Clinicopathological parameters are important to predict the prognosis of esophageal squamous cell carcinoma (ESCC),they mainly include TNM stage related indexes of the tumor,tumor length,vessel and nerve invasion, tumor budding, peripheral blood cells, etc. To predict the prognosis of ESCC patients accurately is the prerequisite of precise treatment and the key to improve the patients survival rate and survival quality.

Gastric cancer has high heterogeneity,and the traditional histopathologic classification has a limited significance for clinical work.Along with the dramatic development of molecular detection technology,it may be much more helpful for the treatment and prognosis to use the molecular classifications.Recently,there have been many researches on the molecular classification of gastric cancer,such as Tan type,Lei type,clonality type,The Cancer Genome Atlas (TCGA) type,Asian Cancer Research Group (ACRG) type,and so on.All of these molecular classification methods have respective advantages and disadvantages.

Objective To compare the effects of two different parenteral nutrition solutions on nutritional status of premature infants,such as the early physical,serum prealbumin,retinol binding protein and albumin.Methods We collected 61 cases of premature infants in neonatal intensive care unit of the Third Military Medical University Xinqiao Hospital,which were divided into 2 groups of traditional parenteral nutrition (TTPN) and early parenteral nutrition (ETPN).Group TTPN geted 6％ paediatric compound amino acid 0.5 g/(kg·d) at24 hours after birth,daily increasing 0.25 ～0.5 g/(kg·d),up to 3.0 g/(kg·d).The 20％ long-chain fat emulsion 0.5 g/(kg ·d) was applied at 3 days after birth,daily increasing 0.25 ～ 0.5 g/(kg·d),up to 3.0 g/(kg·d).Group ETPN get 6％ paediatric compound amino acid 1 g/(kg·d) at 12 ～24 hours after birth,daily increasing 0.5 g/(kg·d),up to 3.0 ～ 0.5 g/(kg· d).The 20％ long-chain fat emulsion 0.5 ～ 1.0 g/(kg·d) was applied after 24 hours after birth,daily increasing 0.5 g/(kg· d),up to 3.0 g/(kg· d).We calculate calorie and amino acid intake of the two groups,and compared the weight,length,head circumference growth,serum prealbumin,retinol-binding protein and albumin levels of the two groups.Results The amino acid and total caloric intake of Group ETPN were significantly higher than those of Group TTPN (P ＜ 0.05).No statistical differences in the albumin,prealbumin,and retinol binding protein level of the two groups at birth (P ＞ 0.05).However,the prealbumin and retinol-binding protein level of group ETPN significantly higher than those of Group TTPN (P ＜ 0.05) at 7 days after birth.Conclusion Early parenteral nutrition in early life can effectively improve physical growth and nutrient metabolism status of premature infants.Prealbumin and retinol-binding protein are sensitive to evaluate the protein nutritional status in premature infants.

Objective To investigate the effects of early hemofiltration combined with traditional Chinese medicine on nasal feeding on severe acute pancreatitis (SAP) patients. Methods Seventy four patients were divided into traditional Chinese medicine group (group A, 35 cases) and traditional Chinese medicine+hemofiltration group (group B, 39 cases). In group A, patients were given a serious of procedure including fasting, gastrointestinal decompression, fluid resuscitation, inhi-bition of pancreatic secretion, antibiotic prophylaxis, parenteral nutrition and traditional Chinese medicine on nasal feeding;In group B, patients received continuous veno-venous hemofiltration treatment(also called Continuous Renal Replacement Therapy,CRRT) in addition to the procedures receiving by group A. On admission and the first, 3rd, 7th days post-treatment, the scores of acute physiology and chronic health evaluation (APACHEⅡ), tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8) levels were recorded. Length of hospital stay, local and systematic complications, surgical inter-vention, mortality and hospitalization expenses were compared between two groups. Results On admission, no statistical significance was seen in the hematocrit, white blood cell count, lactic acid dehydrogenase, blood urea nitrogen, blood glu-cose, APACHEⅡscore, Ranson’s score and classification of etiology between these two groups (P>0.05). But APACHEⅡ, TNF-α, IL-6, IL-8 were decreased significantly in group B than in group A, after the first, 3rd, 7th days post-treatment (P＜0.05). Compared with group A, the length of hospital stay, local complications, systemic complications, surgical interven-tion, mortality and hospitalization expense were lower in group B. Conclusion Traditional Chinese medicine on nasal feed-ing combined with early hemofiltration could effectively reduce complications, incidence of organ dysfunction and could im-prove the prognosis of SAP patients.

