Acupuncture Therapy ; Adult ; Aged ; Combined Modality Therapy ; Female ; Herpes Zoster ; therapy ; Humans ; Male ; Middle Aged ; Moxibustion ; Treatment Outcome ; Young Adult

OBJECTIVE:To observe the effect of compound glycyrrhizin on patients with cholelithes combined with ob?structive jaundice.METHODS:The changes of bile drainage and liver function in41patients with choledocholith and obstruc?tive jaundice were observed on1to15days after operation(the trial group).The control group did not receive compound gly?cyrrhizin.RESULTS:The bile drainage of the trial group arrived to stable state3days earlier than that of the control group and color of bile was darker than that of control group;The liver function of trial group was significantly better than that of the control group from the7th day after operation(P

Objective:To explore influence of diltiazem hydrochloride on expression of serum microRNA in aged pa‐tients with dilated cardiomyopathy (DCM ) . Methods :A total of 120 aged DCM patients (DCM group ) and 60 healthy aged people (normal control group) were enrolled .DCM group was further randomly and equally divided in‐to routine treatment group (n=60) and diltiazem group (n=60 ,received additional diltiazem hydrochloride based on routine treatment) .Left ventricular end -diastolic dimension (LVEDd) ,left ventricular posterior wall thickness (LVPWT) and left ventricular ejection fraction (LVEF) were observed and compared between routine treatment group and diltiazem group before and after treatment ,cardiac function improvement was compared between these two groups after treatment .Changes of expression amount of serum miR -1 ,-133 ,-499 and -208 were com‐pared among three groups before ,six and 12 weeks after treatment .Results:Compared with before treatment ,after treatment ,there were no significant changes in LVEDd ,LVPWT and LVEF in routine treatment group , P>0.05 all;there were significant reductions in LVEDd [(69.41 ± 6.42) mm vs .(61.32 ± 5.31) mm] and LVPWT [(20.98 ± 1.98) mm vs .(12.05 ± 2.42) mm] and significant rise in LVEF [(32.81 ± 4.71)% vs .(50.52 ± 6.88)% ] in dilt‐iazem group , and LVEDd , LVPWT were significantly lower than those of routine treatment group [LVEDd:(67.25 ± 4.21) mm ,LVPWT :(18.49 ± 2.86) mm] ,and LVEF was significantly higher than that of routine treat‐ment group [ (47.77 ± 3.92)% ] , P<0.05 all .For cardiac function improvement ,markedly effective rate (71.7%vs .56.7% ) and total effective rate (98.3% vs .85.0% ) of diltiazem group were significantly higher than those of routine treatment group , P<0.05 both . Compared with normal control group , the 4 kinds of miR expression sig‐nificantly reduced in routine treatment group , and compared with routine treatment group , those of diltiazem group were significantly rose , P<0.01 all , but compared with 6‐week , miR expression had downtrend during 12‐week , the miR‐208 expression was most significant ( P< 0.01 ) .Conclusion:Diltiazem could effectively modulate serum microRNA expression in aged CDM patients ,which provided molecular biological basis for studying diltiazem mech‐anism .

