Objective To discuss the clinical value of Mutislice CT angiography (MSCTA)in cervical vascular diseases. Methods 60 patients highly suspected suffering cervical vascular diseases,were implemented 16-slices spiral CT angiography of cervical vascular,and blood vessel reconstruction and analysis were done subsequently. Results 43 cases with cervical vascular abnormality were presented in the 60 patients,who were implemented MSCTA. Positive ratio was 71.7%. 171 pieces vessel stenosis were detected among 240 pieces carotid artery and vertebra artery, and 4 cases carotid artery aneurysm and 1 case AVM were found also. Conclusion MSCTA is a convenient and shortcut modality to inspect cervical vascular diseases, and the blood vessel can be observed 3-dimensionally and in any direction in high quality reconstruction images.

Objective: To explore the role of angiotensin receptor type I (AT1)-calcineurin (CaN) signaling pathway in transient outward potassium ion channel (Ito) remolding in hypertrophic atrial myocytes of neonatal rats. Methods: 1 day old neonatal rats’ atrial myocytes were isolated and the cells were divided into 4 groups:①Control group, normal cells were cultured for 24 h,②Stretching group, the cells were cultured for 24 h with mechanical stretching to induce hypertrophy,③Telmisartan group, the cells were treated by telmisartan at 1 μmol/L for 1 h, then cultured for 24 h and ④Cyclosporin-A (CsA) group, the cells were treated by CsA at 0.25 μg/ml for 1 h, then cultured for 24 h. The ratios of protein/DNA in myocytes were compared between Control group and Stretching group, cell hypertrophy was deifned by mRNA expression of atrial natriuretic peptide (ANP). Ito changes were detected by whole-cell patch clamping technique, proteins expressions of Kv4.3 and CaN A subunit were examined by Western blot analysis. Results: Compared with Control group, Stretching group showed obviously decreased Ito density and Kv4.3 protein expression, while increased CaN A protein expression; Compared with Stretching group, the above effects were reduced in Telmisartan group and CsA group. Conclusion: AT1-CaN signaling pathway was involved in the regulation of Ito channel remodeling in hypertrophic atrial myocytes of neonatal rats.

Objective To investigate the effects of galangin on angiogenic activity of oxidized low-density lipoprotein(ox-LDL)-induced human aortic endothelial cells(HAECs)and explore the underlying mechanisms.Methods HAECs incubated with 10,20,40,and 80 μmol/L galangin for 24 h were assessed for cell viability changes using MTT assay to determine the cytotoxicity of galangin.HAECs treated with 5 mg/mL ox-LDL and incubated with 20 and 40 μmol/L galangin for 24 h,and the cells overexpressing lncRNA H19 and incubated with 40 μmol/L galangin for 24 h were examined for lncRNA H19 level with qRT-PCR.The migration and tube formation capacity of the cells were observed using scratch assay and angiogenesis assay,and ROS levels in the cells were detected with flow cytometry.The protein expression levels of VEGFA,MMP-2 and MMP-9 in the treated cells were detected with Western blotting.Results Galangin at 10,20,or 40 μmol/L produced no obvious toxicity(P>0.05),whereas 80 μmol/L galangin significantly inhibited the viability of HAECs(P<0.01).Treatment with ox-LDL significantly increased the expression of lncRNA H19 in HAECs.Galangin significantly lowered lncRNA H19 expression in ox-LDL-induced HAECs,suppressed cell migration,angiogenesis and ROS production level,and reduced the protein levels of VEGFA,MMP-2 and MMP-9(P<0.01).The effects of galangin were blocked by overexpression of lncRNA H19 in the cardiomyocytes.Conclusion The therapeutic effect of galangin for atherosclerosis is mediated by inhibiting lncRNA H19 expression to reduce ox-LDL-induced migration,oxidative stress,and angiogenesis of HAECs.

Objective To investigate the effects of galangin on angiogenic activity of oxidized low-density lipoprotein(ox-LDL)-induced human aortic endothelial cells(HAECs)and explore the underlying mechanisms.Methods HAECs incubated with 10,20,40,and 80 μmol/L galangin for 24 h were assessed for cell viability changes using MTT assay to determine the cytotoxicity of galangin.HAECs treated with 5 mg/mL ox-LDL and incubated with 20 and 40 μmol/L galangin for 24 h,and the cells overexpressing lncRNA H19 and incubated with 40 μmol/L galangin for 24 h were examined for lncRNA H19 level with qRT-PCR.The migration and tube formation capacity of the cells were observed using scratch assay and angiogenesis assay,and ROS levels in the cells were detected with flow cytometry.The protein expression levels of VEGFA,MMP-2 and MMP-9 in the treated cells were detected with Western blotting.Results Galangin at 10,20,or 40 μmol/L produced no obvious toxicity(P>0.05),whereas 80 μmol/L galangin significantly inhibited the viability of HAECs(P<0.01).Treatment with ox-LDL significantly increased the expression of lncRNA H19 in HAECs.Galangin significantly lowered lncRNA H19 expression in ox-LDL-induced HAECs,suppressed cell migration,angiogenesis and ROS production level,and reduced the protein levels of VEGFA,MMP-2 and MMP-9(P<0.01).The effects of galangin were blocked by overexpression of lncRNA H19 in the cardiomyocytes.Conclusion The therapeutic effect of galangin for atherosclerosis is mediated by inhibiting lncRNA H19 expression to reduce ox-LDL-induced migration,oxidative stress,and angiogenesis of HAECs.

