Objective To explore the molecular pathological mechanism and treatment of retinoic acid syndrome(RAS).Methods SDF-1? of health adult lung was measured by RT-PCR,CXCR4 on the cell membrane of APL specialized by arsenic trioxide(APL/ATO)were tested by FCM,and we used the rotary cell culture system(RCCS)to build the model of simulated experiment in vitro of APL/ATO infiltrating into the human lung;observe if Dex,Ara-C and DNR can influence the ability of APL/ATO in adhesion,transplantation and infiltration.Results The APL/ATO could evidently infiltrate into human lung,mean fluorescence intensity(MFI)of CXCR4 on the cell membrane of APL/ATO was 28.77?1.05,which was much higher than the unspecialized APL(9.20?4.14).Contrast to control cells,Dex could dramatically restrain the ability of APL/ATO in adhesion and transference [(29.91?2.70)% vs(48.20?5.00)%,30.01?5.01 vs 60.10?3.02],while Ara-C and DNR could distinctly depress the ability of APL/ATO in adhesion,transplantation and infiltration[(30.10?3.00)%﹑(32.20?2.20)% vs(48.20?5.00)%;28.01?5.00,24.02?4.01 vs 60.10?3.02;18.20?3.56,16.01?3.25 vs 46.01?4.05].Conclusion High expression of CXCR4 on APL/ATO and SDF-1?in the lung may be one of the molecular mechanism of the lung infiltration and RAS;DEX、Ara-C and DNR can restrain the ability of APL/ATO in adhesion,transplantation and infiltration.

Objective To design a system that is many X-ray machines automatic operation,timely exposure to realize the sharing of power supply. Methods A circuit is designed based on the normal hospital conditions and go with proper control algorithm. The fifty-one series SCM were made use of in the hardware circuits,and validated by X-ray machine simulation tests which simplify the X-ray exposure to the process of relay controlling,which software is based on interrupt mechanism. Results The program could effectively control the exposal of many X-ray machines and the priority was indicated by the experiments. Conclusion Under the prerequisite of the system does not affect the exposure parameters of X-ray machine accuracy,the system can realize a number of X-ray machine's power-sharing,at the same time allow only a single X-ray exposure and use of the interrupted source. And it can make exposure many original X-ray machines can become closely related with high security,proper order and high performance,which has prominent benefits on areas with bad power and equipment conditions.

Objective To develop an HPLC method for the quantitative analysis of tricin in malt. Methods HPLC method was used. Analysis was performed on Spherigel C_ 18 column (150 mm?4.6 mm, 5 ?m) with acetonitrile-water-tetrahydrofuran-methanoic acid (26∶74∶10∶1) as mobile phase. The detection wavelength was 350 nm, flow rate was 1.0 mL/min, and the analysic volumn was set at 20 ?L. Results The calibration curve of tricin was linear in the range of 0.22-2.15 mg/L (r=0.999 8). The average recovery rate of tricin was 97.4% (RSD=1.7%) (n=9). Conclusion The method can be used to determine tricin in raw malt quantitatively.

OBJECTIVETo set up a novel method for fast analysis of active components in water extracting process of Paeonia lactiflora with near infrared spectroscopy (NIRS).

METHODHPLC was used as the reference method to determine the content of Paeoniflorin and a multivariate calibration model based on PLS algorithm was developed to analyze the correlation between the spectra and the corresponding values determined by the reference method.

RESULTThe correlation coefficient of the calibration model was 0.996 2, and the predicted coefficient was 0.989 5. The RMSEC and RMSEP were 0.109 g x L(-1) and 0.138 g x L(-1), respectively, and the RSEP was 5.6%.

CONCLUSIONThe method mentioned above is proved to be convenient, rapid and nondestructive, accurate and reliable, and is applicable for fast analysis and monitoring of active components in extraction process of traditional Chinese medicine.

Paeonia ; chemistry ; Plant Extracts ; chemistry ; Quality Control ; Spectroscopy, Near-Infrared ; methods

OBJECTIVETo study the optimum technical conditions of extracting Hydroxysafflor yellow A (HSYA) from Carthmus tinctorius by multi-stage countercurrent extraction technology.