BACKGROUND:The sensitivity andmucus secretion of theoral mucosamake oral soft tissues difficult to repair, so patients cannot achieve satisfactory outcomes after treatment. Nano-celulose protein mainly composed of glycine, alanine and serine has good histocompati bility. However, there is a lack of comparative study about the effect of nano-celulose protein and acelular matrix in oral mucosa repair, and the clinical effects of the two materials are stil under discussion.
OBJECTIVE:To investigate the effects of nano-celulose proteinver susacelular matrix in oral mucosa repair.
METHODS:Oral mucosadefect models were preparedinrats, and these rat models were randomly divided into four groups:oral mucosa defectswere repaired by vaseline (control group), nano-celulose protein, bovine skin acelular matrix and human skin acelular matrix, respectively. Repair effects were compared among different materials within 2 months after surgery.
RESULTS AND CONCLUSION:The diameter of oral mucosa defect measured using a vernier caliper, had no significant differences among groups at 1 day after surgery (P> 0.05); the diameter of oral mucosa defect in the nano-celulose protein group was significantly lower than that in the other groups at 3, 5 and 7 days after surgery (P<0.05); the diameter of oral mucosa defect in the bovine and human skin acelular matrix groups was significantly lower than that in the control group at 5 and 7 days after surgery (P< 0.05). Morphological observationoftheoral mucosa under light microscope showed: the number of newborn capilary endothelialcels in the defect region had no significant differences among nano-celulose protein, bovine acelular matrix and human acelular matrixgroupsat 1, 3, 5 and 7 weeks after surgery (P> 0.05);but there were significant differences in the number of newborn capilary endothelialcels between the control group and the other three groups (P< 0.05). Furthermore, at 21 days after surgery, closely aligned and thicker new epithelialtissuecould be found in the nano-celulose protein group; in the bovine acelular matrix group,thedefect regionwasrepaired wel, new epithelialtissueappeared andthe number of inflammatory cels decreased; in the human acelular matrix group, inflammatory cels appearedobviously, and new epithelialtissueformed with the normal thickness. In contrast,abundant inflammatory cels and thinner epithelial tissues appeared in the control group. To conclude,bothnano-celulose protein and acelular matrix can accelerate wound healing by promoting oral mucosal epithelial hyperplasia.

BACKGROUND:Related medical researches have shown that the incidence of simple diastolic heart failure is higher than that of systolic heart failure. OBJECTIVE:To investigate the application value of echocardiography in the monitoring of rabbit models of simple diastolic heart failure. METHODS: Sixty healthy male New Zealand rabbits were included in this study. The pressure overload hypertrophy rabbit model was established by the method of abdominal aortic coarctation. Rabbits were randomly divided into surgical group and sham group. Al rabbits were subjected to the echocardiography and left heart catheterization, the left ventricular end-diastolic pressure and relaxation time constants were measured to judge the presence of simple diastolic heart failure. RESULTS AND CONCLUSION:Compared with the sham group, the aortic annular diameter was significantly reduced before surgery and at 1, 4, 8 weeks after surgery in the model group (P < 0.05); the peak of retrograde velocity at atrial contraction was decreased in the model rabbits at 8 weeks after surgery (P< 0.05); the left ventricle dry weight, ratio of left ventricle dry weight/body weight, left ventricular posterior wal thickness, systolic pressure, interventricular septum, left ventricle systolic pressure, and left ventricular end-diastolic pressure were obviously increased at 8 weeks after surgery (P < 0.05). Experimental findings indicate that rabbit models of simple diastolic heart failure are successfuly established, and echocardiography can effectively monitor the left ventricular systolic and diastolic functions in cardiac hypertrophy.

BACKGROUND:Fibroin is a natural macromolecular material with Arg-Gly-Asp peptide structure that is a special tripeptide structure closely related to cel adhesion, and it can promote cel migration, adhesion, and proliferation and influence cel morphology and function. OBJECTIVE:To compare the effects of different silk fibroin scaffolds to repair buccal mucosa defects in rats. METHODS:Ninety Sprague-Dawley rats were selected to make unilateral buccal mucosa defect models, and randomly divided into three groups, 30 rats in each group: porous silk fibroin scaffold was implanted into the buccal mucosa defect in experimental group, multi-layered crosslinked silk fibroin film was implanted into the buccal mucosa defect in control group, and vaseline gauze was used to cover the buccal mucosa defect folowed by suturing in blank control group. After 15 days, wound diameter was detected; after 30 days, bone defect tissues were taken for hematoxylin-eosin staining. RESULTS AND CONCLUSION:At postoperative 15 days, the wound diameter was significantly smaler in the experimental group than the control and blank control groups (P < 0.05), as wel as smaler in the control group than the blank control group (P < 0.05). Hematoxylin-eosin staining showed that at 30 days after operation, there were more epithelial spikes and fibroblasts, but less inflammatory cels in the experimental group than the other two groups (P < 0.05), and fibroin fibers were partialy absorbed and degraded in the experimental group. These findings indicate that porous silk fibroin scaffold for buccal mucosa defect repair can accelerate epithelialization and wound healing.

