Objective To investigate effect of leptin on nerve function in patient with Parkinson disease .Methods 68 cases patients with PD were selected and randomly divided into two groups, according to random number table, from October 2013 to December 2014 in the hospital.34 cases in control group were treated with conventional therapy, and 34 cases in experimental group were treated with leptin, with a consecutive treatment of twelve weeks.The score of activity of daily living scale ( ADL ) and mini-mental state examination ( MMSE ) were analyzed pre and post-treatment. ResuIts After treatment, the ADL score of experimental group was significantly lower than that of control group ( P<0.05 ) , MMSE score of experimental group was significantly higher than that of control group (P<0.05).The adverse reactions in both groups were minor.ConcIusion Leptin has good curative effect in treatment of Parkinson disease, and could significantly improve the neurological function of patients, with mild adverse reactions.

Objective To identify the outcomes and effect of applying the health check-up nursing quality control system developed as supervised by JCI quality assessment standards.Methods The monitoring system for nursing quality in the health check-up department is established within the framework of the hospital quality improvement committee and in line with characteristics of the department.Quality improvement tools may be called into play for analysis and decision making to revolve critical problems found in health check-up nursing,upgrading nursing quality and customer satisfaction.Results Significant rise of health check-up quality and customer satisfaction for nurses,and the nurses are trained in quality control knowledge and get further involved in quality management of their department.Conclusion Health check-up nursing management system under the JCI standard is conducive to raising the nursing quality,and helps nurses with problem analysis and solution.

Objective To study the clinical features and treatment of cervical spondylosis with dissociated motor loss in upper extremity. Methods Data analysis was done in 10 patients with cervical spondylosis with dissociated motor loss in upper extremity, in aspects of symptoms, sign, radiologic feature, electromyogram and treatment. Results There are 2 subtypes of cervical spondylosis with dissociated motor loss in upper extremity: Keegan and Non-Keegan types. The patients with Keegan type of cervical spondylosis manifested weakness and atrophy in the upper extremity, and no radicular pain was reported. The Non-Keegan type is in fact a combination of radicular and spinal insult in the cervical region. Our clinical practice showed that surgical operation was preferred to the conservative treatment for patients with this disease. Conclusion A well understanding of this disease is of great importance for avoiding mistakes in diagnosis and treatment of this disease.

Job-transfer training for general practitioner is the important channel of primary medical talents team construction.This article elaborated on existing problems in the general practitioner job-transfer training in our country from several aspects,such as training base,teachers,teaching materials,actual work need and discipline construction,etc.Based on the existing problems and combined with practical work requirements of general practitioner job-transfer training in our country,the article put forward corresponding suggestions and countermeasures including strengthening teachers' construction,textbook construction and speeding up general medical discipline construction,etc.

Objective: To observe antipyretic, diuresis, anti inflammatory and antisepsis effects of Qinglin Granules. (Herba Polygoni Avicularis, Herba Dianthi, Caulis Aristolochiae Manshuriensis, Semen Plantaginis, Fructus Gardeniae, etc.) Methods: The model of pyretic rats induced by yeast was used to prove antipyretic action. The diuresis action was observed by the test. The anti inflammatory effect was observed by pettitoes swelling in rats and auricle swelling in mice. The paper diffuse methods were used to confirm the antisepsis effect. Results: Qinglin Granules not only had the obvious effect of relieving fever and increasing the amount of urine, but also had the strong inhibition on the two kinds of inflammatory models, moreover it can widen the diameter of the antisepsis ring which showed it had the inhibition on cdibacillus, pseudomonas, aeruginosa, bacillus gasoformans, staphylococcus aureus and gonococcic. Conclusion: Qinglin Granules have the obvious antipyretic, diuresis, anti inflammatory and antisepsis effects.

As the main medicinal powder for drawing out pus and removing necrotic tissue in external therapies of traditional Chinese surgery, Sheng Powder has made great contributions to the treatment of inflammatory wounds and has the unique bactericidal and decay-discharging function that can not be replaced by antibiotics. However, Sheng Powder has toxicity because it contains mercury. So far, there is no clinical research on the standards of dose and usage of Sheng Powder and there is a lack of objective and quantitative criteria for operating standards and monitoring of toxicity and side effects. Therefore, the authors choose Jiuyi Powder, one of the most commonly used Sheng Powder, to evaluate the safety of its external use, and form a standardization program for clinical implementation.

