1. Study on the effect of oxidative stress on the cardiac injury induced by MEHP in rats
Zeze WANG ; Yi LIU ; Lixiao ZHOU ; Yu ZHANG ; Xuan HE ; Lijuan ZHAO ; Rong ZHANG ; Huicai GUO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2018;36(7):485-491
Objective:
This study was to investigate the effects of MEHP on isolated rat heart and explore its mechanism.
Methods:
The experiments were performed with Langendorff-perfused rat heart with a Langendorff apparatus. 35 SD rats were used in the experiment and there were 5 rats per group. MEHP at doses of 3.125, 6.250, 12.500 and 25.000 μmol/L were given to the hearts for 25 minutes. Effects of NAC at concentration of 5 mmol/L were evaluated by co-treatment with 12.500 or 25.000 μmol/L MEHP. Data was collected per 5 minutes for 25 minutes. The heart rate, LVDP, LVEDP, dp/dtmax, and dp/dtmin were measured and analyzed using a PL3508 Data Acquisition and Analysis System. 200 waves at least were required each time. LDH contents in heart lavage fluid were determined by photometric assays using the automated biochemical analyzer. A section of the heart tissue was used for histopathological examination. DCFH-DA method was used to detect the levels of reactive oxygen species in different groups of heart tissues.
Results:
There was a concentration dependent decrease of heart rate (
2.Identification Study on Cryptotympana pustulata Ecdysis Based on 3D Depth of Field Synthesis Technology
Lixiao GUO ; Zijing XUE ; Fangjie HOU ; Baohui SUN ; Yuguang ZHENG ; Qian ZHENG
China Pharmacy 2020;31(4):447-451
OBJECTIVE:To establish the rapid field identification method of Cryptotympana pustulata ecdysis. METHODS : 3D depth of field synthesis technology was used to identify 50 batches of C. pustulata ecdysis and its adulterants from the length of beak ,size and protrusion degree of upper labial base ,the protrusion degree of the lower labial base ecdysis and the color of its upper transverse groove ,the number and shape of main and lateral spines on the foot ,significance of abdominal valves ,the number of webs ,the number and shape of side plates ,the number of tergum rings ,terminaliae,etc. RESULTS :Among 50 batches of samples ,S1-S5,S26-S30,S36-S50 were C. pustulata ecdysis;S21-S25 was adulterants of C. pustulata ecdysis after weight gain ;S31-S35 was adulterants of C. pustulata ecdysis after extraction ;S6-S20 were ecdysis from Tibicen flammatus ,C. flammatta,Lyristes pekinensis ,all of which were adulterants. The main distinguishing feature of C. pustulata ecdysis and its adulterants was that abdomen and ventral surface of C. pustulata ecdysis were triangular ,and the abdomen and ventral surface of other species was nearly parallel ;the valve of C. flammatta ecdysis was obvious ,but those of other varieties were not obvious ;the lateral appearance of terminaliae of C. flammatta ecdysis was sharper than those of other species ;there was an acute angle between the front foot accessory thorns and the end thorns of the T. flammatus ecdysis,and an obtuse angle between the front foot accessory thorns and the end thorns of the L. pekinensis ecdysis. CONCLUSIONS :The method is simple ,reliable and suitable for rapid field identification of C. pustulata ecdysis and its adulterants.
3.Rapid evaluation of the early pathogen of severe Chlamydophila psittaci pneumonia by diagnostic bronchoscopy
Jingwen LI ; Shengquan LI ; Na LIU ; Tiantian SONG ; Lixiao ZHAO ; Xueli WANG ; Mengya GUO ; Yamei GAO ; Qiufeng WAN ; Sicheng XU
Chinese Critical Care Medicine 2023;35(1):37-42
Objective:To explore the rapid evaluation of the early pathogen of severe Chlamydophila psittaci pneumonia by bedside diagnostic bronchoscopy, so as to start effective anti-infection treatment before the results of macrogenome next generation sequencing (mNGS) test. Methods:The clinical data of three patients with severe Chlamydophila psittaci pneumonia who were successfully treated in the First Affiliated Hospital of Xinjiang Medical University, the First People's Hospital of Aksu District, and the First Division Hospital of Xinjiang Production and Construction Corps from October 2020 to June 2021 were retrospectively analyzed, including the rapid assessment of early pathogens by bedside diagnostic bronchoscopy and the use of antibiotics to start anti-infection treatment. These patients were successfully treated. Results:The three patients were male, aged 63, 45 and 58 years old, respectively. Before the onset of the penumonia, they had a clear medical history of bird exposure. The clinical manifestations mainly included fever, dry cough, shortness of breath and dyspnea. One case had abdominal pain and lethargy. The results of laboratory examination indicated that the peripheral blood white blood cell count (WBC) of two patients were high [(10.2-11.9)×10 9/L], the percentage of neutrophils increased (85.2%-94.6%) and the percentage of lymphocytes decreased (3.2%-7.7%) in all 3 patients after admission to hospital and entering into intensive care unit (ICU). The procalcitonin (PCT) of 3 patients increased after admission, and still increased when entering ICU (0.3-4.8 ng/L), so did C-reactive protein (CRP, 58.0-162.0 mg/L) and erythrocyte sedimentation rate (ESR, 36.0-90.0 mm/1 h). After admission, serum alanine transaminase (ALT) increased in 2 cases (136.7 U/L, 220.5 U/L), so did aspartate transaminase (AST) in 2 cases (249.6 U/L, 164.2 U/L). ALT (162.2-267.9 U/L) and AST (189.8-223.2 U/L) increased in 3 patients when they entered ICU. The level of serum creatinine (SCr) of 3 patients were normal after admission and entering ICU. The chest computed tomography (CT) findings of 3 patients were acute interstitial pneumonia, bronchopneumonia and lung consolidation, of which 2 cases were accompanied by a small amount of pleural effusion, and 1 case was accompanied by more regular small air sacs. Multiple lung lobes were involved, but mainly one lung lobe. The oxygenation index (PaO 2/FiO 2) of the 3 patients admitting to ICU were 100.0, 57.5 and 105.4 mmHg (1 mmHg ≈ 0.133 kPa), respectively, which met with the diagnostic criteria of moderate and severe acute respiratory distress syndrome (ARDS). All three patients received endotracheal intubation and mechanical ventilation. Under the bedside bronchoscope, the bronchial mucosa of 3 patients were obviously congested and edematous, without purulent secretion, and there was 1 case with mucosal hemorrhage. Three patients underwent bedside diagnostic bronchoscopy, and the evaluation result of the pathogen was that it might be atypical pathogen infection, so they were given moxifloxacin, cisromet and doxycycline intravenously, respectively, and combined with carbapenem antibiotics intravenously. After 3 days, the detection results of mNGS in bronchoalveolar lavage fluid (BALF) showed that only Chlamydia psittaci was infected. At this time, the condition was significantly improved, and PaO 2/FiO 2 was significantly increased. Therefore, the antibiotic treatment scheme remained unchanged, and mNGS only served to verify the initial diagnosis. Two patients were extubated on the 7th and 12th day of admission to the ICU, respectively, while one patient was extubated on the 16th day of admission to the ICU due to nosocomial infection. All 3 patients were transferred to the respiratory ward after the condition was stable. Conclusion:The bedside diagnostic bronchoscopy based on clinical characteristics is conducive to not only the rapid assessment of the early pathogens of severe Chlamydia psittaci pneumonia, but also effective anti-infection treatment before the returning of mNGS test results, which can make up for the lag and uncertainty of the mNGS test results.