Objective To analyze the epidemiological characteristics of measles in the floating population in Daxing District from 2002 to 2015 and to provide a specific guidance for the control and preven-tion of measles. Methods The epidemiological characteristics of measles in the floating population in Dax-ing District were analyzed based on the data collected from Diseases Reporting Information System and Ques-tionnaires for measles cases. Results A total of 1 263 measles cases were reported in the floating population in Daxing District from 2002 to 2015, accounting for 73. 09% of all reported cases in that area. Among the 1 263 measles cases, 1 027 cases (81. 31%) were reported from March to June. The top 5 towns with high incidence of measles were Huangcun, Jiugong, Xihongmen, Yizhuang and Yinghai, from which 80. 29% of measles cases were reported. Age distribution of the measles cases showed that 145 cases were children younger than 8 months, 412 cases were children aged 8 months to 14 years old and the rest 706 cases were a-bove 14 years old. And of all cases, only 224 cases had measles vaccine immunization history. Conclusion It is necessary to maintain the high vaccination rate of 3 doses of measles vaccine by multi-approaches and increase the immunization rate in migrant workers. Moreover, strengthen the surveillance for measles is also an effective way for measles control and elimination.

Objective To observe the effects of different levels of sodium arsenite ( NaAsO2) on mRNA expression of pigment epithelium-derived factor (PEDF) and apoptosis-related factors in PC12 cells ( rat neuron properties pheochromocytoma). Methods PC12 cells were treated with different levels of NaAsO2 [0 (control group), 2, 5, 10 μmol/L] for 24 hours. The mRNA expression of PEDF and apoptosis-related factors (Bax, Bcl-2) were detected by real-time quantitative PCR. Results There were significant differences in the mRNA expressions of PEDF between the 4 groups (F=102.28, P<0.05), the mRNA expressions of PEDF in the group of 2, 5, 10μmol/L (0.70 ± 0.07, 0.33 ± 0.04, 0.23 ± 0.10) was lower than that of control group (1.15 ± 0.11, P< 0.05); there were no significant differences in the mRNA expressions of Bax between the 4 groups (0, 2, 5, 10 μmol/L groups: 0.95 ± 0.12, 0.80 ± 0.11, 0.88 ± 0.11, 1.01 ± 0.11, F= 2.01, P> 0.05); there were significant differences in the mRNA expressions of Bcl-2 between the 4 groups (F=19.87, P<0.05), the mRNA expressions of Bcl-2 in the group of 2, 5, 10 μmol/L (0.65 ± 0.03, 0.49 ± 0.04, 0.57 ± 0.09) were lower than that of control group (0.95 ± 0.11, all P<0.05);there were significant differences in the mRNA expressions of Bax/Bcl-2 between the 4 groups (F=8.352, P<0.05), the mRNA expressions of in the group of 5, 10μmol/L (1.80 ± 0.72, 1.82 ± 0.36) were higher than that of control group (1.02 ± 0.24, all P<0.05). Conclusion NaAsO2 may increase the expression of apoptosis-related factorsBax/Bcl-2 mRNA by decreasing the expression of PEDF mRNA in PC12 cells, leading to apoptosis in PC12 cells.

Objective:To evaluate the value of serum pepsinogen Ⅰ and Ⅱ combined with gastrin-17 in screening precancerous lesions of gastric cancer in physical examination population.Methods:Serum pepsinogen, gastrin-17 and Helicobacter pylori (Hp) antibody were detected in 18 354 physical examination people from July to December 2017 in Wenrong Hospital, Hengdian, Dongyang. The patients were divided into youth group (18 to 39 years old), middle-aged group (40 to 59 years old) and elderly group (≥60 years old) according to their ages. The correlation between the serological level of the above indexes and age was analyzed; according to the new ABC method, the test results were divided into groups A, B, C and D. The patients in group C and D were examined by gastroscopy. The differences of gastric mucosal atrophy or intestinal metaplasia and other precancerous lesions detected by gastroscopy in different age groups were compared.Results:Finally, 18 354 cases were enrolled, including 9 614 males and 8 740 females. With the increase of age, the proportion of group C and D increased gradually. In group C, 181 cases underwent gastroscopy, including 39 cases of atrophic gastritis, 29 cases of intestinal metaplasia and 3 cases of dysplasia/intraepithelial neoplasia, the detection rate of precancerous lesions was 39.23%; in group D, 94 cases underwent gastroscopy, including 22 cases of atrophic gastritis and 13 cases of intestinal metaplasia, the detection rate of precancerous lesions was 37.23%. The proportion of gastric precancerous lesions in group C and D was 29.63% in the young group, 69.70% in the middle-aged group and 71.58% in the old group, respectively. There was significant difference compared with the young group ( P<0.01); atypical hyperplasia occurred in 2.02% and 9.47% of the middle-aged group and the elderly group. Conclusions:The combined detection of serum pepsinogen Ⅰ and Ⅱ and gastrin-17 levels is of great value in the screening of precancerous lesions of gastric cancer; when this method used for early gastric cancer screening in healthy population, it is necessary to consider the influence of age for the risk stratification of gastric cancer.

