Recent studies have revealed that obease increases the risk of differentated thyroid carcino-ma (DTC);iodine increases the risk of papillary thyroid carcinoma wheras decreases the risk of follicular thy-roid carcinoma;vitamin D decreases the risk of DTC,but there are not enough evidences to prove that vitamin A,C and E can decrease the risk of DTC;alcohol may decrease the risk of DTC,but the mechanism is still un-known;xenobiotics increases the risk of DTC.It is essential for DTC prevention to clarify the protection and risk factors.

Objective To investigate the effects of the different concentration of mineral trioxide aggregate (MTA) on the proliferation and differentiation of dental pulp stem cells (DPSCs) from the young permanent teeth.Methods DPSCs were isolated from the young permanent teeth and cultured by tissue explant method.The expression of STRO-1 was detected by using immunofluorescence technology.DPSCs were cultured with different concentrations of MTA (0.02,0.20,2.00,20.00 g/L).Cell proliferation was detected by MTT array.Cells were cultured in the appropriate concentration of MTA for 4 weeks,and then stained by Alizarin red to detect their mineralized nodule formation capacity.The cells were cultured with the appropriate concentration of MTA and collected after 12,24,36,48 h.The mRNA expression of ALP,BSP,OC and DSP after the treatment of MTA were detected by quantitative PCR.Results DPSCs were positive for STRO-1.The capacity of 0.20 g/L MTA promoting the proliferation of DPSCs was stronger than other concentrations.After 4 weeks,the mineralized nodules of DPSCs were observed after alizarin red staining.The PCR showed that with increasing induction time,the expression levels of DSP and OC were up-regulated.But that of ALP and BSP was increased first and then decreased.Conclusions In this study,MTA can promote the proliferation of DPSCs at 0.02,0.20,2.00 g/L concentration.It can induce odontoblast differentiation effectively by 0.20 g/L MTA.

Objective: In order to implement effectively on-scene medical response to a nuclear or radiological emergency, and also keep track of relevant on-scene audio and video information and monitoring data, we have designed and developed a monitoring platform for medical response to nuclear or radiological emergency. Methods:Based on relevant national standards for emergency response and the features of nuclear or radiological emergency, exploiting modern information technology and monitoring equipments, we formulate the design idea, characteristics, functions and framework of the platform. This platform takes treatment centers for nuclear or radiological injuries, designated medical institutions and medical response teams as main users. Results:We have developed the monitoring platform for medical response to nuclear or radiological emergency. Conclusion:Through the application of this platform, we hope to enhance the preparedness and on-scene response capability for nuclear or radiological emergency, and also provide information support for disposal of nuclear or radiological emergency in the future.

OBJECTIVE:To establish a method for the contents determination of 4 amino acids in Pyrrosia lingua. METHODS:The samples were analyzed by RP-HPLC. It was performed on a column of Welchrom C18 with the mobile phase A of 0.1 mol/L So-dium acetate buffer(pH6.5)- acetonitrile(93∶7,V/V)and mobile phase B of acetonitrile-water(4∶1,V/V)(gradient elution)at the flow rate of 1.0 ml/min,detection wavelength was 254 nm,column temperature was 30 ℃ and the sample size was 5 μl. RE-SULTS:The linear range was 4.92-49.2 μg/ml for glutamate(r=0.999 1),3.168-31.68 μg/ml for glycine(r=0.999 9),2.6-26 μg/ml for alanine(r=0.999 4)and 2.88-28.8 μg/ml for leucine(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 2.2%;average recoveries were 98.4%(RSD=2.2%,n=9),99.6%(RSD=2.5%,n=9),98.3%(RSD=2.2%,n=9)and 100.1%(RSD=2.3%,n=9),respectively. CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the contents determination of 4 amino acids in P. lingua.

AIM: To investigate the clinic efficacy and operation safety of improved small incision extracapsular cataract extraction in the sight-regaining program.
METHODS: Totally 82 cases ( 82 eyes ) included in the sight-regaining program of the cataract patients were underwent small incision extracapsular cataract extraction conbined with improved technology ( anterior capsule staining, continuous curvilinear capsulorhexis, auxiliary incision ) , postoperative visual recovery and operative complications were observed.
RESULTS: Postoperative visual acuity: uncorrected visual acuity when out of hospital: ≥0. 3 were 67 cases (82%);1wk after dismissed from hospital:≥0. 3 were 76 cases (93%), admist which ≥0. 8 were 45 cases (55%). There is no serious complication such as posterior capsule rupture, zonular dialysis, etc. in the operation.CONCLUSION: Combined use of improved technology can increase operation safety of small incision extracapsular cataract extraction.

