BACKGROUND:Children teeth filed with glass ionomer cement are stil susceptible with secondary caries, which is in close relationship with complex microbial community in dental plaque on the surface of glass ionomer cement. Traditional microbial methods are incapable of getting important information towards dental plaque microbes. OBJECTIVE:To analyze microbial community structure and numerical level of caries-induced microbes in dental plaque on the surface of glass ionomer cement for different caries-susceptible children. METHODS:Twenty-four children (age: 3-5 years) were divided into the caries-free, caries-positive, and caries-active children groups by the decayed, missing and filed index. With eight individuals in each group, their dental plaques were sampled for microbial community analysis. Denaturing gradient gel electrophoresis was employed to make clear the microbial community diversity and species identity in dental plaque of the caries-free, caries-positive, and caries-active children groups. Fluorescentin situ hybridization was used to investigate the numerical level of the caries-induced microbeStreptococcus spp. Quantitative PCR was carried out to analyze relative quantity ofStreptococcusmutans in total bacteria. RESULTS AND CONCLUSION:Compared with the caries-positive and caries-active children groups, microbial community diversity among samples was significantly higher in the caries-free group. Microbes abound in the caries-positive and caries-active groups might act important roles in the development of caries.Streptococcus spp. and Actinomyces spp. might be important caries-induced microbes in the caries-active group. The ratios ofStreptococcus spp. andStreptococcus mutans in total bacteria were significantly higher in the caries-free group than those in the caries-free and caries-positive groups. In summary, molecular ecology technologies can wel reflect caries-related complex microbial community in dental plaque.

BACKGROUND:Glass ionomer cements have been gradual y employed in many aspects of dental clinical field. However, low mechanical strength and antibacterial effect restrict its further applications. OBJECTIVE:To proportional y explore the effects of titanium dioxide nanoparticles on the mechanical strength and antibacterial effect of traditional glass ionomer cements. METHODS:Base on different mass fractions of titanium dioxide nanoparticles in glass ionomer cements, al the glass ionomer cement samples were divided into control group (no titanium dioxide nanoparticles), low titanium dioxide group (containing 3%titanium dioxide nanoparticles), medium titanium dioxide group (containing 6%titanium dioxide nanoparticles), and high titanium dioxide group (containing 9%titanium dioxide nanoparticles). A universal material testing machine and a hardness tester were used to examine flexural strength, compressive strength, and surface hardness of glass ionomer cement samples, respectively. Glass ionomer cement samples were immerged into the artificial saliva, and fluoride release was measured by using a fluoride ion selective electrode. The direct contact test was used to investigate antibacterial effect of glass ionomer cement samples towards Streptococcus mutans. RESULTS AND CONCLUSION:Compared with the control group, few titanium dioxide nanoparticles (low and medium titanium dioxide group) could significantly improve flexural strength, compressive strength and surface hardness of glass ionomer cement samples (P<0.05), and high titanium dioxide nanoparticles (high titanium dioxide group) significantly decreased flexural strength, compressive strength and surface hardness (P<0.05). The introduction of titanium dioxide nanoparticles had little effect on fluorine release behavior of glass ionomer cement samples, and greatly improved antibacterial effect of glass ionomer cement samples towards Streptococcus mutans.

BACKGROUND:Modified glass ionomer cements improve the mechanical properties, abrasion resistance and beauty of traditional glass ionomer cements, and therefore they have been gradualy adopted in many fields of oral clinical application. However, it is not clear that the fluoride release behavior and soluble characteristics. OBJECTIVE:To investigate the fluoride release behavior and soluble characteristics of four glass ionomer cements in artificial saliva: Fuji II tranditional type, Fuji Plus hydroxyapatite modified type, Fuji IX metal modified type, and Fuji II LC light-curing resin modified type. METHODS: For each glass ionomer cement, cylindrical specimens were made and immerged into the artificial saliva. Fluoride release within 0-28 days was measured by using fluoride ion selective electrode. The weight of specimens was measured by using an analytical balance to further calculate the solubility. SPSS and Origin software were used for statistical analysis and nonlinear regression analysis, respectively. RESULTS AND CONCLUSION:For each glass ionomer cement, the fluoride release was peaked at the first day, decreased rapidly for the folowing several days, and then kept relatively stable at the end. The daily amount and accumulation of fluoride of Fuji II LC were significantly higher than those of other three glass ionomer cements (P < 0.01). The formula (Y=a + b* t0.5 + c* t) could better simulate the variation of fluoride release accumulations, and the fluoride release accumulations were in approximate proportional relationship with solubility. Fuji II LC reached the maximum fluoride release accumulations and the maximum ratio of fluoride release accumulations to solubility.

