The orientational projections of sensory fibres of median nerve to the Substantia Gelatinosa (SG) of the spinal cord in rats were studied in accordance with the principle of transganglion degeneration by using the acid phosphatase technique. It was found longitudinally that the afferent fibres of the median nerve projected chiefly to SG from the upper part of C_5 to middle part of T_1. In a few rats, projections extended to middle and lower part of C_4 and lower part of T_1. In comparison with the records in anatomy literature and data from researches with HRP technique, a tendency of pre-positioning was noted. The regional projections of the afferent fibres of median nerve were found within the centre line of SG, and mainly in the region of the medial 1/4-1/2 of the medial half. In the segments of C_7-T_1 of a few rats, the whole area of the medial half of SG was projected. In observing the projections of the afferent fibres of median nerve, it was found that they have a tendency of lateralward increase from cranial portion to caudal portion.

The orientational projections of sensory fibres of radial nerve and ulnar nerve to the substantia gelatinosa(SG) of the spinal cord in rats were studied in accordance with the principle of transganglion degeneration by using the acid phosphatase technique.It was found that the affeernt fibers of the radial nerve projected chiefly to SG, from the upper part of C_6 to the middle part of T_1 longitudinally; the ulnar nerves projected chiefly to SG from the middle part of C_6 to the middle part of T_1.In their transverse projections,the afferent fibres of the radial nerve were mainly found in the region of the whole area of the middle 1/3 of SG; and the afferent fibres of ulnar nerve were found within the centre line of SG, and mainly in the region of the lateral 1/2～3/4 at medial half. In summary, radial nerve projected to the lateral region and ulnar nerve to the medial region of SG.

The orientational projections of the sensory fibres of axillary nerve to the substantia gelationsa (SG) of the spinal cord in rats were studied in accordance with the principle of transgangionic degeneration by using acid phosphatase technique. It was found that the afferent fibers of the axillary nerve projected chiefly to SG from the upper part of C_6 to the lower part of C_8 longitudinally. In a few rats, projections extended to the lower part of C_5 and upper part of T_1. In comparison with the available studies, a tendency of backward localization was noted. As to their transverse projections, the afferent fibres of the axillary nerve were found outside of the midline of SG, and mainly located in the lateral half of SG, at the 1/3 of median and the 1/3 of interior region. In the segments of C_7C_8 of some rats, the medial. 1/2 of the lateral half of SG was projected. It was found that the projection column from up downward had a tendency of extension toward lateral portion.

According to content of ‘lipid metabolism’,we crafted a effective case for problem based learning (PBL) and practiced it in biochemistry course.In group discussion,students participated in discussion with great enthusiasm and initiative.During the discussion,students got a better understanding about lipid metabolism and clinical application of the knowledge.As teachers,we maily guided students during PBL,and at the same time,we also summed up the experience and explored how PBL be implemented for freshmen and sophomores who lack clinical practice.

Objective To evaluate the performance of ACL TOP coagulation analyzer system in the laboratory of children′s hospital. Methods According to the documents of CLSI, the analytic characteristics including precision, accuracy, linearity, interference and carryover rate were examined; specimens from healthy children were collected and assayed to determine the reference range of Prothrombin Time (PT), Activated Partial Thromboplastin Time (APTT), Thrombin Time (TT), Fibrinogen (FIB) and D-Dimer (D-D) from children on ACL TOP. Results The within-run and between-day coefficient of variability (CV) were within an acceptable range; The accuracy deviation of PT , APTT and FIB were less than 1/2 allowed total errors; The results of determination of FIB linearity test were correlated with the results of calculation: Y = 1.002 1X-0.122, R2 =0.998 2; The extent of influence of low to middle grade of jaundice , fat and hemolysis on each test were all less than 1/2 allowed total error; The carryover rates were lower than 1.81% and within an acceptable range; The reference range of PT, APTT, TT and FIB were PT (9.1-13.1 s), APTT (24.9-42.1 s), TT (12.6-21.1 s), FIB (1.924-4.011 g/L). Conclusion The ACL TOP coagulation analyzer has good repeatability, stability, linearity and capability of anti-interference and anti-carryover.

