Adenocarcinoma ; genetics ; metabolism ; pathology ; Biopsy ; CD56 Antigen ; metabolism ; DNA-Binding Proteins ; metabolism ; Gene Deletion ; Humans ; Keratin-7 ; metabolism ; Lung Neoplasms ; genetics ; metabolism ; pathology ; Male ; Middle Aged ; Receptor, Epidermal Growth Factor ; genetics ; metabolism ; Small Cell Lung Carcinoma ; genetics ; metabolism ; pathology ; Synaptophysin ; metabolism ; Transcription Factors

OBJECTIVETo develop a high performance liquid chromatography coupled with a photodiode array detector (HPLC-DAD) method for simultaneous determination of 7 nucleosides and nucleobases in Fritillaria taipaiensis.

METHODThe analyses were performed on an Agilent Zorbax-SB-Aq-C18 column (4.6 mm x 250 mm, 5 microm) eluted with water and methanol in gradient mode. The flow rate was 1.0 mL x min(-1). The detection wavelength was 260 nm. The temperature of sample manager was set at 25 degrees C, and the injection volume was 20 microL.

RESULTThe investigated compounds including uracil, cytidine, uridine, guanosine, thymidine, adenosine and adenine were shown good linearity (r > or = 0.999 8) over the tested ranges. The average recoveries were within 96.96% - 103.5% with RSD < or = 3.8%.

CONCLUSIONThe accuracy, stability, repeatability and average recovery of the method are satisfying, and the seven nucleosides and nucleobases components in F. taipaiensis can be rapidly and accurately quantified by HPLC-DAD. This work provided helpful information for comprehensive quality evaluation of F. taipaiensis.

Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; Fritillaria ; chemistry ; Nucleosides ; analysis ; Reproducibility of Results ; Water ; chemistry

Obj ective To explore the significance of serum lipoprotein and high sensitivity C-reactive protein (hs-CRP)level for the patients with type 2 diabetic nephropathy.Methods 174 patients with type 2 diabetic mellitus were divided into diabetic mellitus group,early diabetic nephropathy group and clinical diabetic nephropathy group,according to the urinary albumin excre-tion rate (UAER).60 healthy individuals were chosen as control group.Lipoprotein (α)[Lp(α)], high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C)and hs-CRP were detected,and the results were analyzed statistically.Results The level of Lp (α), hs-CRP were significantly higher in patients with diabetic mellitus than those in control group (P<0.05).Among the three groups with diabetic mellitus,the level of Lp(α)and hs-CRP got progres-sively significant higher with the increase of UAER (P<0 .05 )whereas the level of HDL-C and LDL-C did not find significant difference(P>0 .05 ).There were linear positive correlation between Lp(α),hs-CRP and UAER levels(r= 0.8069,0.8989).Conclusion Lp(α),hs-CRP levels may be related to the progression of diabetic nephropathy,and their detection had important clinical sig-nificance in early diagnosis and prognostic evaluation of type 2 diabetic nephropathy.

Obj ective To explore the significance of serum lipoprotein and high sensitivity C-reactive protein (hs-CRP)level for the patients with type 2 diabetic nephropathy.Methods 174 patients with type 2 diabetic mellitus were divided into diabetic mellitus group,early diabetic nephropathy group and clinical diabetic nephropathy group,according to the urinary albumin excre-tion rate (UAER).60 healthy individuals were chosen as control group.Lipoprotein (α)[Lp(α)], high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C)and hs-CRP were detected,and the results were analyzed statistically.Results The level of Lp (α), hs-CRP were significantly higher in patients with diabetic mellitus than those in control group (P<0.05).Among the three groups with diabetic mellitus,the level of Lp(α)and hs-CRP got progres-sively significant higher with the increase of UAER (P<0 .05 )whereas the level of HDL-C and LDL-C did not find significant difference(P>0 .05 ).There were linear positive correlation between Lp(α),hs-CRP and UAER levels(r= 0.8069,0.8989).Conclusion Lp(α),hs-CRP levels may be related to the progression of diabetic nephropathy,and their detection had important clinical sig-nificance in early diagnosis and prognostic evaluation of type 2 diabetic nephropathy.