal oral epithelia, 8 leukoplakia, 32 primary focuses an d 12 metastasis focuses of OSCC. Results: HLA DR express ion in primary OSCC focuses was significantly higher than that in normal epithel ium( P 0.05). Conclusion: Although HLA DR expression is frequently obs erved in the development of OSCCs, it can not be regarded as one of the indepen dent prognosis factors.
9.Influence of micrometastasis in pathologically negative lymph node on staging and prognosis of oral squamous cell carcinoma
Hongwei ZHAO ; Longjiang LI ; Wenyang XIE
Journal of Practical Stomatology 2001;0(03):-
Objective: To study the influence of micrometastasis in lymph node on the prognosis of oral squamous cell carcinoma(OSCC).Methods: In 36 patients micrometastasis in pathologically negative lymph nodes were tested by immunohistochemical cytokeratin(CK) examination and the relationship between CK+ and survival time of the patients was analyzed. Results: The survival time(month) of CK+ and CK- patients was 36.76?6.91 and 47.47?11.35 respectively(P=0.002).Multivariate analysis of Logistic regression model showed that micrometastasis in lymph node (P=0.044)and histologic grade (P=0.040) were regarded as independently prognostic factors while clinical stage (P=0.236)did not. Conclusion: The detection of micrometastasis in the lymph nodes may serve as a supplement to the present staging system of OSCC. The prognosis of patients with micrometastasis is poorer than those without micrometastasis.
10.A preliminary study on the proliferation of endothelial cells of human hemangioma
Xiaoming DAI ; Longjiang LI ; Yuming WEN
Journal of Practical Stomatology 2001;0(03):-
Objective: To study the role of simian virus 40 large T antigen(SV40LTag) and telomeras in the proliferation of endothelial cells in hemangioma. Methods: The expression of SV40LTag was examined in 100 cases of hemangioma with immunohistochemistry, telomerase activity and telomeric length were measured by RT-PCR based telomeric repeat amplification protocol(TRAP) assay, telomeric restriction fragment (TRF) analysis and chemiluminescence in situ hybridization in 50 cases of hemangioma and 10 of squamous cell carcinoma. A sample of embryonic kidney endothelia was used as the contrdol. Results: SV40LTag expression was observed in 45 out off the 100 cases of hemangioma. Telomerase expression was found in 27 out off the 50 cases of hemangioma and all the 10 of squamous cell carcinoma. The telomeric length in hemangioma varied from 6.8 to 13.2 kb, 10.5-13.5 kb in squamous cell carcinoma and 12.4 kb in embryonic kidney endothelia. Conclusion: Transformation of endothelia by simian virus 40 may contribute to the occurring of hemangioma, telomerase may play a role in the proliferation of the endothelial cells in hemangioma.