Objective To investigate the value of the high performance liquid chromatography (HPLC) with fluorescence detection of gastric juice in the diagnosis of gastric carcinoma. Methods After being super centrifuged, the supernatant of the gastric juice was chromatographed by reversed phase HPLC using C 18 column as the stationary phase and 20% methanol/H 2O as the mobile phase. The flow rate was set to 1 ml/min. The eluate was then detected with a fluorometer. Results 251 patients with different gastric diseases including 40 cases with gastric carcinoma were enrolled in our study. There were two characteristic peaks in the chromatograms, which appeared in both the benign and malignant lesions. The difference between benignancy and malignancy was the areas of the two peaks. The areas in the malignancy were much larger than those of the benignancy ( P

Objective To investigate the morbidity and Helicobacter pylori (H.pylori) reinfection status within 5 years after eradication. Methods One thousand and six adults aged between 35 to 37 were selected from general population in the high incidence region of gastric cancer. Among them, 552 individuals were confirmed to be H.pylori positive by both CLO test and Warthin Starry Stain histology. All the H.pylori positive individuals were randomly divided into two groups: treatment group receiving OAC triple therapy for one week and placebo group as controls. One month after cessation of treatment, the H.pylori eradication rate was 88.9%, while in the control group the H.pylori infection rate still remained 96.4% by 13 C UBT. These subjects were endoscopically followed up at the end of first and fifth years, respectively. H.pylori infected status was studied by histology and CLO test. Results 1. At 1 and 5 years after eradication, the response rates of subjects were 89.13% and 83.11%, respectively. 2. The morbidity rates of peptic ulcer, before treatment, were 9.87% and 7.61% in the treatment group and control group, respectively. At the 1st year, the morbidity rates were 3.70% and 12.85% in the two groups ( P

Objective To discuss Zedoray Rhizome on the correlation between its traditional Chinese herbal nature(Property and Flavor,Cannel Tropism,Efficacy and so on)and its pharmacologic actions.Methods By searching for and summarizing the literatures on the nature and pharmacologic actions of Zedoray Rhizome recorded in CNKI,CBM,Wanfang and Vip Database from 1989 to 2007,analyzed the correlation between its herbal nature and its modern pharmacologic actions.Results The efficacies of"Qi-promoting Blood-breaking and stagnation-removing" are related with the modern pharmacologic actions of resisting tumor,improving factors of hemorheology,resisting inflammation and virus,and preventing acute liver injury,etc;the efficacy of "Warming middle energizer to relieve pain"means the function of improving activities of spleen,stomach and uterus in modern pharmacology;and the spleen channel tropism is related with and its pharmacologic action of enhancing immune activity.Conclusion There are some correlations between Zedoray Rhizome's traditional Chinese herbal nature and its modern pharmacologic actions.The correlations between Zedoray Rhizome's traditional Chinese herbal nature and its modern pharmacologic actions are waiting for the further improvements to provide the dependable basis for the clinical application.

Objective The aim of this study was to assess the reliability of a newly developed enzymeimmumoassay, the Helicobacter pylori (H. pylori) stool antigen (HpSA) test, for the detection of H.pylori infection before and after eradication. Methods The H.pylori infected patients referred to our department were included. They were divided into two groups. The 331 patients in group A had intact stomach, and 65 patients in group B had history of subtotal gastrectomy. All patients underwent gastroscopy with biopsies for rapid urease test (RUT) and histology, which was viewed as “gold standard”. H.pylori status was defined as positive or negative with both RUT and histology presenting concordant positive or negative results. The results of these reference tests were compared with those obtained by HpSA test and ()~(13)C-urea breath test (()~(13)C-UBT). In addition, Fifty-six-positive patients in group A, constituting group C, were treated with 1-week triple therapy. At the 28 th day after the end of therapy, the patients underwent another ()~(13)C-UBT which was also defined as “gold standard”. The stool specimens were collected on days 1, 7, 14, 21, and 28 after completion of therapy and were used to detect the antigen of H.pylori by HpSA. Results In group A, 175 patients were defined as H.pylori-positive and 156 as negative by the “gold standard”. The sensitivity and specificity of the HpSA test was (95.4%) and 91.0%, respectively. There was no significant difference between HpSA test and ()~(13)C-UBT. In group B, 30 patients were defined as H.pylori-positive and 35 as negative by the “gold standard”. The sensitivity of the HpSA test and ()~(13)C-UBT was 90.0% and 66.7%, respectively (P

Objective To investigate the prevalence of gastric cancer and the relationship between H.pylori infection and the histopathologic changes of gastric mucosa in patients who had their H.pylori eradicated for 8 years in the high incidence region of gastric cancer. Methods One thousand and six adults(age from 35 to 75 years old)were randomly selected in Yantai area, Shandong province in 1996. Five hundreds and fifty two individuals with H.pylori positive were randomly divided into treatment group (n=276) and placebo group(n=276). Treatment group received triple therapy (omeprazol, amoxycillin and clarithromycin) for one week. The placebo group was served as control. One month after completion of treatment, H.pylori status was reassessed by 13C-UBT. Eradication rate of H.pylori in the treatment group was 89%.Follow-up study was carried out with endoscopy in the 2nd, 5th and 8th year, respectively. The endoscopic appearance and histology of the biopsies from the same site obtained at the first and final endoscopy were compared. Results ①At the 8th year′s follow up, 7 patients deve-loped gastric cancer, 1 in the H.pylori negative group(n=246)and 6 in the H.pylori positive group(n=306). The prevalence was 1/1968 person years in the treatment group and 6/2448 person years in placebo group(P0.05). ③ Eight years after eradication of H.pylori , the proportion of gastric atrophy in the body in the H.pylori eradication group was much lower than that in the non-eradication group(P0.05). Conclusions H.pylori infection increased the risk of gastric cancer occurrence, H.pylori eradication is able to reduce such risk. H.pylori eradication results in reduction in the pre-valence of gastric atrophy in the body, while continued H.pylori infection leads to progressive aggrava-tion of gastric atrophy and intestinal metaplasia.

