1.Adverse Reaction of Netilmicin
Min LONG ; Ying WANG ; Rong CHEN ;
China Pharmacy 2001;0(12):-
OBJECTIVE:To explore the relevant factors that led to the adverse drug reaction(ADR)of netilmicin.METHODS:ADR cases resulted from netilmicin reported in12kinds of Chinese periodicals from1998to2003were analyzed statistically.RESULTS:The ADR of netilmicin involved multiple organs and systems,the clinical manifestation were compli-cated and diversified,which manifested mainly on the renal toxicity,toxicity of nervous system and allergic reaction.CON-CLUSION:Clinician and pharmacists should be alert to the ADR of netilmicin and keep using drugs rationally.
2.Determination of tauroursodeoxycholic acid in compound bile capsule by HPLC.
Fei CHEN ; Hai-Lin LONG ; Hai-Min LEI ; Qiang LI
China Journal of Chinese Materia Medica 2014;39(5):838-840
OBJECTIVETo discriminate and determine of the artificial bear bile of the compound bile capsule.
METHODTaking the pharmacopoeia as reference, the artificial bear bile was discriminated and determined by HPLC.
RESULTThe compound bile capsule and the control sample had chromatographic peak at the same time from HPLC. The content of the artificial bear bile was above 10 mg per tablets.
CONCLUSIONThe artificial bear bile of compound bile capsules can be discriminated effectively and determined accurately by HPLC method.
Animals ; Bile ; chemistry ; Capsules ; analysis ; Chromatography, High Pressure Liquid ; methods ; Discriminant Analysis ; Medicine, Chinese Traditional ; Taurochenodeoxycholic Acid ; analysis ; Ursidae
3.Cloning and Expression of Brevibacterium sp. DGCDC-82 Cholesterol Oxidase Gene in Escherichia coli
Long-Gang WANG ; Min-Chen WU ; Wu WANG ;
Microbiology 1992;0(06):-
Based on DNA sequence encoding cholesterol oxidase reported on the NCBI,cholesterol oxidase gene was cloned from Brevibacterium sp. DGCDC-82 by PCR methods,which showed homology of 98% to the previously reported cholesterol oxidase gene from Brevibacterium sterolicum ATCC21387. Subsequently,the resulting products were digested with NcoI and EcoRI and ligated to the pET28a vector by T4 DNA ligase.The recombinant plasmid,pET28a-choB,was transformed into Escheriehia coli BL21-CodonPlus(DE3)-RP which contain extra copies of the argU and proL genes.The positive clone was induced with IPTG,and enzyme expressed in BL21-CodonPlus(DE3)-RP,the enzyme activity was about 340U/L.The expression products were analyzed by SDS-polyacrylamide gel electrophoresis indicating that about 55kD protein was obtained,which accounted for about 16% of the total cell protein.
4.Analysis difference of lymphocyte subgroups between children with severe pneumonia and non-severe pneumonia
Siqi LONG ; Li MA ; Min CHEN ; Hong LI
Chinese Journal of Immunology 2016;32(3):401-404
Objective:To observe the levels of lymphocyte subgroups in children with severe pneumonia and non -severe pneumonia , and to investigate the change of celluer immune funciton of patients , moreover provide available reference for treatment.Methods:Flow cytometry instrument was used to detect peripheral blood CD 3+T, CD3+CD4+T, CD3+CD8+T, CD19+B lymphocytes and NK cells in 27 children with severe pneumonia and 28 children with non-severe pneumonia.In addition 20 heathy children were selected as the controls .Results:Compared with the heathy control group ,the levels of CD3+T,CD3+CD4+T,CD3+CD8+T lymphocytes and NK cells in children with severe pneumonia decreased significantly (P<0.01,P<0.05,P<0.01,P<0.05),while CD4/CD8 ratio,CD19+B cells were significantly increased (P<0.05,P<0.01).The level of CD3+CD8+T lymphocytes in children with non-severe pneumonia significantly lower than the heathy control group (P<0.01),but the level of CD19+B cells in children with non-severe pneumonia significantly higher than the heathy control group (P<0.01).Compared with non-severe pneumonia group.The levels of CD3+and CD3+CD8+T lymphocytes in children with severe pneumonia decreased significantly (P<0.01),however CD19+B cells were significantly increased ( P<0.01 ) .Conclusion: Immune dysfunction exists in both of children with severe pneumonia and non-severe pneumonia.Children with severe pneumonia has more serious damage in inmmunity .This provides certain theoretical basis for clinical e-valuation and treatment .
