Brain Abscess ; etiology ; Female ; Humans ; Leuconostoc ; pathogenicity ; Male ; Sepsis ; complications ; microbiology

Objective: To investigate the expression of molecular marker ABCG2 of stem cells and tumor angiogenesis-related gene products including vascular endothelial growth factor (VEGF), CD34 and very late antigen alfa-2 (VLA-2α) in human glioma-bearing nude mice and observe the patterns of angiogenesis in transplanted tumors by CD34-PAS double staining. Methods: The human glioma cells were subcutaneously transplanted in 30 nude mice. Six normal brain tissues were as controls. The hematoxylin and eosin staining and immunohistochemical examination were performed to determine the positive rate and the hot-spot of gene product expression. The patterns of angiogenesis were observed under light microscope. Results: The positive rates of ABCG2, VEGF, and VLA-2α expression were all 100% in transplanted tumors. The average percentage of positive cells was (3. 93 ± 1. 438)%, (34. 84 ± 6.212)% (30.33 ± 4.428)%, respectively. The value of micro-vascular density (MVD) labeled by CD34 averaged at 29. 78 ± 5.88. All the three microcirculation patterns, endothelium-dependent vessels, mosaic vessels and vasculogenic mimicry, were present in xenografted human glioma tissue samples. Conclusions: The ABCG2-overexpressed cells tended to be located close to blood vessels. VEGF and VLA-2α play an important role in angiogenesis of transplanted tumors in nude mice. There exist three different microcirculation patterns in high malignant transplanted tumors.

Objective To evaluate the feasibility of cell free fetal DNA(cffDNA)-based noninvasive prenatal diagnosis,we developed a precise technique for fetal sex determining region of Y chromosome(SRY)gene detection using size-fractionated cell-free DNA in maternal plasma.Methods Peripheral blood samples were collected form 117 pregnant women.cffDNA was extracted based on a column absorbent method and isolated by agarose gel electrophoresis.A dulex-polymerase chain reaction(PCR)was used to detected SRY gene and glycerol-dehyde-phosphate dehydrogenase(GAPDH)gene.Results Both SRY and GAPDH gene were detected in 86 cffDNA samples from women bearing male fetuses.And only GAPDH gene was detected in 71 cffDNA samples from women bearing female fetuses.These results had a coincidence whit those of villus or amniotic fluid samples.The specificity and sensitivity reached 100%(117/117)and 100%(66/66),respectively.Conclusion By agarose gel electrophoresis,re-extratedand and dulex PCR,size-fractionated cell-free fetal DNA in maternal plasma can be selective enriched and used to noninvasive prenatal diagnosis of sex-linked disorders and single gene disorders.

Reactivation of hepatitis B virus is a common complication of patients with malignant tumor chemotherapy, and gets more attention in clinicians. But the mechanism of reactivation of hepatitis B virus is not completely understood. There is not a unified definition and the prevention and cure of reactivation of HBV at home and abroad. In this paper, the relevant content of HBV reactivation in recent years is reviewed.

Based on special biological characteristics and prognosis, the incidence of gastroesophageal junction adenocarcinoma (GEJA) has rapidly increased over recent years, and its diagnosis and treatment remain controversial. Compared with simple surgery, neo-adjuvant and adjuvant therapies can improve the survival of patients with locally advanced GEJA. Under neo-adjuvant therapy, neo-adjuvant chemoradiation is superior to neo-adjuvant chemotherapy alone for improving the resectability, maintaining loco-regional control, and improving the quality of life of the patient. The combination of this therapy with targeted drugs may further increase the ef-ficacy of GEJA. Most data on GEJA-treated patients were obtained from randomized clinical studies on esophageal cancer or gastric cancer. Thus, prospective randomized controlled studies with a large sample size should be performed to optimize the strategy of neo-adjuvant and adjuvant therapies, and further improve the treatment outcome. In this article, studies on the comprehensive treatment of GEJA were reviewed.

To measure carotid intima media thickness(IMT) and to investigate the relationship between carotid atherosclerosis and some major risk factors in elder uremic patients, a cross sectional study was carried out in 30unselected elder uremic patients (16on hemodialysis). Fasting blood sampling for serum lipids, BUN, creatinine, hemoglobin, and echo colour Doppler evaluation of common carotid arteries and heart were performed , BP was measured in all patients (before dialysis day in hemodialysis patients). Relationship between the results and miltifactoral regression analysis were also carried out. 17 patients(56 6%)had carotid IMTwhile 12 dialysis patients(75%) had it. 6patients (20%) had at least one plaque. A significant correlation was found in internal diameter of carotid arteries, IMT and blood vessel resistance between left and right carotid arteries. Carotid IMT had a close relationship with serum BUN, creatinine, cholesterol, systolic BP and heart ejection fraction. In multiple regression models, serum creatinine and cholesterol was significant and an independent predictor of the degree of carotid IMT. In elder uremic patients, carotid IMT is quite common. Elder age, hypertension, degree of renal insufficiency and dyslipidemia are associated with carotid atherosclerosis. Serum creatinine and cholesterol appears to serve as an independent predictor of carotid atherosclerosis, which contributes to the cardiovascular complications and high mortality in elder uremic patients.