Objective To study the real?time dose verification with 2D array ion chamber array in volumetric modulated arc therapy ( VMAT) with a 2D array ion chamber array. Methods The 2D ion chamber array was fixed on the panel of electronic portal imaging device (EPID). Source?detector distance (SDD) was 140 cm. 8 mm RW3 solid water was added to the 2D array to improve the signal noise ratio. Patient plans for esophageal, prostate and liver cancers were selected to be delivered on the cylindrical Cheese phantom 5 times in order to validate the reproducibility of doses. Real?time patient transit dose measurements were performed at each fraction. Dose distributions were evaluated using gamma index criteria of 3 mm DTA and 3% dose difference referred to the first time result. Results The gamma index pass rate in the Cheese phantom were about 98%;the gamma index pass rate for esophageal, prostate and liver cancer patient were about 92%, 92% and 94%, respectively. Gamma pass rate for all single fraction were more than 90%. Conclusions The 2D array is capable of monitoring the real time transit doses during VMAT delivery. It is helpful to improve the treatment accuracy.

Objective:To consolidate ten years of clinical experience on the application of breast fiberoptic ductoscopy (FDS) in breast intra-ductal lesion. Methods:The clinical data of 1 368 cases of patients with nipple discharge were retrospectively collected and analyzed. Results:Significant differences were observed in the FDS diagnoses of patients with nipple discharge. The rates of tumor de-tection by FDS diagnosis were significantly higher when bloody and serous nipple discharge was used rather than milky and watery nip-ple discharge. For non-tumor nipple discharge, local drug perfusion via FDS was an effective treatment. A total of 303 patients had tu-mor resection or segmentectomy under localization via FDS, and 44 had segmentectomy after breast duct infusion of methylene blue. The diagnostic rate of localization via FDS (97.0%) was higher than that of breast duct infusion of methylene blue (86.4%). Conclu-sion:FDS is an accurate method for diagnosing patients with nipple discharge. In addition, it is also a good local drug perfusion method for patients with breast inflammatory nipple discharge ductoscopy. For patients with tumorous nipple discharge, localization via FDS can help improve the detection of the lesions, which can be removed by surgery.

Mesenchymal stem cells (MSCs) have good immune regulatory function,can inhibit many immune cell proliferation,direct effects on activation and proliferation of T cell,play a role in immune regulation by Treg cells or by the secretion of soluble factors regulating Thl/Th2 secretion and reaction equilibrium,inhibit the inflammation through the anti-inflammatory,regulation of cytokines expression at the same time,so reduce the expression levels of matrix metalloproteinase-2 and-9,which may promote lacrimal gland tissue damage,and thus play a role in immune regulation.MSCs can reduce the autoimmune dry eye clinical index,recovery secretion function of part lacrimal gland.This article reviews the research advances in inmmmne regulation of MSCs on autoimmune dry eye.

This article was aimed to study the characteristics of intestinal cancer by traditional Chinese medicine (TCM) based on the scale-free network analysis method. History information of 145 hospitalized cases from 2008 to 2011 was collected from the Oncology Department, Guang A nmen Hospital, in the clinical collection system. The rule of medication in the treatment of intestinal cancer by TCM was explored by scale-free network analysis method from several aspects, such as effects, classification and compatibility relations. The analysis results of 145 intestinal cancer cases showed that strengthening the body resistance was the main treatment principle. Si-Jun-Zi (SJZ) decoc-tion was used with the highest frequency. In the prescription design, qi-supplementing herbs were the most. The heat-clearing, dampness-eliminating, qi-regulating, blood-activating and stasis-removing, food stagnation removing and phlegm-removing herbs were also used frequently. The TCM treatment and syndrome differentiation will be changed due to the treatment and disease stages. It was concluded that scale-free network analysis method is able to relatively show the TCM treatment rule of intestinal cancer quantificationally and intuitively.