OBJECTIVE To compare the monitoring effect of 2 kinds of B-D testing package.METHODS 2 kinds of testing package around the air vent of first pot sterilizer before antiseptic every day were used preheating 5 min.Start to move the B-D procedure when the band pressure to achieve above 100 kPa with 3 time of pulsation vacuum,inner chamber pressure of 210 kPa,the temperature of 134 ℃ and time of 3.5 min.RESULTS Except the false negative caused by package factor,the testing accurate rate of disposable B-D testing package was 100%.But the testing accurate rate of repeated uses B-D testing package was 90%.CONCLUSIONS The disposable B-D test package may respond promptly the breakdown of sterilizer and avoid the false negative result which the artificial pack factor creates.The test result is accurate.The disposable B-D testing package for examination of autoclave exhaust cold air degree is obviously better than the self-made cotton fabric testing package.

Six nucleosides and base components in Four products of Mycelia of cultivated Cordyceps were determined. The comparison of extraction solvents and recovery test of the adenosine components were emphasized. The effective extraction-rate by water,as an extraction solvent, was higher than that by 50% alcohol. The addition of 0. 1% sodium azide into water could not only preserve putrefaction,but also make a contribution to the stability of nucleoside components in the liquid sample. When the extraction was carried out by ultrasonic at room temperature,the recovery rate of the added sample was 99. 8%.

Objective To explore the curative effect and safety of of Entecavir ( ETV) combined Liuweiwul-ing Pian in the treatment of chronic hepatitis B patients with HBeAg +.Methods 124 patients according to the ran-dom number table were divided into the two groups ,which respectively used ETV combined Liuweiwuling Pian treat-ment and ETV.The liver function and index of viral replication of the two groups were compared .Results The effec-tiveness of the observation group was 91.94%,which of the control group was 82.26%,the difference between the two groups had statistical significance(Ridit=32.251,P=0.000);Since 24 week treatment,HBV DNA of the observa-tion group was lower than the control group,the difference was statistically significant (t =2.365,4.595,all P<0.05);After treatment,ALT,AST of the observation group decreasing degree was higher than the control group ,the difference was statistically significant (t=78.512,62.145,all P<0.05);after treatment,the ALT rate,effective rate and bounce rate in the two groups was statistically significant differences (χ2 =25.621,46.241,11.512, all P<0.05);After treatment,the HBV DNA and HBeAg positive in two groups was below cut-off test cases,differences was statistically significant(χ2 =17.265,21.264,all P<0.05).Conclusion Entecavir combined Liuweiwuling Pian in the treatment of chronic hepatitis B patients with HBeAg +can reduce liver fibrosis , and has obvious detoxification and anti-viral.

[Objective] To investigate the effect of electroacupuncture (EA) of Neiguan (PC6) poinl on rnyocardial fibrosis and myocardial contents of collagen type Ⅰ and type Ⅲ in rats with pressure overload. [ Methods ] Rats were randomized to four groups: pseudo-operation group ( group A), model group ( group B) , EA group ( group C) and western medicine group (group D). Pressure-overloaded rat models were established by restriction of abdominal aortic artery. Left ventricular mass index (LVMI) and blood pressure (BP) in the four groups were observed; the plasma level of angiotensin Ⅱ (Ang Ⅱ ) was detected by radioimmunoassay and myocardial contents of collagen type Ⅰ and type Ⅲ by immunohistochemical staining method. [Results] BP, LVMI, expression levels of Ang If and collagen type Ⅰ and type Ⅲ were increased in group B as compared with those in group A ( P 0.05) . [Conclusion] EA of Neiguan point is effective in preventing and treating myocardial fibrosis induced by pressure overload.

