This paper introduces an intelligent vision analysis system for urinary sediments,which includes the function modules of mechanical,automatic adding sample and cleaning,images collecting,processing and intelligent vision recognition,system software and so on.It implements the course of adding sample,cleaning,images collecting,feature extraction,recognition and counting,self-learning automatically.

Objective To investigate the effect of propofol on the heme oxygenase-1(HO-1)expression in rats with H_2O_2-mediated damage to cultured cardiac myocytes.Methods Primary cultured new-born rat cardiac myocytes were divided into 8 groups:control,H_2O_2 group,low dose propofol (LP) group,middle dose propofol(MP)group,high dose propofol(HP)group,LP +ZnPP Ⅸ group.MP+ZnPP Ⅸ group and HP+ZnPP Ⅸ group.The cells in all groups were incubated for 6 h.The concentration of malondialdehyde(MDA),activity of superoxid dismuase(SOD),mitochondria and Caspase-3,and the expression of HO-1 were determined.Results As compared with H_2O_2 group,the HO-1 expression,and the activity of SOD and mitochondria were significantly increased(P<0.05,P<0.01),and activity of Caspase-3 and concentration of MDA was decreased(P<0.05)in MP and HP groups.As compared with LP,MP and HP groups respectively.the HO-1 expression and SOD activity were significantly decreased and the concentration of MDA increased in LP+ZnPP IX,MP+ZnPP Ⅸ and HP+ZnPP Ⅸ groups(P<0.05,P<0.01),while Caspase-3 activity was significantly increased in MP+ZnPP IX and HP+ZnPP IX(P<0.05,P<0.01).Conclusion Propofol can protect cardiac myocytes against H_2O_2-mediated cytotoxicity in a dose-dependent manner,and increase the HO-1 expression,which may partly mediate cytoprotective effects of propofol.

Objective To explore the means of treating postoperative gastrointestinal fistulas in patients with severe acute pancreatitis(SAP). Methods A retrospective study was carried out on the clinical data of 28 cases of postoperative gastrointestinal fistulas(PGF) in patients with severe acute pancreatitis admitted in our department between 1996 and 1999.Results The amount of drainage decreased significantly by using somatostatin(SS) and growth hormone(GH). All the patients were recovered, the average disappearred time of the fistula was (52?16) days(24～227 days).85.7% of the fistula were cured by non operative treatment.Conclusions Non-operative treatment is suitable to most of SAP patients with PGF. SS and GH can accelerate the healing of gastrointestinal fistulas.

Objective:TTo observe the clinical efficacy of noninvasive positive pressure ventilation(NIPPV)for AECOPD with respiratory failure and disturbance of consciousness.Methods:A total of 58 AECOPD patients with respiratory failure and consciousness in our hospital from 2011 July to August 2015 were randomly divided into NIPPV group and control group. The control group was received conventional therapy, and NIPPV was added to the NIPPV group. The heart rate(HR), respiratory rate(RR), Glasgow coma score(GCS) and blood gas analysis of patients before and after 24 h, 72 h of treatment, and the adverse reactions in NIPPV group were observed.Results: The PaCO2, RR, HR at 24 h and 72 h were significantly lower and PaO2 and GCS were significantly increased in NIPPV group. Compared to the control group, PaO2, PaCO2, HR and GCS after 24 h and 72 h were different (t=11.29,t=9.19,t=9.21,t=11.23,t=10.30,t=7.28, t=10.34,t=6.69;P<0.05) . NIPPV group had a higher cure rate and relatively low rate of endotracheal intubation than the control group. The differences are statistically significant(x2=10.07,x2=8.32,P<0.05). The hospital mortality is lower than the control group and there is no statistically significant(x2=3.50,P>0.05). Some patients in NIPPV group had initial discomfort, facial skin hyperemia erosion, mild gastrointestinal discomfort and fear, which were improved after symptomatic treatment.Conclusion: NIPPV treatment for AECOPD patients with respiratory failure and disturbance of consciousness which can effectively correct respiratory acidosis and CO2 retention. The spontaneous breathing and arterial oxygen level of patient can be gradually recovered, which are better than the conventional treatment.

