Objective:To investigate the expression of serum galectin-3 (Gal-3), cystatin-C (Cys-C) and endothelin-1 (ET-1) in elderly patients with hypertension and analyze their correlation with left ventricular hypertrophy(LVH).Methods:A total of over 65 years old 110 patients with essential hypertension in Tieling Central Hospital from July 2018 to May 2019 were selected as hypertension group, according to the LVH, patients with essential hypertension were divided into (non LVH group, 66 patients) and LVH group (44 patients). At the same time, a total of 110 healthy people in the same period were selected as control group. The expressions of serum Gal-3, Cys-C and ET-1 in each group were compared, and the correlation between left ventricular mass index (LVMI) and Gal-3, Cys-C in patients with essential hypertension were analyzed.Results:The levels of Gal-3, Cys-C and ET-3 in the hypertension group were higher than those in the control group: (132.28 ± 38.18) μg/L vs. (110.60 ± 17.51) μg/L, (1.19 ± 0.12) mg/L vs. (0.81 ± 0.06) mg/L, (21.28 ± 0.72) ng/L vs. (16.50 ± 0.68) ng/L, and the differences were statistically significant ( P<0.05). The levels of Gal-3, Cys-C and ET-1 in the LVH group were significantly higher than those in the non LVH group: (159.75 ± 36.37) μg/L vs. (113.96 ± 26.79) μg/L, (1.24 ±) mg/L vs.(1.16 ± 0.08) mg/L, (21.84 ± 0.71) ng/L vs.(20.90 ± 0.41) ng/L, and the differences were statistically significant ( P<0.05). Pearson correlation analysis showed that LVMI was positively correlated with Gal-3( r=0.650, P<0.01), Cys-C( r=0.659, P<0.01) and ET-1( r=0.858, P<0.01). The receiver operating characteristic(ROC) curve showed that the area under the curve (AUC) of Gal-3 in diagnosing hypertensive LVH was 0.821, 95% CI 0.743-0.899, the cutoff value was 148.55 μg/L, and the sensitivity and specificity were 59.10% and 89.40%. The AUC of Cys-C in diagnosing hypertensive LVH was 0.672, 95% CI 0.558-0.786, the cutoff value was 1.320 mg/L, and the sensitivity and specificity were 43.20% and 98.50%. The AUC of ET-1 in diagnosing hypertensive LVH was 0.876, 95% CI 0.811-0.941, the cutoff value was 22.275 ng/L, and the sensitivity and specificity were 65.90% and 95.50%. The AUC of combined detection was 0.948, 95% CI 0.855-0.976, and the sensitivity and specificity were 84.10%, 98.50%. Conclusions:The serum levels of Gal-3, Cys-C and ET-1 in elderly patients with hypertension are significantly increased, and there are a good correlation between them and LVH, and combined detection can be used as a sensitive index for the diagnosis of hypertensive LVH.

Objective To evaluate the clinical value of 64-slice spiral computed tomography combined with hs-CRP and MMP-9 to detect the vulnerable plaque of acute coronary syndrome.Methods Seventy-two patients with coronary heart disease were included and among them,43 patients were diagnosed as ACS and the other 29 patients diagnosed as stable angina group(SAP).All patients underwent 64-slice spiral computed tomography coronary artery imaging and examination of plasma hs-CRP and MMP-9 concentration.All patients underwent selective coronary angiography.Another 25 cases who underwent coronary angiography were included in the control group.Results The incidence of soft plaque was higher in the ACS group than in the SAP group(24 cases vs 4 cases,P=0.001).Compared with the control group,levels of hs-CRP and MMP-9 in the ACS group and the SAP group were both significantly higher(all P

OBJECTIVE: To observe the antitumor effect of FZQL combined with CTX on S180 tumor and the effect of FZQL on reducing CTX s toxieity .METHODS: The models of S180 tumor bering mice were established .The animals were divided into CTX therapy group, CTX and FZQL combined therapy group, FZQL therapy group and control group.The results were compared among these groups.RESULTS: The weight of tumor in FZQL therapy group was less than that in control group.All of the experimental markers in combined iherapy group were better than those in CTX therapy group.CONCLUSION: FZQL has a marked inhibiting effect on tumor(S180), and it can increase the CTX's inhibiting effect on tumor and reduce the side effect of CTX and increasing the body immunity.

