1.Compound polymyxin B ointment combined with desonide cream for the treatment of subacute or chronic ;eczema:a multicenter, randomized, double-blind, parallel-group, controlled clinical study
Xu CHEN ; Mei JU ; Chen YU ; Long GENG ; Junfan CHEN ; Ruohong LI ; Si LIANG ; Qinsi HUANG ; Gang WANG ; Xinghua GAO
Chinese Journal of Dermatology 2016;49(8):541-546
Objective To evaluate the clinical efficacy and safety of compound polymyxin B ointment combined with desonide cream for the treatment of subacute or chronic eczema. Methods A multicenter, randomized, double?blind, parallel?group, controlled clinical study was conducted. Totally, 144 patients with subacute eczema and 144 patients with chronic eczema were enrolled into this study, and both randomly and equally divided into the test group and control group. The test group and control group firstly topically applied compound polymyxin B ointment and its vehicle respectively, then both topically applied desonide cream 3 hours later. The drugs or vehicle were applied twice a day in all the patients. Patients′ symptoms and signs (including degree of itching, inflammation, erosion/exudation and infiltration/thickening, as well as area of target lesions) were evaluated, and the time to onset and duration of itching?alleviating effect were recorded. The clinical efficacy and safety of treatments were analyzed and compared between the test group and control group. Results The total symptom and sign scores significantly decreased to different extents on days 7 and 14 in the test group(subacute eczema patients:6.09 ± 2.78 and 3.68 ± 3.18 vs. 13.44 ± 1.66; chronic eczema patients: 6.56 ± 2.68 and 4.38 ± 3.27 vs. 12.96 ± 1.16)and control group(subacute eczema patients:8.26 ± 3.17 and 5.28 ± 4.05 vs. 13.60 ± 1.75;chronic eczema patients: 8.84 ± 2.90 and 6.25 ± 3.78 and vs. 12.64 ± 1.18)compared with those at baseline. Moreover, the total symptom and sign score of patients with subacute or chronic eczema was significantly lower in the test group than in the control group on days 7 and 14(all P<0.05). A significant increment was observed in the degree of decrease in scores for itch, infiltration/thickening in patients with subacute eczema in the test group compared with that in the control group(all P<0.01), as well as in scores for itch, infiltration/thickening and area of target lesions in patients with chronic eczema in the test group compared with those in the control group (all P < 0.05). In addition, patients with subacute eczema in the test group showed significantly shorter onset and longer duration of itching?alleviating effect than those in the control group(both P<0.05). The time to onset of itching?alleviating effect was also significantly shorter in patients with chronic eczema in the test group than in those in the control group(P<0.000 1), but there was no significant difference in the duration of it between the two groups of patients with chronic eczema. Clinicians and patients were both more satisfied with therapeutic effects in the test group than in the control group(all P<0.05). Conclusions Topical compound polymyxin B ointment can increase the efficacy of topical desonide cream for the treatment of subacute or chronic eczema, especially subacute eczema. Compound polymyxin B ointment also shows a favorable therapeutic effect on itching and infiltration/thickening in patients with eczema.
2.Application of transvaginal external fascia trachelectomy in the treatment of CIN and micro-invasive cervical cancer.
Si-yuan ZENG ; Mei-rong LIANG ; Long-yu LI ; Ling LI ; Wei JIANG ; Mei-ling ZHONG
Chinese Journal of Oncology 2013;35(7):543-546
OBJECTIVETo explore the feasibility and safety of transvaginal external fascia trachelectomy to conservatively treat patients with stage Ia1 squamous carcinoma of the uterine cervix (SCC) and cervical intraepithelial neoplasia (CIN) III, who are not suitable to take cold knife conization (CKC).
METHODSFrom July 2002 to September 2010, those patients who had a strong desire to preserve the uterus or fertility but also are confronted with following situations received transvaginal external fascia trachelectomy: CIN III with large area lesion (colposcopically observed lesion area was larger than 3/4 of the cervix), or patients with CIN II-III suffered recurrence or had persistent lesion or positive margin after CKC or LEEP, or patients with CIN II-III upgraded into stage Ia1 SCC through LEEP and pathological confirmation (except for those with lymphovascular space invasion), or CIN III patients complicated with upper vaginal intraepithelial neoplasia (VAIN). Their clinical information and data were reviewed and analyzed.
