Objective:To study the relationship between junior middle school students' diathesis and life stress with mental health.Methods: 259 junior middle school students were administrated General Health Questionnaire(GHQ-12), General Self-Efficacy Scale(GSES), Simplified Coping Style Questionnaire, and Adolescent Self-Rating Life Events Check List(ASLEC). Results: (1) Self-efficacy and positive coping styles were positively correlated with mental health,whereas negative coping styles and life stress were negatively correlated with mental health. (2) Self-efficacy, coping styles and life stress directly affect students' mental health. Conclusion: Diathesis and life stress jointly affect students' mental health.

Objective:To study the relationship between perfectionism and interpersonal problems as well as loneliness of college students.Methods:638 college students were tested with Perfectionism Self-Presentation Scale(PSPS),Inventory of Interpersonal Problems(IIP-32)and UCLA Loneliness Scale(UCLA).Results:There was significant correlation between three kinds of perfectionism and interpersonal problems as well as loneliness.And the three kinds of perfectionism could well predict interpersonal problems(Need to appear perfect:?=-0.089;need to avoid appearing imperfect:?=0.268;need to avoid disclosing imperfections:?=0.287).Need to appear perfect and interpersonal problems could predict loneliness(?=-0.114,?=0.605).Furthermore,need to appear perfect,need to avoid appearing imperfect and need to avoid disclosing imperfections could influence loneliness via interpersonal problems.Conclusion:The need to appear perfect could be beneficial to relieve interpersonal problems and loneliness.While the need to avoid appearing imperfect and the need to avoid disclosing imperfections could increase interpersonal problems and loneliness.Interpersonal problems could serve as moderator between perfectionism and loneliness.

ObjectiveTo introduce a rapid and stable rat model of middle cerebral artery occlusion (MCAO). Methods32 Wistar male rats were used to induce focal cerebral ischemia according to Longa's method. The main difference from Longa's was to introduce a nylon suture into the cervical internal carotid artery without ligation the pterygopalatine artery (PPA). Regional cerebral blood flow (rCBF), neurological deficit, brain water content, red tetrzolium (TTC) stain and pathology were evaluated in all rats.ResultsThe operation proceeded about 15 min after anaesthesia. Rats after operation presented severe neurological deficits companied with rCBF decreases. There were significant increases of brain water contents in ischemic brain regions. TTC staining showed infarct areas and histological examinations revealed remarkable astrocyte swelling and neuronal necrosis.ConclusionInducing rat MCAO without ligation of PPA could produce reliable ischemic changes,with the advantage of shortening operation time.

Objective To investigate the effect of vitamin D on pulmonary function in patients with chronic obstructive pulmona-ry disease,and to explore its possible mechanism.Methods Patients with chronic obstructive pulmonary disease admitted in the hospital were randomly divided into 2 groups,one group of patients were given regular COPD treatment (control group,30 cases), the other group of patients were given vitamin D on the basis of conventional therapy(experimental group,30 cases).Pulmonary function test and C reaction protein (CRP)determination in the 2 groups were performed,the contents of PaO2 ,PaCO2 were also determined.Results Compared with the control group,lung function improvement in experimental group were more obvious,and the the number of acute attack significantly reduced(P <0.05).The concentration of CRP in experimental group was significantly lower after treatment than before treatment(P <0.05),while the concentration of CRP in control group didn′t change significantly (P >0.05).Conclusion Vitamin D contributes to the improvement of pulmonary function in patients with COPD,and its mecha-nism might relate to the reduction of inflammatory reaction.

Objectives To conduct nutritional risk assessment analysis of hand-foot-mouth disease in children and to explore rational and feasible nutritional intervention. Methods The information of children with hand-foot-mouth disease in three hospitals in Guilin area was collected by ifxed-spot continuous sampling. Inquiry investigations, physical mea-surements combined with clinical diagnosis were undertaken, and nutritional risk assessment and management were con-ducted by the malnutrition screening tool of children (STAMP scoring). Those with STAMP scores ≥4 were considered at high nutritional risk. Nutritional risk reassessment was conducted on children with high nutritional risk after nutritional intervention and management. Results A total of 192 children with hand-foot-mouth disease were selected. The occur-rence rate of high nutritional risk was 68.75%(132/192). The hospitalization time of patients with high nutritional risk was prolonged and the hospitalization expense was increased apparently. The nutritional support treatment and nutritional edu-cation were undertaken on the children with high nutritional risk. The incidence of high nutritional risk from admission to discharge decreased from 68.75%to 36.98%(P<0.05). The clinical outcomes of those children accepting nutritional inter-vention were signiifcantly improved (P<0.05). Conclusions The occurrence and prognosis of hand-foot-mouth disease is closely related to the nutritional status of children, so nutritional risk assessment, nutritional intervention and management should be performed as early as possible.

Objective To investigate the status of pregnancy syphilis screening ,diagnosis,treatment,follow-up in Panyu district for improvement of management of pregnancy syphilis .Methods Using the method of a retro-spective survey,the follow-up data for 219 cases of patients were diagnosed with gestational syphilis from 2011.10.1 to 2013.9.30.Results 219 cases were diagnosed with gestational syphilis in 103 665 cases of pregnant women .The positive rate was 2.1%and all cases were latent syphilis .176 cases underwent a course of treatment during pregnancy , of which 33 patients received two courses of standard treatment .40 cases didn't receive treatment and 3 cases could not be followed up due to the phone empty ,shut down or refuse .14 cases were congenital syphilis ,the rate was 6.4%. Conclusion Pregnancy syphilis is a serious mother to child transmission of disease .After standard treatment ,close follow-up,strengthen publicity and education to improve patient compliance can reduce the incidence of congenital syphilis.