Objective To study the difference of microRNA ( miRNA ) expression between two groups of early stage ( pT1N0 ) esophageal squamous cell carcinoma ( ESCC ) patients who had different outcome and the prognostic significance of different miRNA in metastatic of early ESCC , and to identify useful prognostic markers in the selection of appropriate treatment for early ESCC patients.Methods TaqMan human miRNA arrays and bioinformatics were used to detect and analyze the expression profiles of miRNAs in the two groups , and RT-PCR was used to verify the differences in miRNA expression.Results The miRNA arrays revealed a total of 41 markedly changed miRNAs in the survival group compared with the death group.Bioinformatics analysis , prediction and significant function analyses of targeted genes and pathway analysis identified that miR-27a, miR-143 and miR-886-5p levels were increased or decreased by seven-folds or more.The enriched target genes were GRB2, SOS1, MAPK1, EGFR, CBL, SPRY2, RPS6KA5, IGF1R, NGFR, MAPK14 and CREB1.These genes were significantly related to the following signaling pathways , i.e.Sprouty regulation of tyrosine kinase signals pathway , Erk1/Erk2 Mapk signaling pathway and transcription factor CREB and its extracellular signals.Conclusions miR-27a, miR-886-5p, and miR-143 may be potential prognostic markers of metastasis for early ESCC.The detection of these miRNAs plays a directive role for the treatment options of early ESCC.The regulation of targeted genes and mechanism remain to be further studied.

Objective? To explore the effects of WeChat combined with motivational interviewing in transitional care among young and middle-aged hypertension patients. Methods? By convenient sampling, we selected 129 young and middle-aged hypertension inpatients who were hospitalized in a classⅢ grade A hospital from June 2017 to March 2018 as subjects. All of the patients were randomly divided into observation group (n=66) and control group (n=63). In the control group we adopted routine health education and routine telephone follow-up after discharge. Apart from that, for the observation group we carried out transitional care by WeChat combined with motivational interviewing. The medication adherence was compared with the Morisky medication adherence scale, and the blood pressure control rate was observed and compared between the two groups. Results? Three months after intervention, the medication adherence of two groups all improved compared with that before intervention;the score of medication adherence of observation group and control group was (4.7±1.7) and (3.8±1.5) respectively with a statistical difference (t=3.192, P＜ 0.05). After intervention, the blood pressure control rate of observation group and control group was 60.3% and 51.5% respectively with a statistical difference (χ2=5.645, P＜ 0.05). Conclusions? Transitional care by WeChat combined with motivational interviewing can improve medication adherence and blood pressure control of young and middle-aged hypertension patients which is worthy of clinical application.

OBJECTIVETo study the difference of microRNA (miRNA) expression between two groups of early stage (pT1N0) esophageal squamous cell carcinoma (ESCC) patients who had different outcome and the prognostic significance of different miRNA in metastatic of early ESCC, and to identify useful prognostic markers in the selection of appropriate treatment for early ESCC patients.

METHODSTaqMan human miRNA arrays and bioinformatics were used to detect and analyze the expression profiles of miRNAs in the two groups, and RT-PCR was used to verify the differences in miRNA expression.

RESULTSThe miRNA arrays revealed a total of 41 markedly changed miRNAs in the survival group compared with the death group. Bioinformatics analysis, prediction and significant function analyses of targeted genes and pathway analysis identified that miR-27a, miR-143 and miR-886-5p levels were increased or decreased by seven-folds or more. The enriched target genes were GRB2, SOS1, MAPK1, EGFR, CBL, SPRY2, RPS6KA5, IGF1R, NGFR, MAPK14 and CREB1. These genes were significantly related to the following signaling pathways, i.e.Sprouty regulation of tyrosine kinase signals pathway, Erk1/Erk2 Mapk signaling pathway and transcription factor CREB and its extracellular signals.

CONCLUSIONSmiR-27a, miR-886-5p, and miR-143 may be potential prognostic markers of metastasis for early ESCC. The detection of these miRNAs plays a directive role for the treatment options of early ESCC. The regulation of targeted genes and mechanism remain to be further studied.

Biomarkers, Tumor ; genetics ; metabolism ; Carcinoma, Squamous Cell ; genetics ; pathology ; surgery ; Esophageal Neoplasms ; genetics ; pathology ; surgery ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Lymphatic Metastasis ; MicroRNAs ; metabolism ; Neoplasm Metastasis ; Neoplasm Staging ; Signal Transduction