Objective To evaluate the outcomes of microdiscectomy for treatment of recurrent lumbar disc hemiations. Methods There were 18 cases of recurrent lumbar disc berniations accepted microdiscectomy (reoperation group), compared with 30 cases of primary lumbar microdiscectomy (primary group). Operating time, blood loss and length of hospital stay were compared between two groups. MacNab criteria were used to evaluate the outcomes. Results Operating time was a mean of 75 min in reoperation group, 52 min in primary group. Blood loss of reoperation group was 70 ml, 50ml in primary group. Length of hospital stay was 8.5d in reoperation group, 6.5d in primary group. There were one dural tear, two Ls root lesions and 5 more numbness reoperation group, but recovery all patients. There were one dural tear in primary group. 17 cases got follow-up mean 12.1 months in reoperation group and 29 cases in primary group mean 11.8 months. The results demonstrated 88.2% success rate in reoperation group and 93.1% in primary group. There was no significant dif-ference in two groups (P ＞ 0.05). Conclusion Outcomes of Lambar mierodisceetomy with mini-open for treatment of recurrent lumbar disc herniations are similar to primary lumbar mierodiscectomy.

Objective: To evaluate the effectiveness of family interventions among children with allergic rhinitis receiving immunotherapy, so as to provide insights into improvements of allergic rhinitis treatment. Methods: A total of 80 children with allergic rhinitis admitted to The First People's Hospital of Linping District from July 2018 to July 2021 were enrolled and randomly assigned into the control and intervention groups, of 40 participants in each group, and all children underwent immunotherapy. Children in the control group received routine interventions, while participants in the intervention group were given family interventions for 3 months, including health education, psychological counseling, and periodical follow-up. Parental awareness of allergic rhinitis was investigated using self-designed questionnaires, and the compliance to immunotherapy was evaluated. The clinical symptoms were evaluated using the symptom scores and the Rhinoconjunctivitis Quality of Life Questionnaire (RQLQ), and the prognosis was evaluated using the Sino-Nasal Outcome Test-20 (SNOT-20). Results: There were no significant differences between the intervention and control groups in terms of gender, age, course of disease or disease severity (P>0.05). The scores for parental awareness of etiology [(9.56±0.25) vs. (7.45±0.85)], inducement factor [(8.84±0.62) vs. (6.76±1.36)], medication management [(9.56±0.25) vs. (7.97±0.85)] and daily life management of allergic rhinitis [(9.14±0.55) vs. (8.14±0.46)] were significantly higher in the intervention group than in the control group (P<0.05). The proportion of participants' compliance to immunotherapy was significantly higher in the intervention group than in the control group (92.50% vs. 75.00%, P<0.05). The scores for clinical symptoms [(3.12±0.94) vs. (3.96±1.23)], RQLQ score [(3.31±0.87) vs. (3.87±1.02)] and the SNOT-20 scores for nasal symptoms [(6.54±2.14) vs. (8.22±2.45)], sleep disorders [(4.11±0.58) vs. (5.24±1.03)], associated symptoms [(5.29±1.52) vs. (6.34±2.01)] and emotional consequences [(7.52±1.85) vs. (9.19±2.69)] were significantly lower in the intervention group than in the control group post-interventions (P<0.05). Conclusion Family interventions are effective to improve the compliance to immunotherapy, clinical symptoms, prognosis and quality of life among children with allergic rhinitis

Objective To evaluate the susceptibilities of different testing methods of tigecycline against IRAB. Methods MIC and inhibitory zone diameters of 32 strains of IRAB for tigecyclinc were determined by different testing methods.Data were analyzed by comparing the results from each method to those produced by the reference macro broth dilution method. Results The rates of sensitivity intermediate resistance detected by macro broth dilution method , agardilution , MTS and Vitek-2 instrument , disk diffusion method were as follows , 34.375%, 65.625%, 0%, 3.125%, 81.25%, 15.625%, 75%, 25%, 0%, 78.125%, 21.875%, 0%, 3.125%, 81.25%, 15.625%. Compared with macro broth dilution method ,the key major error rate of other methods was 0% by FDA breakpoints , but the CA and EA rates were less than 90%. Conclusions Tigecycline has good activity against the IRAB, with significant difference in susceptible rate among the different methods.

Objective To discuss the influence of respiratory training and prompting effective discharging of sputum for chronic obstructive pulmonary disease (COPD) patients in acute aggravation period. Methods 50 patients were randomized into the intervention group and the control group with 25 cases in each group.The intervention group received respiratory training and prompting effective discharging of sputum while the control was only given routine treatment and nursing.The ratio of first second expiration volume to forced vital capacity, oxygen partial pressure (PaO2),carben dioxide partial pressure (PaCO2) ,blood oxygen saturation (SpO2)and the in-hospital days were compared between the two groups. Results The clinical effect in the intervention group was superior to that of the control group with shorter in-hospital days (P<0.01).Conclusion Better results could be obtained by application of respiratory training and prompting effective discharging of sputum for COPD patients in acute aggravation period.

