In this paper we recommended a new method, using "Silmag" as an absorbent for the determination of riboflavin content in foods fluorometrically. The lowest detecting value was 0.006 micrograms. The coefficient of variation of riboflavin in determining milk powder and the seed of Job's tears(Coix La-chrymalobi L.)was 2.1% and 3.3% respectively,and the recovery of added riboflavin in bread was 102.7, 102.3 and 99.7% with an average value of 101.4%; milk powder was 96.0, 103.0 and 104.2% with an average of 101.1%. Both precision and accuracy of this method were satisfactory. In determining riboflavin by fluorometric method, the sample must be treated by enzyme after hydrolysis. The "Silmag" can be reactivated after using.

BACKGROUND:So far steroid pulse therapy andsurgical decompressionarethe main accepted therapiesfor spinal cord injury, but these methods make no effects on injured neurons. Nerve growth factors and endogenous neural stem cels play a role in theneuronalregeneration and remyelination, which provides a newidea for spinal cord injury treatment.
OBJECTIVE:To explore the effect of the basic fibroblast growth factor on proliferation of endogenous neural stem cels after spinal cord injury and to analyze its relationship with the increased fluoro-gold labeled neurons.
METHODS:Totaly 48 Kunming mice were randomly divided into four groups including normal, spinal cord injury, treatment and sham operation groups. Acute spinal cord injury models were establishedin the spinal cord injury and treatment groups, andthe normal group was subjected to operationthat did not damage the spinal cord. At 2hoursafter regaining consciousness, the treatment group was givendailyinjection of MTPBS containing 25 μg/kg basic fibroblast growth factors and 1% album. And at 7 days, laminectomy was carried out again at the L1segment in the former three groups and a smal piece of sterile gelfoam soaked with fluoro-gold was inserted into the incision. The sham operation group was given no processing. Afterwards, mouse motor behavior was assessed using Rotarod and Platform Hang tests; neurons in the corticospinal and rubrospinal tracts were labeled with fluoro-gold; the number of endogenous neural stem cels positive for nestin was detected by immunohistochemistry method. Besides, the correlation betweenthe number offluoro-gold labeled neurons andthe number ofendogenous neural stem cels was assessed.
RESULTS AND CONCLUSION:The basic fibroblast growth factor could significantly improve the mouse motor behavior after spinal cordinjury. And the number of endogenous neural stem cels was significantly increased after the basic fibroblast growth factor injection, which was related to the increased fluoro-gold labeled neurons.In conclusion, basic fibroblast growth factors play an important role in the proliferation of endogenous neural stem cels after spinal cord injury. Furthermore, endogenous neural stem cels improve locomotive behaviors by encouraging the neuronalproliferation.

Objective To investigate the effect of external abdominal aorta compression on circulation during anesthetic induction in elderly patients. Methods A prospective randomized controlled trial was conducted. Patients with age of 60-75 years old, requiring a general anesthesia for non-abdominal surgery, and with Ⅱ-Ⅲ class of American Society of Anesthesiologists (ASA) physical status classification, and admitted to General Hospital of Chinese People's Armed Police Forces from January to April in 2017 were enrolled. They were divided into abdominal aorta pressure group and control group according to random number method, with 20 patients in each group. In both groups, anesthesia was induced with midazolam, propofol, fentanyl and cisatracurium, and was maintained with propofol, remifentanil and cisatracurium. After successful intubation, the anesthesia machine was changed into mechanical ventilation. The patients in abdominal aorta pressure group were given abdominal aorta pressure 1 minute after induction of general anesthesia with midazolam till 5 minutes after intubation. The mean arterial pressure (MAP), heart rate (HR) and blood oxygen saturation (SpO2) were observed before anesthesia induction, immediately after anesthesia induction, immediately after intubation, 5 minutes and 10 minutes after intubation, respectively. The incidence of hypotension or bradycardia, and usage of ephedrine or atropine were recorded. Results There were no significant differences in MAP [mmHg (1 mmHg = 0.133 kPa): 83.6±4.7 vs. 82.9±4.7], HR (bpm: 67.3±5.9 vs. 65.9±5.7) and SpO2 (0.962±0.007 vs. 0.960±0.009) before anesthesia induction between abdominal aorta pressure group and control group (all P > 0.05). Immediately after anesthesia induction, the MAP and HR in control group were significantly decreased as compared with those before anesthesia induction [MAP (mmHg): 70.0±8.7 vs. 82.9±4.7, HR (bpm): 60.7±6.7 vs. 65.9±5.7, both P < 0.05], and they were also significantly lower than those of abdominal aorta pressure group [MAP (mmHg): 83.1±3.9, HR (bpm): 66.8±4.9, both P < 0.05]. Immediately after intubation, the MAP and HR in control group were significantly increased as compared with those immediately after anesthesia induction [MAP (mmHg): 78.9±7.9 vs. 70.0±8.7, HR (bpm): 67.3±2.7 vs. 60.7±6.7, both P < 0.05], but the changes in MAP and HR in abdominal aorta pressure group were not obvious. During the anesthesia induction period, there was no statistical difference in SpO2 change between the two groups. During induction of anesthesia, no adverse reaction was found in the abdominal aorta pressure group, but 4 patients with hypotension and 2 patients with bradycardia were found in the control group. Two patients with hypotension were treated with ephedrine, and 2 patients with bradycardia were treated with atropine. Conclusion Anesthesia induction of elderly patients with abdominal aorta pressure can help maintain hemodynamic stability.