Background Herpes simplex virus (HSV) disciform stromal keratitis is a T cell-mediated delayed-type hypersensitivity of corneal stroma.The treatment of HSV disciform stromal keratitis is the combination of glucocorticoid and antiviral drug before.However,the therapy is limited for intolerantble patients to glucocorticoid.In addition,the adverse reactions following usage of glucocorticoid can not be ignored.FKS06 solution is proved to has the immunosuppressive and antiinflammatory acttions with less side effects than glucocorticoid drug.But the study on the application of FK506 solution in treatment of HSV disciform stromal keratitis is lack.Objective This prospective clinical trail was to evaluate the efficacy of topical tacrolimus for HSV disciform stromal keratitis and its safety.Methods A prospective study was performed on 18 consecutive cases (18 eyes) who were diagnosed as active HSV disciform stromal keratitis from June 2011 to June 2012 in Zhongshan Ophthalmic Center under the approval of the Ethic Committee of Zhongshan Ophthalmic Center and informed consent of the patients,including 3 patients with refractory to prior steroid eyedrops.All patients received a combination topical tacrolimus eyedrops 4 times per day with topical and systemic anti-viral therapy for mean (56.0±7.9) days.Corneal thickness by slit lamp biomicroscope,visual acuity,intraocular pressure,corneal fluorescein staining,Schirmer Ⅰ test (S Ⅰ t) and tear breakup time (BUT) were performed on the patients before and 1 month,2 months after treatment and at the drug withdrawal to assess the treating efficacy of FK506,and the adverse reactions of eyes were recorded.Results Corneal edema was alleviated in (8.3 ± 2.5) days after treatment in all the patients and returned to normality in (25.3±11.5) days.Corneal edema of 2 eyes with refractory to previous steroid eyedrops showed a slower recovery procedure than that of the others.Corneal fluorescence staining scores were 4 (6,2),1 (2,1),1 (1,0) and 0 (1,0) before and 1 month,2 months and at drug withdrawal,respectively,with a significant difference among the four time points (H=39.90,P＜0.001),and the scores were significantly lower after treatment than before (all at P＜0.01).The S Ⅰ t values were (6.78±1.90),(7.39±3.53),(8.06±2.92) and (8.11±3.05) mm/5 min,respectively in the 4 time points,without statistically significant difference among various time points (F =0.94,P =0.43).The BUT values were (0.39 ± 0.50),(1.11 ± 0.90),(2.00 ± 1.08) and (3.39 ± 0.92) seconds,respectively,showing a significant difference among the 4 time points (F=34.54,P＜0.01) and had the increased values after treatment of FK506.Recurrence was seen in 2 eyes in 2 months and 5 months after cessation of tacrolimus.However,the eyes were healed after a second round regimen.No elevation of intraocular pressure and secondary infection were found in the patients during the treatment and follow-up period.Conclusions The combination therapy of tacrolimus with antiviral drug is effective and safe for HSV disciform stromal keratitis by alleviating corneal edema and promoting the rehabilitation of tear film.

Objective To compare the antalgic effects of ropivacaine in various doses on brachial plexus nerves. Methods 87 patients undergoing upper arm surgery were enrolled in this study. The patients were unpremed-itated. The patients were randomized to receive ropivacaine of 0.30,0.25 or 0.20% (group Ⅰ,Ⅱ and Ⅲ) with mor-phine of 0.1mg/ml and Lidocaine of 10mg/ml. In order to analygesia of brachial plexus nerves,the rejecting of load dose is firstly 10ml,the total dose is 10ml/24h interval every time,the antalgic time is about 50 hours. To compare the antalgic effects of ropivacaine in various doses based on standard of VAS. Based on standard of Bromage to assess the situation of motor neuron blocking. Results According to standard of VAS in 6,12,24 and 48 hours after underwent surgery, the group Ⅲ is more effective than group Ⅰ and group Ⅱ ; according to standard of Bromage, the group Ⅰ is more effective than group Ⅱ and group Ⅲ. it has no adverse reactions to patients. Condusion 0. 25% of ropiva-eaine with morphine of 0.1mg/ml and lidocaine of 10mg/ml have obtained good antalgic effects for patients after un-dergoing upper arm surgery,it is fit for applying in clinic.

OBJECTIVETo investigate the influence of parathyroid hormone and estrogen on alveolar bone metabolism of castrated female rats.

