Leptin is type I cytokine detected recently.It takes part in physiological action such as energy balance, fat storage and process of metabolism,reproduction and hemopoiesis in vivo.Leptin may be an important role between adipose tissue and genital system.Lack or excessive of leptin is related to infertility in some diseases involving in nutritional status. Leptin takes part in female infertility pathogenesis in hypothalamus pituitary gland gonad axia and other respects.

The compounded prescription of Chinese crude drug is a key problem in the study of traditional Chinese medicine.A new research field that focuses on the group of effective fractions of Chinese crude drugs becomes more important as the result of combination of the theorem of traditional Chinese medicine and techniques of modern medicine.Up to date,the researches on this field are preliminary,remaining a lot of subjects to be explored.

OBJECTIVE:To assess the stability of the quality control of FPIA for monitoring of CsA and MEIA for monitoring of FK506.METHODS:The quality control on the blood samples of patients receiving therapeutic drug monitoring of CsA and FK506 from June to August in 2007 were subjected to a continual assessment and analysis to investigate the stability and accuracy of the quality control.RESULTS:The mean recoveries of the low,middle and high concentrations of CsA and FK506 were 93.14%～97.65% and 97.70%～103.09% respectively,with RSD at 4.41%～5.37% and 10.44%～14.23% respectively,which accorded with the requirement for biological sample determination stated in the Chinese Pharmacopoeia.CONCLUSION:The methods of FPIA for monitoring of CsA and MEIA for monitoring of FK506 were accurate,and worth to be applied in clinical to guide individualized medication.

OBJECTIVE:To investigate the characteristics of adverse drug reactions (ADR) caused by quinolones in our hospital and to promote their rational use in clinic. METHODS: By a retrospective review,a total of 199 quinolones-induced ADR cases collected from May 2004 to December 2007 in our hospital were analyzed statistically in respect of patients' gender and age,drug species involved,past ADR history,route of administration,clinic manifestations,etc. RESULTS: A total of 9 kinds of quinolones involved in the 199 ADR cases,of which,118 cases were induced by levofloxacin,16.1% had past ADR history,93.5% of the ADR cases were induced via intravenous drip,and most common presentations of the ADR were lesions of skin and its appendants as well as digestive system. There were 7 serious ADR cases. CONCLUSION: The indications of quinolones should be controlled strictly,which should be preferably administered orally and monitoring of its administration should be emphasized in clinical practice to decrease and avoid the occurrence of ADR.

OBJECTIVE:To establish the quality standard for Yiwei granule. METHODS:TLC was conducted for the qualitative identification of Coptis chinensis,Corydalis yanhusuo and Schisandra chinensis;HPLC was conduced for the content determination of paeoniflorin. The column was XDB C18 with mobile phase of methanol-water(25:75,V/V)at flow rate of 1.0 ml/min,detection wave-length was 230 nm and injection volume was 20μl. RESULTS:The TLC showed clear spots and good separation. The linear range of paeoniflorin was 7.575-60.6 μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2.0%,recov-ery was 96.97%-102.51%(RSD=1.92%,n=9). CONCLUSIONS:The method is simple and reproducible,and can be used for the quality control of Yiwei granule.

Purpose A systematic analysis of the articles published in the Chinese Journal of Medical Imaging from year 2013 to 2015 was performed to investigate its features of acceptance and publication for future decision making.Methods The printed journal and retrieved data from the Chinese Hospital Knowledge Database was analyzed using biometrical index,including the number of the papers,subspecialties,publication types,medical imaging modalities,MeSH frequency,citation and download.Results From year 2013 to 2015,there were 770 papers published,21.4 articles per issue and 0.267 articles per page in average.The top three subspecialty were abdominal imaging,chest imaging and reviews/comments,accounting for 17.27％,9.61％,9.48％,respectively.The top three modalities were CT,ultrasound and MRI,accounting for 39.61％,38.31％,29.87％,respectively.The original research papers were 584,accounting for 75.84％.The frequent MeSH subjects were differential diagnosis,angiography,pathology,computer-assisted image processing and contrast agents.Totally,there were 2395 citations and averagely 3.11 citation for each paper.The top citation was 36.There were 71317 downloads and 92.62 for each paper in average,the top download was 499.Conclusions The publications and its density per page increased annually from 2013 to 2015.The subspecialty were fully covered,the papers focus on original researches and clinical applications.The publication theme could follow the tendency of the imaging technology and had good and steady effects in the field.

