Objective To establish a HPLC method for determining of the content of palmatine hydrochloride in Fuke Qianjin Tablets. Methods HPLC was performed on Shimadzu-C18 column. The mobile phase consisted of 0.05 mol·L-1 sodium acid phosphate (pH=3, adjusted by phosphoric acid)-acetonitrile (72 : 28). The detection wavelength was at 265 nm. Results The linear correlation was in the range of 0. 051 μg～0. 255 μg(r=0. 999 9). The average recovery was 100. 04 %, and RSD=1.03 % (n=5). Conclusion The method is accurate and specific.

Objective To establish a HPLC method for determining of the content of palmatine hydrochloride in Fuke Qianjin Tablets. Methods HPLC was performed on Shimadzu-C18 column. The mobile phase consisted of 0.05 mol? L-1 sodium acid phosphate (pH=3, adjusted by phosphoric acid)-acetonitrile (72 ∶ 28). The detection wavelength was at 265 nm. Results The linear correlation was in the range of 0.051 ? g～ 0.255 ? g(r=0.999 9). The average recovery was 100.04 % , and RSD=1.03 % (n=5). Conclusion The method is accurate and specific.

For pharmaceutical industries, clinical data is one of the most valuable deliverables. It is also the basis of analysis, submission, approval, labeling and marketing of a drug product. To ensure the integrity and reliability of clinical data, a scientific standardized quality control (QC) has to be established at each step of a clinical trial. Data validation is conducted to ensure the reasonability and compliance of clinical data by checking data quality before the data is statistically analyzed. This paper focuses on purpose of data validation, creation of data validation plan, rationale of data validation, types of data validation and performance of user acceptance testing on clinical database.
Clinical Trials as Topic ; standards ; Data Accuracy ; Data Collection ; standards ; Databases, Factual ; Quality Control ; Reproducibility of Results

To determine the content of astragaloside IV in Xiao' er Weijining Mixt ure, TCL conditions were optimized by comparing various refined methods. And the y were as follows: the underlayer of CHCl3-CH3OH-H2O(65∶35∶10) below 10 ℃was developed at 15～18℃ and sprayed with 10%H2SO4-C2H5OH,the heating tempe rature was 105℃ for 3～5 min,and λS=400 nm, λR =700 nm.

Objective To study whether mechanical noise acting on the sacculus could enhance the SNR of the 8th nerve’s response.Methods Driving PZT by adding white noise of various level to periodic signals(f=100 Hz),the directly mechanical stimuli was give to the five sacculus submerged in a solution containing perilymph-like, and the afferent activity the 8th nerve was recorded.Results The SNR of the nerve signal was improved by addition nanometer-level noise to the periodic stimuli in all responsive animal(4.1 dB, on average).It was found that 2.3 nm of mechanical noise enhanced the response of the saccular nerve.Conclusion The addition of a few nanometers of noise to a periodic stimuli leads to a substantial improvement in the SNR of the nerve’s response.

Platelet membrane glycoproteins play a key role in the processes of platelet adhesion,activation and aggregation and thrombosis.Many studies have shown that platelet membrane glycoprotein gene polymorphisms are associated with ischemic stroke.This article reviews the relationship between platelet membrane glycoprotein gene polymorphisms and ischemic stroke.

[Objective] To investigate the clinical effects in the treatment of gluteus muscles contracture of small incision insidious lysis and three steps rehabilitative drills.[Method]One hundred and eighteen patients were treated with small incision insidious lysis and three steps rehabilitative drills(treatment group),and fifty-six patents with gluteus maximus lysis(control group)from February 1988 to February 2006.Clinical classification of gluteal muscle contracture,mechanism of crook-lateral and spread-lateral limb cross-leg tests designed by the author,main points of small incision insidious lysis and implement of three steps rehabilitative drills were evaluated.[Result]The cases were followed up for 1~8 years,clinical outcomes were evaluated by the Huang Yao-tian's scoring system.The early clinical effect were evaluated after 45 days of operation.All the results showed statistically significant difference(

Objective To investigate the relationship between the posterior vitreous detachment (PVD) and the diabetic macular edema (DME). Methods A total of 169 eyes with DME which were diagnosed by funduscopy,fundus fluorescein angiography (FFA) and optical coherence tomography (OCT) were retrospectively analysed. PVD and partial PVD were detected by OCT and biomicroscopy. The characterizations of OCT images of DME were analysed. Results In 169 eyes, 156 (92.3%) had no PVD with the average thickness of fovea of 297 ?m; 11 (6.5%) had complete PVD with the average thickness of fovea of 229 ?m; 2 (1.2%) had partial PVD with the average thickness of fovea of 347 ?m. Conclusions Only a few PVD were found in DME eyes, so traction of PVD may not be the main cause of DME.

Objective To investigate the course of the idiopathic macular hole (IMH) clinically diagnosed as at stage Ⅰ-Ⅳ by optical coherence tomography (OCT), and analyze the relationship between the posterior vitreous detachment (PVD) and the course of IMH. Methods Hadn′t undergone any operation, periodical OCT were performed on 72 eyes of 58 patients with IMH at stage Ⅰ-Ⅳ confirmed by Gass standard and the features of OCT images. Results The longest follow-up period was 43 months (average 13.4 months), and the examine times of OCT on each eye were between twice to 10 times (average 4.7 times). During the follow-up period, 23 eyes were in stage I in which 9 (39.1%) developed to stage II and 2 had recovered normal curve of fovea after PVD; 19 were in stage II in which 13 (68.4%) developed to Ⅲ-Ⅳ and 1 had closed hole after PVD; 11 were in stage III in which 5 (45.5%) developed to stage IV and 1 had partly closed hole 12 months later. The images of OCT showed that the process of macular hole was consistent with the course that vitreous depart from retina from the circumference of fovea till entire posterior detachment.Fifteen affected eyes in this series of patients had undcrygong surgical treatment due to serious progression of IMH in follow-up period. Conclusions There is a close relationship between the formation and development of macular hole and the occurrence of PVD. OCT can show the progress of the macular hole directly and offer an important technique in diagnosis, classification and surgical treatment of IMH.

To determine the content of astragaloside IV in Xiao'er Weijining Mixture, TCL conditions were optimized by comparing various refined methods. And they were as follows: the underlayer of CHCl 3-CH 3OH-H 2O(65∶35∶10) below 10℃was developed at 15～18℃ and sprayed with 10%H 2SO 4-C 2H 5OH,the heating temperature was 105℃ for 3～5 min,and ?S=400 nm, ?R =700 nm.