Legionnaires’disease is an atypical pneumonic illness caused by the legionella bacteria.These bacteria cause respiratory diseases when a susceptible host inhales aerosolized water containing the bacteria or aspirates water containing the bacteria.Legionella are ubiquitous in natural and artificial water environments worldwide,and survive in a range of environmental conditions.Cooling towers have been implicated in many outbreaks of legionellosis.The causative organism has been readily isolated from many of cooling towers.Various physical and chemical disinfection methods have been developed to eliminate legionella from cooling waters,but gaining control of legionella in cooling water remains a challenge,more methods should be found to control legionella in cooling water.The related researches were reviewed in this paper.

Object To explore the difference of explicit and implicit depression stereotypes for people with major depressive disorder, social public, psychiatrists and nurses.Methods 61 major depressive disorder patients(MDD) ,55 social public people(SPP) and 56 psychiatrists and nurses(PN) were enrolled.All participants were measured by explicit depression stereotype evaluations and implicit association tests.Results There was significantly difference on the positive explicit stereotype scores about depressive stereotypes (DS) among the MDD (2.31±1.60) ,SPP(2.43±1.55) and PN(3.12±1.58)(F=23.22, P=0.00).LSD test showed significantly difference on the positive explicit stereotype scores about DS between the MDD and PN,with the similar to the SPP and the PN(P＜0.05).There was significantly difference on the negative explicit stereotype scores about DS among the MDD(4.09±2.22), SPP (3.97±2.01) and PN (3.23± 1.64) (F=30.16, P=0.00).LSD test showed significantly difference on the negative explicit stereotype scores about DS between the MDD and PN,with the similar to the SPP and the PN (P＜ 0.05).There was no significantly difference on the implicit stereotype scores about DS among the MDD,SPP and PN(F=2.03, P=0.12).Conclusion The MDD,SPP and PN show negative impicit stereotype about DS.However,the PN shows positive explicit stereotype about DS.

Objective To investigate the clinical significance of the changes of hypersensitive C-reactive protein (hs-CRP) and myocardial enzyme spectrum,cardiac troponin Ⅰ (cTn Ⅰ) in patients with Henoch-Schnlein purpura.Methods 100 patients with Henoch-Schnlein purpura were selected.50 patients only skin involved were selected as the simple group,50 patients accompanied by other organs involved were selected as mixed group.50 healthy people in the same period were selected as the control group.The serum hs-CRP,myocardial enzyme spectrum and cTn Ⅰ were tested and compared.Results The hs-CRP,serum myocardial enzyme spectrum and cTn Ⅰ levels of mixed and simple group were significantly higher than the control group(all P ＜ 0.05) ; Serum CK-MB,cTn Ⅰ and hs-CRP levels of the mixed group were significantly higher than those of simple group (all P ＜ 0.05).The abnormalities number of CK-MB and cTn Ⅰ respectively accounted for 33％ (33/100) and 32％ (32/100) ;The constituent ratio of the number of abnormal serum CK-MB and cTn Ⅰ of the mixed group was significantly higher than the simple group (x2 =4.047,3.908,all P ＜ 0.05) ;The Pearson correlation analysis showed that the relationship between the serum hs-CRP and CK-MB,cTn Ⅰ levels was linear,which were positively correlated (r =0.872,0.801,all P ＜ 0.05).Conclusion Patients with allergic purpura should early detect the serum levels of hs-CRP,myocardial enzyme spectrum and cTn Ⅰ,and treat the myocardial injury in time.

BACKGROUND:Modified glass ionomer cements improve the mechanical properties, abrasion resistance and beauty of traditional glass ionomer cements, and therefore they have been gradualy adopted in many fields of oral clinical application. However, it is not clear that the fluoride release behavior and soluble characteristics. OBJECTIVE:To investigate the fluoride release behavior and soluble characteristics of four glass ionomer cements in artificial saliva: Fuji II tranditional type, Fuji Plus hydroxyapatite modified type, Fuji IX metal modified type, and Fuji II LC light-curing resin modified type. METHODS: For each glass ionomer cement, cylindrical specimens were made and immerged into the artificial saliva. Fluoride release within 0-28 days was measured by using fluoride ion selective electrode. The weight of specimens was measured by using an analytical balance to further calculate the solubility. SPSS and Origin software were used for statistical analysis and nonlinear regression analysis, respectively. RESULTS AND CONCLUSION:For each glass ionomer cement, the fluoride release was peaked at the first day, decreased rapidly for the folowing several days, and then kept relatively stable at the end. The daily amount and accumulation of fluoride of Fuji II LC were significantly higher than those of other three glass ionomer cements (P < 0.01). The formula (Y=a + b* t0.5 + c* t) could better simulate the variation of fluoride release accumulations, and the fluoride release accumulations were in approximate proportional relationship with solubility. Fuji II LC reached the maximum fluoride release accumulations and the maximum ratio of fluoride release accumulations to solubility.