AIM To study the effects of PDB on blood glucose. METHODS 3 groups of health mice and 3 groups of model mice were administered with high, mean and low doses of PDB compatibility groups (combined with glibenclamide 0.66 mg?kg -1) respectively for 15 days. The blood goucose variation of health and model mice was observed. RESULTS A low dose of PDB compatibility group decreased obviously blood glucose in the health mice. Its mean value was(3.10?0.14), while control group's blood glucose was 4.46?0.12, P

Objective:To compare the effect of the frequency of transcatheter arterial chemoembolization (TACE) on preventing tumor recurrence after hepatectomy. Methods:A total of 45 post-operative patients who had received prophylactic TACE once or thrice were retrospectively examined between January 2008 and June 2009. Of the 45 patients, 23 underwent TACE once, and the others un-derwent it thrice. TACE was administered to all patients via the hepatic artery one to two months after operation and was repeated every two to four months with patients who underwent TACE three times. All cases were followed up for 36 to 40 months after surgery. The rates of cumulative recurrence between the two groups were compared. Results:In the group that underwent TACE once, the 1-, 2-and 3-year cumulative recurrence rates were 30.43%, 47.83%, and 47.83%, respectively. In the group that underwent TACE thrice, the 1-, 2-and 3-year cumulative recurrence rates were 4.55%, 27.27%, and 36.36%, respectively. Statistical analysis showed that the relapse rate within one year was lower in the group that underwent TACE thrice than in the group that underwent TACE only once (P=0.022). How-ever, no significant difference in the cumulative recurrence rate was found between the two groups in two and three years (P=0.086, 0.225). Conclusion:Hepatocellular carcinoma patients who undergo preventive TACE three times after hepatectomy exhibit reduced re-currence rates during the peak time of tumor recurrence and extended disease-free survival intervals.

OBJECTIVETo explore the effect and mechanism of electroacupuncture (EA) for Z syndrome without organic lesion (metabolic syndrome combined with obstructive sleep apnea hypopnea syndrome).

METHODSFifty-eight patients with Z syndrome were divided into three groups according to mild,moderate and severe degree. Acupuncture and EA were used at Daimai (GB 26), Zhongwan (CV 12), Xiawan (CV 10), Zusanli ST 36), Qihai (CV 6) and Huaroumen (ST 24), etc., once a day and five times a week. The treatment of ten times was a course, and two courses were acquired continuously. Sleep respiration monitoring (PSG) was done before EA and in one week after treatment respectively. Triacylglycerol (TG) fasting blood glucose (FBG) fasting insulin (INS) and serum leptin (Lep) were tested before and after treatment in the three groups.

RESULTSAfter treatment apnea hypopnea index (AHI) and the percentage of the time of arterial oxygen saturation (SaO₂) less than 90% taken in the total sleep time (SLT 90%) were improved apparently than those before treatment in the three groups (all P < 0.05). The levels of TG, FPG, INS and Lep were decreased after treatment in all groups (all P < 0.05).

CONCLUSIONEA can improve AHI and nocturnal hypoxia of Z syndrome, and the mechanism may be related to decreasing the indices of metabolism syndrome and leptin.

Acupuncture Therapy ; Adult ; Female ; Humans ; Insulin ; blood ; Leptin ; blood ; Male ; Metabolic Syndrome ; blood ; physiopathology ; therapy ; Middle Aged ; Sleep ; Sleep Apnea, Obstructive ; blood ; physiopathology ; therapy ; Treatment Outcome ; Triglycerides ; blood ; Young Adult

ObjectiveTo investigate the influences of routine-dose loratadine on the positive patch test reaction to nickel sulfate.MethodsA double-blind,controlled and randomized study was carried out.A total of 121 patients with a positive patch test reaction to nickel sulfate were divided into two groups to receive loratadine 10 mg (experimental group,n =61 ) or placebo (control group,n =60) once daily for 14 days.The patch testing of nickel sulfate was performed on day 11,and clinical evaluation of the test was carried out on day 14 after the start of treatment.The intention to treat population was used for data analysis.ResultsNo changes were observed in the intensity of patch test reaction to nickel sulfate in 55 patients in the experimental group or 53 patients in the control group,and there was no significant difference between the two groups in the percentage of patients showing changes in the intensity of patch test reaction(9.8％ vs.11.7％,x2 =0.11,P ＞ 0.05).ConclusionThe routine-dose loratadine has no inhibitory effect on the intensity of skin patch test reaction to nickel sulfate.

Objective To determinate the heavy metal cadmium content in rice to ensure food safety.Methods Several brands and batch numbers of rice were collected and divided into groups A and B.Group A contained 24 pieces of rice from the canteens,and group B involved in 22 pieces from the farm product markets.Cadmium content in rice was detected quantitatively with X-ray fluorescence spectrometer,and then evaluated according to GB 2762-2012 which determined rice was not qualified in case cadmium content was more than 0.2 mg/kg.Results Group A had cadmium content between 0.00 and 0.477 mg/kg,the times of ultra standard being 2.385 and the disqualification rate being 29.2％ (7/24),and group B had cadmium content between 0.065 and 0.619 mg/kg,the times of ultra standard being 3.095 and the disqualification rate being 68.2％ (15/22).Excessive cadmium content in rice occurred in both canteens and markets,while the canteens was better than the markets.Conclusion X-ray fluorescence spectrometer detects cadmium content in rice rapidly and simply,and is worthy promoting in elementary facilities.