Lapareseopic colectomy is commonly performed,but laparoscopic extended right hemicolectomy with D3 lymphadenectomy for cancer located at hepatic flexure of the colon is a complex procedure,even in the hands of experts.Laparoscopic dissection of the lymph nodes around the middle colonic and right gastroepiploic vessels are so complicated that precise vascular anatomy and surgical plane are essential to complete this procedure safely.We herein describe a standard technique for performing laparoscopic extended right hemicolectomy with D3 lymphadenectomy using a medial-to-lateral approach.The main surgical plane of this procedure is the right Toldt's space.The superior mesenteric vein (SMV) is the most important anatomical landmark of vascular dissection.Medial-to-lateral approach makes it easy to locate the right Toldt's space and the SMV.

Objective To investigate the feasibility and safety of laparoscopic myomectomy in patients with large uterine myoma and the tumor located at special sites. Methods A retrospective study of 142 patients underwent laparoscopic myomectomy was done.In the patients,all the myomas were ≥10 cm in diameter;located at the cervix,uterus posterior wall near to the isthmic uteri,or broad ligament.The procedure was completed according to the volume and location of the tumors.For large myomas,the muscle layer was resected to remove the tumor,after removing the major part of the lesion(cut into pieces before moving out),the rest small parts were cut completely.Ligation was used for large tumors with sufficient blood supply in the surface or subserous myomas.For the myomas located in the broad ligament,the ligament was opened to pull out the tumor,and then the tumor was cut into pieces and resected at the root. Results The procedure was completed by laparoscopy in all of the patients,except in one who received laparoscopy-assisted resection.The removed tumors weighed 44 to 1903 g.The operation time ranged from 30 to 175 minutes.Intraoperative blood loss was 10 to 800 ml.No serious complications occurred.Among the cases,follow-up was available in 68 patients for 2 years.During the period,ultrasonography showed recurrent myoma in 2 patients. Conclusions With the improvement of the skills of laparoscopic myomectomy,indications for the laparoscopic operation can be widened.

Aim To investigate the effects of Cariporide on expression of intercellular adhesion molecule-1(ICAM-1) and P-selectin and adhesion of monocytes and endothelial cells induced by lysophophatidylcholine(LPC).Methods The ratio of adhesion of monocytes and endothelial cells was assessed by measuring protein content.The expression of ICAM-1 and P-selectin in endothelial cells was examined by enzyme-linked immunosorbent assay(ELISA).Intracellular pH of endothelial cells was measured with 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein(BCECF).Results The results showed that LPC enhanced the adhesion of monocytes and endothelial cells in a concentration-dependant and time-related manner.The dosage of 5,10 and 20 ?mol?L-1 Cariporide reduced the adhesive ratio of endothelial cells induced by LPC(5 mg?L-1)from 36% to 23%,18% and 16%(P

Objective: To assess the tissue reactions of dogs to a new nanohydroxyapatite. Methods:Three dogs were used for root canal filling experiment, mandibular implanting and subcutaneous implanting experiment. AH-plus root canal sealer was used as control. The dogs were killed after implantation for 2,4 and 12 weeks separately. The tissue reaction was assessed by X-ray and light microscope examination. Results:①Root canal filling experiment: no inflammatory reaction was detected in specimens of n-HA group. In AH-plus group, implantation caused a middling inflammatory reaction after 2 weeks and 4 weeks. After 12 weeks the reaction of apical tissues was slight. ②Mandibular implanting experiment: no inflammatory reaction was showed by all n-HA groups and the group of AH-plus implanted for 12 weeks.Mild inflammatory reaction was observed in the groups of AH-plus implanted for 2 weeks and 4 weeks.③ Subcutaneous implanting experiment:after AH-plus was implanted for 2 weeks and 4 weeks, middling inflammatory reaction was observed. The group of n-HA implanted for 2 weeks showed the same reaction. No obvious inflammatory reaction was found in the other groups. Conclusion:n-HA shows better tissue compatibility than AH-plus.

Objective To study the pharmacological effects of Qinggong Decoction on sedation and hypnosis. Methods Fifty mice were randomly divided into five groups: NS group, diazepam group, and low-, mid-, high-dose Qinggong Decoction group. Diazepam group was given 0.1 mL/10 g diazepam, and other four groups were given 0.2 mL/10 g corresponding drugs. Spontaneous activities before and after ig. were recorded and the sleeping time of mice with pentobarbital sodium (superthreshold dosage) and the number of the sleeping mice with pentobarbital sodium (subthreshold dosage) were measured. Convulsion model induced by nikethamide was used to observe the change of the seizure rate in mice after given Qinggong Decoction. Results Qinggong Decoction (3.0, 6.0 g/kg) could obviously inhibit spontaneous activities of mice, prolong the sleeping time of mice with pentobarbital sodium (superthreshold dosage) and increase in the number of the sleeping mice with pentobarbital sodium (subthreshold dosage), and had markedly anticonvulsant effect in mice induced by nikethamide. Conclusion Qinggong Decoction has obvious effects of sedation, hypnosis and anticonvulsion.

OBJECTIVE To investigate the hand hygiene situation of medical staff in order to carry out supervising and monitoring on their hand hygiene pertinently.METHODS The hand hygiene monitoring data from Jan 2004 to Dec 2007 were retrospectively analyzed.RESULTS The total qualified rate of medical staff hand hygiene was 91.4%;the qualified rate of hand hygiene monitoring for doctors was 89.8%,and it was 96.7% for nurses;the qualified rate of medical staff working in the type-Ⅱenvironment was 93.6%,and the qualified rate of those working in the type-Ⅲ environment was 95.2%;for medical students and refresher doctors,it was 81.0 %.CONCLUSIONS The medical staff still lack the knowledge on hands' cleanliness and disinfection.The supervisors should strengthen monitoring on hand hygiene;some new regulations should be established and practiced,and the hand washing equipments should be updated;the medical staff's compliance of hand hygiene should be improved,and their conceptions of hand hygiene should be strengthened;all-sided originalities on hand hygiene should be carried out in order to reduce the occurring of hospital infections.

Objective To study the feasibility of tubal reversal after sterilization by laparoscopy.Methods From May 2002 to December 2005,31 patients with tubal ligation received laparoscopic tubal reversal by one-core or two-dot apposition and demixing suture of the serosa.Results The laparoscopic tubal reversal was successful in all the 31 cases.Thirty of the patients were followed up for 8-24 months(mean,16 months);27 of them had intrauterine gestation,3 were non-pregnant;none of them had ectopic gestation.Conclusions Laparoscopic tubal reversal after sterilization can satisfy with the desire and principle of microsurgery.It is feasible for clinical application and has good outcomes.

0.05).Conclusion these results suggest that ?_2-AR 16、27 locus genetic polymorphism is not correlated with asthma.

Early diagnosis and symptomatic therapy of amyotrophic lateral sclerosis(ALS)can profoundly influence care and quality of life of the patient and relatives,and may increase survival time.Medication with riluzole should be initiated as early as possible.PEG is associated with improved nutrition and should be inserted early.Noninvasive positive pressure ventilation improves survival and quality of life.Palliative end-of-life care should be provided to ALS patient.

0.05),while the activities of SOD of bone tissue were lower(P