RESULTSAmong the 79 cases, who underwent transvaginal external fascia trachelectomy, six were stage Ia1 SCC, 61 were CIN III with a large area lesion (23 cases had glandular involvement), three were CIN III complicated with VAIN, six were CIN III with persistent lesion after LEEP, two were CIN III with positive margins after LEEP, and one case had recurrence after conization. The median age of these patients was 33 years old, ranging from 23 to 40 years old. The mean operation time was 39 min (rang 20-60 min), the average amount of bleeding was 40 ml (rang 1-300 ml) and the mean hospital stay was 10 d ( rang 6-17 d). The CIN III patients complicated with VAIN received this surgery with resection of the adjacent vaginal mucosa more than 2 cm in 3.8% (3/79). The median follow-up time was 49 months (8-85 months) and none of these patients had ureteral injury or large amount of intraoperative or postoperative bleeding or post-operative recurrence. No patient complained any effect on their sexual life. Among the five patients with reproductive desire, one was at her 22 w gestation after one induced abortion and one spontaneous abortion, four patients experienced term birth in which three were cesarean section and one was natural labour.
CONCLUSIONSTransvaginal external fascia trachelectomy is a safe and effective conservative treatment for stage Ia1 SCC, CIN III with large area lesion, CIN III complicated with VAIN and CIN II-III suffering recurrence, persistent lesion or positive margins after CKC and others that are not suitable to take CKC.
Adult ; Carcinoma, Squamous Cell ; pathology ; surgery ; Cervical Intraepithelial Neoplasia ; pathology ; surgery ; Cervix Uteri ; surgery ; Female ; Fertility Preservation ; Follow-Up Studies ; Gynecologic Surgical Procedures ; methods ; Humans ; Neoplasm Recurrence, Local ; surgery ; Neoplasm Staging ; Organ Sparing Treatments ; methods ; Uterine Cervical Neoplasms ; pathology ; surgery ; Young Adult
3.The analysis of hantavirus S gene in Apodemus agrarius in Changbai area.
Qing-Li YAN ; Peng-Feil YANG ; Li-Jun SHAO ; Yong-Xian LIU ; Yun PU ; Xiao-Long ZHANG ; Xiao-Mei CAO ; Tian-Yu GUO ; Li-Si YAO
Chinese Journal of Virology 2013;29(4):382-385
To gain more insights into epidemiologic characteristics and genotype of hantavirus in Apodemus agrarius in Changbai Area. Complete hantavirus S segment sequences were amplified by RT-PCR and sequenced. The phylogenetic trees were constructed for analysis of genetic characters of hantavirus. A total of 58 Apodemus agrarius were trapped in the epidemic areas, and complete hantavirus S segment sequences were obtained from 4 lung samples of these rodents (6. 90%0). Phylogenetic analysis of the four S segment sequences indicated that all viruses isolated from Apodemu sagrarius were closely related to genotype 6 of Hantaan virus (95. 8%-96. 3%, nucleotide identity; 98. 6%-99. 5%, amino acid identity), all of them had a specific S387 different from other genotypes of Hantaan virus.
Animals
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Base Sequence
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China
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epidemiology
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DNA, Complementary
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chemistry
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genetics
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Disease Reservoirs
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virology
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Genotype
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Hantavirus
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classification
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genetics
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isolation & purification
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Hantavirus Infections
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epidemiology
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veterinary
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virology
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Lung
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virology
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Murinae
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virology
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Phylogeny
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RNA, Viral
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genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Rodent Diseases
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virology
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Sequence Analysis, DNA
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Viral Proteins
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genetics
4.Effects of tanguticum maxim polysaccharide on ulcerative colitis induced by TNBS in rats.
Li LIU ; Qi-bing MEI ; Si-yuan ZHOU ; Feng-hua HAN ; Yin LONG ; Jia-yun LIU ; Chen LI ; Jing-ru MENG ; Zhi-peng WANG
China Journal of Chinese Materia Medica 2003;28(3):246-249
OBJECTIVETo evaluate the effects of Tanguticum Maxim polysaccharide (TMP-1) on TNBS-induced colitis in rats.
METHODRats with TNBS/ethanol-induced colitis were used and treated with TMP-1 and dexamethasone (DX). Seventy-two rats, including animals with TNBS-induced colitis, were treated with saline, TMP-1 (100, 200, 400 mg.kg-1) and DX. White blood cells were counted on the fifth day and the rats were killed by ether on the sixth day. SOD activity in serum, MPO and SOD activity of colonic tissue were measured.
RESULTThe remarkable effects of TMP-1 at dosage of 200, 400 mg.kg-1 on TNBS-induced colitis were observed. The ulcerative area was diminished and weight of colon was reduced. White blood cell population was reduced, SOD activity in serum and SOD activity of colon tissue were remarkably increased, and, MPO activity of colonic tissue was reduced.
