Objective To analyze effect of the exercise reducting and improving fistula complications and the quality of life for continuous hemodialysis patients.Methods 106 patients with end-stage renal failure and hemodi-alysis were randomly divided into all 53 patients in observation group (using yoga exercise intervention)and 53 pa-tients in the control group (no intervention measures).Incidence of venous thrombosis,arterial thrombosis,false aneu-rysm,vascular complications were observed in patients of two groups.The quality of patients′life after dialysis care sit-uation in two groups was evaluated by a simple scale of health survey Mos SF-36.Results The comparison result of complication occurs case showed that the incidence of venous thrombosis,arterial thrombosis end,false aneurysm,vas-cular complications in the observation group were respectively 13.21%,0,3.77% and 20.75%;The same indexes were 33.96%,9.43 %,15.09% and 58.49% in the control group,and the complications in the observation group were significantly fewer than the control group (χ2 =6.33,5.15,3.98,10.44,P<0.01,P=0.02,P=0.04).the improved situation of life quality in the two groups showed physiological function,physical function,bodily pain,g en-eral health,vitality,social function,emotional function were significant differences before and after the mental health care in the observation group (t=5.05,4.57,2.85,6.95,10.06,3.28,6.71,8.11,P<0.01);in addition to the physiological functions in the control group,social function,the other were also statistically significant (t =2.42, 1.27,2.06,4.54,4.76,2.19,1.46,3.83P<0.02,P<0.05,P<0.01).In addition to the social function,other improvements in the observation group were better than that in the control group after the mental health care (t=3.06,3.59,2.02,3.79,7.14,1.74,4.22,2.25,P<0.05,P<0.01).Conclusion Through Yoga intervention continued dialysis,the patients'own immune system and disease resistance are improved,the patient's ability to withstand continuous hemodialysis are also improved,thereby to increase the effect of continued hemodialysis.

Objective To explore the cognitive changes of patients with MCI using the Montreal Cognitive Assessment(MOCA) for elderly preliminarily.Methods 85 normal controls (NC),117 subjects with mild cognitive impairment (MCI) and 73 patients with Alzheimer's disease (AD) were assessed with the MOCA.Results There were significant differences among the three groups in all items of MOCA(F=258.66,P＜0.01).Significant differences were observed in almost all the sub-tests between MCI group and NC group or MCI group and AD group(visuopatial F=54.86,P＜0.01;naming F=17.30,P＜0.01;attention F=82.50,P＜0.01;language F=25,88,P＜0.01;abstraction F=15.00 ,P＜0.01;delayed recall F=130.49,P＜0.01;orientation F=176.09,P＜0.01.).The most significant differences were found in delayed recall and orientation among three groups(F=176.09,P＜0.01;F=130.49,P＜0.01.).At 26 cut-point,The results of screening of the MOCA agree with the gold standard of clinical diagnose for the patient with MCI.The agreement rate for observation was 0.93.The agreement rate by chance was 0.61.The Kappa was 0.85.Conclusions The MOCA appropriately define MCI,NC and AD in their cognitive function,it has good discriminant validity.The test of delayed recall and orientation may be more sensitive in the detection of the older people.The MOCA is a useful screening instrument for the patient with MCI.

Objective To analyze the correlation between critical thinking ability and self- efficacy for nursing students in university of traditional Chinese medicine, so as to provide references and evidences for nursing quality teaching. Methods A total of 291 nursing students in university of traditional Chinese medicine were investigated with Critical Thinking Disposition Inventory- Chinese Version (CTDI- CV) and General Self- Efficacy Scale (GSES). Results The total scores of critical thinking ability and self- efficacy were (288.24±32.95) and (25.44±5.74). There were consistence between critical thinking ability and self- efficacy (r=0.348, P<0.01). Regression analysis revealed that self- efficacy and interpersonal relations were predictors of critical thinking ability. Conclusions There were consistence between critical thinking ability and self- efficacy. Self- efficacy and interpersonal relations were predictors of critical thinking ability. It is suggested to promote critical thinking ability by enhancing self- efficacy and interpersonal relations, which contributes to improvement of comprehensive quality.

