There are a variety of inherited metabolic diseases (IMD), the incidence is low and clinical manifestations are not char-acteristic. IMD primarily affect not only the nervous system, but also the blood system, which characterized by the abnormalities of blood cells and bone marrow. Because of some clinician's lack of this knowledge, some patients are prone to be misdiagnosed. To pro-mote early diagnosis, this article reviews five groups of IMD (lysosomal storage disease, vitaminopathies, organic aciduria, amino-acidopathies and others) and different hematological abnormal manifestations.

Objective To discuss the influence of individualized health education based on combination of TCM and western medicine on the life quality of patients with diabetes mellitus in order to supply novel idea and method for carried out education about diabetes mellitus. Methods Hospitalized patients (140 cases) were randomized into the control group and the test group with 70 cases in each group. Both groups received routine diabetes drugs treatment. The patients and their family members received individualized health education based on combination of TCM and western medicine during their hospitalization days and 6 months after discharge. SF-36 inventory and diabetes cognitive questionnaire was adopted to investigate the two groups. Results The diabetes cognitive degree of the test group was higher than that of the control group (P<0.01). Six items such as social function, systemic health, body pain, psychological health, vigor and emotional role function of the test group was different from those of the control group (P<0.05). Conclusion Individualized health education based on combination of TCM and western medicine contributed to the improvement of patients' life quality.

Objective To study the characteristics of complement expression and the effects of atorvastatin on the complement in rats after the cerebral ischemic infarction.Method One hundred and sixty adult healthy Sprague-Daweley rats were randomly divided into normal group,sham-operated group,ischemia group and treat group.The ischemia group and treatment group were sub-devided into 6 h,12 h,24 h,48 h,3 d,5 d,2week,respectively.The middle cerebral artery occlusion(MACO)model was induced by using filament method,and the thread Was withdraw from the middle cerebral artery after occlusion 2 h.Rats in the sham-operated groupdid not have thread inserted.After wakefulness,the animals The successful of animal model produced was evidenced by the paralysis of contralateral limbs.The animals model in the treatment group treated with Atorvastatins 10 mg once a day for 2 weeks and,rats in the ischemia group did not receive Atorvastatin.The neurological deficit scoring was measured at different intervals in the ischemia and treat ment groups.The expressions of complement C1q and C3 d protein in the brain of the rats in 4 groups were measured by immunohistochemical methods.Result There were a few complement expression in the brain of normal rats,showing no significant difference observed between sham-operated group and normal group(P＜0.05).The complements(C1 q and C3 d)expressied increasubgkt after cerebral ischemia injury,peaked 24 h after ischemia,and returned to normal levels 5 d after ischemia.The expression of complement C1 q and C3 d in the ischemia group were significanfly higher than those in sham-operated group(P＜0.05),The expression of complement C1 q and C3 d in treat ment group were signnificantly lower than those in the ischemia group(P＜0.05).The neurological deficit score in treatment group were signnificantly lower than that of ischemia group.Conclusions The expressions of complement C1 q and C3 d were increase gradually after cerebral ischemia in the rats,Atorvastatin Can inhibit the complement activation,improve neuro logical function of rats.

Objective To study the effects of erythropoietin(EPO) and angiotensin receptor blocker on the expression of eNOS and Bcl-2 after cerebral ischemia.Methods The model of focal cerebral ischemia was made by occluding middle cerebral artery(MCA) for 2h and reperfusing for 48h in healthy Sprague-Dawley rats.The rats received EPO 3 000u · kg-1 · d-1 or valsartan 40mg · kg-1 · d-1 by intraperitoneal injection just before the beginning of reperfusion and after reperfusion.The expression of eNOS and Bcl-2 was detected.Results Compared with ischemia group,Bcl-2 and eNOS expressions in EPO-treated group and EPO-ARB treated group were significantly increased (P ＜ 0.05).eNOS expression in ARB-treated group was significantly increased (P ＜ 0.05),Bcl-2 expression was up-regulated,but it had no significant difference.Conclusion EPO had protective effects on cerebral ischemia-reperfu-sion injury in rats,which partially mediated by up-regulating of Bcl-2 and eNOS expression.Angiotensin receptor blocker had protective effects on cerebral ischemia-reperfusion injury in rats,which partially mediated by up-regulating of eNOS expression.