Total pelvic floor reconstruction is a minimally invasive and effective method for the treatment of pelvic organ prolapsed. It is a minimally invasive and effective method for the treatment of pelvic organ prolapsed, and it significantly improves the quality of life of patients. However, due to the complex anatomy of the pelvic floor, the requirements of clinical physicians and nursing is high, and related complications are easy to occur. This article introduces the observation and nursing care of the complications such as the bladder/rectum and nerve injury, the erosion of the mesh, the symptoms of the pelvic floor reconstruction, the pain or discomfort of the intercourse. Summary of the recent 10 years research results, to provide reference and guidance for clinical nursing work.

Acute traumatic coagulopathy (ATC) attacks suddenly with a rapid progress and has an extremely highly mortality.Of late years more and more relative studies have made a great progress on the pathogenesis,diagnosis and treatment of ATC.However,there are currently no medical guidelines for ATC.There are still some problems such as nonstandard treatment,outdated concept and so on in clinic.Based on the latest research progresses home and abroad,this paper expounds emphatically the first-aid and treatment of ATC in order to bring helps to the clinical doctors.

Objective To observe the effects of neuregulin‐1 (NRG‐1)on calcium transients in mouse ventricular myo‐cytes.Methods Nine C57BL/6 mice of male were randomly dived into 3 groups :the blank control group ,NRG‐1 group ,and iso‐proterenol(ISO)group.Their hearts were removed and immediately cannulated via the aorta and retrogradely perfused with en‐zymatic isolation solution to get single ventricular cell by Langendorff system.Myocytes were loaded with the Ca2+ indicator Fluo‐4 and subjected to electrical field stimulation at 0.5 or 1 Hz by using living cells workstation.The change of fluorescene in‐tensity was recorded simultaneously in each group.Results Compared with the blank control group ,NRG‐1 group had signifi‐cantly increased Ca2+ transient amplitude ΔF/F0 (n=10 ,P<0.05) ,and reduced Ca2+ transient recovery time RT50 (n=10 ,P<0.05).There were no significant differences in Ca2+ transient amplitude ,Ca2+ transient recovery time and the time to peak be‐tween NRG‐1 and ISO groups(n=10 ,P>0.05).Conclusion NRG‐1 can increase Ca2+ transient amplitude and Ca2+ transients and reduce the time interval of Ca2+ transients.

OBJECTIVE To investigate the antibiotic resistance status in coagulase negative staphylococcus (CNS) strains and provide the scientific evidence for the reasonable use of antibiotics. METHODS The drug resistance of 251 strains of CNS was ananlyzed. RESULTS The detection rate of methicillin-susceptible coagulase-negative Staphylococcus(MSCNS) was 12.75% (32/251), while that of methicillin-resistant coagulase-negative Staphylococcus (MRCNS) was 87.25%. The drug-resistance rate of MRCNS was higher than that of MSCNS. Vancomycin resistant and intermediate strains had not been detected. CONCLUSIONS Laboratory should monitor the drug resistance well and update in time to offer the evidence for the reasonable use age of the antibiotics.

Objective: To compare the myocardial protection effect between off-pump coronary artery bypass(OPCAB)and on-pump coronary artery bypass. Methods: 70 patients were divided into 2 groups:off-pump coronary artery bypass(beating group,n=35), and cold blood cardioplegia solution group(cold group,n=35). There was no statistical difference in heart function, sex, age and weight before operation. Blood samples were taken for determine the serum concentration of creatine kinase MB isoenzyme (CK-MB) and troponin-T(cTnT) peri-operatively. Myocardial ultrastructure was also observed. Results: CK-MB, cTnT release in beating group during bypass and post-bypass was lower than that in cold group (P

Animals ; China ; epidemiology ; Disease Outbreaks ; prevention & control ; Endemic Diseases ; prevention & control ; Feces ; parasitology ; Humans ; Schistosomiasis ; epidemiology ; prevention & control