Objective:To observe the effect of the combinative supplementation of Formoterol, Indomethacin and Roxithromycin on cancer cachexia (CC) and to study the mechanism. Methods: Male BALB/c mice bearing colon 26 adenocarcinoma for 9 days were served as models of cancer cachexia. The mice were divided into nine groups. Physiological conditions, body weight and food intake were documented every day. Serum levels of cytokine and nutritional markers were detected 7 days after treatment. Results: Tumor-bearing caused a wasting of non-tumor body weight and gastrocnemius muscle. Serum tumor necrosis factor-?, (TNF-?) and interleukin-6 (IL-6) levels were significantly elevated accompanied with nutrition depletion in tumor-bearing mice. After treatment with combinative supplementation of Formoterol, Indomethacin and Roxithromycin, left gastrocnemius weight and serum glucose were improved and the levels of TNF-? and triglyceride were down-regulated. Conclusion: Formoterol, Indomethacin and Roxithromycin had their own positive effects to CC mice. Drugs combinationin in tumor-bearing mice with cancer cachexia could produce synergistic action.[

Objective: To observe the effect of the combinative supplementation of Formoterol, Indomethacin and Roxithromycin on cancer cachexia (CC) and to study the mechanism. Methods: Male BALB/c mice bearing colon 26 adenocarcinoma for 9 days were served as models of cancer cachexia. The mice were divided into nine groups. Physiological conditions, body weight and food intake were documented every day. Serum levels of cytokine and nutritional markers were detected 7 days after treatment. Results: Tumor-bearing caused a wasting of non-tumor body weight and gastrocnemius muscle. Serum tumor necrosis factor-α, (TNF-α) and interleukin-6 (IL-6) levels were significantly elevated accompanied with nutrition depletion in tumor-bearing mice. After treatment with combinative supplementation of Formoterol, Indomethacin and Roxithromycin, left gastrocnemius weight and serum glucose were improved and the levels of TNF-α and triglyceride were down-regulated. Conclusion: Formoterol, Indomethacin and Roxithromycin had their own positive effects to CC mice. Drugs combinationin in tumor-bearing mice with cancer cachexia could produce synergistic action.

Objective To investigate the association of interleukin 1 receptor antagonist (IL 1Ra) gene polymorphism with osteoporosis in Chinese old men. Methods The allele frequency and the genotype distribution of the second intron of IL 1Ra gene in the groups of senile control (normal bone mineral density, n=65) and senile men with osteoporosis (n=68) were detected by polymerase chain reaction (PCR). Results The frequencyofA 2alleleincontrolgroup was higher than that in osteoporosis group (0.192 vs 0.103, P

Objective　To determine serum true insulin (TI) and proinsulin (PI) levels in patients with coronary heart disease (CHD), and to explore the relationships of them to other cardiovascular risk factors. Methods　Highly specific ELISA assays for TI and PI and RIA for immunoreactive insulin (IRI) were used. The fasting serum IRI, TI, PI levels in 30 nondiabetic patients with CHD and 30 healthy, age-matched control subjects were investigated. And the fasting and 2h postprandial plasma glucose, serum lipids and blood pressure were also determined in each individuals. Results　The levels of serum IRI, TI and PI were significantly higher in patients with CHD than those in controls (P＜0.05), while the value of insulin sensitivity index (ISI) was lower in patients with CHD than in controls (P＜0.05). There were significant independent correlations between TI, PI and age, BMI, plasma glucose, serum lipids. Conclusion Nondiabetic patients with CHD have hyperinsulinemia and insulin resistance and there is an independent association between proinsulin and dyslipidemia.

Objective To determine the causative organisms and antimicrobial susceptibility of community- and hospital-acquired pneumonia (CAP and HAP) in type 2 diabetes in Fujian Provincial Hospital. Methods The data of becteria spectrum and their drug susceptibility in patients with type 2 diabetes complicated by pulmonary infection were retrospectively analyzed in January 1995 to October 2006. Results The isolated bacteria of sputum culture of 494 cases included 73 gram-positive cocci(16.7%),139 gram negative bacilli(31.9%)and 224 fungus(51.4%).G+ cocci mainly included staphylococci,G-bacilli mainly included Klebsiella pneumoniae,Bowman immovability bacillus and Pseudomonas aeruginosa.In both CAP and HAP,fungus and G- bacilli were the dominant pathogens.The pathogenic bacteria were resistant to multi-antibiotics,and the resistant rates from HAP patients was higher than those from CAP. Of the bacterial strains isolated from blood culture,G-bacilli constituted 87.5%. Conclusions Fungus and G-bacilli were the dominant pathogens.Phlegm culture and drug sensitive test are helpful for reasonable use of antibiotics for patients with type 2 diabetes complicated by pulmonary infection in clinical practice.

The policy of“licensed doctors'multi-site clinical practice” aims to provide patients with a better access to quali-ty medical and health resources.Patient's understanding and support indicates the executive condition of the implementation of the pol-icy and it can also promote the implementation of the policy to a certain extant .In this study , the questionnaire survey and statistical tools were used to investigate the differences in their attitudes to the policy .

0.05).At the same time,loss of ranges of neck motion was 4.6??4.0?in group A and 11.6??7.8?in group B,significant difference was also seen(P

Objective To investigate the relation between paraoxonase (PON) 1 Q191R gene polymorphism and type 2 diabetes mellitus complicated with hypertension. Methods Genomic DNA was extracted from peripheral blood leukocytes. The studied DNA was amplified by PCR, and then digested with the restriction endonuclease AlwⅠ and separated on 2% agarose gel electrophoresis. PON activity was estimated by spectrophotometer. Serum insulin and C-peptide were measured by radioimmunoassay. Results The frequencies of QQ genotype and Q allele in type 2 diabetes mellitus with hypertension group were significantly higher than those in type 2 diabetes mellitus group (P

Objective To investigate the association of the polymorphism of endothelial nitric oxide synthase (eNOS) gene 27bp variable number of tandem repeats (VNTR) with essential hypertension (EH) and type 2 diabetes mellitus (DM) in Chinese population. Methods (1) Genotypes were determined by polymerase chain reaction (PCR)-agarose gel electrophoresis. (2) Fasting serum nitric oxide metabolites (NOx) were measured by nitrate reductase. (3) During oral glucose tolerance test (OGTT) serum immunoreactive insulin (IRI) and C peptide (CP) were measured by radioimmunoassay. Results (1) The frequenciesofaalleleinEHandtype2DMgroupwere significantly higher than that in control group (0.109 vs 0.051; 0.129vs 0.051, P

[Summary] Kisspeptin is vital for the neuroendocrine regulation of GnRH secretion. Kisspeptin neurons are now recognized as a central pathway responsible for conveying key homeostatic information to GnRH neurons. A number of metabolic modulators have been proposed as regulators of kisspeptin including leptin, adiponectin, insulin, ghrelin, POMC, and neuropeptide Y. Recent data indicate that kisspeptin may have a direct role in regulating energy balance by its regulation of food intake and glucose homeostasis. Thus, kisspeptin may serve as a new connection between reproductive function and energy metabolism.