CONCLUSIONTMP-1 has significant effects on TNBS-induced colitis in rats with lower side effects, which suggests the effective component of rhubarb on colitis perhaps is TMP. The mechanism of the actions of TMP may relate to its antiflammation, antioxidation and immunoloregulation.
Animals ; Anti-Inflammatory Agents, Non-Steroidal ; therapeutic use ; Colitis, Ulcerative ; chemically induced ; drug therapy ; Colon ; enzymology ; pathology ; Male ; Phytotherapy ; Plants, Medicinal ; chemistry ; Polysaccharides ; isolation & purification ; therapeutic use ; Rats ; Rats, Sprague-Dawley ; Rheum ; chemistry ; Trinitrobenzenesulfonic Acid
5.A comparative cytogenetic analysis in large scale between adult and childhood patients with acute lymphoblastic leukemia.
Xu-Ping LIU ; Xiao-Fan ZHU ; Jian-Xiang WANG ; Ying-Chang MI ; Yao ZOU ; Yu-Mei CHEN ; Cheng-Wen LI ; Yun DAI ; Shuang QIN ; Ji-Gang XIAO ; Fang-Yun XU ; Jin-Ying GONG ; Si-Ping WANG ; Cheng-Long YU ; Jing FAN
Journal of Experimental Hematology 2009;17(6):1399-1404
This study was purposed to comparatively analyze the cytogenetic characteristics between 566 cases of adult acute lymphoblastic leukemia (aALL) and 586 cases of childhood acute lymphoblastic leukemia (cALL). The cytogenetic analysis of all the patients was performed, and the FISH detection for partial patients was carried out. The result showed that the difference of chromosome abnormality between cALL and aALL was statistically significant. The percentage of abnormal karyotypes in aALL was 62.0%, including mainly t(9;22)(q34;q11), hypodiploidy, hyperdiploidy (47 - 50), abn(6q), abn(9p) and -7, most of which conferring an unfavorable prognosis. The percentage of abnormal karyotypes in cALL was 39.2%, composed mainly of high hyperdiploidy, hypodiploidy, TEL/AML1(+), +8, hyperdiploidy (47 - 50) and +21, etc, most of which conferring a favorable prognosis. The incidences of abnormal karyotypes, total hypodiploidy, total hyperdiploidy (47 - 50), t(9;22)(q34;q11), -7, abn(7q), abn(14q32) and +Ph in aALL were significantly higher than those of cALL (p < 0.05), whereas the incidences of normal karyotype (N), high hyperdiploidy, +8, +21*2 and TEL/AML1(+) in cALL were significantly higher than those of aALL (p < 0.05). 20.5% of aALL were Ph+ aALL, with 63.8% of which being with additional abnormalities, composed mainly of +Ph, -7, i (9q+), 9p-, +8, +21, +X, 6q-, abn(14q32) and +14. In contrast, only 4.4% of cALL were Ph+ aALL, with 42.3% of which being with additional abnormalities, including mainly abn(9p), abn(7p), -7, 17p- and +21. It is concluded that almost every chromosome is involved in the numerical and structural abnormalities and complex karyotypes are common. The significant difference of chromosome abnormality exists between aALL and cALL.
Adolescent
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Adult
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Aged
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Child
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Child, Preschool
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Chromosome Aberrations
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Cytogenetic Analysis
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Female
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Humans
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Infant
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Infant, Newborn
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Karyotyping
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Male
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Middle Aged
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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genetics
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Sample Size
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Young Adult
6.Significance of serum IgG4 and CA19-9 in diagnosis and antidiastole of IgG4 related cholangitis
Li-Ming TAN ; Xiao-Lin GUAN ; Si-Fan WU ; Wei ZHENG ; Hui-Ying FU ; Yi-Mei MENG ; Ting-Ting LONG ; Qiao-Hua WANG ; Yang WU ; Ting-Ting ZENG ; Yong-Jian TIAN ; Jian-Lin YU ; Juan-Juan CHEN ; Hua LI ; Li-Ping CAO ; Hui XU
Chinese Journal of Immunology 2018;34(3):431-435
Objective:To investigate the value of autoantibodies and serum levels of IgG4 and CA19-9 in the diagnosis of IgG4 associated cholangitis (IgG4-SC).Methods:Detect the serum IgG4 and CA19-9 of 41 clinical cases of IgG4-SC patients,162 clinical cases of non IgG4-SC patients and 40 healthy human serum samples by immunoassay and direct chemiluminescence methods, also detect the antinuclear antibodies (ANA),anti neutrophil antibody (ANCA),anti smooth muscle antibody (SMA) and anti mitochondrial antibody (AMA) of the above serum samples by indirect immunofluorescence and analyze the detection results.Results:①The positive rates of ANA,ANCA,SMA and AMA in patients with IgG4-SC were 41.46%,7.32%,0 and 2.44%.Among them,the positive rate of ANA was significantly different from that of the normal control group(P<0.01),and the positive rate of SMA and AMA was significantly different from that of non IgG4-SC group(P<0.01),and so as the positive rate of ANCA do with that of PSC group.②The number of serum IgG4 and CA19-9 increased samples were significantly compared with the normal control group (P<0.01);the area under the ROC curve (AUC) was 0.979 and 0.646,respectively,and P<0.05.Conclusion:The high level of serum IgG4 and CA19-9 and autoantibody detection are of great accuracy and important clinical value in the differential diagnosis of IgG4-SC.