Objective To study the effects of acute cadmium exposure on the immune function of white blood cells (WBC), plasma interleukin-2 (IL-2) and cortisol levels in rats. Methods Thirty-six male rats were randomly divided into three groups averagely. The control group (group C) and two experimental groups(groupⅠand group Ⅱ) were respectively exposed to 0, 0.5, 1.0 mg / kg body weight (BW) cadmium for 7 days by intraperitoneal injection. Blood samples of the rats were collected on the 4th and 7th day after administration of cadmium respectively and the related parameters were analyzed. Results The BW of rats in groupⅠand group Ⅱ were significantly lower than that of the group C. The WBC counts of two experimental groups were higher than that of the control group. Higher percentage of neutrophiles and lower percentage of lymphocytes were observed in rats of group Ⅰ and Ⅱ on the 7th day after cadmium exposure, while no obvious variations in monocytes(%), eosinophiles(%) and basophiles(%)were observed among the three groups. Blood T-lymphocyte(%) and IL-2 levels in rats of groupⅠandⅡdecreased on the 4th and 7th day after cadmium exposure respectively, while B-lymphocyte(%) increased on the contrary. Plasma cortisol levels in rats of groupⅠ and groupⅡ were higher than that of the group C on the 7th day after cadmium exposure. Conclusion The results showed that acute cadmium exposure could affect the WBC immune function and result in the defect of cellular immune function as well as significant change of adrenal cortex endocrine activities.

Objective To investigate whether diazoxide preconditioning can exert protective effect on myocardium against ischemia-reperfusion injury in immature rabbits and the possible mechanism. Methods Twenty-one healthy 3-4 week old white rabbits of either sex were randomly divided into 3 groups : group Ⅰ control ( n = 8) ; group Ⅱ diazoxide preconditioning ( n = 8) and group Ⅲ diazoxide + 5-HD preconditioning ( n = 5) . The animals were anesthetized with intraperitoneal pentobarbital 50 mg?kg-1 and heparized (150 IU?kg-1). The hearts were excised and connected to Langendorff apparatus and passively perfused with normothermic (37℃), oxygenated (95% O2 , 5% CO2) Krebs-Henseleit buffer (KHB) at a constant perfusion pressure of 70cmH2O. A latex balloon was inserted via left atrium into left ventricle and filled with water. The left ventricular end-diastolic pressure (LVEDP) was maintained at 10 mm Hg. In group I cardiac arrest was induced with St Thomas Ⅱ solution after the heart was perfused with KHB for 30 min. In group 11 after being perfused with KHB for 15 min, the hearts were perfused with diazoxide 100?mol?L-1 for 5 min followed by 10 min wash-out with KHB , then cardiac arrest was induced as in group Ⅰ . In group Ⅲ after being perfused with KHB for 15 min, the hearts were perfused with diazoxide 100?mol?L-1 and 5-HD 100?mol?L-1 for 5 min, followed by 10 min wash-out with KHB, then the cardiac arrest was induced as in group Ⅰ and Ⅱ . All hearts were subjected to 30 min ischemia followed by 45 min reperfusion after cardiac arrest. Coronary flow (CF), HR, left ventricular developed pressure (LVDP) and dp / dt max were measured after the hearts were perfused with KHB for 15 min (baseline) and at 5, 10, 15, 30, 45 min after reperfusion was resumed. Coronary effluent was collected at 5 min after reperfusion was resumed for determination of myocardial enzymes, CK, LDH and AST. At the end of experiment myocardial specimen was obtained for determination of ATP content and ultrastructure examination. Results There was no significant difference in the baseline hemodynamic parameters among the three groups. The rates of recovery of LVDP and ? dp / dt max after reperfusion were significantly higher in group Ⅱ than those in group I and Ⅲ ( P 0.05 ) , Conclusion Diazoxide can protect myocardium from ischemia-reperfusion injury by opening the mitochondrial KATP channel in immature rabbits.

Objective To investigate the treatment effect of recombinant bovine basic fibroblast growth factor (rb-bFGF) in patients with gingivitis and its influence on gingival sulcus bleeding index. Methods Sixty-eight patients with gingivitis treated in our hospital were randomly divided into control group (34 cases) and observation group (34 cases). The control group were given basic periodontal therapy, while the observation group were treated with rb-bFGF. The clinical effects, gingival sulcus bleeding index (SBI) before and after treatment, and the recurrence were compared between the two groups. Results After treatment, the total effective rate (97.06%) of the observation group was significantly higher than that of the control group (70.59%), the difference was statistically significant (P<0.05). The SBI of the observation group was significantly lower than that of the control group at 1 and 2 weeks after treatment (P<0.05). The recurrence rate of the observation group (5.88%) was significantly lower than that of the control group (23.53%) (P<0.05). The incidence of complications such as pain, periodontal abscess, bromopnea and loosening was significantly lower in the observation group (8.82%) than in the control group (32.35%). The difference was statistically significant (P<0.05). Conclusion After rb-bFGF treatment, the symptoms of toothache and gingival swelling disappear, normal function of teeth is restored, gingival sulcus bleeding improves significantly, with fewer adverse reactions and obvious clinical effect.