Objective: To study the inhibitory action of Jiangru Power on rat prolactinoma induced by diethylstilbestrol.Methods: Prolactinoma Rat models were prepared by embedding estrogen subcutaneously.Then they were randomly divided into three groups,respectively given intragastric administration of placebo,Jiangru Power and Norprolac.After four weeks of treatment,all the animals were executed.Each pituitary gland was weighed.Serum prolactin(PRL) levels were measured by RIA method.Pituitary tumor transformed gene(PTTG) mRNA levels in pituitary tissue were measured by RT-PCR method.Results: The weight of pituitary gland and serum PRL level in Jiangru Power or Norprolac group all were obviously lower than those in placebo group(P

AIM: To determine whether Jiunaoning injection has protective effects on oxygen/glucose-deprived and reperfusion injury-induced neurons. METHODS: Various concentrations of Jiunaoning injection (0.5-5 mL/L) were used to observe their effects on cultured rat cortical neurons induced by oxygen/glucose-deprived and reperfusion injury in various time points. The neuronal metabolic rate and viability were assessed by using 3-(4,5-dimethylthiazol)-2, 5-diphenyl-tetra zoliumbromide (MTT) and lactate dehydrogenase (LDH) assay. RESULTS: Jiunaoning injection enhanced the neuronal metabolic rate in a dose-dependent manner in the range from 0.5 to 5 mL/L, and Jiunaoning injection (1.5-2.5 mL/L) enhanced the neuronal metabolic rate, decreased the cell death rate and depressed LDH leak rate significantly. CONCLUSION: Jiunaoning injection has an affirmative protective effect on oxygen/glucose-deprived and reperfusion-induced neuronal injury.

Treatment with traditional Chinese medicinal composite is one of the most important characteristics of traditional Chinese medicine (TCM). Studying material base of TCM composite and its mechanism is a key to modernizing the industry of Chinese medicinal herbs. The research for TCM composite can be carried out from many different angles, including multiple components, multiple actions, multiple levels and multiple targets. Such a way to study TCM composite will be beneficial to improving the theory of TCM composite, guiding clinical administration and developing new products.

Objective:To understand the etiology and clinical characteristics of hospitalized severe community-acquired pneumonia(SCAP) in Changchun, and provide scientific basis for its etiology diagnosis and targeted treatment.Methods:The study subjects included 618 children with clinical diagnosis of SCAP who were hospitalized from January 2016 to December 2019.We collected pharyngeal swabs and alveolar lavage fluid from children.Virus isolation, bacterial culture, time-of-flight mass spectrometry, PCR/RT-PCR, colloidal gold method and Optochin test were used to detect the antigen, nucleic acid and protein profiles in the specimen.Results:There were more boys than girls in hospitalized children with SCAP.The peak age of onset was 7 to 12 months.Most cases occurred in winter and spring.The highest detection rate of SCAP virus was 56.15%(347/618); 73.49%(255/347) were positive for one virus, among which the top five were respiratory syncytial virus (27.8%), influenza A virus (23.9%), influenza B virus (16.1%), rhinovirus (12.2%) and metapneumovirus (10.2%). Two viruses were positive for 19.88%(69/347); three viruses were positive for 4.32%(15/347); four viruses were positive for 2.31%(8/347). Atypical microbial infections were 29.77%(184/618), of which Mycoplasma pneumoniae accounted for 95.65%(176/184). Bacterial infections were 17.31%(107/618), mainly Streptococcus pneumoniae(39.25%, 42/107) and Staphylococcus aureus(24.30%, 26/107). The mixed infection of multiple pathogens was 7.61%(47/618), among which the mixed infection rates of Mycoplasma pneumonia with Streptococcus pneumoniae, virus were 40.43% and 34.04%, respectively.High fever, faster breathing, and perioral cyanosis were risk factors for SCAP, with OR and 95% CI of 7.71 and 4.56-13.04, 2.43 and 2.02-2.93, 3.53 and 2.56-4.86, respectively.Viral co-infection occurred in 36.96%(34/92) of complications such as heart failure, toxic encephalopathy, and myocardial damage; Mycoplasma pneumoniae and other pathogens co-infected 35.29% of children with pleural effusion. Conclusion:The pathogens of SCAP in Changchun are mainly viruses notably, respiratory syncytial virus is the dominant pathogen, followed by Mycoplasma pneumoniae.The bacterial pathogen is mainly Streptococcus pneumoniae.High fever, faster breathing, and cyanosis around the mouth are risk factors for severe pneumonia.Multi-pathogen mixed infection is prone to serious complications.