7.Interaction between PSF and cytokeratin 18 mediates PSF relocation to cell membrane and maintains chemosensitivity of myeloid leukemia.
Si Mei REN ; Lu Yao LONG ; Cheng Shan XU
Journal of Peking University(Health Sciences) 2020;52(2):214-220
OBJECTIVE:
To identify the chaperone of polypyrimidine tractor-binding protein-associated splicing factor (PSF) in myeloid leukemia cells, and to explore the mechanism and redistributive pattern to cell surface of PSF in chemo-sensitive HL60 cells and resistant HL60/DOX cells.
METHODS:
The eukaryotic expression vector of PSF was transfected with liposomes transiently, then flow cytometry was used to detect the expression level of PSF on the cell surface 24 h, 48 h and 72 h after vector transfections. We constructed a chimeric expression vector, streptavidin binding peptide (SBP)-PSF, meanwhile this vector was transfected and made SBP-PSF fusion protein overexpress. In addition, we used streptavidin magnetic beads to precipitate the cellular chaperonin of PSF and then identified its chaperonin by mass spectrometry (MS). Lentiviral vectors containing cytokeratin18 (K18) interference sequences were transfected into 293T cells to prepare lentivirus. HL60 and HL60/DOX cells were infected with lentivirus to obtain stable interfering K18 cell lines. Next, flow cytometry was used to test the membrane relocation level of PSF. Together, these methods confirmed the similar or different mechanisms of the PSF redistributing to membrane synergistically mediated by K18 in HL60 and HL60/DOX cells.
RESULTS:
The expression of membrane relocated PSF was detected every day for three days (at the end of 24 h, 48 h and 72 h) after transient overexpression. The expressing rate of PSF on the cell surface was 22.4%±3.5%, 37.9%±6.0%, 58.3%±8.8%, respectively in sensitive HL60 cells, while that was 4.7%±0.5%, 3.9%±0.6%, 2.9%±0.6% , respectively in resistant HL60/DOX cells. The difference of expressing rate on each day was significant, P<0.01. We identified K18 detected by co-immunoprecipitation and mass spectrum assay which was the cellular chaperone of PSF. We found that K18 knockdown decreased the PSF expression level which redistributed on cell surface from 48.9%±5.4% to 6.2%±1.0% in sensitive HL60 cells, and from 9.11%±1.2% to 2.21%±0.51% in resistant HL60/DOX cells, respectively.
CONCLUSION
K18 is the intracellular chaperonin of PSF. The interaction of PSF and K18 mediates its redistribution to cell membrane in sensitive cells. While in resistant cells, PSF failed to relocate at the cell surface and accumulated in cells, which mediated resistance to chemotherapeutics.
Cell Membrane
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Doxorubicin
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Drug Resistance, Multiple
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Humans
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Keratin-18/metabolism*
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Leukemia, Myeloid
8.Analysis of Characteristics and Relevant Factors of 1 340 Cases of Intentional Injury Cases in Southwest China.
