Objective To study the effects of aerobic exercises on platelet function in patients with phase Ⅰ essential hypertension (EH). Methods Low-intensity aerobic exercises therapy was given to 36 patients with phase Ⅰ EH for three months. Changes of their blood pressure, heart rate, platelet aggregation rate (PAGTmax), alpha-granule membrane protein (GMP-140) and mean platelet volume (MPV) werer ecorded and compared to those in 36 normal healthy controls. Results As compared to those in controls,PAGTmax, level of GMP-140 and MPV were all increased in patients with phase Ⅰ EH before exercises therapy(t=15.221, 10. 916 and 16. 401, respectively, all P < 0. 01 ). After exercises therapy, 24-hour mean blood pressure decreased in association with reduction in PAGTmax to (0. 80±0. 05)% from (0. 88±0. 08) % (t=4. 323, P < 0. 05), level of GMP-140 decreased to ( 28±20) μg/L from (57±23 ) μg/L ( t=15.049, P<0.01) and MPV decreased to (9.0±0.9)fl from (9.1±0.9)fl (t=5.518, P <0.05) before it. But, there was no significant difference in these in dicators of platelet function in controls before and after exercise therapy (P>0.05). Conclusions Aerobic exercises therapy could be effective in lowering blood pressure in patients of mild EH with increased platelet activation, as well as inhibition ontheir platelet activation.

Objective To investigate the change of heart rate variability(HRV) in patients with white coat hypertension (WCH).Methods Participants were classified as essential hypertension (EH) group (n=35),white coat hypertension (WCH) group (n=35),normotension (NT) group (n=35) based on clinic and 24 h ambulatory blood pressure monitoring.The time and frequency-domain measures of HRV were measured by 24 h ambulatory electrocardiographic recording.Results Compared with NT controls,the standard deviation of normal-to-normal intervals (SDNN),rate-mean-square of the difference of successive RR intervals (RMSSD) and high frequence (HF) in the patients with EH and WCH were significantly decreased[SDNN:NT(132.2?21.5)vs EH(115.9?18.9)vs WCH(120.6?17.5)ms;RMSSD:NT(36.8?9.3)vs EH(28.6?8.9)vs WCH(27.5?8.5)ms;HF:NT(206.8?99.7)vs EH(159.5?86.6)vs WCH(161.1?84.5)ms2/Hz;all P0.05).Conclusion These changes in the parameters of HRV showed cardiac autonomic nerve dysfunction in white coat hypertensive patients.

Six nucleosides and base components in Four products of Mycelia of cultivated Cordyceps were determined. The comparison of extraction solvents and recovery test of the adenosine components were emphasized. The effective extraction-rate by water,as an extraction solvent, was higher than that by 50% alcohol. The addition of 0. 1% sodium azide into water could not only preserve putrefaction,but also make a contribution to the stability of nucleoside components in the liquid sample. When the extraction was carried out by ultrasonic at room temperature,the recovery rate of the added sample was 99. 8%.

Objective:To evaluate the anti-infective therapy in a patient with multi-site infections after brain surgery and explore the role of clinical pharmacist in the treatment. Methods:With the applications of PK/PD characteristics of drugs and the coping strategies of resistant , vancomycin combined with fosfomycin were used to treat the intracranial infection caused by methicillin-resistant Staphylococcus epidermidis with vancomycin MIC of 2 mg·L-1 , and the dynamic adjustment of antibiotics was applied to treat the pulmonary infection caused by two kinds of pathogens including MDR bacteria. Results:The intracranial infection was successfully controlled, the pulmonary infection was also under control, and the overall condition of the patient was significantly improved. Conclusion: In the medical team, clinical pharmacists should make full use of their advantages in drug characteristics to carry out clinical pharmaceutical care.

Objective To assess the influence of transcranial electric stimulation (TES) on the recovery of motor function after cerebral focal ischemia and reperfusion and to explore the mechanisms in terms of neural plasticity.Methods An acute focal ischemia-reperfusion model was established by transient occlusion of the right middle cerebral artery (MCAO).Seventy-two male Sprague-Dawley rats were randomly divided into a TES group,a model group,a sham-operation group and a normal group.The TES group was given TES 24 h after MCAO;the model group received the operation without any treatment.Forelimb placing (FPT) and beam walking (BWT) were mea-sured at the 3rd,7th,14th and 28th day after reperfusion.Microtubule-associated protein-2 (MAP-2) and growth-associated protein-43 (GAP-43) and grey levels of reaction products in the peri-infarct region were examined by immunohistochemical techniques.Results The TES group rats had markedly better FPT and BWT performance at the 7th,14th and 28th day after MCAO,compared with the model group.Expression of MAP-2 had increased significantly more at the 14th and 28th day in the peri-infarct region in the TES group compared with the model group.Expression of GAP-43 was significantly elevated in the peri-infarct region in the TES group compared with the model group at all time points.Conclusions TES can improve motor function and neural plasticity following cerebral ischemia and reperfusion damage.The functional enhancement may be partly due to up-regulation of the expression of GAP-43 and MAP-2 in the peri-infarct region.