Objective To observe the clinical efficacy of acupuncture-moxibustion in treating early-stage glandular cystitis. Method Forty patients diagnosed with glandular cystitis by cystoscopy and biopsy were divided into a treatment group and a control group, 20 cases in each group. The treatment group was intervened by acupuncture-moxibustion, while the control group was by intravesical perfusion chemotherapy. The clinical efficacies were compared between the two groups. Result The therapeutic efficacy of the treatment group was significantly superior to that of the control group (P<0.05), and the relapse rate confirmed by using cystoscopy and biopsy was significantly lower in the treatment group than in the control group (P<0.05). Conclusion Acupuncture-moxibustion can enhance the clinical efficacy in treating early-stage glandular cystitis.

Objective:To investigate the value of indocyanine green retention rate at 15 minutes (ICG R15) on postoperative day 3 combined with standard remnant liver volume (SRLV) in predicting the occurrence of liver dysfunction after hepatectomyin hepatocellular carcinoma (HCC).Methods:The clinical data of 61 HCC patients undergone hepatectomy in Xiangya Hospital of Central South University from January 2015 to February 2016 were collected and analyzed.The patients were divided into 2 groups:a normal liver function group (n=40) and a liver dysfunction group (n=21).Univariate analysis was used to evaluate the risk factors for postoperative liver dysfunction.Logistic regression was used to assess the independent risk factors for postoperative liver dysfunction,and the regression equation between independent risk factors and postoperative liver dysfunction was established,The receiver operating characteristic (ROC) curve was used to examine the regression equation and compare the value difference in predicting postoperative liver dysfunction between single and combined independent risk factors.Results:Postoperative liver dysfunction occurred in 21 of the 61 patients,with an incidence rate at 34.4％.There was no significant difference in the time of operation,time of hepatic portal occlusion,volume of tumor and volume of resected liver between the 2 groups (all P＞0.05),but there were significant differences in the ICG R15 on postoperative day 3,intraoperative blood loss and SRLV between the 2 groups (all P＜0.05).The ICG R15 on postoperative day 3,intraoperative blood loss,SRLV were the risk factors for postoperative liver dysfunction.Logistic regression analysis showed ICG R15 on postoperative day 3 and SRLV were the independent risk factors for postoperative liver dysfunction,and the regression equation between independent risk factors and postoperative liver dysfunction was as follows:logit(P)=1.277+0.140×ICG R15 on postoperative day 3-5.125×SRLV.The area under the ROC curve ofICG R15 on postoperative day 3 combined with SRLV was more than that of single ICG R15 and single SRLV.Conclusion:ICG R15 on postoperative day 3 and SRLV are the independent risk factors for postoperative liver dysfunction.The regression equation,which is established by combination of ICG R15 with SRLV,can predict the occurrence of postoperative liver dysfunction.The accuracy of ICG R15 on postoperative day 3 combined with SRLV is better than that of single ICG R15 or single SRLV.

BACKGROUND:It is crucial for us to find an objective and sensitive means for the assessment ofthe severitv and development of dementia,so as to make instant diagnosis and examination of therapeutic effect on patientswith vascular dementias(VD).Event related potential endogenetic eomponent P300 is proved closely correlated with the cognitive management of examinees but not with stimulus property,thereby considered as one of the most valuable and objective index for brain superior psychological activity.OBJECTIVE:To study the role and significance of events related potential P300,providing objective basis for clinical diagnosis and therapeutic assessment for patients with VD.DESIGN:Non-randomized simultaneous controlled explorative study based on patients. SETTING:Neurological department and electromyography department in aprovincial hospital and neurological department in a medical college hospital.PARTICIPANTS:Between February 2000 and June 2001,64 patients were of VD at neurological clinic and ward ofthe Second Affiliated Hospital of Chongqing Medical College,including 38 males and 26 females with theaverage age of(66.0±4.6)years(VD group).Culturallevel:19 cases re-ceiyed over senior school education,31 cases of junior school education and14 cases of illiteracy.Meanwhile 60 inpatients without VD were enrolled(non-VD group)including 35 males and 25 females withthe average age of(68.0±3.7)years.Culturallevel:senior school or above,15 cases,junior school, 29 cases and illiteracy,16 cases.METHODS:Mental sate of both VD and non-VD groups were assessed with mini-mental state examination(MMSE),and tested with P300 potential observing the variability of latency and amplitude of P300 in patients.Both methods were compared between VD group and non-VD group, moreover relationship between P300 latency and MMSE scores was also explored.MAIN OUTCOME MEASURES:Variability of P300 latency and amplitude Relationship between P300 latency and MMSE scores.RESULTS:P300 latency was found prolonged in 74% and 7% of VD group and non-VD group respectively,with obvious significance between them,meanwhile prolonged P300 latency was proved closely correlated with the MMSE scores(r=0.609 6,P＜0.05).CONCLUSION:P300 latency can be considered as an obiective predictor for the earlier diagnosis and the assessment of therapeutic effects in patients with VD.