DPN ( Diabetes Peripheral Neuropathy) is one of the most important complications of type 1 and type 2 diabetes, with high rate of mutilation, intractable character, and complicated etiological factors.. Therefore, it is necessary to replicate the animal model with typical characteristics of human DPN. This article reviewed the establishment of DPN animal model in recent years.

OBJECTIVE:To evaluate the efficacy and safety of salmon calcitonin plus Xianling gubao for osteoporosis and ostealgia in postmenopausal women. METHODS:160 women with postmenopausal osteoporosis and ostealgia were randomized to 3 groups (treatment group and 2 control groups):the treatment group received(500 IU q.d) salmon calcitonin nasal spray plus Xianling gubao(1.5 g b.i.d orally); the control group Ⅰ received(500 IU q.d) salmon calcitonin nasal spray alone and the control group Ⅱ received Xianling gubao(1.5 g b.i.d orally) alone. All the patients received oral Caltrate D (1 tablet q.d). A course was defined as 30 days. After treatment for a total of 3 courses,the nature and degree of ostealgia in all the patients were assessed,and the outcome indexes were measured and the adverse drug reactions were recorded. RESULTS:There were significant differences between the treatment group and two control groups in total effective rates(92.59% for treatment group vs. 69.81% for control group Ⅰ and 67.92% for control group Ⅱ,P0.05). CONCLUSION:Treatment of osteoporosis and ostealgia in postmenopausal women with salmon calcitonin plus Xianling gubao has been proved to be safe and effective in that the bone density can be increased effectively and the osteoporotic pain of lumbar and back of the patients can be relieved.

Objective The different distribution and clinical significance of mean platelet volume (MPV) in the healthy normoglycemic and impaired fasting glucose (IFG) individuals were discussed.Methods The 499 individuals including 184 male and 315 female,who had undergone health checks in Tianjin Huanhu Hospital during May and July 2012 were studied retrospectively.Average age is forty-five ( thirty-five to eighty).Subjects were categorized into four groups according to fasting plasma glucose ( FPG) levels:G1 (3.89 mmol/L≤FPG<5 mmol/L, n=125),G2(5 mmol/L≤FPG <5.5 mmol/L, n=121), G3(5.5 mmol/L≤FPG<6.1 mmol/L, n=142), and G4(6.1 mmol/L≤FPG<7 mmol/L, n=111).G1, G2, and G3 are defined as normal FPG groups and G 4 is defined as IFG group.Eighty-nine cases in the same age patients with type II diabetes mellitus group ( G5 ) were observed at the same time.Results The MPV increased with the increasing FPG levels in the following order:G1(8.62 ±0.77) fl, G2 (8.85 ±0.80) fl, G3(8.90 ±0.69) fl,G4(9.14 ±0.78) fl and G5(12.03 ±1.42) fl.MPV[(12.03 ±1.42) fl] of type Ⅱdiabetes mellitus group(G5) was higher than that in the IFG group (G4)[(9.14 ±0.78) fl] and normal FPG groups[G1(8.62 ±0.77) fl,G2(8.85 ±0.80) fl,G3(8.90 ±0.69) fl] (F=12.773,P<0.01);MPV of the IFG group [ ( 9.14 ±0.78 ) fl ] was significantly higher than that in normal FPG groups [ G1 (8.62 ±0.77) fl,G2(8.85 ±0.80) fl,G3(8.90 ±0.69) fl] (F=12.773,P<0.01 for G4 vs.G1 and G2, P<0.05 for G4 vs.G3) ;MPV in the high-normal glucose group (G3) [(8.90 ±0.69) fl] was obviously higher than that in the low-normal glucose group (G1) [(8.62 ±0.77) fl] (F=12.773,P<0.05);MPV was positively associated with FPG in normal FPG groups ,IFG group and type Ⅱ diabetes mellitus group (G1-3:r=0.22, P<0.05;G4:r=0.26, P<0.01;G5:r=0.29, P<0.01).Conclusions Significant difference of MPV was observed in population of different FPG levels.Especially, MPV in IFG group was evidently higher than that in normal FPG group and was positively associated with FPG levels.