5.Resilience and influencing factors of kidney dialysis patients
Junrun XIE ; Long CHEN ; Min LI ; Hong LIU
Chinese Journal of Medical Education Research 2014;(8):826-829,830
Objective To investigate renal dialysis patients' resilience and influencing factors for clinical teaching. Methods Connor-Davidson resilience scale(CD-RISC),simplified coping style questionnaire (SCSQ), perceived social support scale (PSSS) and positive and negative affect scale (PANAS) were used for a survey of 160 renal dialysis patients in Xinan Hospital and Xinqiao Hospital (156 valid questionnaires) and the questionnaire information was put into the computer by the two-input mode and was made the questionnaire data analysis, single factor analysis and variance and re-gression analysis of by SPSS 18.0. Results Renal dialysis patients' resilience has no significant differ-ence in gender, frequency of kidney dialysis and kidney dialysis time (P>0.05), but resilience and positive coping scores of the age group between 31 to 40 were significantly higher than those of the group between 41-50 group(P=0.000). Taking the education background into consideration, resilience and positive coping scores of junior high school or above education were significantly higher than those of primary school education (P=0.000,P=0.000). Positive emotion, positive coping, and social support were positively correlated with resilience and negative emotions were negatively correlated with resilience in linear regression analysis. Conclusion Kidney dialysis patients who are over the age of 40 and less educated may have lower resilience, and less positive coping, more prone to nega-tive emotions, which is not conducive to the rehabilitation and treatment of disease. For kidney dialysis patients, improving their positive response, perceived social support and positive mood, and reducing their negative emotions are beneficial to the improvement of their resilience.
6.Relationships among posttraumatic growth, social support, and coping style in women with infertility
Yongju YU ; Li PENG ; Long CHEN ; Ling LONG ; Wei HE ; Jing LI ; Min LI
Chinese Journal of Behavioral Medicine and Brain Science 2013;22(8):707-709
Objective To explore the relationships among posttraumatic growth (PTG),social support,and coping style in women with infertility.Methods 182 women with infertility were recruited in one public hospital department of assisted reproduction technology.Measures included Posttraumatic Growth Inventory (PTGI),Perceived Social Support Scale (PSSS),and Simplified Coping Style Questionnaire (SCQ).Results ① The PTG score of women with infertility was 42.55 ± 16.83,lower than the average level.② Compared with the lower PTG group,the higher PTG group scored significantly higher in social support,positive coping,and negative coping (t =3.867,P< 0.01 ; t =8.452,P< 0.01 ; t =2.817,P< 0.01).③ There were significantly positive correlations among PTG,social support and positive coping (r =0.295-0.515,P < 0.01).④ Positive coping served as a total mediator in the relationship between social support and PTG.Conclusion There are closely correlations among PTG,social support,and positive coping style.Positive coping style significantly mediate the relationship between social support and PTG.
7.Effect of citric acid stimulation on salivary alpha-amylase, total protein, salivary flow rate and pH value in Pi deficiency children.
Ze-min YANG ; Long-hui CHEN ; Jing LIN ; Min ZHANG ; Xiao-rong YANG ; Wei-wen CHEN
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(2):188-192
OBJECTIVETo compare the effect of citric acid stimulation on salivary alpha-amylase (sAA), total protein (TP), salivary flow rate, and pH value between Pi deficiency (PD) children and healthy children, thereby providing evidence for Pi controlling saliva theory.
METHODSTwenty PD children were recruited, and 29 healthy children were also recruited at the same time. Saliva samples from all subjects were collected before and after citric acid stimulation. The sAA activity and amount, TP contents, salivary flow rate, and pH value were determined and compared.
RESULTS(1) Citric acid stimulation was able to significantly increase salivary flow rate, pH value, sAA activities, sAA specific activity and sAA amount (including glycosylated and non-glycosylated sAA amount) in healthy children (P<0.05), while it could markedly increase salivary flow rate, pH value, and glycosylated sAA levels in PD children (P<0.05); (2) Although there was no statistical difference in determined salivary indices between the two groups (P>0.05), salivary indices except salivary flow rate and glycosylated sAA levels decreased more in PD children. There was statistical difference in sAA activity ratio, sAA specific activity ratio, and the ratio of glycosylated sAA levels between PD children and healthy children (P<0.05).
CONCLUSIONPD children had decreased response to citric acid stimulation.
Child ; Citric Acid ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Saliva ; Salivary alpha-Amylases ; metabolism ; alpha-Amylases
8.Fermentation of Bacillus subtilis ge25 strain and preliminary study on its antagonistic substances.
Chen-Yun HU ; Yong LI ; Min LIU ; Wan-Long DING ; Min-Jian QIN
China Journal of Chinese Materia Medica 2014;39(14):2624-2628
Panax ginseng is one of the most important traditional Chinese herbal medicine, soil borne diseases influenced the yield and quality severely. In our previous work, endophytic Bacillus subtilis ge25 strain was isolated from ginseng root, and which showed significant antagonistic activity against several most destructive ginseng phytopathogens. In the present work, crude protein and lipopeptid extracts were prepared from LB and Landy supernate by salting out, acid precipitation methods respectively. The antagonistic activity of crude extracts and stability to temperature and protease digestion were examined by ginseng phytopathogen Alternaria panax. Results showed that, the antagonistic activity of crude protein extracts from LB culture was complete and partially lost when treated by high temperature and proteinase K. However, crude lipopeptid from Landy culture showed significant stabile antagonistic activity to them. Acid-hydrolyzation and TLC-bioautography analysis showed, that the crude lipopeptide contained at least one cyclic lipopeptide. In consideration of the stability and perfect antagonistic activity of ge25, further researches will promote the biocontrol of ginseng diseases in the field.