Objective To assess the effectiveness of Chinese materia medica (CMM) as adjuvant therapy of antibacterial for pediatric mesenteric lymphadenitis.Methods Retrieved RCT research of CMM as adjuvant therapy of antibacterial for pediatric mesenteric lymphadenitis from CNKI,Wanfang,VIP,PubMed,and Medline database from 1980 to 2016.Literatures were selected according to the inclusion and exclusion crteria,Jadad scores method was used to evaluate the literatures and meta-analysis was performed by RevMan 5.3.Results A total of 34 randomized controlled trials were included.Meta-analysis showed that the trial group was superior to the control group in terms of efficiency,cure rate,remission time of main symptoms,mesenteric lymph node recovery,and recurrence rate,there was statistical difference.Conclusions CMM as adjuvant therapy of antibacterial for pediatric mesenteric lymphadenitis is more effective than the use of antibacterial alone.

At present,the study of intestinal absorption of oral drugs mainly includes in vitro,in vivo and in situ methods.In view of the advantages of in situ intestinal perfusion such as simple operation,mature technology,controllable,ensure the neuroendocrine regulation and blood supply,and so on,which could better reflect the true situation of drug absorption.In this study,the research methods and characteristics of intestinal absorption of oral drugs were systematically introduced.The recirculating perfusion method and single-pass perfusion method were compared,and several volume correction methods were also introduced.In order to ensure the operability and accuracy of experimental results,proper experiment method of intestinal absorption will be adopt according to the factors such as drug characters,experiment requirements,experimental conditions,and so on.The article provides a scientific basis for the development of pharmaceutical dosage and clinical rational drug use.

Liver cancer remains one of the leading cause of cancer death in the world.Animal models, especially mouse models, are important tools for studying the biological characteristics, pathogenesis, new drug screening and therapy of liver cancer.Up to now, although the development of various animal models accelerates the research of liver cancer, all the existing models have their own disadvantages.Lacking of economical and applicable animal models that can mimic the human liver cancer seriously restrict the further study of liver cancer.With the development of genetically modified technologies, it provides a fast, easy and reliable method to establish liver cancer models.In this review, we describe the different types of mouse models used in liver cancer research, with emphasis on genetically engineered mice used in this field, which may open an avenue for functional cancer genomics and generation of liver cancer models by using gene editing technologies.

Objective To analyze the application and funding for projects in radiation oncology from National Natural Science Foundation of China ( NNSFC ) from 2006 to 2015. Methods To collect the funding information in radiation oncology from NNSFC from 2006 to 2015, a computerized search was performed in the ISIS system using a subject code of H1610 and a keyword of radiation oncology. Analyses were performed in distribution of research fields, the geographical distribution of applicants, and the properties of institutes/universities the applicants were affiliated with. Results In the last decade, a total of 435 projects in the field of radiation oncology were funded with 180 million yuan. Most projects were funded by general, youth, and regional foundation, which covered the highest proportion of NNSFC. For a single project, the amounts of funding from general, youth, and regional foundations were 530, 220, and 400 thousand yuan, respectively. The institutes/universities the NNSFC?funded projects were affiliated with were located quite close to each other. The top 10 institutes/universities in terms of the number of NNSFC?funded projects covered 53% of projects. In all projects, 88% studied basic science, which covered many hot topics in oncology including biological effects of radiotherapy, microenvironment, and stem cells. A small number ( 12%) of projects focused on physics. Top 3 cancers in terms of the number of projects and the amount of funding were lung cancer, nasopharyngeal carcinoma, and esophagus cancer. Conclusions In the last decade, the field of radiation oncology has stable increases in the number of NNSFC?funded projects and the amount of funding. The NNSFC?funded research teams are unevenly distributed, most of which are located in East China. The most popular topic in basic science studies is about biological effects of radiotherapy.