Objectives:This study aimed to elucidate the incidence,clinical characteristics and prognosis of ST-elevation during radiofrequency catheter ablation for atrial fibrillation(AF). Methods:Consecutive patients who underwent radiofrequency catheter ablation for AF in Tongren Municipal People's Hospital,Qixingguan District People's Hospital of Bijie City and Guizhou Provincial People's Hospital from January 2021 to August 2023 were enrolled in this study.All patients underwent CARTO three-dimensional electroanatomical mapping and radiofrequency ablation via atrial septal approach under local anesthesia.The ST-elevation of electrocardiogram was analyzed.Follow-up was performed at 1,3,6,and 12 months after radiofrequency ablation.AF recurrence and duration,use of antiarrhythmic drugs,and incidence of death,thromboembolism,bleeding,and perioperative complications were evaluated. Results:ST-elevation was observed in 5 out of 798 patients(0.62%).ST-elevation occurred after transseptal puncture in three patients and during PentaRay multielectrode mapping in two patients.Blood pressure was significantly increased in three patients with transient ST-elevation(<20 min)and hemodynamic collapse occurred in two patients with persistent ST-elevation(>20 min).Catheter ablation of AF was completed in 4 patients,1 patient suffered severe hemodynamic disorders during radiofrequency catheter ablation,and the procedure was stopped immediately,this patient died from multiple organ system failure on the fifth day after failed radiofrequency catheter ablation,and the other 4 patients had no perioperative complications.The mean follow-up was(6±3)months,only 1 patient developed short atrial tachycardia,and the other patients had no recurrent atrial fibrillation and palpitation. Conclusions:Transient or persistent ST elevation can occur in patients during AF ablation.Early detection and rapid management are needed to prevent severe hemodynamic instability and cardiogenic death.

Objective: To compare the value of two 3D imaging reconstruction methods for left atria and pulmonary vein on guiding the catheter ablation for atrial fibrillation (AF). Methods: From January 2014 to January 2017, a total of 100 drug refractory paroxysmal AF patients were divided into left atria direct angiography group (n=50), and indirect angiography group (n=50). 3D CARTO system was applied for mapping and guiding the ablation procedure. Patients assigned to direct angiography group were treated as follows: intraoperative puncture of atrial septum, inject contrast agent directly into the left atrium, conduct left atrial and pulmonary venous rotation angiography, reconstruct three-dimensional image, integrate the image into real-time X-ray system to facilitate circumferential pulmonary vein isolation. Patients assigned into the indirect angiography group were treated as follows: inject contrast agent through the right ventricle, conduct delayed rotation angiography of the left atria and pulmonary vein to guide circumferential pulmonary vein fixation and ablation. The left atrial and pulmonary venous image acquisition, the operation and X-ray exposure time, the success rate and the incidence of complication of the two groups were compared. The patients were followed up for 3-6 months. Results: General clinical characteristics of the two groups were similar(all P>0.05). Ablation was successful in all 100 patients. The operation time[(112.0±21.4)min vs. (134.0±24.3)min]and X-ray exposure time((10.7±4.7)min vs. (15.8±5.2)min)were significantly lower in direct angiography group than in indirect angiography group (both P<0.01). There was no significant difference between the two groups in the immediate (86%(43/50) vs. 82%(41/50), P=0.59) and short-term (76%(38/50) vs. 72%(36/50), P=0.65) success rate and complication rate (1 aneurysm in the direct angiography group, 1 pericardial tamponade in the indirect angiography group). In-hospital mortality was zero percent. Conclusion It is safe and effective method to guide the radiofrequency catheter ablation of paroxysmal atrial fibrillation by reconstruction 3D image of left atrium and pulmonary vein. Compared with indirect angiography group, direct angiography group can improve the imaging quality of left atrium and pulmonary vein, decrease the X-ray exposure time of the ablation procedure.

【Objective】 To investigate the expression of CD36 antigen in Suzhou area, analyze the type of antigen deficiency and gene mutation, so as to provide references for the establishment of CD36 negative donor registry in Suzhou. 【Methods】 Anticoagulant whole blood samples (805 cases) were randomly collected from healthy blood donors in Suzhou Blood Center. The expression of CD36 antigen on platelet and monocyte was analyzed by flow cytometry to determine the type of CD36 deficiency. The gene mutation type of platelet CD36 antigen-deficient was performed by genomic DNA sequencing. 【Results】 The CD36 deficiency frequency on platelet was 2.48% (20/805), among which TypeⅠ(lacking CD36 expression both on platelet and monocyte) and TypeⅡ(lacking CD36 expression on platelet only) CD36 deficiency accounted for 10% (2/20) and 90% (18/20), respectively. CD36 gene mutations were found in 10 samples, including 3 cases of 329_330 d^elAC, 1 case of 1228_1239 d^elATTGTGCCTATT and 2 cases of 1163 A>T; 1 case of 329_330 d^elAC+ 1172_1183 d^elTATTGGTCAAGC and 287 G>C+ 329_330 d^elAC heterozygous mutation. In addition, 1 case of 745 A>G and 1 case of 806 C>T mutations were novel, and not yet reported. 【Conclusion】 Results showed that the frequency of CD36 antigen deficiency in Suzhou were similar to that reported in southern China, but the mutation sites were slightly different. The establishment of CD36 negative platelet registry could provide negative platelets for patients with transfusion reactions caused by anti-CD36 antibody and improve the effect of clinical platelet transfusion.