Objective To observe the effect of severe hypertensive cerebral hemorrhage with minimally invasive technology. Method Fifty patients of severe hypertensive cerebral hemorrhage treated by minimally invasive puncture combined early systemic rehabilitation (treatment group), and 49 patients with conventional treatment as control group. Results (1)The survival number were 37 cases in treatment group and 28 cases in control group. The survival patients with GCS after 4 weeks in treatment group was significantly higher than that in control group (P < 0.01). (2) FIM score of treatment group and control group on admission [(58±12) scores vs (60±11) scores] and the 14 th day [(64±13) scores vs (62±12) scores] after treatment, there was no significant difference (P > 0.05), but the FIM score of treatment group at the first month [(76±16) scores vs (65±14) scores] and the third month [(90±22) scores vs (69±18) scores] after treatment were significantly superior to eontrol group (P < 0.05). (3) NIHSS score of treatment group and control group on admission were (17.5±3.5) scores and (16.9±3.4) scores (P > 0.05), the NIHSS score of treatment group at the 14th day,the first month and the third month were significantly superior to control group [(14.3±3.3) scores vs (16.2±3.5) scores, (12.1±2.4) scores vs (15.7±3.4) scores, (9.0±3.4) scores vs(12.2±23.6) scores, respectively] (P< 0.05). Conclusion The treatment of severe hypertensive cerebral hemorrhage with minimally invasive percutaneous is safe and effective, the effect is superior to conventional medical treatment.

Objective To investigate the functional status of adrenal cortex status in newborn with septic shock, and explore the diagnostic criteria of relative adrenal insufficiency (RAI) and catecholamine resistance in shock. Methods Fifty newborns with septic shock, 28 males and 22 females, admitted into neonatal intensive care unit (NICU) of Hangzhou Second People's Hospital from Jan. 2005 to Jun. 2009, were involved in the present study. Serum cortisol levels of the newborn patients were measured with radio immunoassay at baseline and 30 and 60 minutes after-administration of a low-dose (1?g) of adrenocorticotrophic hormone (ACTH). The patients were divided into two groups by maximal cortisol increment levels: normal adrenal function (NAF) group and RAI group. Some clinical factors, for example, the fluid demands on 1st day, shock duration, mortality, neonatal critical illness score (NCIS) and the incidence of catecholamine resistance shock, were observed. According to prognosis these newborn patients were then divided into two groups: nonsurvivors and survivors. The serum cortisol levels were compared after ACTH test between the two groups. Results Nineteen RAI and 31 NAF were observed in the 50 cases. The fluid demands on 1st day, shock duration and mortality were higher, while NCIS was lower in RAI group than in NAF group (P

Objective: To review the experience of one-stage surgical management of thoracic aortic aneurysm associated with coronary artery disease. Methods: From June 1999 to July 2002, fifteen patients (all the male, average age 57.21 year-old, ranged 40-66 year-old) underwent thoracic aortic aneurysm repair and concomitant coronary revascularization. The diagnosis are aortic root aneurysm in 5 cases,ascending aortic aneurysm in 2,arch aortic aneurysm in 3,and aortic dissecting in 5. 14 patients received operation through midsternotomy,including ascending aorta replacement in 2, Bentall technique in 4, David technique in 1 and total arch replacements in 3 patients. Left thoracotomy and off-pump CABG was done in 1 case. Results: 1 patient died in 30 days after operation. Followed up for a mean duration of 17 months,there were no late death and no reoperation and no related events occurred. Conclusion: Thoracic aortic replacement and CABG (coronary artery bypass grafting) could be done at one-stage. Concomitant coronary revascularization may aggressively obviate CAD events.

