Objective To study the influence of microenvironment created by dissoluble cytokines derived from hepatocellular carcinoma cells (HCCs) on the functional status of mitochondria in dendritic cells (DCs) in order to investigate the mechanisms of tumor immune escape.Methods CD14+ monocytes,which were isolated with immune magnetic beads from fresh peripheral blood of healthy human,were then cultured in RPMI1640/10% FBS supplemented with 100 ng/ml rhGM-CSF and 100 ng/ml rhIL-4 for 7 d to develop into immature DCs (imDCs).Maturation was induced by addition of 20 ng/ml rhTNF-? to imDCs for another 3 d of culture.Acquired imDCs and mDCs were respectively co-cultured with human HCCs for 48 h in Transwell chamber.These treated cells were investigated for mitochondrial membrane potential,enzyme activity and intracellular Ca2+ concentration ([Ca2+]in) in the cytoplasma by immune fluorescence and MTT assay.Those untreated cells served as control.Results After co-cultured with HCCs,flow cytometry showed that the mitochondrial membrane potentials of imDCs and mDCs were respectively decreased from (659.991?17.052)and(473.741?11.676)to(482.681?7.935)and(407.189?5.051)(P

Objective To study the influence of microenvironment created by dissoluble cytokines derived from hepatocellular carcinoma cells (HCCs) on the expression of IL-12 of dendritic cells (DCs) at different differentiating stage. Methods The CD14+ monocytes,isolated with immune magnetic beads from fresh peripheral blood of healthy human,were cultured in RPMI 1640/10% FBS supplemented with 100 ng/ml rhGM-CSF and 100 ng/ml rhIL-4 for 7 d to develop into immature DCs (imDCs). Maturation was induced by addition of 20 ng/ml rhTNF-? to imDCs for another 3 days' culture. Acquired imDCs and mDCs were respectively co-cultured with human HCC Bel7402 cells for 48 h in Transwell chamber. The concentrations of IL-10,IL-12,TGF-?1 and VEGF in culture supernatant were investigated by ELISA,imDCs and mDCs cultured alone served as control. Results After co-cultured with Bel7402 cells,IL-12 were decreased from (115.076?8.129) pg/ml to (17.599?1.757) pg/ml in imDCs,and from (258.346?3.609) pg/ml to (6.787?1.123) pg/ml in mDCs (P

A method for the quantitative determination of tetrandrine in "Zhitong Ruanjiaonang"wasdeveloped by means of TLC densitometry. The method is easy, rapid, accurate, and repro-ducible . The average recovery was 99.98%.

OBJECTIVE:To prepare compound alendronate sodium sustained-release tablet and to investigate its drug release profile in vitro. METHODS: Hydroxy propyl methylcellulose (HPMC), ethylcellulose (EC), and lactis anhydrous were used to prepare sustained-release tablets by wet granule compression technique with release rate in vitro as index. Orthogonal experiment was conducted to optimize the formula. The release rate of the tablets in vitro was investigated. RESULTS: The optimized formula was as follows: HPMC 80 mg, EC 20 mg, and lactis anhydrous 20 mg. 12 h drug release of the preparation was achieved, and the drug release behavior in vitro fitted Higuchi equation. CONCLUSION: The sustained-release tablet is reasonable in formula and satisfactory in slow release efficacy.

Objective To investigate the association between a genetic variant in Dicer and cancer risk .Methods Both English and Chi-nese databases were carefully searched comprehensively .Based on the including and excluding criteria ,literatures that were eligible were carefully screened and data were retrieved .Meta analysis was performed by RevMan 5 .1 software .Pooled odds ratios(OR) and 95% confi-dential interval(CI)were used for analysis .Results Ten eligible literatures were included in the meta analysis .Meta analysis showed that the mutant allele C in 3′untranslated region polymorphism loci rs1057035 of Dicer gene might reduce the cancer risk(OR=0 .92 ,95% CI=0 .87-0 .97 ,P=0 .003) .Conclusion The variant rs1057035 T>C in Dicer gene could be related to cancer susceptibility .

Objective To investigate the association between the genetic variant in the promoter region of miR-34b/c and cancer risk.Methods Databases such as PubMed、EMBase、Wanfang、VIP、CNKI and so on were searched comprehensively.Based on the including and excluding criteria,literatures that were eligible were screened and data were retrieved.Meta analysis was performed by RevMan 5.1 software.Results Through searching and manually searching relevant references,a total of 9 articles with 10 independent studies were included.No significant associations were detected in C vs T,CC vs TT,CC + CT vs TT and CC vs CT + TT comparison models.However,in the CT vs TT comparison model,the result showed a significant association (Z =2.33,P =0.02).Meanwhile,subgroup analysis of hepatocellular carcinoma and colorectal cancer both showed significant associations with the polymorphism,with C vs T (OR =1.11,Z =2.10,P =0.04),CT vs TT (Z=2.40,P =0.02),CC +CT vs TT (Z=2.45,P=0.01) and CC vs TT (OR =0.66,Z=2.43,P=0.02),CC vs CT + TT (OR =0.67,Z =2.40,P =0.02) respectively.The C allele increased significantly the susceptibility of hepatocellular carcinoma,and the CC genotype reduced significantly the susceptibility of colorectal cancer.Conclusion The genetic variant in the promoter region of miR-34b/c rs4938723 T ＞ C is significantly associated with the susceptibility of hepatocellular carcinoma and colorectal cancer.

Aged ; Aged, 80 and over ; Female ; Humans ; Hyponatremia ; chemically induced ; Iodipamide ; adverse effects

AIM:To develop a microbiological method for determining plasma doxycyclineMETHODS:Plasma concentration of doxycycline was detected with Staphylococcus aureus ATCC 26003 and Micrococcus luteus ATCC 28001 strains in a variety of culture media and pHsRESULTS:Linear scope of doxycycline(016～100mg/L,r=09 985) could be achieved with using a microbiological method,which consisted of staphylococcus aureus and bovine heart soup culture medium at pH 50CONCLU_SION:This method can be used in study of pharmacokinetics and bioavailability of doxycycline

Objective To study the molecular mechanism of activator protein (AP)-1 and macrophage inflammatory protein (MIP)-1? in adjuvant arthritis and the effects of sodium arsenite (SA) on AP-1 and MIP-1. Methods Forty Wistar female rats were randomly divided into 4 groups: normal control (NC), model (M), low concentration sodium arsenite (SA1) and high concentration sodium arsenite group (SA2). The SA1 group and the SA2 group were treated with sodium arsenite (0.5 mg?kg-1?d-1 and 1.0 mg?kg-1?d-1) through abdominal cavity injection for 20 days, the normal control group and the model group were treated with saline (0.2 ml/d). The levels of C reactive protein (CRP) in every group were determined by biochemistry, the C-fos and MIP-1? expression of synovium in 4 groups were determined by immunohistochemistry. Results The levels of CRP in the M group were increased more than NC group (P

Adolescent ; Adult ; Age Factors ; Aged ; Aged, 80 and over ; Body Weight ; Humans ; Laryngeal Muscles ; anatomy & histology ; Magnetic Resonance Imaging ; Middle Aged ; Palate, Soft ; anatomy & histology ; Pharynx ; anatomy & histology ; Sleep Apnea, Obstructive ; pathology ; Tongue ; anatomy & histology ; Young Adult