Objective To establish intellectual critique of nursing thesis course in postgraduate nurse,and to evaluate teaching effectiveness and the reasonableness of the course.Methods A total of 44 postgraduate nurses were involved in the study,which were carried out through action research of planning,acting and observing,and reflecting.After the course,researchers evaluated the reasonableness of the course and teaching effectiveness through the self-control.Results There was significant difference in the scientific research ability score of research object before and after the course:(60.09±16.26) scores vs.(71.27±13.22) scores,t=-3.264,P＜0.01.But there was no significant difference in the critical thinking ability scores of research object before and after the course:(295.55±29.71) scores vs.(292.71±26.67) scores,t=0.478,P＞0.05.Conclusions The course of intellectual critique of nursing thesis can improve the research ability of postgraduate nurse,but the role of the critical thinking ability remains to be discussed.And the course needs to be further improved.

Objective To study the role of hydrogen sulfide (H2S) in the effect of SB203580 on proliferation and apoptosis of hepatic stellate cells and the effects of H2S on expressions of collagenⅠand collagenⅢmRNA in hepatic stel-late cells. Methods There were five groups of HSC-T6 cells in this study including control group (DMEM medium contain-ing10%fetal bovine serum), dimethyl sulfoxide (DMSO) group, sodium hydrosulfide (NaHS)group,SB203580 (SB)group and SB+NaHS group. MTT method was used to detect the cell proliferation and inhibition rate of HSC-T6 cells treated by SB203580 and H2S. The apoptotic rate of HSC-T6 cells was detected by flow cytometry with annexin V-FITC/PI double staining. RT-PCR was used to detect the expressions of collagenⅠand collagenⅢmRNA in HSC-T6. Results The apop-totic rate of HSC-T6 cells was significantly higher in SB group and SB+NaHS group than that of control group, and the rate was significantly higher in SB+NaHS group than that of SB group (P＜0.05). There was no significant difference in the apop-totic rate of HSC-T6 cells between DMSO and NaHS groups than that of control group. The expressions of collagenⅠand col-lagenⅢmRNA were found in five groups of cells. There was a higher expression of collagenⅠand collagenⅢmRNA in NaHS group than that of control group (P＜0.05). The expressions of collagenⅠand collagenⅢmRNA were significantly lower in SB group and SB+NaHS group than those of control group and NaHS group (P＜0.05). Conclusion H2S activated P38MAPK signal pathway. And P38MAPK was specifically blocked by SB203580 in HSC-T6 cells, which inhibited the cell proliferation stimulated by H2S and promoted the apoptosis.

Objective:To explore the chain mediating effect of self-efficacy and positive coping styles between nurses′ adversity quotient on work engagement, so as to provide a theoretical reference for nursing managers to promote work engagement.Methods:A convenience sampling method was used to select 512 nurses from four tertiary hospitals in Shaanxi Province, and a cross-sectional study was conducted on them using the general information questionnaire, Adversity Response Profile, General Self-Efficacy Scale, Simplified Coping Style Questionnaire and Utrecht Work Engagement Scale-9.Results:The total scores of nurses' adversity response profile, self-efficacy, positive coping style and work engagement were 132.00(120.00, 139.00), 27.00(23.00, 29.00), 24.00(20.00, 25.00), 27.00(25.00, 35.00) points. Nurses' work engagement was significantly positively correlated with adversity quotient, self-efficacy and positive coping styles( r values were 0.523-0.614, all P<0.01). The independent mediation of self-efficacy or positive coping styles and the chain mediation of both accounted for 23.6%,40.9% and 15.9% of the total effect respectively. Conclusions:Nurses′ adversity quotient not only directly affected work engagemnet, but also indirectly affected work engagement through self-efficacy and positive coping style. The research suggested that managers should pay attention to the improvement of nurses′ self-efficacy and positive coping style, so as to promote nurses′ work engagement.