Observation revealed the abnormalities (increased CD 4 and Th, decreased Tc) in patients of Graves′ disease, and these abnormalities were not significantly adjusted after 6 weeks of treatment with methimazole (MMI), or with MMI and methotrexate (MTX), though the soluble interleukin-2 receptor and thyroid autoantibodies slightly decreased in both groups. However, the addition of MTX did not show any better effects than MMI alone in the treatment.

Objective To investigate the risk factors for postoperative cognitive dysfunction (POCD) and nursing intervention in patients undergoing laparoscopic radical hysterectomy. Methods One hundred patients undergoing laparoscopic radical hysterectomy were included, surgical history, general anesthetics way, education, family factors(alone), body mass index, age, duration of anesthesia, dose of sufentanyl, hematocrit, the time for regaining orientation, dysphoria, postoperative pain degree were recorded. The occurrence of POCD was observed at 7 days (early period) and 3 months (later period) after surgery. Logistic regression analysis was used to examine the risk factors for POCD at early period and later period. Results The incidence of POCD at early period was 29.0%(29/100). Logistic regression analysis of surgical history, general anesthetics way, body mass index, age, hematocrit, the time for regaining orientation, postoperative pain degree in patients, general anesthetics way (OR=2.821, 95%CI 1.099-7.240) and postoperative pain degree(OR=2.292, 95%CI 1.129-4.654) were the significant risk factors for POCD at early period. The incidence of POCD at later period was 7.0%(7/100). Logistic regression analysis of education and family factors(alone) in patients, family factors(alone) was the significant risk factor for POCD at later period (OR=5.517, 95%CI 1.010-30.126). Conclusions General anesthetics way and postoperative pain degree are the significant predictors for POCD in patients undergoing laparoscopic radical hysterectomy during hospitalization, and family factors(alone) is the risk factor for POCD at 3 months after surgery, and active nursing intervention could reduce the occurrence of POCD.

Objective To overcome the fact that SRV-NM virus can only multiple and amplify through partially pu-rified jaagsiekte retrovirus inoculated intratracheally in sheep but it cannot be augmented using in vitro cell culture, we con-structed JSRV-NM pseudovirions based on high efficiency packing system of lentivirus. Methods Lentivirus of three high efficiency packing plasmids system pMD.G, pCMV-HIV 8.2 and pHIV-eGFP was developed, and JSRV-NM-env coated plasmid pCMVJSRV-NM was used to substitute VSV-G virus coated plasmid pMD.G then co-transfected into 293T cells to replicate, package and produce restructured JSRV-NM pseudovirions. Gene expression of pseudovirion was determined through WPRE using real time PCR; Virus infectivity was detected through inoculating JSRV-NM pseudovirions into 24 pore plates. Results We construct JSRV-NM pseudovirions successfully based on the lentivirus system. JSRV-NM pseudo-virions can also be concentrated to higher titer (108 TU/mL detected by real time PCR by ultracentrifugation without signifi-cant loss of activity. JSRV-NM and VSV-G pseudovirions infected on Hela cells (both MOI= 3) respectively and no obvi-ous difference were shown on their infection efficiency detected by real time PCR. Conclusion Based on lentivirus system, JSRV-NM pseudovirions can be multipled and amplified in 293T cell culture in vitro. JSRV-NM pseudovirions is stable without loss its infection activity and the requirements of biological laboratory safety II was also met. JSRV-NM pseudoviri-ons will provide a useful tool for further study of JSRV-NM-env infection across species or its induction of lung adenocarci-noma.

