Objective Previons studies showed that,the gene of P16 is a kind of gene suppressing the cancer.its function once lose,the cell may change to cancer.Methods The expression of P16 has a closely relation with the malignant latent and evolve of the oesophagus cancer.ResulIs This research explored the relation between the P16 expression and the prognosis of oesophagus cancer,the results showed the positive rate of the expression of P16 is more low the rate ofexistence is more low,it in dicates the expression rate of P16 can reflect the prognosis of oesophagus cancer cell.Conclusion The expression of P16 missing or descending is closely rehted with the aggravate and evolve of oesophagus cancer,therefore the expression of P16 had a relation with the prognosis of oesophagus cancer.

Hyperuricemia is closely associated with abdominal obesity .The prevalence of hyperuricemia combined with abdominal obesity has been increased significantly in recent years , along with the improvement of daily life and the changes in dietary structure .The state of hyperuricemia combined with abdominal obesity is most harmful , and becomes a common and high risk metabolic disease .Animal model with hyperuricemia combined with abdominal obesity is very impor-tant for the research of pathomechanism and treatment of this disease .

Objective To explore the therapeutic effect of Cefoselis sulfate and Cefepime on the stroke-associated pneumonia in elderly patients.Methods 96 cases of stroke-associated pneumonia with positive pathogen were divided into two groups treated with Cefoselis versus Cefepime respectively.Body temperature,leukocyte count,arterial partial oxygen pressure,the serum level of C-reactive protein (CRP) and bacterial clearance rate were observed before and after the treatment.The cefoselis sulfate group (n =48) or the cefepime group (n =48) was treated with 2.0 g Cefoselis sulfate or 2.0 g Cefepime every 12 hours for 7 to 10 days.Results In post-treatment versus pretreatment,both two groups showed that clinical symptoms and inflammatory indicators were improved,and the patient's body temperature,white blood cell count and neutrophil cell count,hs-CRP and arterial partial oxygen pressure were significantly decreased (all P＜0.05).Arterial partial oxygen pressure was better in post-versus pre-treatment (P＜0.05).The total effective rate was 87.5％ in Cefoselis sulfate group and 83.3％ in cefepime group (P ＞ 0.05).The bacterial clearance rate was higher (89.6％) in Cefoselis sulfate groups than in cefepime groups (67.9％) (P＜0.05).In posttreatments of Cefoselis sulfate groups versus Cefepime groups,white blood cell count and neutrophil cell count,hs-CRP and arterial partial oxygen pressure showed no statistically significant differences (all P ＞ 0.05).Conclusions Compared with Cefepime,Cefoselis has the similar anti-infection efficacy in the treatment of stroke-associated pneumonia.While,the bacterial clearance rate and the total effective rate are better in cefoselis group than in Cefepime group.

Two Pseudomonas strains, K80 - B and K80 - A, can use hydrocarbon as carbon source by two different mechanisms. Different effects can be observed when they are applied to 69-8 oil of Dagan Oil Field. The strain K80-B producing both hydrocarbon pseudosolubilizing factor and hydrocarbon emulsifying factor can decrease the viscosity and solidifying point of the 69-8 oil 51.6% and 2℃ respectively. Whereas the other can only decrease 43.5% and 3℃ respectively. When the two strains are mixed, synergistic effect on crude oil is obvious.

Objective:To re-evaluate the quality of methodology and outcome indicators for systematic reviews/meta-analysis about the effectiveness of non-pharmacological interventions for chemotherapy-related nausea and vomiting(CINV).Methods:The Cochrane Library, PubMed, Embase, Web of Science, CNKI, Wanfang, VIP and CBM for systematic reviews/meta-analysis on the effectiveness of non-pharmaceutical intervention in the prevention or treatment of CINV from inception to May 2021 were searched. The methodological quality of the included literature was evaluated by the AMSTAR 2 quality evaluation tool, and the quality of the evidence for the outcome indicators was evaluated by GRADE system.Results:A total of 24 articles were included, 7 of the AMSTAR 2 quality evaluation results were low-level, and the remaining 17 were all very low-level. The main defects were the lack of preliminary study design scheme, incomplete search strategy, no list of excluded literature, and no report of included research funding sources, etc. Only 1 of the outcome indicators was classified as high quality, 7 were classified as intermediate, and the rest were low or very low quality.Conclusions:Methodological quality and strength of evidence of systematic reviews/meta-analysis on the effectiveness of non-pharmaceutical intervention for CINV are generally low, and the reliability of research results is poor. It is necessary to design scientific and rigorous high-quality RCTs and systematic reviews to further verify the effectiveness of non-pharmaceutical interventions in the future.

Objective:To retrieve, appraise and summarize the best evidence to prevent accidental extubation of peripheral venous catheters in adults.Methods:According to the "6S" evidence model, computer evidence retrieval was carried out. Search relevant domestic and foreign guideline networks and databases to collect relevant evidences, including clinical decision, guidelines, evidence summary, systematic reviews, etc. The retrieval time was from the establishment of the database to September 2021. Two researchers conducted independent literature search, quality evaluation, evidence extraction and summary. If there is a disagreement between the two, the third party shall be invited to make a ruling.Result:A total of 8 articles were included, including 1 clinical decision, 1 guideline, 2 evidence summaries, 3 RCTs and 1 expert consensus. The 35 best evidences were summarized from six aspects: education and training, catheter placement selection, dressing selection, catheter fixation, catheter maintenance and timing of extubation.Conclusion:This summary of evidence provided evidence-based evidence for the standardized management of clinical prevention of accidental extubation of peripheral venous catheters. However, some of evidence is lacking and of poor quality. In the future, the evidence should be used cautiously according to the clinical situation and patient conditions.