Objective To investigate delivery mode of uterine scar pregnancy and analyze the safety of vagi-nal delivery.Methods 210 delivery women with scar uterus after cesarean section and 85 delivery women with non -scar uterus vaginal delivery of maternal selection were collected.According to the different ways of delivery,the preg-nant women in the scar group were divided into A group (72 cases)and B group (138 cases).Delivery outcome, maternal complications and average hospitalization time between the two groups were compared.Results 72 cases of maternal vaginal delivery in scar A group was successful and smooth vaginal delivery in 64 cases,and success rate was 88.89%(64 /72).8 patients maternal modified cesarean section production,accounted for 11.11%(8 /72),including 3 cases of persistent occiput posterior position,2 cases of social factors give up vaginal delivery,2 cases fetal distress, 1 case for incomplete rupture of uterus.138 cases delivery women in scar B group successfully obtained the cesarean section.100 cases of delivery women in non scar group,91 cases successfully got vaginal delivery,and the success rate was 91.00% (91 /100).Another 9 cases of delivery women were replaced with caesarean birth[9.00%(9 /100)],of which 3 cases of fetal distress,2 cases for fetal macrosomia,2 cases of persistent occiput transverse position,2 cases as social factors give up of vaginal delivery.Scar in group A maternal average hospitalization time was significantly shor-ter than scar in group B,and mean postpartum hemorrhage was significantly increased than scar in group B,and the differences were statistically significant (t =15.87,26.32,all P <0.05).There were no significant differences in the incidence of postpartum hemorrhage and neonatal asphyxia between the two groups (χ2 =0.24,1.05,all P >0.05). The maternal average hospitalization time,mean postpartum hemorrhage,postpartum hemorrhage and incidence rate of neonatal asphyxia between scar group A and non scar group had no significant differences (χ2 =0.88,1.30,1.03, 0.00,all P >0.05).Conclusion When choosing vaginal delivery in scar uterus again pregnancy,we should strictly grasp cesarean section pregnancy after vaginal delivery of the indications.Under strict monitoring,the safety of vaginal delivery was high.It was worth of clinical promotion.

OBJECTIVE:To establish a HPLC method for the determination of main component and the related substances in pirenoxinum natricum eye drops. METHODS: A Inertsil C8-3 column was used with pH2.2 perchloric acid-methanol (35∶65) as mobile phase at a flow of 1.0 mL?min-1. The detection wavelength was 230 nm and the sample size was 10 ?L. RESULTS: Pirenoxinum natricum and the related substances could be baseline separated. The calibration curve was linear in the range of 49.5～990 ng(r=0.999 9). The detection limits was 89.3 pg, and average recovery was 99.1%, RSD was 0.42%. CONCLUSIONS: This method is rapid, accurate sensitive and can be used for the quality control of pirenoxinum natricum eye drops.

ObjectiveTo assess how transcranial electrical stimulation (TES) concurrent with rehabilitation training influences brain plasticity and behavioral functional performance in rats following a focal ischemic infarction.MethodsAfter an acute focal ischemic infarction by transient occlusion of right middle cerebral artery (MCAO), the electric stimulation concurrent with rehabilitation group was given TES, balancing and rotating and walking exercise everyday; the rehabilitation group was given only balancing and rotating and walking exercise everyday; the control group received no treatment. Growth associated protein 43(GAP-43) was examined by immunohistochemical techniques, and density of reaction product and forelimb placing test (FPT) were measured on the 3rd, 7th, 14th and 28th day after infarction respectively.ResultsThe electrical stimulation concurrent with rehabilitation group provided marked improvement in FPT on the 7th, 14th and 28th day compared with the rehabilitation group and the control group (P<0.01～0.05). The GAP-43 demonstrated statistically significant increase on the 3rd, 7th, and 14th day in the peri-infarct region in the electrical stimulation concurrent with rehabilitation group compared with other two groups(P<<0.01～0.05).ConclusionThe efficacy of transcranial electrical stimulation combining with rehabilitation training can improve functional outcome and neuronal plasticity following ischemic cerebral damage. The mechanism may be partly due to the upregulation of the expression of GAP-43 in the peri-infarct region. Meanwhile, the efficacy is superior to rehabilitation training only.

50 ms (PNN 50 ) and the high frequency (HF) in patients with EH were decreased, While the ratio between low frequency and high frequency (LF/HF) were increased. After the treatment of irbesartan, the values of SDNN, RMSSD, PNN 50 , and HF were increased, and the LF/HF was decreased significantly. CONCLUSION: Irbersartan can increase the lowed HRV in patients with EH, which improves the cardiovascular automatic function.

Objective To investigate the intervention of trans-theoretical model on the risk factors in the patients with prediabetes.Methods One hundred and twenty-two patients with pre-diabetes in a tertiary hospital of Zhengzhou City were selected and randomly assigned to control group and intervention group.The control group received the routine diabetic health education,while in addition to the routine health education,the intervention group was assisted by TTM interventional measures.The blood glucose,body mass index(BMI) and negative emotion were compared between the two groups.Results The blood glucose and BMI after intervention in the two groups were decreased,moreover the decrease level of fasting blood glucose and BMI in the intervention group was higher than that in the control group,and the difference was statistically significant(t=4.963、2.061,P＜0.05).The anxiety and depression emotion scores after intervention in the intervention group were [(3.47 ±1.07) and (0.30 ± 0.46)],before intervention were (7.74 ± 4.00) and(7.25 ± 4.50),The difference was statistically significant(P＜0.05),Moreover the anxiety and depression emotion scores after intervention in the intervention group were lower tghan those in the control group with statistical difference(t=3.300、2.054,P＜0.05).Conclusion The intervention measures based on TTM has significant advantage for controlling the risk factors of pre-diabetes and preventing diabetes than the conventional health education.