Objective To compare the effects of hemodialysis (HD) and peritoneal dialysis (PD) in the treatment of elderly patients with end-stage renal disease.Methods The clinical data of 100 elderly patients with end-stage renal disease who were treated in the People's Hospital of Beilun District from March 2013 to April 2015 were retrospectively analyzed.The patients were divided into HD group and PD group according to the treatment methods,with 50cases in each group.The 2-year survival rate of the patients in the two groups was observed,and the differences of renal function,nutritional index and lipid metabolism were compared between the two groups before and after treatment.Results Before treatment,there were no statistically significant differences between the two groups in the levels of proinflammatory cytokines.After treatment,the levels of IL-18 [(56.22 ± 7.32) mg/L],IL-6 [(12.18 ± 2.03)mg/L] and hs-CRP[(8.54 ± 1.12)mg/L] in the HD group were lower than those in the PD group [(78.46 ± 7.28) mg/L,(15.23 ± 2.25) mg/L,(10.23 ± 1.67) mg/L] (t =15.233,7.117,5.943,all P ＜ 0.001).After treatment,the levels of BUN [(6.22 ± 1.05) mmol/L],SCr[(115.44 ± 10.84) μmol/L] in the HD group were lower than those in the PD group [(8.87 ± 1.22) mmol/L,(134.52 ± 15.23) μmol/L] (t =5.943,7.217,all P ＜ 0.001),the GFR[(8.15 ± 13.35)mL/min] of the HD group was higher than that of the PD group [(9.26 ± 12.58) mL/min] (t =-4.969,P ＜ 0.001).Before treatment,the nutrition indicators between two groups had no statistically significant difference.After treatment,the levels of ALB [(53.62 ± 4.13)g/L],PA[(342.15 ± 15.02) mg/L] and Hb [(115.86 ± 10.02)g/L] in the HD group were higher than those in the PD group [(45.95 ± 5.57) g/L,(228.36 ± 12.23) mg/L,(98.02 ± 7.12) g/L] (t =-7.821,-41.541,-10.263,all P ＜ 0.001).Before treatment,the lipid metabolism indicators between the two groups had no statistically significant differences(all P＞0.05).After treatment,the levels of TC[(4.78 ±0.98) mmol/L],LDL[(2.51 ±0.97) mmol/L] in the HD group were lower than those in the PD group [(5.53 ± 1.02) mmol/L,(2.89 ± 1.03) mmol/L] (t =3.749,1.899,all P ＜ 0.05),the level of HDL [(1.39 ± 0.46) mmol/L] of the HD group was higher than that of the PD group [(1.22 ± 0.34) mmol/L] (t =-2.101,P ＜ 0.001).The 2-year survival rate in the HD group was 96.00％,and that in the PD group was 86.00％.There was no statistically significant difference between the two groups in the 2-year survival rate (P ＞ 0.05).Conclusion HD has better therapeutic effect on elderly patients with end-stage renal disease,it can significantly improve the metabolic level of patients and improve the survival rate of patients.

Objective To investigate the clinical efficacy of alprostadil in the treatment of diabetic nephropathy,and its effect on serum bone morphogenetic protein 7(BMP-7)and transforming growth factor-β1(TGF-β1)levels in patients.Methods From January 2014 to January 2016,120 cases of diabetic nephropathy in the People's Hospital of Beilun District were selected in the study.According to different treatment methods,the patients were divided into study group and control group,with 60 cases in each group.The two groups were treated with basic treatment,the study group was treated with alprostadil for 8 weeks.The clinical efficacy and serum levels of BMP-7 and TGF-β1 were compared between the two groups.Results There were no significant differences in serum BMP-7 and TGF-β1 between the two groups before treatment(P>0.05).After treatment,the serum BMP-7[(17.54 ±3.90)pg/mL]in the study group was higher than that in the control group [(15.20 ±2.96)pg/mL](t=3.607,P<0.05),the level of TGF-β1[(7786.3 ±1951.2)pg/mL]was lower than that of the control group [(10021.5 ±2109.7)pg/mL](t=6.025,P<0.05).There were no significant differences in the levels of fasting blood glucose(FBG),total cholesterol(TC),triglyceride(TG)and glycosylated hemoglobin(HbA1c)between the two groups(all P >0.05).After treatment,the levels of UAER,Hcy,Scr,BUN in the study group were(89.62 ±17.74)g/min,(23.55 ±4.17)mol/L,(64.2 ±8.5)mol/L,(6.70 ±0.96)mmol/L,which were significantly lower than those in the control group [(118.50 ± 21.18)g/min,(29.69 ±4.82)mol/L,(74.7 ±9)mol/L,(7.52 ±0.89)mmol/L](t=8.097,7.462,6.57,4.852,all P<0.05).Conclusion Alprostadil has better clinical effect on diabetic nephropathy,and can significantly improve serum BMP-7 and TGF-β1 levels.

Objective To construct the eukaryotic expression vector of cyclin-dependent kinase ( CDK) 7 labeled with Myc tag, obtain the expressed product , and identify its interaction with FLAG-P53 at the protein level .Methods Human CDK7 coding gene region amplified from the mammary cDNA library by PCR was inserted into the pXJ -40 vector.The recombinant plasmid Myc-CDK7 transfected into human 293T cell lines was investigated and examined by SDS-PAGE and Western blotting.In addition,assay was applied to determine the interaction between Myc-CDK7 and FLAG-p53.Results The coding region of CDK7 was successfully amplified by PCR and cloned into pXJ-40 vector, which was identified by double enzyme digestion and gene sequencing .Myc-CDK7 was successfully expressed in human 293T cell lines according to SDS-PAGE and Western blotting assay indicated that Myc-CDK7 could interact with P53 protein, which verified its known function .Conclusion The eukaryotic expression vector Myc-CDK7 is successfully obtained , which will contribute to further research on CDK 7-mediated cell cycle regulation .

Objective To construct the prokaryotic expression vector of human autophagy-related LC3B gene,obtain the GST-LC3B recombinant plasmid , purify the GST-LC3B fusion protein and identify its activity in vitro.Methods Human LC3B coding region was amplified from the human mammary gland cDNA by PCR and inserted into the prokaryotic expres -sion vector pGEX-KG.The recombinant plasmid pGEX-KG-LC3B was transformed into E.coli Rossate.The expressed product was purified by GST-Sepharose 4B beads and identified by SDS-PAGE and Western blot analysis .The function of the purified protein GST-LC3B was detected by GST pull-down assay.Results About 400 bp of the LC3B coding region was successfully amplified from the mammary gland library by PCR and inserted into pGEX -KG.The result of double diges-tion and sequencing showed that the GST-LC3B recombinant plasmid was successfully obtained .The GST-LC3B fusion pro-tein of about 40 000 (Mr) was successfully purified and identified by SDS-PAGE and Western blotting analysis.GST pull-down assay showed that GST-LC3B could interact with Atg4B, which identified its known function .Conclusion The pro-karyotic expression vector of GST-LC3B is constructed successfully , which will facilitate further research on the function of LC3B in autophagy.