Objective:To explore the clinical curative effect of nifedipine controlled-release tablets combined with telmisartan on diabetes mellitus combined with hypertension and their influences on serum homocysteine (Hcy) and C-reactive protein (CRP).Methods:Eighty-four patients with diabetes mellitus and hypertension who were admitted to Huainan First People′s Hospital from February 2016 to November 2019 were enrolled. They were divided into combination group and control group by random number table method, with 42 cases in each group. The control group was treated with nifedipine controlled-release tablets, while combination group was additionally treated with telmisartan. Both groups were continuously treated for 4 weeks. After treatment, clinical curative effect in both groups was evaluated. The levels of diastolic blood pressure (DBP), systolic blood pressure (SBP), fasting plasma glucose (FPG), 2 h postprandial blood glucose (2 hPG), glycosylated hemoglobin (HbA 1c), total cholesterol (TC), triacylglycerol (TG), Hcy and CRP were measured before and after treatment. The number of cases with adverse reactions was recorded. Results:After treatment, total response rate in combination group was significantly higher than that in control group [92.24%(40/42) vs. 80.95%(34/42)] ( P<0.05). After treatment, there were no significant differences in FPG, 2 hPG and HbA 1c between the two groups ( P>0.05). After treatment, the levels of SBP, DBP, TC, TG, CRP and Hcy in combination group were significantly lower than those in control group [(132.64 ± 7.53) mmHg)(1 mmHg=0.133 kPa) vs. (142.81 ± 4.63) mmHg, (79.63 ± 6.84) mmHg vs.(85.71 ± 5.86) mmHg, (4.87 ± 0.61) mmol/L vs. (5.14 ± 0.62) mmol/L, (1.57 ± 0.41) mmol/L vs.(1.76 ± 0.45) mmol/L,(8.76 ± 1.53) mg/L vs. (9.51 ± 1.86) mg/L, (12.52 ± 1.97) μmol/L vs.(13.48 ± 2.36) μmol/L]( P<0.05). During treatment period, there was no significant difference in incidence of adverse reactions between combination group and control group [16.67% (7/42) vs. 19.05%(8/42)]( P>0.05). Conclusions:The clinical curative effect of nifedipine controlled-release tablets combined with telmisartan is good on diabetes mellitus combined with hypertension, which can regulate blood pressure, blood lipids, Hcy and CRP levels, and relieve disease progression, with good safety.

Objective:To explore the diagnostic value of preoperative serum C-reactive protein(CRP) and thyroid stimulating hormone(TSH) levels in subacute thyroiditis, so as to provide evidence for reducing clinical mistreatment.Methods:From May 2011 to January 2020, the clinical data of 3 525 cases who received thyroid nodule surgery in the First Affiliated Hospital of Anhui University of Science & Technology were retrospectively analyzed.According to the results of pathology, the patients were classified as subacute thyroiditis group, chronic lymphocytic thyroiditis group, thyroid cancer group and benign thyroid nodule group.Fifty-six cases of subacute thyroiditis group(group A) and 56 cases of Hashimoto thyroiditis group(group B) were selected to meet the conditions of the group.Fifty-six cases of thyroid cancer group(group C) and 56 cases of benign thyroid nodule group(group D) were selected according to the random number table method.The serum levels of CRP, TSH and other indicators of each group were counted and analyzed before operation.Results:The misdiagnosis rate of subacute thyroiditis was 1.7%.CRP level in group A was significantly higher than that in groups B, C and D( Z=-3.024, -6.449, -6.012, all P<0.01). The TSH level in group A was significantly lower than that in groups B, C and D( Z=-2.397, -3.877, -2.583, P=0.017, 0.000 and 0.010), while that in group C was significantly higher than group D( Z=-2.421, P=0.016). The area under ROC curve for CRP diagnosis of subacute thyroiditis was 84.3%(95% CI: 0.770-0.917), when the optimal cutoff value was 3.62 mg/L, the diagnostic sensitivity and specificity were 69% and 87%, respectively. Conclusion:Familiarity with clinical manifestations and preoperative serum CRP and TSH levels as differential indicators of benign and malignant thyroid nodules can reduce misdiagnosis of subacute thyroiditis.

