OBJECTIVE To discuss the nosocomial infection risk factors and preventive strategies in human assisted reproductive technology(ART)laboratory.METHODS The nosocomial infection risk factors in ART laboratory were analyzed,and the corresponding measures were set up.RESULTS In ART laboratory,there were nosocomial infection risk factors existing in management system,preventive process,condition of laboratory,technical program and garbage management.Corresponding measures were established to control the risk of nosocomial infection.CONCLUSIONS The nosocomial infection in ART laboratory can effectively be prevented through establishment of perfect control system,strict operation of the rules and improving technical programs.

Objective To observe the effects of Bushen Huoxue Decoction on clinical symptom, vertigo degree and cognitive function for elderly patients with chronic cerebral circulation insufficiency (CCCI); To explore its possible mechanism. Methods Totally 70 cases were randomly divided into observation group and control group according to the random digits table method, with 35 cases in each group. The two groups were given Western medicine consisting of blood pressure control, glucose control and blood lipid control. Based on this, the control group was given Flunarizine Hydrochloride capsules, 5 mg once a day orally before sleeping. The observation group was given Bushen Huoxue Decoction, one dose a day and taken twice a day. The treatment lasted for 30 days. The TCM symptom score, Chinese version of dizziness handicap inventory (DHI) and Montreal cognitive assessment (MoCA) score were observed. The mean blood flow velocity (Vm) of basilar artery (BA), bilateral vertebral artery (VA), bilateral middle cerebral artery (MCA) and the content of plasma acid phospholipid (AP) were detected, and the clinical efficacy was evaluated. Results The total effective rate of the observation group was 94.3％ (33/35), which was significantly higher than that of 82.9％ (29/35) in the control group, with statistical significance (P<0.05); after treatment, the TCM symptom score, DHI score, and the content of plasma AP were significantly lower than those of pre-treatment in the same group (P<0.05); the MoCA score and the Vm of VA, LBA, RBA, LMCA, RMCV were significantly higher than those of pre-treatment in the same group (P<0.05); the TCM symptom score, DHI score, MoCA score, the Vm of LVA, LMCA, RMCA and the content of plasma AP in the observation group were improved better than those in the control group, with statistical significance (P<0.05). Conclusion Bushen Huoxue Decoction has an obvious therapeutic effects for elderly patients with CCCI, which can improve clinical symptom, vertigo degree and cognitive level, which possible mechanism is related to increasing the cerebral blood flow velocity and reducing the content of plasma AP.

Objective To explore the clinical characteristics of Chronic obstructive pulmonary diseases(COPD)(≥65 years old,acute episode)with active pulmonary tuberculosis.Methods62 of COPD(≥65 years old,acute episode)with active pulmonary tuberculosis from 2002 to 2007 in hospital were reviewed and analyzed.ResultsThe attack rate of active pulmonary tuberculosis is 7.3% in COPD(≥65 years old,acute episode).By Clinical analysis showed clinical manifestation and chest X-ray or CT were not typical,but positive morbility of acid-fast bacilli sputum smear and bronchoalveolar lavage fluid was higher.ConclusionThe result suggests that COPD(≥65 years old,acute episode)patients are high-risk group of active pulmonary tuberculosis and need to be diagnosed early as soon as possible.

The recent studies have shown a significant relationship between persistent organichorine pollutants(POCPs) and semen quality. The biological characters and source of POCPs, the effect mechanisms of POCPs on semen quality, sperm structure and function were reviewed in this paper. The epidemiological evidences for the reproductive disturbance of men caused by POCPs were discussed and the research direction in the future was pointed out.

Objective To investigate the changes of plasma free carnitine (FC) concentrations in preterm infants supplemented with L-carnitine, and to provide a reference for routine preterm infants L-carnitine supplements. Methods A total of 99 preterm infants supplemented with 10 mg/(kg·d) L-carnitine on days 2 and 5 after birth, and 65 full term infants from Department of Neonatology, the Fifth People′s Hospital of Dongguan during July 2014 to December 2015 were recruited in this study , and filter-paper blood spots were collected by heel prick on days 1, 3 and 7. FC was measured using electron spray ionization (ESI) tandem mass spectrometry (MS-MS). Results Concentrations of FC decreased steadily from day 1 to day 7 in full term infants , while it remained the same level during the first week after birth as at birth. Additionally, concentrations of FC were significantly higher in preterm infants than full term infants on day 1 after birth. Conclusions The reasonable L-carnitine supplements may keep the levels of plasma FC at the levels at birth , which is important for fatty acid metabolism in preterm infants.

Objective To evaluate accuracy and consistency of the coagulation convention that involves PT, INR, FIB, APTT on two coagulation analysators(ACL-9000 and ACL-200, Beckman-Coulter Corporation, US). Methods The repeatability test was used to assess the precision, the accuracy and consistency of the coagulation convention on two coagulation analysators were evaluated by comparison and analysis. Results The repeatability of related test results based on ACL-9000 coagulation analysator was better that CV value less than 5%, but the repeatability of abnormal specimens FIB based on ACL-200 was poor that CV value of 6.6%. Through comparing ACL-9000 analyzer, the test results of ACL- 200 coagulation analysator has good correlation with it, correlation coefficient ?﹥0.975. Conclusion In the condition of the excellent precision and stability, we can also find system errors existed in the analyzer on time by verificating. Through being adjusted and calibrated, the examination department can deliver the results of accuracy consistency.

