Objective To evaluate the effect of isoflurane preconditioning on inflammatory responses during spinal cord injury (SCI ) in rats .Methods Sixty adult male Sprague-Dawley rats ,weighing 250-300 g , were randomly divided into 3 groups ( n= 20 each ) using a random number table :sham operation group (S group) , SCI group , and isoflurane preconditioning group (I group ) . The animals were anesthetized with intraperitoneal pentobarbital sodium 40 mg/kg .SCI was produced by a weight-drop contusion at the T10 level .The rats inhaled 2% isoflurane for 2 h ,and the model was established at 24 h after the end of isoflurane inhalation in I group . Neurological function was assessed and scored by using the the Basso , Beattie , Bresnahan (BBB ) Locomotor Rating Scale on 7 days after SCI .Five rats in each group were then chosen and spinal cord specimens were obtained and cut into sections which were stained with haematoxylin and eosin for determination of the viable neuron count .Fifteen rats in each group were sacrificed and the spinal cord was removed for detection of nuclear factor kappaB (NF-κB ) and interleukin-1β (IL-1β) expression (by Western blot ) .Results Compared with S group ,BBB score and the number of viable neurons were significantly decreased ,and the expression of NF-κB and IL-1βprotein was up-regulated in SCI group ( P<0.05) .Compared with SCI group ,BBB score and the number of viable neurons were significantly increased ,and the expression of NF-κB and IL-1βprotein was down-regulated in group I ( P<0.05 ) .Conclusion The mechanism by which isoflurane preconditioning protects the spinal cord is related to inhibition of inflammatory responses in rats .

Objective To explore the influencing factors of cervical cancer screening in women and to provide targeted health education so as to enhance the understanding of methods of cervical cancer and its screening tests among women. Methods Women with 21 to 70 years old in a community of Beijing were investigated on site with questionnaires to understand whether they carried out examination of cervical smear and its influencing factors. Results The average age of respondents was ( 40. 04 ± 13. 15) years old. Most people (78. 8%) knew the cervical cancer. Moreover, most of them (73.3%) had a proper knowledge that the cervical cancer could be prevented and cured. The number of people who knew the cervical smear was more than that who did not knew. Most of them knew on the examination via medical staff. People who had carried out cervical smear were in the minority. The results of χ2 test showed that there were statistically significant differences in the age (between the group of 21 to 29 years old and the rest), marital status, the profession (between the group of medical workers and the group without jobs) , monthly income ( between the group of 0 to 2 000 yuan and the rest);payment method for medical fee (between the group of self-paid medical fees and the rest), degree of knowledge on cervical cancer ( between the group in which people knew the cervical cancer and the rest ) . Conclusions The influencing factors of examination behavior of cervical smear include the age, marital status, profession, monthly income, payment method for medical fees, the knowledge of cervical cancer and cervical smear.