bFGF can promote endothelial cell to adhere to artificial polyurethane material. However the mechanism is still unclear. Here the effect of bFGF on endothelial cell expression of integrin beta 1 subunits was determined with Western Blot and image analysis method. The result reveal that the mean gray value of immunostain is 166.1 +/- 9.86 in the experimental group and 175.32 +/- 5.12 in the control group, suggest that bFGF may upregulate the endothelial cell expression of integrin beta 1 subunits. It is concluded that the bFGF may induce the overexpression of endothelial cell integrin beta 1 subunits to increase the cell adhesion onto polyurethane material. It is suggested that bFGF can be taken as an important assistant factor in accomplishment of endothelialization on artificial heart and blood vessel.
Animals ; Cell Adhesion ; drug effects ; Cells, Cultured ; Endothelium, Vascular ; cytology ; metabolism ; Fibroblast Growth Factor 2 ; pharmacology ; Integrin beta1 ; biosynthesis ; Polyurethanes ; Swine

The cultural model of porcine aortic endothelial cell (EC) was set up. By combined use of basic fibroblast growth factor(bFGF) with extracellular matrix (ECM), the endothelialization on PU under static culture was accomplished and the attachment rate as well as the morphological changes of EC under flowing shear stress condition were observed when LVAP was working at normal blood circulation pressure (12 KPa). The result revealed that EC treated with bFGF grew very well on the PU material precoated with fibronectin and collagen IV. Under shear stress condition for 1 and 2 hours, the percentage of cell attachment attained 74.42% and 59.7%. The general morphological changes of the cell were not obvious under the scanning electron microscope, slight shrink and cell death or loss were found. This work was done to find out the condition and method of experiment for the accomplishment of endothelialization under the flowing shear stress in the cavity of LVAP.
Animals ; Aorta ; cytology ; Cell Adhesion ; Cells, Cultured ; Endothelium, Vascular ; cytology ; Extracellular Matrix ; metabolism ; Fibroblast Growth Factor 2 ; pharmacology ; Heart-Assist Devices ; Polyurethanes ; Shear Strength ; Swine