BACKGROUND:Children teeth filed with glass ionomer cement are stil susceptible with secondary caries, which is in close relationship with complex microbial community in dental plaque on the surface of glass ionomer cement. Traditional microbial methods are incapable of getting important information towards dental plaque microbes. OBJECTIVE:To analyze microbial community structure and numerical level of caries-induced microbes in dental plaque on the surface of glass ionomer cement for different caries-susceptible children. METHODS:Twenty-four children (age: 3-5 years) were divided into the caries-free, caries-positive, and caries-active children groups by the decayed, missing and filed index. With eight individuals in each group, their dental plaques were sampled for microbial community analysis. Denaturing gradient gel electrophoresis was employed to make clear the microbial community diversity and species identity in dental plaque of the caries-free, caries-positive, and caries-active children groups. Fluorescentin situ hybridization was used to investigate the numerical level of the caries-induced microbeStreptococcus spp. Quantitative PCR was carried out to analyze relative quantity ofStreptococcusmutans in total bacteria. RESULTS AND CONCLUSION:Compared with the caries-positive and caries-active children groups, microbial community diversity among samples was significantly higher in the caries-free group. Microbes abound in the caries-positive and caries-active groups might act important roles in the development of caries.Streptococcus spp. and Actinomyces spp. might be important caries-induced microbes in the caries-active group. The ratios ofStreptococcus spp. andStreptococcus mutans in total bacteria were significantly higher in the caries-free group than those in the caries-free and caries-positive groups. In summary, molecular ecology technologies can wel reflect caries-related complex microbial community in dental plaque.

BACKGROUND:Glass ionomer cements have been gradual y employed in many aspects of dental clinical field. However, low mechanical strength and antibacterial effect restrict its further applications. OBJECTIVE:To proportional y explore the effects of titanium dioxide nanoparticles on the mechanical strength and antibacterial effect of traditional glass ionomer cements. METHODS:Base on different mass fractions of titanium dioxide nanoparticles in glass ionomer cements, al the glass ionomer cement samples were divided into control group (no titanium dioxide nanoparticles), low titanium dioxide group (containing 3%titanium dioxide nanoparticles), medium titanium dioxide group (containing 6%titanium dioxide nanoparticles), and high titanium dioxide group (containing 9%titanium dioxide nanoparticles). A universal material testing machine and a hardness tester were used to examine flexural strength, compressive strength, and surface hardness of glass ionomer cement samples, respectively. Glass ionomer cement samples were immerged into the artificial saliva, and fluoride release was measured by using a fluoride ion selective electrode. The direct contact test was used to investigate antibacterial effect of glass ionomer cement samples towards Streptococcus mutans. RESULTS AND CONCLUSION:Compared with the control group, few titanium dioxide nanoparticles (low and medium titanium dioxide group) could significantly improve flexural strength, compressive strength and surface hardness of glass ionomer cement samples (P<0.05), and high titanium dioxide nanoparticles (high titanium dioxide group) significantly decreased flexural strength, compressive strength and surface hardness (P<0.05). The introduction of titanium dioxide nanoparticles had little effect on fluorine release behavior of glass ionomer cement samples, and greatly improved antibacterial effect of glass ionomer cement samples towards Streptococcus mutans.