This article makes a discussion on how to use the encouraging mechanism to foster the character of the students majoring in nursing on loving their specialty,their strong professional knowledge,proficient operating skills and healthy mental quality.It aims at discussing the approaches on how to improve morals and culture of the students majoring in nursing in current situation.

Objective To probe into the chnical value of strain rate imaging on the assessment of left atrial function in patients with hypertensive disorder comphcating pregnancy(HDCP).Methods Thirty two patients with HDCP and 20 control subjects were involved.Patients with HDCP were divided into 2 groups:17 with normal left ventricular mass index (LVMI) and 15 with left ventricular hypertrophy.These patients and controls underwent transthoracic echocardiography,strain and strain rate imaging examination.Atrial strain and strain rate imaging values of patients with HDCP were compared with those of controls.Multiple parameters were compared by one-way anova analysis.Results When compared with the controls,peak early diastolic strain rate (ESR) was significantly decreased in HDCP patients with normal LVMI and left ventricular hypertophy [(-1.13 ± 0.15),(-1.04 ± 0.29) s-1 vs.(-1.26 ± 0.23) s-1,P ＜ 0.05].Compared with the normal LVMI,peak late diastolic strain rate (ASR) was significantly decreased in controls and patients with left ventricular hypertrophy [(-1.23 ± 0.25) s-1 vs.(-1.36 ± 0.33),(-1.34 ± 0.32) s-1].There was no significant difference in the others (P ＞ 0.05).Conclusion Left atrial function is decreased in HDCP patients,which with left ventricular hypertrophy,the tendency is more obvious.

Medical ethics teaching is the an important part of professional teaching for medical students. The development of economics and medical science will surely make the conception of medical ethics changing constantly, in return the formation of new medical ethics may improve the development of medical science. The society requires that the medical students of new century should become personals of high quality having both ethics and abilities and undertaking medical practice law-abiding. The teachers must renew their conception of medical ethics and pay attention to the infiltration of medical ethics in clinical teaching. The author will make a research through six aspects in the strengthening and infiltrating of medical ethics in clinical teaching so as to find the way of developing by the society qualified medical personals needed.

Objective To explore the clinical characters and the relationship between prognosis and treatment in childhood acute non-lymphocytic leukemia(ANLL).Method We summarized and analyzed the cases with acute non-lymphocytic leukemia in children patients from our hospital admission between Jan 2003 and Sep 2007.Results ①The M1,M2 subtype of childhood ANLL were about 37.1%;the clinical situation was various;extramedullary infiltration was not uncommon;②A totle complete remission(CR)rate was 68.6% and the CR rate of one course chemotherapy was 40.1%;③The CR rate of acute promyelocytic leukemia(APL)was 87.5% and a average of continues CR(CCR)was 33 months after the treatment of ATRA combined with As2O3;④Among five patients finished all the courses of high dose Ara-c(HDAra-c)intensification therapy,three were CCR over 36 months.Conclusion ①The CR rate of one course chemotherapy was correlated to the intensity of treatment;②The treatment of As2O3 can help to lower the relapse of APL and enhance the event-free survival(EFS);③The treatment of HDAra-c can help to increase the EFS rate of childhood ANLL.

The authors adopted an auto control experimental research on 18 male adult dogs with 36 sides of femoral heads and necks which were divided into two groups. They were separately made models of old fracture of femoral neck and avascular necrosis of famoral head. Two wecks later, The free fibula with microvascular an astomosis was tranplanted. All experimental dogs were examined with X-ray, pathohitology, electron microscopy, 99~mTc-MDP scanning and tetracycling labeling after operation.The experiment showed that the free fibular graft with mierovascular anastomosis could provided a new system of vascula supply to the injured femoral head. The hard free fibula and the metallic internal fixation were inserted in femoral head and neck. After the Operation, in the fracture area the fibula formed a bone-bridge which not only diminished the stress force but also prevented rotation displacement of the bone fracture The periosteum of the transplanted fibula also took part in the repair course of avascular nerosis of femoral head.

Objective To investigate the effect of Yishen-Daotan decoction on TCM syndrome elements of infertility caused by kidney deficiency and phlegm stagnant type polycystic ovary syndrome.Methods 60 patients of kidney deficiency and phlegm stagnant type polycystic ovarian syndrome infertilities were administrated with modified Yishen-Daotan decoction for 3 months and the changes of TCM syndrome elements,menstrual cycle and serum sex hormone were compared before and after the treatment.Results ① TCM syndrome elements had a statistical changes after the treatment compared with those before the treatment(t=62.79,P＜0.01).②menstrual cycle showed statistical difference after the treatment compared with that before the treatment,(x2=21.64,P＜0.01).③Serum FSH,E2 were increased,while LH,LH/FSH and T were decreased after the treatment,showing a significant difference compared with those before the treatment (t=2.67,P＜0.05).④The total effective rate was 91.67％ and pregnancy rate was 30％.Conclusion Yishen-Daotan decoction significantly improve clinical symptoms in patients with kidney deficiency and phlegm stagnant type PCOS infertility.