Objective To explore the role of Th17 cells in systemic lupus erythematosus (SLE)with cardiovascular disease (CVD).Methods 61 patients of SLE were collected from September 2011 to March 2013 in the Second Hospital of Hebei Medical University by revised SLE classification standards of ACR in 1997.These patients were divided two groups:22patients of SLE with CVD and 39 patients of SLE without CVD;the control group include 20 healthy.Th17 cells were measured by flow cytometry,IL-1 7 was detected by enzyme-linked immunosorbent assay.The correlation among them and the disease active index were analyzed.Results ①The percent of Th1 7 cells in the group of SLE with CVD,that in the group of SLE without CVD and that in control group were (2.09±0.98)%,(1.75±0.75)% and (0.89±0.44)%,respec-tively.The percent of Th1 7 cells in healthy group were lower than that in SLE with CVD and SLE without CVD group (t=4.717~5.030,P<0.001).The level of IL-17 in the group of SLE with CVD,that in the group of SLE without CVD and that in control group were 85.64±20.76 pg/ml,75.25±28.14 pg/ml and 35.06±6.58 pg/ml respectively,and the serum of IL-17 in healthy group were lower than in SLE with CVD and SLE without CVD group (t=6.275~9.954,P<0.001). There were no significant difference of Th1 7 cells and IL-1 7 between SLE with CVD and SLE without CVD groups (t=1.520,P>0.05;t=1.513,P>0.05).②The level of IL-17 were correlated positively with SLEDAI and the anti-double strand DNA (r=0.393,P=0.008;r=0.558,P<0.001),were correlated negatively with complement C3 (r=-0.423,P=0.005).The percent of Th17 cells in CD4+T cells were correlated positively with SLEDAIand the anti-double strand DNA (r=0.681,P<0.001;r=0.492,P=0.015)were correlated negatively with complement C3 (r=-0.534,P=0.027).Con-clusion The level of Th1 7 cells and IL-1 7 were high in SLE,and they were related with the disease activity.The cardiovas-cular factor had not affect the expression of Th1 7 cells and IL-1 7 in SLE.

OBJECTIVE: To compare inhibition effects of arsenacetylic acid(ASAC) on experimental H22 hepatoma-bearing mice in different forms of administration. METHODS: The mice were divided into 5 groups at random after inoculated with H22 hepatoma into their right axillas hypodermic by intraperitoneal injection(ip) and intravenous injection(iv), and then injected with normal saline,cyclophosphamide and different dosage of ASAC, observe the rate of tumor strain becoming tumor, the inhibition effects of the subjects and the effects of the subjects on mice's viscera. RESULTS: Compared with ip,either high, moderate or low dosage of ASAC by iv did have an obvious inhibitory effect on tumor and the tumor inhibition rates were 46.59%, 46.31% and 32.48% respectively; however, the spleen index in groups that of lower dosage of ASAC by two forms of drug administration were increased; there were death by poisoning in the group that iv with high dosage of ASAC.CONCLUSION: Compared with ip, the administration of ASAC by iv has a better effect on tumor.

Glaucoma is an irreversible cause of blindness,and it does serious harm to visual functions.Symptoms of glaucoma,visual impairment,a variety of symptomatic treatment and adverse reactions of drugs result in great obstacles and reduce quality of life.Preventive treatment intervenes glaucoma at an early stage by providing patients with guidance on therapies,diet and daily life,which are effective and may effectively improve their quality of life.

AIM:To investigate the effect of Sonic Hedgehog ( Shh) signaling blockade on the growth of hema-tocarcinoma cells and underlying mechanisms.METHODS: The expression of Shh signaling molecules in hematocarci-noma cell lines BEL-7402, Huh7 and HepG2 was detected by RT-PCR.The cell viability was detected by MTT assay.The cell cycle and apoptosis were analyzed by flow cytometry.The expression of apoptosis-related proteins was determined by Western blot.RESULTS:Shh signaling molecules were all expressed in BEL-7402, Huh7 and HepG2 cells.The mRNA expression of Patched ( Ptch) , Gli1 and Gli2 was down-regulated by anti-Shh antibody.Blockade of Shh signaling pathway inhibited the proliferation of hepatocarcinoma cells with increasing cells in G0/G1 phase and induced the apoptosis of hepa-tocarcinoma cells.Treatment with anti-Shh antibody down-regulated the protein expression of pro-caspase-3, pro-caspase-8 and pro-caspase-9, while up-regulated the protein levels of cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 in BEL-7402 cells.CONCLUSION:Blockade of Shh signaling pathway inhibits the growth of hepatocarcinoma at different levels by cell cycle arrest and inducing apoptosis of hematocarcinoma cells.