OBJECTIVE:To study the preparation technique for CMC-Na mucilage.METHODS:The factors including kinds of solvents,the prescription dosage of CMC-Na and its adding way,the preferable prescription of and preparation for the above were inspected using orthogonal test of3factors and levels.RESULTS:The preferable technique was that2.5%CMC-Na was spreaded into the hot purified water solution under its stirring.CONCLUSIONS:The prescription and preparation for CMC-Na mucilage selected by the orthogonal test is consistent with Chinese Hospital's Preparation Standards.

Objective: To assess the tissue reactions of dogs to a new nanohydroxyapatite. Methods:Three dogs were used for root canal filling experiment, mandibular implanting and subcutaneous implanting experiment. AH-plus root canal sealer was used as control. The dogs were killed after implantation for 2,4 and 12 weeks separately. The tissue reaction was assessed by X-ray and light microscope examination. Results:①Root canal filling experiment: no inflammatory reaction was detected in specimens of n-HA group. In AH-plus group, implantation caused a middling inflammatory reaction after 2 weeks and 4 weeks. After 12 weeks the reaction of apical tissues was slight. ②Mandibular implanting experiment: no inflammatory reaction was showed by all n-HA groups and the group of AH-plus implanted for 12 weeks.Mild inflammatory reaction was observed in the groups of AH-plus implanted for 2 weeks and 4 weeks.③ Subcutaneous implanting experiment:after AH-plus was implanted for 2 weeks and 4 weeks, middling inflammatory reaction was observed. The group of n-HA implanted for 2 weeks showed the same reaction. No obvious inflammatory reaction was found in the other groups. Conclusion:n-HA shows better tissue compatibility than AH-plus.

AIM: To observe the effects and location of autologous bone marrow stem cells(BMSCs) transplanted through renal artery into ischemic-reperfusion(I/R) injured kidney.METHODS: BMSCs were collected from rabbits after isolated and then labeled with 5-bromo-2-deoxyuridine(BrdU).Twenty-eight rabbits were subjected to clamping renal pedicles for 105 min and divided into the transplantation group and control group randomly.BrdU labeled BMSCs or saline were injected into the kidney by renal artery,respectively.Before and after I/R at the 1st,3rd, 5th,7th,14th,21th and 28th d,the venous blood was collected to measure serum Cr and BUN.In the same time,renal tissue was collected for pathological and immunohistochemical study.RESULTS: After I/R,serum Cr and BUN levels in the rabbits in two groups became higher,and on the 1st and 3rd d after I/R,reached the highest level.On the 7th d the serum Cr and BUN levels in transplantation group were lower than those in control group.On the 28th d the levels of serum Cr(90.1?11.1) ?mol/L and BUN(8.0?1.5) mmol/L in transplantation group were significantly lower than those in control group(135.6?32.5) ?mol/L and(10.9?2.5) mmol/L,respectively(P

Objective To investigate the clinical effectiveness and safety of acupuncture at Zusanli (ST36),Taichong(LR3) and Hegu(LI4) points combined with three-step analgesic ladder for the treatment of gastric cancer pain.Methods Sixty patients with moderate and severe pain induced by gastric cancer were randomly divided into study group and control group,30 cases in each group.The study group was treated with acupuncture at Zusanli,Taichong and Hegu points combined with three-step analgesic ladder for gastric cancer pain management,and the control group was treated with three-step analgesic ladder only.After treatment for 7 days,the clinical effectiveness was evaluated with the average pain scores of Numerical Rating Scale(NRS),Quality of Life Scale(QOL-BREF by WHO),Self-rating Depression Scale(SDS).And the incidence of adverse reaction was also observed.Results (1) After treatment,the markedly-effective analgesic rate of the study group was higher than that of the control group (P ＜ 0.05).(2) After treatment,QOL scores of both groups were much improved as compared with those before treatment(P ＜ 0.05),and the improvement of the study group was superior to that of the control group(P ＜0.05).(3) The depression scores of both groups were much improved after treatment (P ＜ 0.05 compared with those before treatment),but the difference between the two groups was insignificant(P ＞ 0.05).(4) The incidence of adverse reaction of the study group was lower than that of the control group(P ＜ 0.05).Conclusion The effect of acupuncture at Zusanli,Taichong and Hegu points combined with three-step analgesic ladder is better than three step analgesic ladder alone for the treatment of gastric cancer pain.

Bacterium is dominant microflora population in human oral cavity, and the novel species and novel genus were discovered and named one after another. This article reviewed the major biological characteristics of 5 novel genus and 16 novel species isolated from the human oral cavity from 2009 to 2012.
Bacteria ; classification ; isolation & purification ; Humans ; Mouth

Objective To explore the effects of empowerment education model on glycemic control and self-management behavior of community patients with diabetes. Method A total of 120 diabetics from 2 different communities were set as the observation group and control group. The control group was treated by routine medications and health education and the observation group was intervened with empowerment education model. After intervention for 6 months, the patients′blood glucose index and the diabetes self-management behavior were assessed and compared by scale of the diabetes self-care activities (SDSCA) between the two groups. Result By the end of the study, the glycemic index and SDSCA score in the former group were both significantly decreased than those in the control group (P<0.05), indicating the patients in the experiment group had better glycemic control and self-management behaviors compared with those in the control group. Conclusions Empowerment education model can arouse the internal motivation of patients to change their behaviors from passive to active acceptance in the health education. Empowerment education model can enhance diabetes patients′self-management conduct and the glycemic level.