Objective To investigate the expression of neurotrophins receptor 75NTR in human breast cancer resistant cell line and its correlation with multidrug resistance. Methods Western blot was used to detect the expression level of p75NTR protein in various breast cancer cell lines and multidrug resistant cell lines. The over-expression vector and siRNA vector of p75NTR were constructed by gene recombination method. Western blot was used to detect the expression levels of p75NTR protein in transfected p75NTR and p75NTR - siRNA breast cancer resistant cell line MDA-MB-231/ADR;the sensitivity of MDA-MB-231 and MDA-MB-231/ADR to different chemotherapeutic anticancer drug (PTX ,ADM ,GEM ,DDP ,OXA) and the multi-drug resistance effects in over-expression and knock-down p75NTR MDA-MB-231/ADR were detected by CCK-8 assay. Results Western blot result showed that the expressions of p75NTR protein in the multidrug-resistant breast cancer cell lines MDA-MB-231/ADR and MCF-7/5-FU were higher than that of other breast cancer cell lines. Over-expression of p75NTRcan up-regulate the expression of p75NTR protein in MDA-MB-231/ADR;the CCK-8 assay indicated that over-expression of p75NTR can effectively enhance the resistance of MDA-MB-231/ADR cells to ADM,GEM and OXA. Conclusion The expression of p75NTR in breast cancer resistant cell line is higher than that of its parental cell line;over-expression of p75NTR can reduce the sensitivity to chemotherapeutic drugs and promote its multidrug resistance.

Objecfive To explore the value of endoscopic uhrasonography(EUS)in diagnosis and preoperative assessment of esophageal cancer.Methods A total of 57 patients with esophageal cancer were examined by EUS.The image features,depth of invasion and lymph node metastasis obtained by EUS were analyzed and compared with postoperative outcomes.Results Esophageal cancer was characterized as an irregular hypoeehoic mass with partial or total destruction of normal esophageal wall structure under EUS.Compared with post-operative pathological findings,the consistent rates of EUS diagnosis in depth of invasion,T staging,and N staging were 84.2%(48/57),87.7%(50/57)and 84.2%(48/57),respectively.Conclusion EUS exhibited high diagnostic accuracy in T staging of esophageal cancer,which is valuable in managements and prognosis of esophageal cancer.

Objective To study the effect of phosphodiesterase inhibitor pentoxifylline (PTX) on type 1 diabetes in NOD mice and its possible mechanism.Methods Blood glucose, urinary glucose, insulitis and incidence of diabetes were investigated in NOD mice treated with PTX, insulitis was observed by HE staining and the expressions of IFN ?, TNF ?, IL 10 in pancreas were measured by RT PCR technique. Results The incidence of diabetes in the PTX group was 30.0% which was significantly lower than 67.9% in the control group (P

Objective To investigate the infection of 23 kinds of human papillomavirus ( HPV) subtypes in female cervical epithelium samples and to analyze their relationships with age and results of cyto-logic test in Haikou area. Methods A total of 4 037 local healthy women were enrolled in this study from July 2013 to August 2016, 1 967 of whom received cervical cytology test. Cervical cell samples collected from those women were detected for HPV typing by using PCR-reverse dot blot hybridization. Results (1) The total positive rate of HPV in 4 037 samples was 22. 15% (894 cases), and the detection rates of carci-nogenic, possibly carcinogenic and non-carcinogenic HPV were 16. 13%, 3. 99% and 5. 55% (651, 161 and 224 cases), respectively. The positive rates of 6 genotypes were high, which were HPV52, 53, 81, 51, 16 and 58 in turn. (2) The detection rates of carcinogenic, possibly carcinogenic HPV and some of the gen-otypes (HPV18, 52, 53, 66) increased with age (all P<0. 05). (3) Multiple infection in HPV-positive women accounted for 24. 38% (218/894), in which the infection rates of carcinogenic types declined with age and the numbers of HPV genotypes in carcinogenic infections were negatively correlated with age ( both P<0. 05). (4) Only 2. 49% (49/1 967) of the samples were positive for cervical cytologic test, classified into the ≥ASC-US ( atypical squamous cells of undetermined significance ) group. The detection rates of eight kinds of carcinogenic and two kinds of possibly carcinogenic HPV in≥ASC-US group were significantly higher than those in the negative (no intraepithelial lesion or malignant lesion, NILM) group (all P<0. 05). Conclusion This study indicates that Haikou women have higher rates of HPV infection, especially the eld-erly group. HPV subtype infections present some regional characteristics and are mainly single type infec-tion. Cervical cancer screening should be combined with tests for HPV and cytology analysis to improve its effectiveness.