1.Analysis of the variation of 32 HIV-1 V3 loop from infected Chinese blood/plasma donors.
Lli DAI ; Jun-feng LU ; Xiao-jie HUANG ; Ying SHI ; De-xi CHEN ; Hao WU
Chinese Journal of Experimental and Clinical Virology 2008;22(1):27-29
OBJECTIVETo characterize the variation in V3 loop of HIV-1 B'strains circulating in Chinese blood donors.
METHODSThe c2-c3 regions of the HIV envelop gene were amplified by nest-PCR from 32 HIV-1-infected blood donors in He Nan province in China. The BIOEDIT and MEGA software are used to analyze the sequences of V3 loop.
RESULTSThere are five types of central motifs of the 32 samples, in which GPGR and GPGQ are most common. More variations associated with T tropic/SI phenotype can be seen in AIDS group.
CONCLUSIONThe V3 tip motifs of HIV-1B' strains circulating in Chinese blood donors are various, the different characterization of V3 loop between AIDS and asymptomatic patients indicates different biological phenotype and pathogenesis which warrant additional investigation.
Adult ; Aged ; Amino Acid Motifs ; Amino Acid Sequence ; Blood Donors ; China ; Female ; Genetic Variation ; genetics ; HIV Envelope Protein gp120 ; analysis ; chemistry ; genetics ; HIV Infections ; blood ; virology ; HIV-1 ; chemistry ; classification ; genetics ; isolation & purification ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; Oligopeptides ; chemistry ; Phenotype
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