Objective To investigate the health security requirements on food and drinking water during military training on the plateau,and explore ways to improve military health security support capability.Methods Seven army units were randomly chosen.By means of on-site check and sample detection,both internal and external environmental factor changes and health and safety conditions of food and drinking water were surveyed on the plateau.Internal and external standard methods were used to test the stability of portable instruments.Results There was considerable difference in diurnal temperature and relative humidity between indoors and outdoors.In two field units,raw materials of foods were purchased in a centralized manner and suppliers were fixed,they were purchased independently in the othev five units. Facilities of food storage and transportation were lacking in all the seven units.In one of the fifty-three food samples, residues of pesticides exceeded the national standard.Among the three water samples,the level of turbidity,ammonia nitrogen and nitrate nitrogen was lower than the standard limits,but free residual chlorine and total chlorine were not detected.The plateau environment had no significant effect on spectrophotometers,but had significant influence on enzyme-linked immune detectors.Conclusion There are potential health hazards to training troops on the plateau that arise from foods and drinking water.Improvement of management and equipment adaptability is needed to further enhance the field health security efficacy.

Objective To evaluate the value of MRI diffusion tensor imaging (DTI) on diagnosis of traumatic brain injury(TBI). Methods Twenty two patients with TBI 1 to 7 days post-injury and 14 healthy controls were studied with DTI and conventional MRI. The fractional anisotropy (FA) was quantified from different regions of interest (ROI) including the genu of corpus callosum (CC), the splenium of CC, the genu of internal capsule (IC) and the posterior limb of IC bilaterally. The FA value of the same ROI was compared between TBI group and control group, and FA value of the lesion side was compared with the mirror healthy side in TBI group. Correlations between the FA and Glasgow coma scale (GCS) in TBI patients were analyzed with Pearson linear correlation. Results Compared with control group, the FA value decreased significantly in each ROI (P<0.01). Compared with the healthy side, FA value was also significantly lower in lesion side in TBI group (P<0.01). The FA value in all the sites were not correlated with GCS in TBI group (P>0.01).Conclusion DTI is sensitive for detecting the acute traumatic injury of white matter and evaluate the degree of injury. It offers the possibility to diagnose TBI earlier and accurately.

Objective To establish the normal reference ranges of clinical pathology for Beagle dogs in the Good Laboratory Practice ( GLP ) system.Method Sixteen biochemical indexes , seventeen hematological indexs and three coagulation function indexes of 117 Beagle dogs were detected , and the mean value of each index and the normal reference ranges were calculated and compared .Results Only alkaline phosphatase ( ALP ) from the biochemical items was significantly different between males and females (P<0.01),which was higher among males than among females .Three in-dexes of hematology were significantly different between males and females (P<0.01),with red blood cell(RBC), hemo-globin(HGB)and hematocrit(HCT)lower among males than among females.The coagulation function items were not signif-icantly different between the two sexes .Conclusion Some indexes of clinical pathology were significantly different between males and females , which should be considered during statistic analysis on toxicity .Our study has established the normal reference range of clinical pathology for Beagle dogs in the GLP system , which provides reference for toxicity tests .

BACKGROUND:There are various methods for the treatment of osteonecrosis of femoral head, but there is no satisfactory method to promote the repair of osteonecrosis of femoral head. In recent years, bone marrow
mesenchymal stem cel transplantation for the treatment of osteonecrosis of femoral head has achieved certain effect.
OBJECTIVE:To review the application progress and problems of bone marrow mesenchymal stem cel transplantation for the treatment of osteonecrosis of femoral head.
METHODS:A computer-based online search was performed in PubMed database, Wanfang database and CNKI database for the related articles from 1999 to 2012. The articles on the isolation, culture, differentiation, labeling and in vivo tracing of bone marrow mesenchymal stem cel s were selected, as wel as the basic and clinical
researches on bone marrow mesenchymal stem cel transplantation for the treatment of osteonecrosis of femoral head. A total of 39 articles were included for review.
RESUTLS AND CONCLUSION:At present, the method for the isolation of bone marrow mesenchymal stem cel s includes adherence screening method, density gradient centrifugation, flow cytometry separation and magnetic activated cel sorting method;the commonly used method for cel labeling and tracing includes isotope tracing method, antigen labeling method, antigen labeling, fluorescent labeling and MRI contrast enhancer labeling
method. The method for the treatment of osteonecrosis of femoral head with bone marrow mesenchymal stem cel s includes pith dril ing decompression combined with bone marrow mesenchymal stem cel injection and
transplantation, intervention plus bone marrow mesenchymal stem cel transplantation, gene transfection combined with bone marrow mesenchymal stem cel transplantation and tissue engineering technology of bone marrow mesenchymal stem cel s. Although, the research on the bone marrow mesenchymal stem cel transplantation for the treatment of
osteonecrosis of femoral head has achieved great progress, there are stil problems needed to be further solved.