Objective To discuss effect of mechanical oiling and manual oiling in rust protection of coupling surgical instruments.Methods A total of 68 surgical instruments were divided into observation group and control group,with 34 surgical instruments in each group.The control group was treated with mechanical oiling,and the observation group used manual oiling,cleaning qualified rate,the rust rate after 3 months,lubricant amount and the average cost per month were observed.Results The qualified rate of surgical instruments by visual inspection in the control group was 99.09％,and was 98.77％ by magnifier,and the observation group were 99.23％ and 98.89％,respectively,but there was no significant difference between the two groups (P ＞ 0.05).Rust rate after 3 months was 8.94％ in the control group,and was 1.40％ in the observation group,there was significant difference between two groups (P ＜ 0.05).Lubricating oil amount and total cost of two groups in the control group were (28 800.00 ±618.96)mL,(12 180.00±256.78) RMB,and were (5 600.00 ±489.71) mL,(2 216.00 ± 189.93) RMB,respectively,in the observation group,and there was significant difference between two groups (P ＜ 0.05).Conclusion Compared with mechanical oiling,manual oiling has better lubrication effect and can significantly reduce cost and corrosion of equipment,improve the maintenance effect,and ensure the quality of disinfection equipment.

Objective To discuss effect of mechanical oiling and manual oiling in rust protection of coupling surgical instruments.Methods A total of 68 surgical instruments were divided into observation group and control group,with 34 surgical instruments in each group.The control group was treated with mechanical oiling,and the observation group used manual oiling,cleaning qualified rate,the rust rate after 3 months,lubricant amount and the average cost per month were observed.Results The qualified rate of surgical instruments by visual inspection in the control group was 99.09％,and was 98.77％ by magnifier,and the observation group were 99.23％ and 98.89％,respectively,but there was no significant difference between the two groups (P ＞ 0.05).Rust rate after 3 months was 8.94％ in the control group,and was 1.40％ in the observation group,there was significant difference between two groups (P ＜ 0.05).Lubricating oil amount and total cost of two groups in the control group were (28 800.00 ±618.96)mL,(12 180.00±256.78) RMB,and were (5 600.00 ±489.71) mL,(2 216.00 ± 189.93) RMB,respectively,in the observation group,and there was significant difference between two groups (P ＜ 0.05).Conclusion Compared with mechanical oiling,manual oiling has better lubrication effect and can significantly reduce cost and corrosion of equipment,improve the maintenance effect,and ensure the quality of disinfection equipment.

To prepare carboxyl terminus truncated human papillomavirus type 58 L1 (HPV58L1) protein and evaluate its ability to form virus-like particles, the baculovirus and Sf-9 insect cells was used to express HPV58L1 protein, and pFastBac-Htb containing HPV58L1 gene sequence of carboxyl terminus truncation was generated. Then Sf-9 cells were infected with recombinant baculovirus. After being cultured, the post-infected cells expressing--HPV58L1 protein were harvested and analyzed by SDS-PAGE and Western blot. The ProBond purification system was used for protein purification. The bio-activity of purified protein was identified by mouse erythrocyte hemagglutination assay, and the VLP formation was examined with transmission electron microscope. Our results showed that the recombinant baculovirus was generated and the Sf-9 cells was infected with the recombinant baculovirus, and after collecting, total cellular proteins were extracted. Truncated HPV58L1 protein with MW 58KD was revealed by SDS-PAGE and confirmed by Western blot. The purified L1 proteins under native condition could cause mouse erythrocytes to agglutinate and form VLP. It is concluded that HPV58L1 protein with carboxyl terminus truncation could be efficiently expressed. In baculovirus Sf-9 cells expression system, the purified protein could self-assemble into virions in vitro, and induce agglutination of mouse erythrocytes, indicating that carboxyl terminus truncation does not interfere with the bioactivity of HPV58L1 protein.
Baculoviridae ; genetics ; metabolism ; Capsid Proteins ; Carbon Dioxide ; Humans ; Inclusion Bodies, Viral ; ultrastructure ; Oncogene Proteins, Viral ; biosynthesis ; genetics ; immunology ; Papillomaviridae ; physiology ; Recombinant Proteins ; biosynthesis ; Virion ; growth & development ; physiology ; Virus Assembly