Objective To examine the changes of matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of metalloproteinase-1 (TIMP-1) in gingival crevicular fluid after conventional mechanical therapy of adult patients with chronic periodontitis. Methods Gingival crevicular fluid samples from 15 patients and 15 healthy subjects were taken with filter paper strips by intra-pocket method initially, respectively, 1 month after scaling and root planning. MMP-9,TIMP-1 levels were measured by immunoblotting. Results MMP-9 levels in patients were redueed significantly after treatment[(2.2±1.4)AU vs (1.2±0.9)AU ]. The clinical parameters were positive correlation with MMP-9 level. TIMP-1 levels in patients were increased significantly after treatment[(1.3±1.2)AU vs (3.7±2.2)AU]. The clinical parameters were negative torrelation with TIMP-1 level. Conclusion MMP-9, TIMP-1 levels can act as objective parameters to evaluate the effect of periodontal therapy.

Objective To develop a clinically applicable approach to enhance repair of cartilage defects by constructing an in vivo non-viral gene transfer system targeting chondrocytes. Methods High molecular weight chitosan (HMWC) was degraded to produce low molecular weight chitosan (LM-WC) that was combined with transforming growth factor-β1 (TGF-β1) plasmid to form stable nano-sizc complexes. After being tested in vitro firstly, these nano-size complexes were injected into the knee joint of New Zealand white rabbit models with full-thickness cartilage defects to detect their feasibility of delive-ring the growth factor gent in vivo. Results The results showed that LMWC/DNA nano-sizc comple-xes could deliver the gone into the cultured chondroeytes and cartilage tissue efficiently in vitro. When used in vivo, LMWC/TGF-β1 gene nano-size complexes could enhance the transfection efficiency and prolong the expression of TGF-β1 gone. In the animal models of articular osteechondral defect of rabbits, better healing and gentler degeneration could be observed in comparison with the control. Conclusion In vivo transfection of LMWC/TGF-β1 nano-size complexe is a safe and effective method to early promote the repair of osteochondral defects.

Objective: To investigate the ultrasonographic features of fibrocystic breast change (FBC) and improve the ultrasonographic diagnosis and differential diagnosis of FBC. Methods: Fifty-five patients of FBC with 60 lesions and 39 patients of invasive ductal carcinoma (IDC) with 42 lesions, which were confirmed by pathology after operation in the Affiliated Union Hospital of Fujian Medical University from January 2014 to February 2019 were enrolled. The preoperative sonographic findings of FBC and IDC were retrospectively analyzed and compared. Results: There was no significant difference between the two groups in the rates of showing irregular shape, which were 86.7% in FBC group and 88.1% in IDC group, respectively (P>0.05), but the rate of showing crab feet or burrs on the edge of lesions in FBC group was lower than that in IDC group(P<0.05). The occurrence rates of posterior echo enhancement and cystic degeneration in FBC group were 81.7% and 71.7% respectively, which were significantly higher than those in IDC group (38.1% and 16.7%)(P<0.001). In addition, the FBC group showed fewer features such as hyperechoic halo and more features such as hypovascular supply than that of IDC group (all P<0.001). Conclusions In terms of ultrasonic features, including irregular shape or even crab feet and burrs, FBC can be easily misdiagnosed as malignant tumors. However, posterior echo enhancement, interior scattered small cysts, lack of blood supply and rare hyperechoic halo may be the characteristics of FBC, which can be differentiated from malignant tumors.

Objective To investigate the ultrasonographic features of fibrocystic breast change ( FBC) and improve the ultrasonographic diagnosis and differential diagnosis of FBC . Methods Fifty‐five patients of FBC with 60 lesions and 39 patients of invasive ductal carcinoma ( IDC ) with 42 lesions ,which were confirmed by pathology after operation in the Affiliated Union Hospital of Fujian M edical University from January 2014 to February 2019 were enrolled . T he preoperative sonographic findings of FBC and IDC were retrospectively analyzed and compared . Results T here was no significant difference between the two groups in the rates of showing irregular shape ,which were 86 .7% in FBC group and 88 .1% in IDC group , respectively ( P ＞0 .05) ,but the rate of showing crab feet or burrs on the edge of lesions in FBC group was lower than that in IDC group( P ＜0 .05 ) . T he occurrence rates of posterior echo enhancement and cystic degeneration in FBC group were 81 .7% and 71 .7% respectively ,w hich were significantly higher than those in IDC group ( 38 .1% and 16 .7% ) ( P ＜0 .001) . In addition ,the FBC group showed fewer features such as hyperechoic halo and more features such as hypovascular supply than that of IDC group ( all P ＜0 .001 ) . Conclusions In terms of ultrasonic features ,including irregular shape or even crab feet and burrs ,FBC can be easily misdiagnosed as malignant tumors . However ,posterior echo enhancement ,interior scattered small cysts ,lack of blood supply and rare hyperechoic halo may be the characteristics of FBC ,w hich can be differentiated from malignant tumors .

Arsenic trioxide (ATO) is used as a chemotherapeutic agent for the treatment of acute promyelocytic leukemia. However, increasing drug resistance is reducing its efficacy. Therefore, a better understanding of ATO resistance mechanism is required. In this study, we established an ATO-resistant human epidermoid carcinoma cell line, KB/ATO, from its parental KB-3-1 cells. In addition to ATO, KB/ATO cells also exhibited cross-resistance to other anticancer drugs such as cisplatin, antimony potassium tartrate, and 6-mercaptopurine. The arsenic accumulation in KB/ATO cells was significantly lower than that in KB-3-1 cells. Further analysis indicated that neither application of P-glycoprotein inhibitor, breast cancer resistant protein (BCRP) inhibitor, or multidrug resistance protein 1 (MRP1) inhibitor could eliminate ATO resistance. We found that the expression level of ABCB6 was increased in KB/ATO cells. In conclusion, ABCB6 could be an important factor for ATO resistance in KB/ATO cells. The ABCB6 level may serve as a predictive biomarker for the effectiveness of ATO therapy.