METHODThe effects of extraction time of each stage, extraction temperature, ethanol concentration and solid-liquid ratio (g x mL(-1)) on extraction yield of HSYA were studied by orthogonal test design and the comparison of other extraction methods were presented.

RESULTExtraction time and solid-liquid ratio had significant influence on the extraction yield, and the optimum parameters were as follows: Extraction time of each stage was 120 min, solid-liquid ratio was 1 : 10 (g x mL(-1)), ethanol concentration was 30%, and extracted at room temperature. Under the optimum conditions, the extraction yield of HSYA was 1.56% and the purity of the extract was 6.06%. Compared with the traditional extraction method and the ultrasonic extraction method of the pharmacopoeia, the extraction yield was increased by 6.12% and 9.09%, the purity of extract was increased by 42.9% and 27.0%, respectively.

CONCLUSIONThe multi-stage countercurrent extraction technology has many advantages such as simple operation, less solvent consumption, higher extraction yield and purity of extract and it has wide industrial application prospect.

Carthamus ; chemistry ; Chalcone ; analogs & derivatives ; analysis ; isolation & purification ; Countercurrent Distribution ; methods ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; Quinones ; analysis ; isolation & purification

OBJECTIVETo observe the impact of PDE5shRNA on cardiac remodeling and heart function following myocardial infarction in mice.

METHODSMyocardial infarction (MI) was induced in mice by left coronary artery ligation. Mice were randomly assigned to sham group (n = 6), PDE5shRNA group (n = 12), common adenovirus group (n = 15) and DMEM group (n = 8). Four weeks post-MI, the survival rate was evaluated. Cardiac function was examined by echocardiography. HE staining and Masson staining were used to evaluate the myocardial infarction size and fibrosis. The number of blood vessels was evaluated by immunohistochemistry, PDE5 protein expression in the left ventricular was detected using Western blot, level of cGMP or PKG activity in the left ventricle was evaluated with ELISA.

RESULTSFour weeks post-MI, all mice survived in the sham group, 3(37%) mice died in the DMEM group, 1 (8%) died in the PDE5shRNA group and 5 died in the common adenovirus group (33%). Infarct size was significantly reduced in PDE5shRNA group compared with the common adenovirus group and DMEM group [(25.4 ± 2.9)% vs. (42.0 ± 3.2)% and (43.4 ± 2.6) %, P < 0.05]. Cardiac function was significantly improved in PDE5shRNA group compared to common adenovirus group and DMEM group[LVFS: (21.1 ± 3.7)% vs. (14.2 ± 2.9)% and (14.22 ± 2.91)%, all P < 0.05; LVEF: (48.2 ± 7.1)% vs. (34.6 ± 6.2)% and (38.1 ± 2.8)%, all P < 0.05; LVESD: (3.87 ± 0.45) mm vs.(4.91 ± 0.62) mm and (4.63 ± 0.37) mm, all P < 0.05]. The blood vessel density was also higher in PDE5shRNA group compared with common adenovirus group (infarct area:14.3 ± 2.0 vs. 6.6 ± 1.2, P < 0.05; periinfarct area: 23.6 ± 2.1 vs. 13.7 ± 2.4, P < 0.05). Compared with common adenovirus group, level of PDE5 was significantly downregulated and level of cGMP or PKG was significantly upregulated in PDE5shRNA group (all P < 0.05).

CONCLUSIONSPresent study suggests PDE5shRNA improves cardiac function and attenuates cardiac remodeling through reducing infarction size and cardiac fibrosis and these beneficial effects are possibly mediated by activating cGMP/PKG signaling pathway.

Adenoviridae ; genetics ; Animals ; Cyclic Nucleotide Phosphodiesterases, Type 5 ; genetics ; Disease Models, Animal ; Heart Failure ; etiology ; therapy ; Male ; Mice ; Mice, Inbred C57BL ; Myocardial Infarction ; complications ; RNA, Small Interfering ; genetics ; Ventricular Remodeling