Choroidal metastasis is one of the most common malignant tumors inside the eyes. It causes pain, hypopsia and some other related symptoms. It reduces the quality of the patients' life. It's significant for the patients to be detected and treated early, therefore they will have better vision and longer life. The treatments of choroidal metastasis are developing quickly. Both the vitreous cavity injection of targeted drug and gene therapy are hot topics of research. This paper summarizes the etiology, development, diagnosis and treatment of choroidal metastasis nowadays.

[Objective]To investigate the effect of transforming growth factor ?1(TGF-?1) genes transfection on the proliferation and differentiation of chondrocyte by adenovirus vector,and to observe the quality in repair cartilage defect through constructing tissue-engineered cartilage.[Method]Using replicat ion defective adenovirus Adeno-X~(TM) as a carrier,Articular chondrocytes were transferred by high titers level recombinant adenovirus taking TGF-?1 genes.Ultromicrostructure,prolife ration anddifferentiation were observed by light and electron microscope,flow cytometry and so on.Induce expression of exogenous genes coding protein and cartilage cell phenotype were detected by Immunocytochemistry and Northern blot.Gross observation,histology and effect classification were used to evaluate results in repair cartilage defect by combi nation of infected chondrocytes and bone matrix gelatin(BMG).[Result]Immunocytochemistric staining showedthe expression of exogenous gene coding proteins after infection,and procollagen Ⅱ mRNA in cells was detected increasingly by Northern blot.TGF-?1 stimulated proliferation of chondrocytes in primary cultured,and resulted in recruitment of articular chondrocytes into S-G2/M phase.Chondrocytes seeded onto BMG showed high level of proliferation.Transplantation in vivo showed: cartilage defect was repaired satisfactorily by Combination of BMG and infected chondrocytes,and the structure of repair tissue got close to normal articular cartilage.[Conclusion] Articular chondrocytes prolifera tion could be stimulated by adenovirus vector taking TGF-?1 genes,which could be used to constructed tissue-engineered cartilage,and applied in repairing joint cartilage defect.

0.10). Conclusion: Donepezil is effective in treating the cognition of AD and some of the mixed dementia, but it has little effect on VD.

Objective To study the effect of rehabil it ation training on the expression of S-100, GFAP and Nestin in the cortex of cer ebrally infarcted rats. Methods Sixty Sprague-Dawle y rats were used. Experimental cerebral infarction was caused in all the rat s. 24 hours after the infarction, all the rats were randomly divided into 3 grou ps: a rehabilitation group, an immobiliaztion group and a control group. The rat s in the rehabilitation group were given balancing, grasping, rotating and walki ng exercises everyday, while those in the immobilization group were immobilized in cages. The rats in the control group was without any treatment. Immunohistoc hemical technique was used to detect the S-100, GFAP and Nestin expression at t he time points of 24 hours, 1, 2, 3 and 4 weeks after infarction, respectively. Results S-100, GFAP and Nestin expression were foun d around the infarcted cortex. The response increased as the extension of the su rviving time. The immunohistochemical reaction in rats in the rehabilitation gro up was stronger than that in the immobilization group. Conclusi on Rehabilitation training can activate astrocytes, which could impr ove the internal environment, protect the neurons and promote repairing of neuro n.

Objective: To discuss the purposes and methods of determining and employing operational intervention levels in preparedness and response for a nuclear emergency. Methods:According to the safety standards and technical reports issued by the International Atomic Energy Agency (IAEA) and combined with specific conditions in China, the operational intervention levels suitable for applying in China are analyzed. Results: The newly published IAEA technical report provided special operational intervention levels for emergency at a light water reactor. The recommended values are practical that our country can use for reference. Conclusion: It is suggested that the related branches for response to nuclear emergency in China establish feasible operational intervention levels by referring to the IAEA reports and taking into account the special conditions of nuclear facilities in China, in order to improve the capability of medical response to nuclear emergency.