Objective: To study relationship between exercise and risk factors for cardiovascular diseases. Methods: A total of 750 military cadres retired or on the job, who received convalescence therapy in Guangzhou sanatorium, were enrolled as survey subjects, and divided into exercise group（488 cases）, and no- exercise group （262 cases）. The cardiovascular risk factors such as blood pressure, body mass index (BMI), fasting blood glucose（FBS）, total cholesterol （TC）, triglyceride （TG）and uric acid were measured. Questionnaires were used to survey mode of exercise, exercise frequency, exercise duration each time and total exercise time in a day. Results: 1. In no-exercise group, levels of systolic blood pressure, diastolic blood pressure, BMI, FBS, TC and TG were significantly higher than those of exercise group (P<0.05 all), but there were no significant difference in age and levels of HDL-C, LDL-C and uric acid between the two groups（P＞0.05 all）; 2. （1） Abnormal rate of risk factors for cardiovascular diseases （call for short was risk rate, similarly hereinafter）in different exercise duration each time （≥30 min、＜30 min）and exercise mode（walking, taijiquan）were no significant（P＞0.05 all）;（2）Risk rate in subjects who took exercise >60min in a day was significantly lower than that of those with ≤60min (29.41% vs. 45.59%), in subjects whose exercise intensity with RPE（Borg rating of perceived exertion scale）11~15 was significantly lower than that of with RPE <11 (33.67% vs. 44.64%), P<0.05 all;（3）Risk rate in subjects whose exercise frequency ≥3 times/week was significantly lower than that of with ＜3 times/week （31.47% vs.8.43%）, in subjects whose persistence time of exercise was 10～19 years was significantly lower than that of with ＜10 years, P<0.05 all. Conclusion: 1. Risk extent of cardiovascular diseases is lower in exercise group, and there is additive effect in risk factor for cardiovascular diseases; 2. take exercise >60min/d, Borg rating of perceived exertion scale 11~15, exercise frequency ≥3 times/week and long time persistence are conduce to decrease risk of cardiovascular diseases in middle-aged and aged persons.

Objective: To investigate the immunological mechanism of influenza A virus for murine S180 ascites sarcoma. Methods: After inoculation with S180 sarcoma cells, mice were i. p. injected with influenza A virus or vehicle 15 days. The average living time and survival rate of the mice were examined. The levels of IL-2, IL-6 and TNF-? were detected. The sarcoma cell's apoptosis was detected by DNA ladder, flow cytometry (FMC) , fluorescent microscope and e-lectron microscope (EM). Results: The average living time and survival rate of the mice injected with Influenza A virus were significantly longer or higher than that of the controls. The levels of IL-2, IL-6 and TNF-? also had the same differences. The apoptosis cells were detected by EM and fluorescent microscope. Sub-diploid peaks were observed by FCM a-nalysis and DNA ladder was seen after electrophoresis in the ascites cells. Conclusion: Our results demonstrated that the feasibility and potential of delivery of influenza A virus as a general means for the treatment of S180 ascites sarcoma.

OBJECTIVETo examine the flora samples from the tongue dorsum of the atrophic glossitis group and to discuss the relationship so as to provide a thinking pattern for therapy and a clue for deeper research.

METHODSTo collect personal information on 60 cases of atrophic glossitis and 40 cases of volunteers as control. The main items include general status, oral examination, salivary flow rate, pH value and bacteria test. All data were analyzed statistically.

RESULTS1) Among the 60 cases, 75.00% were female patients. Glosso-pain, dry mouth and taste loss were the most common symptoms. 2) In regard to the pathogenic factors, the systematic diseases were often visible, i.e. gastritis, coronary disease and anemia. 3) Oral hygiene of the patients was worse than that of the control group, the saliva flow rate and pH value were lower than that of the control. 4)The statistic analysis showed that the quantity of some bacteria of tongue dorsum and their detectable rate were different between the glossitis group and the control one, between the patients with atrophic glossitis who also suffered from different systematic diseases and the control group, and between the complete type and the partial type. These bacteria included Streptococcus sanguis, Stomatococcus, Staphylococcus aureus, Saccharomyces, etc.

CONCLUSIONAtrophic glossitis is the consequence co-affected by host, circumstances of oral cavity and bacteria. The tangible relationship between atrophic glossitis and micro-ecological changes on glossal dorsum has not been confirmed yet, however, flora change on dorsum may have relations with occurrence, and development of the disease.

Objective To understand the clinical features of patients with human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) complicated by cytomegalovirus (CMV)viremia.Methods The clinical data of 249 cases of HIV/AIDS patients hospitalized in Beijing Ditan Hospital from Oct 2008 to Nov 2009 were analyzed retrospectively,in which 43 HIV/AIDS patients were diagnosed with CMV viremia.The symptoms and signs,cerebrospinal fluid (CSF)tests,and pathological detections by bronchoscope,gastroscope and fibercoloscope were collected.The database was set up using Excel software.The association between cellular immunity and CMV DNA level was determined by SPSS12.0 software.Results Forty-three patients (17.3%)were diagnosed with CMV viremia by positive results of CMV pp65 antigen and CMV DNA tests; 14 patients manifested retinal bleeding or infiltration and 4 patients displayed retinal fibrosis; 1 patient was diagnosed with CMV pneumonitis by pathological results of bronchoalveolar lavage fluid.Low level of CD4+ T lymphocytes and CMV DNA levels were positively correlated.Conclusions CMV pp65 antigen and CMV DNA should be detected in HIV/AIDS patients with CD4+ T lymphocytes less than 100 × 106/L and anti-CMV treatment should be given according to the results.Ophthalmologic examination and bronchoalveolar lavage fluid pathological detection are effective methods in diagnose of CMV retinitis and pneumonitis.