METHODSSixty-six female Wistar rats which were healthy and 4 months old were divided into two groups, with group SHAM (n = 18) and group ovariectomy (OVX) (n = 48). After 8 weeks of ovariectomy, the osteoporosis model was confirmed by examing 8 ovariectomized and sham-operated rats. The rest 10 rats in group SHAM were the control group (group A). The rest 40 rats in group OVX were divided into ovariectomized group (group B), ovariectomized and treated with estrogen (group C), ovariectomized and treated with parathyroid hormone (group D), ovariectomized and treated with estrogen and parathyroid hormone (group E) at random with 10 in each group. Group A and B injected physiological saline (1 mL x kg(-1)), group C injected estradiol benzoate (10 microg x kg(-1)), group D injected parathyroid hormone (20 microg x kg(-1)), group E injected parathyroid hormone (20 microg x kg(-1)) and estradiol benzoate (10 microg x kg(-1)). The intraperitoneal injection were maken every other day to rats in each group, which continued for 8 weeks. The bone mineral density (BMD), bone histomorphology and serum Ca, P, alkaline phosphatase (ALP) were measured after therapy.

RESULTSAfter 8 weeks of ovariectomy, the lumbar BMD of ovariectomized rats were significantly declined compared with those of the sham-operated rats (P < 0.05). Eight weeks later after the drug use, the BMD, %Tb.Ar, Tb.Th, Tb.N in group C, D, E were slightly elevated compared to group B, especially the group E (P < 0.05). Serum calcium and phosphorus values did not change significantly (P > 0.05). ALP values in group B was significantly higher than that in group A (P < 0.05).

CONCLUSIONIntermittent application of parathyroid hormone in small doses can increase alveolar BMD of castration rats and improve their bone structure. And it can have synergy effects on the treatment of osteoporosis if it is used combining with estrogen.

Alkaline Phosphatase ; Animals ; Bone Density ; Estradiol ; analogs & derivatives ; Estrogens ; Female ; Osteoporosis ; Ovariectomy ; Parathyroid Hormone ; Rats ; Rats, Sprague-Dawley ; Rats, Wistar

Biomedical Research ; trends ; Female ; Humans ; Hydatidiform Mole ; diagnosis ; genetics ; Pregnancy ; Uterine Neoplasms ; diagnosis

Objective To study the relationship between the mutation of the inverted repeat (IR) gene in the multiple transferable resistant (mtr) system and multiple antibiotic resistance of Neisseria gonorrhoeae. Methods The antimicrobial susceptibilities of isolated strains were tested. An agar plate dilution method was used to determine the minimum inhibitory concentrations. The target genes were amplified by PCR and subjected to sequencing. Results No mutation was found in the IR gene of either of 2 sensitive or 5 penicillin-resistant Neisseria gonorrhoeas strains. Among the 17 multiple-antibiotic-resistant strains, a strain with both azithromycin- and penicillin-resistance had T/A and T/A insertions, and another had A/T deletion. Conclusion Mutations in the IR gene of the mtr system of Neisseria gonorrhoeae might result in multiple antibiotic resistance.

Objective To explore the association between clusterin(CLU) gene rs11136000 poly-morphism and late-onset Alzheimer's disease ( LOAD) in Bai population from Dali Bai Autonomous Prefec-ture of Yunnan Province.Methods A case-control study including 109 LOAD patients and 120 normal con-trols matched for age,sex and level of education was taken in Dali Bai population.Polymerase chain reaction (PCR) and single nucleotide polymorphism (SNP) site testing were used to detect genotype and allele fre-quency of CLU SNP rs11136000.SPSS 17.0 was applied to analyze the data.Result ①The different fre-quency ( C:65.60%,T:34.40%,CC:38.53%,CT:54.13%,TT:7.34%) of CLU SNP rs11136000 genotypes and alleles distribution in Bai between LOAD patients and healthy controls showed no statistical significance (χ2=1.529, P=0.216;χ2=2.805, P=0.246) .②The serum total cholesterol ( TC) of LOAD patients was significantly higher than that in that of control group( t=2.508, P=0.013) .Conclusion The results suggest that CLU rs11136000 polymorphism may not be the susceptible gene of LOAD,and high serum total choles-terol is more common in LOAD patients.

Objective To determine whether the recorabinant murine interleukin-6 antisense RNA (IL-6 RNAAS) can regulate the proliferation of murine glomerular mesangial cell (MC) and the expression of protein and IL-6 mRNA that derived from the MC. Methods IL-6 RNAAS was led into the package cell PA31; by lipofect AMINE. Presence of IL-6 RNAAS in the supernatant of the cultural PA3n cells was assured by G4u screening and PCR check. The MC proliferation, IL-6 activity and IL-6 mRNA expressionin of MC were detected by 3 H-thymidine, KD3,7 cell line and Northern blot respectively. Results The pse.udovirus liter in the PA317 cell supernatant reached 1 ?107 CFU/ml. The IL-6 RNAAS could incorporate into MC and was expressed. The 3H-thymidine incorporation, IL-6 mRNA expression and MC-derived IL-6 were significantly lower than that in control group. Conclusion IL-6 RNAAScan inhibit MC proliferation and regulate downward the secretion and expression of IL-6 in MC.