Wu LONG ; Chun Mei HU ; Si Si LI ; Song Lin XIE ; Jin Sai WU ; Lin Cong LI ; Chun Yue JIANG ; Bo JIN
Journal of Forensic Medicine 2019;35(4):433-436
Objective To investigate the characteristics and patterns of factors such as victims' information, injury tools and time of occurrence of intentional injury cases in southwest China. Methods One thousand three hundred and forty intentional injury cases from several places in southwest China from 2014 to 2016 assessed as minor injury level Ⅱ and above had been randomly selected. Data on victims' information, motives, injury tools, sites of occurrence, time of occurrence, injured parts and degrees of injury were classified and gathered, and then association analyses of motives and types of injury tools as well as degrees of injury and injury tools were made. Results Most of the victims were young adults between 20-50 years (65.2%), male (82.3%), rural household registration (62.8%); the motives were mainly dispute (45.8%). Injury tools were mostly blunt (54.6%) or sharp (36.0%). Specifically, injuries were mostly made bare-handed (36.9%) and by cutting tools (33.2%); the cases mainly occurred in public areas (59.0%). Cases occurred more frequently in January (11.3%), February (13.1%), March (11.6%) and from 22:00 to 01:00 every night. Injuries mainly involved the craniofacial region. The wounds were mainly assessed as minor injury level Ⅱ (61.6%). There was statistical significance in the difference of types of injury tools among cases with different motives (P<0.05). There was statistical significance in the difference of the distribution of injury tools among cases with different degrees of injury (P<0.05). Conclusion The occurrence of intentional injury cases in southwest China has potential patterns and relevant influencing factors. Prevention and analysis of such cases need to be comprehensively considered from the aspects such as victims' information, injury tools and time of occurrence.
Adult
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Age Distribution
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China
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Female
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Humans
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Male
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Middle Aged
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Motivation
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Retrospective Studies
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Sex Distribution
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Violence
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Wounds and Injuries/etiology*
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Young Adult
9. Applied anatomy of human periobital region
Loubin SI ; Mingzi ZHANG ; Xiao KONG ; Liu LIU ; Feng QIN ; Wenchao ZHANG ; Fei LONG ; Yang WANG
Chinese Journal of Plastic Surgery 2019;35(11):1114-1119
Objective:
To observe the anatomical layers and important vascular structures in the main periorbital regions of the human body, and to provide some anatomical basis for clinical periorbital fat injection.
Methods:
During January 2018 to December 2018, 12 (24 sides) cadavers (6 males and 6 females, 47.5±11.7 years old) were selected. Their orbital tissues were dissected routinely and microdissected. The important blood vessels and tissues around the orbit were measured by electronic vernier caliper. The related matters needing attention in fat injection filling were analyzed according to references.
Results:
The main structural areas around the orbit included eyelid, eyebrow and lacrimal groove deformities. The thickness of eyelid skin was (0.09±0.03) mm. The thickness of eyebrow skin was (3.45±0.38) mm. Vascular (diameter ranged from 1 mm to 3 mm) distribution was abundant in this area. The inner diameter of dorsal nasal artery, supraorbital artery and trochlear artery were (0.73±0.42) mm, (0.88±0.37) mm and (0.71±0.51) mm respectively. Facial artery, maxillary artery and superficial temporal artery with internal diameters of (2.96±0.88) mm, (1.92±0.33) mm and (1.35±0.15) mm, respectively.
Conclusions
The entrance of upper eyelid injection is usually in the eyebrow tail or middle eyebrow, and fat is injected into the deep surface of orbicularis oculi muscle. The injection range is limited to the medial 2/3 of upper eyelid, the medial 1/3 of lower eyebrow and the lateral part of eyebrow. It is suggested that single layer microinjection of fat (0.5 ml to 1.5 ml) could be used. Lower eyelid fat transplantation is mainly used to correct deformities at the eyelid-cheek junction. The aim is to reduce the V-shaped deformity at the eyelid-cheek junction by increasing the fullness. Injection can be made by blunt needle into the inner, outer and middle part of the deformity. Fat can be injected into SOOF layer or periosteum in the palpebral and cheek sulcus area. The injection volume is 0.5-1.0 ml.
10.Laboratory confirmation of the first influenza A (H1N1) imported case in Mainland China.
Wei WANG ; Ming PAN ; Guo-Hui CHANG ; Xiao-Dan LI ; Tian-Shu LI ; Cheng-Feng QIN ; Na JIA ; Le-Ying WEN ; Rong-Bao GAO ; Wen-Bin TONG ; Shu-Sen HE ; Da-Yan WANG ; Jun-Feng GUO ; Yu LAN ; Lei YANG ; Xiang ZHAO ; Xi-Yan LI ; Zi LI ; Shu-Mei ZOU ; Qing-Yu ZHU ; Yuan-Ji GUO ; Wu-Chun CAO ; De-Xin LI ; Yue-Long SHU
Chinese Journal of Virology 2009;25 Suppl():4-7
The clinical throat swab specimen of an imported suspected case of influenza A (H1N1) was detec ted with real-time PCR, RT-PCR and subsequently confirmed by gene sequencing. The presence of influ enza A (H1N1) virus confirmed the first case with A (H1N1) infection in Mainland China.
China
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Humans
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Influenza A Virus, H1N1 Subtype
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classification
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genetics
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isolation & purification
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Influenza, Human
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virology
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Molecular Sequence Data
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Phylogeny