To investigate the change of plasma D-d imer in patients with chronic pulmonary heart disease． The plasma was measured in 36 patients with chronic pulmonary heart disease and in 25 controls ．Results：The D-dimer level as significantly higher in the before treatment patients than in the after treatment patients (P<0.0l )． Conc luslons: D-dimer can be used in diagnosis of ．Thrombi in small pulmonary arteri e s andarterioles．

Objective To investigate the influence of host animals on epidemics of hemorrhagic fever with renal syndrome (HFRS) so as to provide a basis for effective control of HFRS.Methods From the national infectious disease network direct reporting system,the incidence of HFRS cases diagnosed by direct diagnosis of medical institutions in Qingdao was collected from 2011-2015.We captured rats indoor and outdoor by night trapping method quarterly and calculated the capture rates from 2011-2015 in Qingdao areas.The incidence of HFRS in different regions and the change of seasonal growth,the distribution of host animals,the characteristics and distribution of animals,and the seasonal variation of dominant species were analyzed and a database was set up and statistic analysis was conducted by SPSS 13.0.Results The peak incidence rate of HFRS in Qingdao areas occurred in 2012 (3.54/100 000) and presented a decrease trend year by year (x2 =64.15,P ＜ 0.05),but there were different characteristics among the epidemic areas,and lowest in 2015 (1.68/100 000).And the peak presented a two-peak pattern which was mainly an autumn peak and a gentle peak in late spring and early summer.The epidemics were gradually decreased from the rural areas to the urban fringes and then the urban areas.The seasonal variation was disappeared gradually.There was a heavy epidemic intensity in areas with a high capture rate and a complex type of host animals.The epidemic peak was in consistence with the distribution of rats.Capture rates were different among the epidemic areas.The capture rate in Jiaonan was the highest [5.32％(2 886/54 287)] and lowest in Pingdu [1.77％ (258/14 584)].The mean (x2 =820.39,P ＜ 0.05) and annual capture rates (x22011-2015 =32.61,356.24,233.07,129.33,33.42,all P ＜ 0.05) among epidemic areas were different.In the third quarter the accumulated capture rate was the highest [4.69％ (1 187/25 301)].In total 8 kinds of host animals were captured and the dominant species were brown rat [30.27％ (1 235/4 080)],house mouse[29.75％ (1 214/4 080)] and striped field mouse [16.25％ (663/4 080)].Conclusions The epidemic intensity of HFRS is related to the densities and the types of host animals.The gradually decreased epidemic pattern from the rural areas to the urban fringes and then the urban areas may be related to urbanization and improved health behaviors.

High temperature and humidity stress during seed growth and development of spring soybean can result in seed deterioration in South China. We isolated two genes (GmSBP and GmSBPL) encoding putative SBP proteins from soybean (Glycine max (L.) Merr.) to study their biological functions and response to abiotic stress,. The two SBP proteins are hydrophilic and incomplete membrane ones. Real-time quantitative (RT-PCR) analysis reveals that the expression of the two genes in the developing seeds of the seed deterioration resistant cultivar Xiangdou No. 3 and sensitive cultivar Ningzhen No. 1 was significantly affected by high temperature and humidity treatment. Meanwhile, the levels of sucrose and soluble sugar in the developing seeds of both cultivars were also affected under high temperature and humidity stress. During seed growth and development, the expression of the two genes as well as the levels of sucrose and soluble sugar reached the highest at 30 days after flower. GmSBP2 and GmSBPL were found to be differentially expressed in different soybean tissues. Sub-cellular localization indicated that two genes were located in cytoplasm and cell membrane. Our results indicate that GmSBP2 and GmSBPL might be involved in the response to abiotic stress, which will enrich our understanding of pre-harvest seed deterioration and resistance in soybean from one side.
China ; Gene Expression Regulation, Plant ; Genes, Plant ; Membrane Transport Proteins ; genetics ; Plant Lectins ; genetics ; Real-Time Polymerase Chain Reaction ; Seeds ; Soybean Proteins ; genetics ; Soybeans ; genetics ; Stress, Physiological

Objective To investigate the association between chromosome variations,abnormalities and male reproductive hor-mones level with spermatogenesis.Methods The chromosome karyotype,serum reproductive hormone including FSH,LH,T,PRL and E2,and semen were detected in 147 patients with male infertility or recurrent sponotaneous abortion.The results were per-formed the comparative analysis.Results Serum FSH,LH level and the incidence rate of azoospermia in the chromosome abnormal-ity group were significantly higher than those in the chromosome variation group and the normal group(P <0.05,P <0.01),serum T level was significantly lower than that in the chromosome variation group and the normal group(P <0.05).Serum FSH and the incidence rate of oligospermia in the Y chromosome variation group were significantly higher than those in the autosomal variation group(P <0.05).The incidence rate of azoospermia had no statistically significant difference between the Y chromosome variation and the autosomal variation group(P >0.05).Serum FSH,LH level and the incidence rate of azoospermia in the sex chromosome abnormality group were obviously higher than those in the autosomal abnormality group(P <0.05),the serum T level was signifi-cantly lower than that in the autosomal abnormality group(P <0.05).Conclusion The chromosome variation and abnormality are closely related with the reproductive hormones disorder and spermatogenetic function disorder.The obvious increase of serum FSH, LH level and obvious decrease of T level caused by sex chromosome variation and abnormality is one of the pathogenesis of oligo-spermia and azoospermia.