1.Effect of Rehabilitation Gymnastics with Music on Postlaparoscopic Pain Syndrome
Chunlan WU ; Meizhen WEN ; Xiaoyun DENG ; Lixiu LEI ; Shi LIAO ; Xiaoqing ZHENG ; Cuilan MA
Chinese Journal of Rehabilitation Theory and Practice 2009;15(6):591-592
Objective To observe the effect of rehabilitation gymnastics with music on non-incisional pain of gynecological patients after laparoscopic operation.Methods 192 gynecological patients undergone laparoscopic operation were divided into the music group (66 cases, adopted rehabilitation gymnastics matching music after operation), non-music group (64 cases, adopted rehabilitation gymnastics) and routine group (62 cases, adopted routine nursing without rehabilitation gymnastics or music).Results The patients of the music group got pain eased more obviously than those of the non-music group and rule group ( P<0.05).Conclusion The rehabilitation gymnastics with music can relieve non-incisional pain after gynecological laparoscopic operation, and improve anus exhausting.
2.Induced pluripotent stem cell-derived exosome attenuates the inflammatory response in microglia
Lixiu MA ; Ce XIAO ; Zhizhe ZHANG ; Yi'an ZHAN
Chinese Journal of Emergency Medicine 2023;32(1):52-58
Objective:To investigate the effects of induced pluripotent stem cell-derived exosome (iPSC-Exo) on releasing inflammatory factors from microglia induced by lipopolysaccharide (LPS).Methods:iPSC derived from the tubular epithelial cells of sepsis encephalopathy patients were resuscitated and cultured. The iPSC-Exo was isolated by low-temperature ultracentrifugation and analyzed by transmission electron microscopy, Western blot and high sensitivity flow cytometry (HSFCM). Based on the concentration of iPSC-Exo, human microglia line HMO6 cells activated by LPS (100 ng/mL) were divided into four groups randomly: LPS+ phosphate buffer solution (PBS) group, LPS+iPSC-Exo 10 5 group, LPS+iPSC-Exo 10 6 group and LPS+iPSC-Exo 10 7 group. The control group was added equal PBS but not LPS. After culture for 24 h, the concentrations of malondialdehyde in cells were detected. Quantitative RT-PCR was used to measure the mRNA expression levels of macrophage inflammatory protein 2 (MIP2), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in the cells and enzyme-linked immunosorbent assay (ELISA) was used to assess the concentration of these cytokines in the supernatant. Under the same concentration of iPSC-Exo, one-way ANOVA and SNK- q test were used for comparison between groups. Results:The extracts showed spherical membrane structure by transmission electron microscopy. HSFCM showed the mean diameter of the extracts was (74.66±15.60) nm and the concentration around 2.98×10 10/mL. Western blot analysis showed high expression of exosome markers CD63, Alix and TSG101, but not GM130. Intracellular MDA concentration and mRNA expression levels and protein concentration of MIP2, TNF-α, IL-1β and IL-6 in the LPS+PBS group were significantly higher than those in the control group (all P<0.01). With the increase of iPSC-Exo concentration, the intracellular MDA concentration decreased gradually ( P<0.01), the mRNA expression levels of inflammatory factors showed a gradual downward trend (all P<0.05). Each inflammatory cytokine in the supernatant declined in a manner that was almost consistent with mRNA. Concentrations of MDA remained constant in the control group. Conclusions:iPSC-Exo derived from the tubular epithelial cells of sepsis encephalopathy patients alleviate oxidative stress and inflammation effect of microglia induced by LPS, and the modulatory effect is dose-dependent.