Objective Methicillin-resistant Staphylococcus aureus (MRSA) is a growing public health concern that has been associated with pediatric fatalities. This study investigated the genotypes of staphylococcal cassette chromosomal mec (SCCmec) and Panton-Valentine Leukocidin (PVL) in MRSA strains isolated from Shanghai Children's Hospital by PCR. Methods A total of 30 strains of MRSA were isolated from various clinical specimens from October 2005 to June 2006. The antimicrobial susceptibility was measured by agar diffusion method. SCCmec typing was conducted using a novel multiplex PCR assay allowing for concomitant detection of methicillin resistance (mecA gene) to facilitate detection and classification of all currently described SCCmec typesⅠ, Ⅱ, Ⅲ, Ⅳa, b, c, d andⅤ. PVL gene was also determined by PCR. Results mecA gene was positive in all the strains. SCCmecⅡ was identified in 6(20.0%) isolates, SCCmecⅢ in 15(50.0%) isolates, SCCmecⅤ in 2 and SCCmecⅣa in 1 isolate. Six MRSA strains were non-typeable. The isolates with SCCmecⅡ or SCCmecⅢ were resistant to multiple antibiotics. The strains harboring SCCmecⅣa or SCCmecⅤwere susceptible to all antibiotics except β-lactams. Eleven (36.7%) isolates were PVL positive. The genotypes and subgenotypes of staphylococcal chromosomal cassette mec of eleven PVL-positive MRSA were SCCmecⅡ(1 isolates), SCCmecⅢ (5 isolates), SCCmecⅣa (1 isolate), SCCmecⅤ (2 iso-Lates) non-typeable (2 isolates). Conclusions SCCmecⅡ and SCCmecⅢ are the major genotypes of MRSA in our hospital. These isolates are multi-resistant to antibiotics. The prevalence of PVL gene is higher in SCCmecⅡ- or SCCmeⅢ-positive MRSA. The isolates with SCCmecⅡ or SCCmecⅢ were resistant to multiple antibiotics.

Objective To investigate the effect of perhexiline injection on oxygen free radical in old patients with heart failure(CHF). Methods 32 healthy parsons were taken as controls. 58 old patients with CHF were ran-domly divided into two groups. The treatment group received both basic therapy and perhexiline injection transfusion for 14 days. Malondialdehyde (MDA) ,total antioxygen capacity(TAOC) ,oxidation-low-density-lipoprotein-cholester-ol (OX-LDL-C) and superoxide dismutase(SOD) were measured before the therapy and after the therapy. Results MDA [ (5.59±1.95 )μmol/L and OX-LDL-C [ ( 808.64 ± 156.34) μg/L] were significantly higher in the old patients with CHF] than control group [ (4.04 ± 0.67 μmol/L ) and ( 538.67 ± 125.43 ) μ/L ] ( P < 0.05 ) ; SOD [ (68.69 ± 20.43 )μU/L ] and TAOC [ ( 7.72±2.13 ) kU/L ] were significantly lower in the old patients with heart failure than the matched group [(105.67±13.86 )μU/L and (9.68±1.86 ) kU/L ] ( P <0.05 ), which were posi-tively associated with CHF severity. After the improvement of the cardiac function, MDA, OX-LDL-C;SOD and TAOC recovered significantly. Conclusion The therapeutic action of perhexiline injection in old patients with CHF can re-ly on the promotion of eliminating oxygen free radical inside organism.