Objective To investigate and analyze the health education requirement in migrant-worker patients and establish health education countermeasures that accord with migrant-worker pa-tients. Methods 600 hospitalization patients were chosen from January to December, 2007,among which 300 migrant-worker patients were set as the experimental group,300 native patients were selected as the control group.The contents and manners of health education requirement were compared between the two groups. Results In the aspect of contents of health education requirement,the highest require-ment was to understand treatment expense,which was significantly higher than that of the control group. The higher requirement included to understand illness condition and treatment protocols,prevention and treatment of common disease and infectious disease.In the aspect of manners of health education require-ment,the highest requirement was to communicate with medical workers,the lowest requirement was to communicate among patients. Conclusions Migrant-worker patients concern about treatment expense, nurses should not only conduct health education about disease-related knowledge, but also pay much atten-tion to informed consent about treatment expense in order to avoid unnecessary dispute.

Objective To observe the effectiveness of electrolyzed oxidizing water(EOW) in disinfections of endoscopes and compared with that of 2% glutaraldehyde. Methods (1) Disinfections of bacteri-a:Specimens were obtained immediately from endoscopes after being used, disinfected and washed out for bacterial culture. Endoscopes were immersed in either EOW or 2% glutaraldehyde for 1 , 3 and 5 minutes. Each time group consisted of 30 cases. (2 ) Effectiveness on disinfections of H. Pylori: endoscopes either from patients with H. Pylori infection or artificially contaminated with H. Pylori were washed out and exposed for 1 ,3 and 5 minutes in each of the disinfectants. PCR was used to detect DNA of H. Pylor. (3) Effectiveness of disinfections of HBV : Endoscopes were contaminated with HBV-positive serum, and the exposure time to both disinfectants were 1,3,5 and 10 minutes respectively. Thirty samples were studied in each time groups. Results ( 1 ) After manual cleaning and disinfections for 1, 3 and 5min in EOW, no vegetative bacteria were found. In 2% glutaraldehyde processing group, 5/30, 4/30 and 1/30 specimens remained bacteria positive, respectively. (2)There was no positive specimens found after 3 and 5 minutes' exposure in EOW in the group of H. Pylori, but 2/30 positive specimens were found in the 1 minute group. While 3/30, 4/30 and 4/30 positive specimens were found after exposure in 2% glutaraldehyde. (3) In the group of HBV, there is no positive case found after exposure in EOW, while in 2% glutaraldehyde groups, there were 2,5 ,4 and 5 cases remained positive in 1 ,3,5 and 10 minutes group respectively. Conclusion EOW is superior to 2% glutaraldehyde in disinfections of Hp or HVB with high-speed and thoroughness, and worthy to be recommended in disinfections of endoscopes.

Electrocardiograph(ECG)teaching is always one of the emphases and difficulties in diagnostics teaching.And this article summarizes the experience on ECG teaching reform by using computer assisted instruction(CAI).

Objective To investigate some specific fluorescent substances in gastric juice from patients with gastric carcinoma and to establish biomarkers for the detection of gastric cancer. Methods We collected gastric juice from 10 patients with gastric carcinoma and 10 patients without gastric carcinoma respectively. After being condensed, the specimens of gastric juice were analyzed by means of high performance liquid chromatography (HPLC). We collected the specific fluorescent substance in the retention time of 11.5-12.5 min in fluorescence spectra of HPLC and then detected them through mass spectrometry. Comparing such spectra with those of L-tryptophan, we attempted to find the principle component of the fluorescent substances. Results There was a specific substance in gastric juice in the retention time of 11.5-12.5 min in fluorescent spectra of HPLC, which was more abundant in gastric malignancy than in gastric benigh diseases. With the mass charge ratio(m/z)of 205, the substance had the same mass spectrum as shown in the third level of L-tryptophan, which was confirmed by mass spectrometry. This substance was identified to be L-tryptophan. Conclusions L-tryptophan is one of the specific fluorescent substances in gastric juice from patients with gastric carcinoma.

Objective: To study the effect of recombinant human intestinal trefoil factor ( rhITF) on the healing of rat chronic gastric ulcer , protect gastric mucosal and mechanisms are involved. Methods: (1) Acute gastric mucosal injury was induced by ethanol, stress, aspirin and pylorusl ligation. The injury index ,MDA, GMBL,hexosamine (Hex) and acid output were measure. (2) Chronic gastric ulcer was induced in rats by application of 50% glacial acetic acid to the serosa of the glandular stomach. After injury, rats received by rhITF or vehicle orally twice daily for 11 days. On day 12, gastric mucosal blood flow(GMBF)was measured under ether anesthesia. Then the pylorus was ligated for 3 hours and each stomach removed. The gastric acid output, ulcer index, Hex and nitric oxide(NO) content in gastric mucosa, as well as iNOSmRNA in the ulcer bed were determined. Results: (1) rhITF protected gastric mucosa from the acute lesion, and increased Hex content in gastric mucosa. (2) rhITF treatment significantly decreased the ulcer index and gastric acid output, but increased the GMBF, Hex and NO content in comparison with the control groups. In addition, rhITF also stimulated iNOSmRNA expression in the ulcer bed by situ hybridization analysis. Conclusion: rhITF can protect gastric mucosa against acute lesion, and enhance the healing of chronic gastric ulcer in the rats.This action may result from the inhibition of gastric acid output, increase of GMBF.Hex and NO content and rhITF stimulated iNOSmRNA expression.