Objective To investigate the correlation between the features of conventional ultrasound& shear wave elasticity and axillary lymph node involvement in breast cancer . Methods A total of 169 breast cancers patients were divided into lymph node metastasis group( n = 115) and non metastasis group ( n = 54 ) according to the postoperative pathological results . Preoperative conventional ultrasonographic features and preoperative shear wave elastography quantitative parameters ( E values ) of the two groups breast lessons were analyzed by single factor analysis to screen out statistically significant factors ,then Logistic regression analysis was performed to analyze the relationship between above factors and lymph node involvement . Results Single factor analysis showed the microcalcification and hyperechoic halo detection rates of lymph node metastasis group [ 81 .7％ ( 94/115) and 71 .3％ ( 82/115 ) ,respectively] were higher than those in non metastasis group [ 61 .1％ (33/54) and 50 .0％ ( 27/54) ,respectively] . The elastography maximum value( Emax) of lymph node involvement group was ( 182 .2 ± 74 .0) kPa ,which was larger than that in non metastasis group′s ( 153 .3 ± 76 .9) kPa ( P < 0 .05) . Multivariate Logistic regression analysis showed the microcalcification( OR = 2 .498 , P = 0 .022) ,the hyperechoic halo( OR = 2 .482 , P = 0 .013) and the Emax value( OR = 1 .007 , P = 0 .007) were risk factors of axillary lymph node metastasis in breast cancer . Conclusions Breast cancer with microcalcification ,hyperechoic signs and high Emax value is more likely to develop axillary lymph node metastasis .

Overexpressing of ATP-binding cassette (ABC) transporters is the essential cause of multidrug resistance (MDR), which is a significant hurdle to the success of chemotherapy in many cancers. Therefore, inhibiting the activity of ABC transporters may be a logical approach to circumvent MDR. Olmutinib is an epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), which has been approved in South Korea for advanced EGFR T790M-positive non-small cell lung cancer (NSCLC). Here, we found that olmutinib significantly increased the sensitivity of chemotherapy drug in ABCG2-overexpressing cells. Furthermore, olmutinib could also increase the retention of doxorubicin (DOX) and rhodamine 123 (Rho 123) in ABC transporter subfamily G member 2 (ABCG2)-overexpressing cells. In addition, olmutinib was found to stimulate ATPase activity and inhibit photolabeling of ABCG2 with [I]-iodoarylazidoprazosin (IAAP). However, olmutinib neither altered ABCG2 expression at protein and mRNA levels nor blocked EGFR, Her-2 downstream signaling of AKT and ERK. Importantly, olmutinib enhanced the efficacy of topotecan on the inhibition of S1-MI-80 cell xenograft growth. All the results suggest that olmutinib reverses ABCG2-mediated MDR by binding to ATP bind site of ABCG2 and increasing intracellular chemotherapeutic drug accumulation. Our findings encouraged to further clinical investigation on combination therapy of olmutinib with conventional chemotherapeutic drugs in ABCG2-overexpressing cancer patients.

Secalonic acid D (SAD) could inhibit cell growth in not only sensitive cells but also multidrug resistant (MDR) cells. However, the molecular mechanisms need to be elucidated. Here, we identified that SAD possessed potent cytotoxicity in 3 pairs of MDR and their parental sensitive cells including S1-MI-80 and S1, H460/MX20 and H460, MCF-7/ADR and MCF-7 cells. Furthermore, SAD induced cell G2/M phase arrest the downregulation of cyclin B1 and the increase of CDC2 phosphorylation. Importantly, JNK pathway upregulated the expression of c-Jun in protein level and increased c-Jun phosphorylation induced by SAD, which was linked to cell apoptosis c-Jun/Src/STAT3 pathway. To investigate the mechanisms of upregulation of c-Jun protein by SAD, the mRNA expression level and degradation of c-Jun were examined. We found that SAD did not alter the mRNA level of c-Jun but inhibited its proteasome-dependent degradation. Taken together, these results implicate that SAD induces cancer cell death through c-Jun/Src/STAT3 signaling axis by inhibiting the proteasome-dependent degradation of c-Jun in both sensitive cells and ATP-binding cassette transporter sub-family G member 2 (ABCG2)-mediated MDR cells.