Objective To investigate the prevalence of obesity and metabolic syndrome(MS)of children and adolescents in Longquan mountainous area in Zhejiang province.Methods A representative sample involving 2 135 children and adolescence aged 10 to 15 years were randomly surveyed and a total of 2 125 had available data(male/female ratios as:1 109/1 016).Using the standard methods,we measured the weight,height,waist circumference,hip circumference,blood pressure,detected fasting plasma glucose (FPG),high density lipoprotein cholesterol (HDL -C),triglyceride(TG),total cholesterol(TC),and calculated non-high density lipoprotein cholesterol(non-HDL). The prevalence of obesity and MS among the 10 to 15 years old children and adolescence in Longquan was compared with that in six cities in China(Beijing,Tianjin,Shanghai,Zhejiang,Chongqing and Guangxi).Results The preva-lence of obesity was 4.7% in 10 -15 -year -old teenage,in which the male obesity prevalence was 6.3%(70/1 109),female obesity prevalence was 3.0%(30 /1 016).The prevalence of overweight was 9.4% and the prevalence of boys was 11.0%,while the girl was 7.6%.The prevalence of obesity and overweight was 16.4%(299/2 125).The prevalence of MS was 2%(42/2 125)in the survey,but 42.0%in the obesity.The prevalence of obesity in Longquan(4.7%)was lower than that in the national six cities(8.1%)among the children and adoles-cents from 10 to 15 years old(χ2 =31.09,P=0.000).But in the obesity students,the prevalence of MS(42.0%)in Longquan was higher than that in six cities(28.8%)(χ2 =5.43,P=0.02).Conclusion The prevalence of obesity in Longquan mountainous area was lower than that in the national six cities among the children and adolescents from 10 to 15 years old.However,the prevalence of MS among the obesity in Longquan was higher than that in six cities.

Objective To observe the antitumor activity of sedum lineare thunb(SLT) and it′s affection on antioxidation and tumor immunology in S180‐bearing mice .Methods S180‐bearing mice model were established by subcutaneously inoculating S180 ascitic tumor into the right armpit .From the second day ,the mice were treated with normal saline ,cyclophosphamide (50 g/kg) or two doses of SLT (4 ,8 g/kg) through intragastric administration ,and the process extent 14 d .After the last administration ,the mice body weight and the index of tumor ,spleen and thymus were calculated ,classification of white blood cells in peripheral blood was tested ,and the activity of SOD and GSH‐Px as well as the content of MDA and NO in serum were measured .Results The body weight of mice treatmented with SLT was dramaticlly higher than those of the CTX (P< 0 .05) .Compare with the model group ,SLT groups showed decreases in tumors index (P<0 .05) ,spleen index (P<0 .05) ,percentage of peripheral blood neutro‐phil (P<0 .05) and monocyte (P<0 .05) ,and increases in thymus index (P<0 .05) and percentage of lymphocyte (P< 0 .05) . The activity of SOD and GSH‐Px and NO level in the SLT groups mice′s serum were significantly higher than those of the model group (P<0 .05) ,while the content of MDA in the SLT groups mice′s serum was markedly lower than those of the model and CTX groups(P<0 .05) .Conclusion SLT has antitumor activity in S180‐bearing mice bysuppressing oxidative stress and modula‐ting tumor immune .

Objective: To evaluate the effects of 650 nm-10.6 μm combined laser in patients with knee Osteoarthritis (OA) and to determine whether the combined laser provides greater pain relief and improved function compared with red light. Methods: Forty-eight patients with knee OA were randomly allocated to two groups (24 per group), receiving 20 rain irradiation with 650 nm -10.6 μm combined laser or red light emitting diode respectively on point Dubi (ST 35) 3 times a week for the first course (2 weeks) and twice a week for the second one (4 weeks). The main outcome measures were WOMAC (Western Ontario and McMaster Universities Osteoarthritis Index) scores. In addition, patients' global assessment, adverse effects and validation of patient blinding were analyzed. Results: All the patients completed the first course, but 12 were lost during the second one. Due to the high dropout rate by the second course, only the data acquired from the first course could be analyzed. No differences of general data of patients and WOMAC scores were found in between-group comparison before treatment (P>0.05). The WOMAC scores of patients in both combined laser group and red light group reduced significantly compared to baseline by the end of the first course (P<0.01). There were no significant differences on the reduction rate of WOMAC scores between two groups (P>0.05). Neither the patients' global assessment nor the dropout rate showed statistical differences between two groups (P>0.05). There was no difference between two groups in patients correctly guessing the treatment assignment (P>0.05). There was no significant difference in the reduction rate of WOMAC scores and the patients' global assessment between patients who guessed their assignment (P>0.05). Conclusion: Both combined laser and red light irradiation are beneficial to patients with knee OA. But as the statistical indifferences between two groups, the authors can't conclude from this study whether the combined laser is more effective.