Objective　To observe rat plasma angiotensin Ⅱ (AⅡ) content change after exposed to infrasound. Methods　Infrasound was exposed to rats for 1,7,14,21 d and two hours every time in a day. After half, six, twelve, eighteen and twenty-four hours, AⅡcontent change was observed. Results　After exposed to infrasound for 1 d,AⅡcontent continued to increase in the next twenty-four hours compared to control group (P<0.01).After infrasound exposure for 7 d,AⅡ began to increase after half hour compared to control group(P<0.01), reached maximum at twelve hours, then began to decrease and turned less than that in the control group at twenty-four hours(P<0.05).After infrasound exposure for 14 d, AⅡ increased significantly compared to the control group(P<0.01).At six hours and twelve hours,there was no significant difference(P>0.05),it increased notably at eighteen hours and twenty-four hours(P<0.01).After infrasound exposure for 21 d, AⅡ began to increase at half hour compared to the contol group(P<0.05),reached maximum at twelve hours,then began to decrease and showed no significant difference at twenty-four hours(P>0.05).Conclusion　After infrasound exposure, there was irregular change of rat plasma AⅡ content at different times.

Objective To evaluate the primary outcomes of minimally invasive lumbar microdiscectomy. Methods There were 34 cases with single lumbar disc herniation treatment of tubular retractor access with METRx system and microdiscectomy(MMD group)and same cases with treatment of conventional discectomy(CD group).Operating time,blood loss,time of leaving the bed and length of hospital stay were compared in two groups.The postoperative wound pain was measured using a visual analog scale(VAS).The modified MacNab criteria were used to evaluate the outcomes. ResuIts The mean operating time was 65 min in MMD group,63 min in CD group.The mean blood loss was 34 ml in MMD group,85 ml in CD group.To compare in two groups,there was significant difference(P＜0.05).The mean time of leaving the bed after operation was 3.2 d in MMD group.5.8 d in CD group.The mean length of hospital stay was 5.8 d in MMD group.9.6 d in CD group.The mean VAS of the postoperative wound pain was 3.2 scores in MMD group,5.6 scores in CD group.To compare in two groups,there was significant difference(P＜0.05).There was one complication in MMD group,4 in CD group.Thirty-three cases got follow-up mean 8.5 months in MMD group.The success rate was 93.9%. Conclusion The minimally invasive lumbar microdiscectomy has the advantages on less trauma,safety,quickly postoperative recovery,and satisfactory outcomes.

SF-36 scale has been widely accepted because of its objectivity and scientificity. However, until now its application in TCM treatment for rheumatoid arthritis is not common. This article discussed the significance of SF-36 scale as the main content of life quality assessment in the efficacy evaluation system of TCM treatment for rheumatoid arthritis, which can provide certain references for the promotion of the application in this field.

Objective:To study the effects of periodontal treatment on the levels of serum lipids and lipoprotein associated phospho-lipase A2(LP-PLA2)in patients with chronic periodontitis(CP)and metabolic syndrome(MS).Methods:52 cases of CP with MS were included,the blood lipid levels,clinical periodontal indexes and white blood cell count(WBC)were detected before and 3 months after treatment.Results:Before and after periodontal therapy the levels of AL(mm)were 5.02 ±0.68 and 3.61 ±0.43(P<0.01),PD(mm)4.07 ±0.46 and 2.52 ±0.39(P<0.01),BOP positive loci(%)92.13 ±6.98 and 37.41 ±8.19(P<0.01), PLI 1.38 ±0.29 and 0.89 ±0.27(P<0.05),TG(mmol/L)1.99 ±0.42 and 1.45 ±0.32(P<0.01)and TC(mmol/L)6.11 ± 0.38 and 5.17 ±0.41(P<0.01),HDL(mmol/L)1.06 ±0.22 and 1.41 ±0.19(P<0.05),respectively.Before and after treat-ment WBCs(×103/L)were 6.03 ±0.42 and 5.52 ±0.37(P<0.01),serum LP-PLA2(mg/L)31.02 ±9.81 and 23.89 ±14.15 (P<0.01),respectively.Conclusion:Periodontal therapy can improve the blood lipid levels in patients with CP and MS.

Objective To develop new methods to cultivate, retrieve and purify oligodendrocyte progenitor cells (OPCs). Methods Primary OPCs were isolated from mixed glial cultures of newborn Sprague Dawley rat forebrains by using a selective detachment procedure, further purified by differential adhesion, and maintained in a chemical conditioned medium. The characteristic of OPCs was determined by both electrophysiological recording and immunostaining. Results Immunocytochemical analysis revealed that more than 90% of the isolated cells expressed the OPCs-specific marker NG2. OPCs exhibited a moderate input resistance, delayed rectifier K+ currents (IK) that could be antagonized partly by TEA-Cl and CsCl, small tetrodotoxin (TTX) sensitive Na+ currents that failed to generate typical action potentials. Conclusion Isolation and purification by selective detachment and differential adhesion procedure are effective to obtain highly purified OPCs. OPCs, distinct from neuron, astrocyte, oligodendrocyte or microglia, possess unique physiological properties.

Objective To observe the postnatal development and perinatal electrophysiological characteristics of oligodendrocyte precursor cell (OPC) in rats. Methods Immunohistochemistry and Western blot were applied to determine the expression of NG2 OPC in cerebral cortex and hippocampus at various developmental stages of SD rats. Electrophysiological characteristics of OPC were also recorded in slices of 7-day rats. Results The majority of hippocampal and cerebral OPC exhibited stellate shape,a small cell body with a few processus. Total population of the NG2 immunopositive OPC was numerous at P7d in cerebral cortex and hippocampus. OPC expressed in adult rats with slightly more quantity. Moreover,OPC in hippocampus of P7d rats typically exhibited small inward sodium current and weak active responses,whereas only outward potassium current and inactive responses were recorded in white matter OPC of P7d rats. Conclusion Total population of OPC and relative optical density of NG2 are the highest in P7d rats at the postnatal developmental stages. OPC in cerebrum and hippocampus of P7d rats displays electrophysiological heterogeneity.