Objective:To compare the steam distillation( SD) and supercritical CO2 ( SFE-CO2 ) method in the extraction of vola-tile constituents from such 3 traditional Chinese drugs as cnidium monnieri, radix sileris and atractylodes lancea in compound Kuhuang prescription. Methods:Essential oil was extracted from the 3 traditional Chinese drugs by SD and SFE-CO2 method, respectively. The components were identified by GC-MS, and their relative contents were calculated with peak area normalization method. Results:To-tally 36 components were isolated and identified using SD extraction and 31 components were identified using SFE-CO2 extraction, a-mong them, 22 components were the same and their relative molecular weight mainly concentrated within 200-230. Conclusion: SFE-CO2 method can extract effective components from the 3 traditional Chinese drugs in compound Kuhuang prescription with higher selec-tivity, which is the more suitable extraction method for essential oil from the prescription.

OBJECTIVE:To optimize the water-extraction technology of Compound kuhuang formulation. METHODS:The wa-ter-extraction technology of Compound kuhuang formulation was optimized by orthogonal design,with the total contents of matrine and oxymatrine,the total content of prim-O-glucosylcimifugin and 5-O-methylvisamminol,the content of berberine hydrochloride and extract yield,and with the extraction time,amount of water(3 reflux extractions)and soaking time as the factors;and verifi-cation tests were conducted. RESULTS:The extraction time had significant effect on comprehensive evaluation value (P<0.05). The optimal water-extraction technology was as follows as extraction time of 120 min,water amount of 8,6,and 6 times as the amount of herbs,and soaking time of 20 min. In verification tests,the average total content of matrine and oxymatrine was 3.152 6 mg/g(RSD=1.03%,n=3),that of prim-O-glucosylcimifugin and 5-O-methylvisamminol was 4.977 2 mg/g(RSD=2.27%,n=3),the average content of berberine hydrochloride was 3.345 0 mg/g (RSD=1.19%,n=3) and the average extract yield was 49.23%(RSD=2.43%,n=3). CONCLUSIONS:The optimal water-extraction technology of Compound kuhuang formulation is stable and feasible.

OBJECTIVE:To develop a new electronic medication record web page and facilitate its remote data management.METHODS:With the aid of the Windows 2000 platform,the new electronic medication record web page was set up by using Visual Studio.Net,and its remote data management was achieved by using SQL Server.RESULTS:With the new mode of electronic medication record web page,detailed and accurate clinical data become available for clinical pharmacists and a remote data management has come off,furthermore,it is easy to operate.CONCLUSION:As a new mode of electronic medication record,it deserves to be popularized in clinical pharmacists.

OBJECTIVE: To evaluate the extent of endolymphatic hydrops as shown by three-dimensional fluid-attenuated inversion recovery magnetic resonance imaging (3D FLAIR MRI) performed 24 hours after bilateral intratympanic gadolinium administration and discuss the positive rate of endolymphatic hydrops in vestibule and cochlea. METHOD: Twenty-four hours after bilateral intra-tympanic 8 times diluted gadolinium administration, three-dimensional fluid-attenuated inversion recovery MRI, using a three-Tesla unit, was performed in 48 patients, and then assessed the extent of endolymphatic hydrops in the MRI images. RESULT: Forty-eight patients showed different levels of enhancement of perilymth in the inner ear. In these patients, obvious signs of endolymphatic hydrops were visualized in vestibule,including 8 slight hydrops, 17 mild hydrops and 23 severe hydrops with a diagnostic rate of 83.3%. There were almost no complications in all 48 patients after bilateral intra-tympanic injection except short vertigo in some of them. CONCLUSION 3D FLAIR MRI resonance imaging has a high positive diagnostic rate in assessing endolymphatic hydrops of Ménière's disease and provides direct imaging evidence for diagnosing Ménière's disease.
Cochlea ; Contrast Media ; Ear, Inner ; Ear, Middle ; Gadolinium DTPA ; Humans ; Imaging, Three-Dimensional ; Injections ; Magnetic Resonance Imaging ; Meniere Disease ; diagnosis ; Vestibule, Labyrinth