Objective: To investigate moderating role of sense of humor to the relationship between stressful events and mental health. Methods: 836 middle-school students were investigated by MSHS, ASLEC and GHQ-20. Results: Sense of humor had a positive effect on students’ mental health, and strong humor could deduce the level of anxiety and depressed emotion. Sense of humor could buffer the relationship of stressful events and depression, but it could not adjust the relationship of stressful events with self-affirmation and anxiety. Conclusion: Sense of humor has a moderating effect on the stressful events and some aspects of mental health.

Diabetic keratopathy (DK) is a common ocular complication of diabetes.Long-term hyperglycemia impairs many structures of the cornea, leading to corneal opacity and visual dysfunction.A large number of researches focus on the epithelium and nerve abnormities in DK, but the pathogenesis is not completely elucidated.Dendritic cells (DCs) are specialized antigen-presenting cells, linking innate and adaptive immunity, participating in the occurrence and development of diabetes and its complications.To date, there are many myths in relationship between DCs and DK to be solved, and there are a few researches that investigate the relation between DCs and the occurrence and development of diabetes.In this article, the pathogenesis and pathogenic changes of DK, the types and functions of different DCs, the relationship between DCs and chronic inflammation and delayed healing of corneal epithelium in DK, as well as the role of DCs in corneal neuropathy were reviewed in order to provide some references for further investigations about the pathogenesis and treatment of DK.

Objective To investigate the efficacy and safety spinal ropivacaine and fentanyl-epidural anes-thesia for the first stage of preclinical of childbirth.Methods 78 cases of full-term maternal were divided into the three groups randomly by using digital representation,each group had 26 cases.A group did not implement analgesia, B group was given analgesia in the first stage of incubation(ropivacaine and fentanyl epidural anesthesia),C group was given labor analgesia on the first line of the active phase.The labor time,mode of delivery,Apgar score,pain VAS score and the occurrence of adverse reactions of three groups were observed.Results The VAS pain scores of B group were (5.01 ±0.61)points,(2.30 ±0.32) points and (1.65 ±0.21) points after 5,10,30min of analgesia, which were significantly lower than those of before(t=6.59,9.62,10.73,all P<0.05) and A group(t=6.76, 9.80,11.02,all P<0.05);The VAS pain scores of C group were (4.92 ±0.57)points,(2.34 ±0.30)points and (1.61 ±0.23)points after 5,10,30min of analgesia,which were significantly lower than those of before(t=9.72, 9.55,10.91,all P<0.05) and A group( t=6.88,9.73,11.30,all P<0.05);The VAS pain scores of B group and C group showed no significant difference(t=2.22,2.08,1.99,all P>0.05) at each time point;The first stage of labor of B group and C group were (327.43 ±31.02)min and (335.62 ±30.18)min,respectively,which were significantly decreased compared with A group(t=7.21,6.90,all P<0.05);The natural birth rate of B group and C group was 80.77%and 84.62%,respectively,which were significantly higher than 57.69%of A group(χ2 =8.43, 8.81,all P<0.05);There was no significantly different among three groups in the incidence of adverse reactions (χ2 =2.23,2.23,all P>0.05).Conclusion Ropivacaine and fentanyl in epidural anesthesia had a preferable effect for the first stage of preclinical and active period of childbirth which can effectively reduce maternal labor pain and improve the natural birth rate and has a fewer adverse reactions.

Objective To investigate the effect and mechanism of exogenous IGFBPrP1 on collagen content in liver tissue. Methods Twenty-four male C57BL/6 wild-type mice were randomly divided into three groups: Control group (n=8), rmIGFBPrP1 2 weeks group (n=8) and rmIGFBPrP1 4weeks group (n=8). Both hematoxylin-eosin (HE) staining and picric acid-Sirius red staining were performed. The protein expression of IGFBPrP1, Collagen Ⅰ , Collagen Ⅲ, FN, TGF-β1, Smad3 andp-Smad2/3 was evaluated by immunohistochemistry or Western blot. Results Exogenous IGFBPrP1 can cause pathological changes in liver tissue. Collagen content was significantly increased by hematoxylin-eosin (HE) staining and picric acid-Sirius red staining (P＜0.05). The protein expression of IGFBPrP1, FN and Collagen Ⅰ was gradually increased after rmIGFBPrP1 injection for 2 weeks and 4 weeks by Western blot (P＜0.01). The protein expression of Collagen Ⅲ was obviously increased in the rmIGFBPrP1 2 weeks group and rmIGFBPrP1 4 weeks group by immunohistochemistry, and the level in the 4 weeks group was higher than that in the 2 weeks group (P＜0. 01). The protein expression of TGF-β1, Smad3 and p-Smad2/3 in liver tissue was significantly increased after rmIGFBPrP1 injection in a time-dependent manner by both immunohistochemistry and Western blot (P＜0. 01).Conclusion Exogenous IGFBPrP1 can cause a marked increase in collagen content and the excessive deposition of ECM through the TGF-β1/Smad3 pathway in liver tissue.