Alternaria
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drug effects
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physiology
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Bacillus subtilis
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metabolism
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physiology
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Bacterial Proteins
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isolation & purification
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metabolism
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pharmacology
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Endopeptidase K
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metabolism
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Endophytes
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metabolism
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physiology
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Fermentation
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Lipopeptides
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isolation & purification
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pharmacology
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Panax
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microbiology
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Plant Roots
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microbiology
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Temperature
9.Influence of oxidative/antioxidative biomarkers and inflammatory cytokines on rats after sub-acute orally administration of titanium dioxide nanoparticles.
Di ZHOU ; Zhang Jian CHEN ; Gui Ping HU ; Teng Long YAN ; Chang Mao LONG ; Hui Min FENG ; Guang JIA
Journal of Peking University(Health Sciences) 2020;52(5):821-827
OBJECTIVE:
To evaluate the sub-acute oral effect of titanium dioxide (TiO2) nanoparticles on the oxidation/antioxidation biomarkers and inflammatory cytokines in blood, liver, intestine, and colon in rats.
METHODS:
Twenty four 4-week-old clean-grade Sprague Dawley (SD) rats were randomly devided into 4 groups by body weight (n=6, control, low, middle, and high), in which the rats were orally exposed to TiO2 nanoparticles at doses of 0, 2, 10 and 50 mg/kg body weight/day for 28 consecutive days separately. Food intake, body weight and abnormal behaviors during the experiment were recorded. The rats were euthanized on the 29th day. The blood was collected via abdominal aortic method and centrifuged to collect the serum. Tissues from liver, intestine and colon were collected and homogenated. Then enzyme-linked immunosorbent assay (ELISA) and microwell plate methods were used to detect oxidation/antioxidation biomarkers including superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-Px), total mercapto (T-SH), glutathione disulfide (GSSG), malomdialdehvde (MDA) and inflammatory cytokines including interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in the serum, liver, intestine and colon in the rats.
RESULTS:
Compared with the control group, no significant differences in body weight, food intake and organ coefficients were observed in all the three groups after TiO2 gavage. No significant changes in GSH, GSH-Px, T-SH, and IL-6 were observed. Compared with the control group, significant increase of SOD activity in serum in high dose group, signi-ficant increase of GSSG concentration in intestine in middle and high dose group and significant increase of MDA concentration in liver in low and high dose group were observed. Compared with the control group, a significant increase of TNF-α in liver in middle and high dose group was observed.
CONCLUSION
TiO2 nanoparticle can increase antioxidant enzymes activities in blood, increase oxidative biomarkers in liver and intestine, increase inflammatory cytokines in liver in rats after a 28-day sub-acute orally administration. Among blood, liver, intestine, and colon, liver is most sensitive to the toxicity induced by TiO2 nanoparticles, followed by intestine, blood, and colon in sequence.
Animals
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Antioxidants
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Biomarkers
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Cytokines
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Nanoparticles
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Oxidative Stress
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Rats
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Rats, Sprague-Dawley
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Titanium
10.Application of mouse anti AEG-1 monoclonal fluorescent antibody in malignant serous cavity effusions
Man LU ; Min LONG ; Chong LIU ; Xi CHEN ; Chen WANG ; Huizhong ZHANG
International Journal of Laboratory Medicine 2016;(3):289-290
Objective To use mouse astrocytes elevated gene‐1 (AEG‐1) monoclonal fluorescent antibody for detecting tumor cells in malignant serous cavity effusions .Methods The expression of AEG‐1 in serous cavity effusion exfoliated cells by PCR and Western‐blot ;the mouse monoclonal anti‐AEG‐1 fluorescent antibody and tumor cells in malignant serous cavity effusions were co ‐incubated ,meanwhile ,the serous cavity effusions in benign lesions were taken as the negative control .Results The AEG‐1 expres‐sion was positive in malignant serous cavity effusions exfoliated cells ,while which in benign lesion serous cavity effusion was nega‐tive or weakly positive ;meanwhile the results of direct labelling in mouse anti AEG‐1 monoclonal antibodies were consistent with the results by PCR and Western‐blot .Conclusion Mouse anti AEG‐1 monoclonal fluorescence antibody can provide certain theoret‐ical basis for the detection of tumor cells in serous cavity effusion .