Objective:To search, evaluate and summarize the best evidence for non-pharmaceutical therapy of cancer-related fatigue in cancer patients.Methods:According to the "6S" evidence model, systematically searched relevant domestic and foreign guideline networks and databases to collect relevant evidences, including clinical decision support, guidelines, evidence summaries, and systematic reviews. Retrieval time from the establishment of the database to August 2021. After evaluating the quality of the literature, we extracted and summarized relevant evidence.Results:A total of 18 articles were included in this study, including 1 clinical decision support, 4 clinical practice guidelines, 5 evidence summarie and 8 systematic reviews. 25 pieces of best evidence were summarized, involving non-pharmacological interventions in 7 aspects: health education, exercise intervention, psychosocial intervention, traditional Chinese medicine therapy, nutritional support, sleep therapy and bright white light therapy.Conclusions:It is recommended that medical staff should combine clinical practice, scientifically select the best evidence and use evidence-based management scheme for cancer-related fatigue to reduce cancer-related fatigue and improve patients′ life quality.

Objective To explore the value of short from health survey-36 (SF-36) in evaluating quality of life and pain care in patients with cervicogenic headache treated by coblation.Methods Thirty patients with cervicogenic headache accepted coblation treatment in our hospital from February 2017 to August 2018 were chosen in this retrospective analysis. The changes of visual analogue scale (VAS) scores, self-rating anxiety scale (SAS) scores and SF-36 scores (quality of life) before and after treatment were analyzed, and the correlations of these scale scores were determined by Pearson correlation analysis .Results After the 10 weeks of follow-up, the VAS scores of these 30 patients decreased from 7.70±0.88 to 3.30±1.12, SAS scores decreased from 62.47±4.59 to 49.20±6.48, and SF-36 scores increased from 38.50±4.15 to 78.64±6.39, with significant differences (P<0.05). VAS scores were positively correlated with SAS scores (r=0.720,P=0.012), but negatively correlated with SF-36 scores (r=-0.850,P=0.001); SAS scores were negatively correlated with SF-36 scores (r=-0.940,P=0.000). Conclusion SF-36 can be used as a reference for pain nursing intervention in patients with cervicogenic headache after coblation.

Objective To investigate the clinical manifestations,molecular genetics and gonadal pathol-ogy characteristics of patients with disorders of sex development(DSD),and to summarize the clinical experi-ence of identifying rare diseases from common symptoms.Methods The clinical data of 416 patients with DSD diagnosed and treated in the multidisciplinary center of DSD of Guangzhou Women and Children's Medical Cen-ter from May 2018 to August 2023 were retrospectively analyzed,summarized and discussed.Results Accord-ing to chromosome karyotype,416 cases of DSD were classified into three types:92 cases(22.1％)of abnormal sex chromosome karyotype,285 cases(68.5％)of 46,XY karyotype and 39 cases(9.4％)of 46,XX karyotype.Among the 92 patients with abnormal sex chromosome karyotype,59 cases were raised as males,18 cases(30.5％)complained of short penis with hypospadias and cryptorchidism.The most common karyotype was 45,X/46,XY(58 cases,63.0％).Among the 285 patients with 46,XY karyotype,238 cases were raised as males,and 63 cases(26.5％)complained of short penis and hypospadias;47 cases were raised as females,and 13 ca-ses(27.7％)complained of inguinal mass.A total of 216 patients with 46,XY karyotype were subjected to whole exome gene detection,and 155 cases(71.8％)were found to have molecular pathogenesis with the clinical phe-notype.Among the 39 patients with 46,XX karyotype,19 cases were raised as males,and 8 cases(42.1％)com-plained of short penis and hypospadias.In the 18 cases of gonad biopsy,17 cases showed testicular tissue in go-nads.Whole exome sequencing was performed in 14 cases.NR5A1 gene heterozygous mutation,SRY gene muta-tion and SOX3 gene mutation were found in 2 cases,respectively(14.3％).Twenty cases were raised as females,and 14 cases(70.0％)complained of clitoral hypertrophy.Gonad biopsy was performed in 8 cases,with 7 cases of ovotestis(87.5％)and 1 case of NR5A1 gene heterozygous mutation(14.3％).Conclusions The etiologies of DSD are complex and diverse,and the clinical manifestations are various,which can be manifested as hypospa-dias,micropenis,cryptorchidism and other common symptoms of the urinary system.Different etiologies have dif-ferent treatment options.Therefore,chromosome karyotype,molecular genetic testing and gonadal pathology can be used to clarify the cause of disease,especially for rare diseases,improve the detection rate,reduce the rate of missed diagnosis,and ensure reasonable treatment,especially sex selection.

Objective:To design and implement a network-based quality management system for tumor radiotherapy.Methods:The system consists of B/S framework-based three-layer structures including the application layer, system service layer and data layer. It utilizes Nutz as the development framework to develop web applications, MySQL as the system database, Java programming language for system development, Tomcat as a system application server for project release and IE, Google and other mainstream browsers to achieve client access server functions.Results:The system can support integrated information management and service of quality control institutions at the provincial, municipal and county levels. The functions include procedure management, case management, quality control management, notification announcement, data management and system management, etc. The system has been set up and tested in the cooperation units, and the operation and function are in good condition.Conclusion:The system can support the assessment of online quality control, which is conducive to information analysis and sharing, promotes the standardization and normalization of quality control and improves work efficiency. Case management function can monitor the diagnosis and treatment processes of patients, establish continuous electronic record, deliver rational treatment and rehabilitation guidance plan, which play a pivotal role in the evaluation of tumor radiotherapy.